Amino acid dipepetide frequency for Bifidobacterium simiarum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.338AlaAla: 13.338 ± 0.205	1.012AlaCys: 1.012 ± 0.036	7.913AlaAsp: 7.913 ± 0.144	6.528AlaGlu: 6.528 ± 0.119	3.482AlaPhe: 3.482 ± 0.066	9.553AlaGly: 9.553 ± 0.141	2.04AlaHis: 2.04 ± 0.06	5.456AlaIle: 5.456 ± 0.095	4.768AlaLys: 4.768 ± 0.083	9.72AlaLeu: 9.72 ± 0.159	3.165AlaMet: 3.165 ± 0.071	3.361AlaAsn: 3.361 ± 0.073	3.913AlaPro: 3.913 ± 0.076	3.825AlaGln: 3.825 ± 0.087	6.226AlaArg: 6.226 ± 0.101	6.502AlaSer: 6.502 ± 0.126	5.708AlaThr: 5.708 ± 0.101	9.017AlaVal: 9.017 ± 0.123	1.406AlaTrp: 1.406 ± 0.043	2.72AlaTyr: 2.72 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	1.025CysAla: 1.025 ± 0.042	0.112CysCys: 0.112 ± 0.012	0.565CysAsp: 0.565 ± 0.03	0.466CysGlu: 0.466 ± 0.026	0.313CysPhe: 0.313 ± 0.021	0.94CysGly: 0.94 ± 0.043	0.198CysHis: 0.198 ± 0.015	0.43CysIle: 0.43 ± 0.025	0.206CysLys: 0.206 ± 0.017	0.796CysLeu: 0.796 ± 0.034	0.234CysMet: 0.234 ± 0.021	0.217CysAsn: 0.217 ± 0.017	0.429CysPro: 0.429 ± 0.025	0.201CysGln: 0.201 ± 0.016	0.611CysArg: 0.611 ± 0.028	0.495CysSer: 0.495 ± 0.028	0.479CysThr: 0.479 ± 0.026	0.747CysVal: 0.747 ± 0.033	0.108CysTrp: 0.108 ± 0.012	0.228CysTyr: 0.228 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	7.028AspAla: 7.028 ± 0.112	0.539AspCys: 0.539 ± 0.03	5.246AspAsp: 5.246 ± 0.105	4.913AspGlu: 4.913 ± 0.094	2.301AspPhe: 2.301 ± 0.051	6.21AspGly: 6.21 ± 0.103	1.59AspHis: 1.59 ± 0.045	3.062AspIle: 3.062 ± 0.063	2.041AspLys: 2.041 ± 0.058	5.707AspLeu: 5.707 ± 0.099	1.608AspMet: 1.608 ± 0.048	1.535AspAsn: 1.535 ± 0.047	3.891AspPro: 3.891 ± 0.077	1.899AspGln: 1.899 ± 0.064	4.563AspArg: 4.563 ± 0.112	3.523AspSer: 3.523 ± 0.081	3.17AspThr: 3.17 ± 0.074	5.452AspVal: 5.452 ± 0.101	0.993AspTrp: 0.993 ± 0.038	1.925AspTyr: 1.925 ± 0.052	0.0AspXaa: 0.0 ± 0.0
Glu	6.081GluAla: 6.081 ± 0.116	0.45GluCys: 0.45 ± 0.025	3.12GluAsp: 3.12 ± 0.073	3.322GluGlu: 3.322 ± 0.08	1.904GluPhe: 1.904 ± 0.052	4.066GluGly: 4.066 ± 0.076	1.75GluHis: 1.75 ± 0.048	2.884GluIle: 2.884 ± 0.068	2.07GluLys: 2.07 ± 0.065	5.053GluLeu: 5.053 ± 0.094	1.383GluMet: 1.383 ± 0.042	1.711GluAsn: 1.711 ± 0.052	2.579GluPro: 2.579 ± 0.068	2.325GluGln: 2.325 ± 0.061	4.49GluArg: 4.49 ± 0.101	3.418GluSer: 3.418 ± 0.079	3.451GluThr: 3.451 ± 0.07	3.57GluVal: 3.57 ± 0.085	0.703GluTrp: 0.703 ± 0.035	1.743GluTyr: 1.743 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	4.006PheAla: 4.006 ± 0.093	0.389PheCys: 0.389 ± 0.023	2.719PheAsp: 2.719 ± 0.064	1.746PheGlu: 1.746 ± 0.052	1.238PhePhe: 1.238 ± 0.044	3.542PheGly: 3.542 ± 0.084	0.719PheHis: 0.719 ± 0.033	1.707PheIle: 1.707 ± 0.063	0.943PheLys: 0.943 ± 0.041	2.72PheLeu: 2.72 ± 0.078	0.79PheMet: 0.79 ± 0.04	1.194PheAsn: 1.194 ± 0.046	1.499PhePro: 1.499 ± 0.05	0.81PheGln: 0.81 ± 0.036	1.833PheArg: 1.833 ± 0.053	2.162PheSer: 2.162 ± 0.05	2.078PheThr: 2.078 ± 0.061	2.76PheVal: 2.76 ± 0.072	0.446PheTrp: 0.446 ± 0.026	0.931PheTyr: 0.931 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	7.567GlyAla: 7.567 ± 0.122	0.735GlyCys: 0.735 ± 0.028	5.037GlyAsp: 5.037 ± 0.088	4.897GlyGlu: 4.897 ± 0.087	3.423GlyPhe: 3.423 ± 0.071	6.669GlyGly: 6.669 ± 0.122	1.903GlyHis: 1.903 ± 0.058	4.792GlyIle: 4.792 ± 0.083	3.624GlyLys: 3.624 ± 0.081	7.351GlyLeu: 7.351 ± 0.111	2.297GlyMet: 2.297 ± 0.063	2.762GlyAsn: 2.762 ± 0.074	2.783GlyPro: 2.783 ± 0.072	2.568GlyGln: 2.568 ± 0.061	5.566GlyArg: 5.566 ± 0.091	5.647GlySer: 5.647 ± 0.121	5.448GlyThr: 5.448 ± 0.118	6.829GlyVal: 6.829 ± 0.1	1.366GlyTrp: 1.366 ± 0.047	2.78GlyTyr: 2.78 ± 0.075	0.0GlyXaa: 0.0 ± 0.0
His	2.224HisAla: 2.224 ± 0.067	0.188HisCys: 0.188 ± 0.016	1.726HisAsp: 1.726 ± 0.048	1.28HisGlu: 1.28 ± 0.048	0.648HisPhe: 0.648 ± 0.029	2.078HisGly: 2.078 ± 0.055	0.606HisHis: 0.606 ± 0.03	1.08HisIle: 1.08 ± 0.043	0.561HisLys: 0.561 ± 0.026	1.651HisLeu: 1.651 ± 0.044	0.565HisMet: 0.565 ± 0.028	0.675HisAsn: 0.675 ± 0.04	1.347HisPro: 1.347 ± 0.034	0.607HisGln: 0.607 ± 0.031	1.514HisArg: 1.514 ± 0.052	0.993HisSer: 0.993 ± 0.036	1.25HisThr: 1.25 ± 0.04	1.867HisVal: 1.867 ± 0.051	0.305HisTrp: 0.305 ± 0.022	0.647HisTyr: 0.647 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.522IleAla: 6.522 ± 0.107	0.565IleCys: 0.565 ± 0.028	4.186IleAsp: 4.186 ± 0.081	3.066IleGlu: 3.066 ± 0.072	1.437IlePhe: 1.437 ± 0.052	4.923IleGly: 4.923 ± 0.099	0.968IleHis: 0.968 ± 0.035	2.805IleIle: 2.805 ± 0.09	1.618IleLys: 1.618 ± 0.055	3.821IleLeu: 3.821 ± 0.088	1.2IleMet: 1.2 ± 0.04	1.716IleAsn: 1.716 ± 0.053	2.696IlePro: 2.696 ± 0.052	1.186IleGln: 1.186 ± 0.039	3.19IleArg: 3.19 ± 0.074	2.953IleSer: 2.953 ± 0.072	3.201IleThr: 3.201 ± 0.077	4.744IleVal: 4.744 ± 0.092	0.614IleTrp: 0.614 ± 0.031	1.108IleTyr: 1.108 ± 0.041	0.0IleXaa: 0.0 ± 0.0
Lys	4.715LysAla: 4.715 ± 0.095	0.162LysCys: 0.162 ± 0.015	2.385LysAsp: 2.385 ± 0.071	1.972LysGlu: 1.972 ± 0.065	0.925LysPhe: 0.925 ± 0.036	2.679LysGly: 2.679 ± 0.075	0.74LysHis: 0.74 ± 0.032	1.691LysIle: 1.691 ± 0.049	1.538LysLys: 1.538 ± 0.055	2.879LysLeu: 2.879 ± 0.064	0.823LysMet: 0.823 ± 0.04	1.252LysAsn: 1.252 ± 0.043	2.177LysPro: 2.177 ± 0.064	1.218LysGln: 1.218 ± 0.044	2.299LysArg: 2.299 ± 0.067	2.152LysSer: 2.152 ± 0.061	2.556LysThr: 2.556 ± 0.074	2.808LysVal: 2.808 ± 0.062	0.469LysTrp: 0.469 ± 0.025	1.017LysTyr: 1.017 ± 0.04	0.0LysXaa: 0.0 ± 0.0
Leu	10.234LeuAla: 10.234 ± 0.143	0.94LeuCys: 0.94 ± 0.036	6.034LeuAsp: 6.034 ± 0.109	4.291LeuGlu: 4.291 ± 0.083	3.072LeuPhe: 3.072 ± 0.085	7.342LeuGly: 7.342 ± 0.11	1.889LeuHis: 1.889 ± 0.05	4.639LeuIle: 4.639 ± 0.095	3.228LeuLys: 3.228 ± 0.069	7.904LeuLeu: 7.904 ± 0.14	2.306LeuMet: 2.306 ± 0.053	2.991LeuAsn: 2.991 ± 0.066	4.446LeuPro: 4.446 ± 0.082	2.017LeuGln: 2.017 ± 0.055	5.543LeuArg: 5.543 ± 0.103	5.294LeuSer: 5.294 ± 0.094	5.615LeuThr: 5.615 ± 0.091	6.382LeuVal: 6.382 ± 0.114	1.007LeuTrp: 1.007 ± 0.04	2.184LeuTyr: 2.184 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	2.955MetAla: 2.955 ± 0.068	0.218MetCys: 0.218 ± 0.016	1.329MetAsp: 1.329 ± 0.04	1.084MetGlu: 1.084 ± 0.045	0.889MetPhe: 0.889 ± 0.034	1.832MetGly: 1.832 ± 0.054	0.509MetHis: 0.509 ± 0.026	1.558MetIle: 1.558 ± 0.051	0.924MetLys: 0.924 ± 0.031	2.544MetLeu: 2.544 ± 0.061	0.775MetMet: 0.775 ± 0.03	0.876MetAsn: 0.876 ± 0.038	1.387MetPro: 1.387 ± 0.045	0.756MetGln: 0.756 ± 0.035	1.779MetArg: 1.779 ± 0.052	1.724MetSer: 1.724 ± 0.047	2.064MetThr: 2.064 ± 0.055	1.887MetVal: 1.887 ± 0.045	0.274MetTrp: 0.274 ± 0.019	0.551MetTyr: 0.551 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.536AsnAla: 3.536 ± 0.072	0.226AsnCys: 0.226 ± 0.017	2.117AsnAsp: 2.117 ± 0.062	1.674AsnGlu: 1.674 ± 0.052	0.884AsnPhe: 0.884 ± 0.035	3.208AsnGly: 3.208 ± 0.092	0.696AsnHis: 0.696 ± 0.032	1.542AsnIle: 1.542 ± 0.05	1.04AsnLys: 1.04 ± 0.043	2.618AsnLeu: 2.618 ± 0.062	0.7AsnMet: 0.7 ± 0.028	0.949AsnAsn: 0.949 ± 0.045	2.264AsnPro: 2.264 ± 0.07	0.917AsnGln: 0.917 ± 0.037	2.104AsnArg: 2.104 ± 0.057	1.539AsnSer: 1.539 ± 0.055	1.929AsnThr: 1.929 ± 0.059	2.415AsnVal: 2.415 ± 0.063	0.435AsnTrp: 0.435 ± 0.025	0.764AsnTyr: 0.764 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	5.124ProAla: 5.124 ± 0.099	0.33ProCys: 0.33 ± 0.021	3.636ProAsp: 3.636 ± 0.081	3.331ProGlu: 3.331 ± 0.074	1.606ProPhe: 1.606 ± 0.051	3.644ProGly: 3.644 ± 0.07	1.013ProHis: 1.013 ± 0.038	2.343ProIle: 2.343 ± 0.056	1.975ProLys: 1.975 ± 0.061	3.742ProLeu: 3.742 ± 0.081	1.105ProMet: 1.105 ± 0.041	1.611ProAsn: 1.611 ± 0.051	1.236ProPro: 1.236 ± 0.047	1.678ProGln: 1.678 ± 0.049	2.443ProArg: 2.443 ± 0.061	2.987ProSer: 2.987 ± 0.069	2.959ProThr: 2.959 ± 0.068	4.016ProVal: 4.016 ± 0.076	0.763ProTrp: 0.763 ± 0.036	1.454ProTyr: 1.454 ± 0.049	0.0ProXaa: 0.0 ± 0.0
Gln	3.494GlnAla: 3.494 ± 0.073	0.26GlnCys: 0.26 ± 0.021	1.393GlnAsp: 1.393 ± 0.045	1.391GlnGlu: 1.391 ± 0.045	0.988GlnPhe: 0.988 ± 0.04	2.173GlnGly: 2.173 ± 0.061	0.668GlnHis: 0.668 ± 0.026	1.887GlnIle: 1.887 ± 0.055	1.004GlnLys: 1.004 ± 0.044	2.92GlnLeu: 2.92 ± 0.075	0.826GlnMet: 0.826 ± 0.033	0.904GlnAsn: 0.904 ± 0.035	1.543GlnPro: 1.543 ± 0.059	1.345GlnGln: 1.345 ± 0.059	2.222GlnArg: 2.222 ± 0.06	2.15GlnSer: 2.15 ± 0.075	2.001GlnThr: 2.001 ± 0.063	2.201GlnVal: 2.201 ± 0.055	0.555GlnTrp: 0.555 ± 0.027	0.977GlnTyr: 0.977 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	5.646ArgAla: 5.646 ± 0.107	0.498ArgCys: 0.498 ± 0.027	3.895ArgAsp: 3.895 ± 0.083	3.911ArgGlu: 3.911 ± 0.076	2.533ArgPhe: 2.533 ± 0.063	4.303ArgGly: 4.303 ± 0.085	1.736ArgHis: 1.736 ± 0.054	3.847ArgIle: 3.847 ± 0.072	2.509ArgLys: 2.509 ± 0.057	6.13ArgLeu: 6.13 ± 0.121	2.098ArgMet: 2.098 ± 0.064	2.201ArgAsn: 2.201 ± 0.062	2.883ArgPro: 2.883 ± 0.081	2.307ArgGln: 2.307 ± 0.062	5.696ArgArg: 5.696 ± 0.139	3.821ArgSer: 3.821 ± 0.08	3.694ArgThr: 3.694 ± 0.082	4.25ArgVal: 4.25 ± 0.071	0.924ArgTrp: 0.924 ± 0.037	2.084ArgTyr: 2.084 ± 0.056	0.0ArgXaa: 0.0 ± 0.0
Ser	6.819SerAla: 6.819 ± 0.119	0.477SerCys: 0.477 ± 0.029	3.833SerAsp: 3.833 ± 0.082	2.923SerGlu: 2.923 ± 0.071	2.12SerPhe: 2.12 ± 0.06	6.217SerGly: 6.217 ± 0.126	1.298SerHis: 1.298 ± 0.046	2.972SerIle: 2.972 ± 0.063	2.137SerLys: 2.137 ± 0.067	5.282SerLeu: 5.282 ± 0.096	1.626SerMet: 1.626 ± 0.047	1.763SerAsn: 1.763 ± 0.06	2.624SerPro: 2.624 ± 0.062	1.987SerGln: 1.987 ± 0.061	3.702SerArg: 3.702 ± 0.09	4.425SerSer: 4.425 ± 0.14	3.598SerThr: 3.598 ± 0.083	4.773SerVal: 4.773 ± 0.1	0.872SerTrp: 0.872 ± 0.037	1.542SerTyr: 1.542 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	6.661ThrAla: 6.661 ± 0.131	0.455ThrCys: 0.455 ± 0.029	4.076ThrAsp: 4.076 ± 0.084	2.887ThrGlu: 2.887 ± 0.066	2.128ThrPhe: 2.128 ± 0.059	5.395ThrGly: 5.395 ± 0.102	1.136ThrHis: 1.136 ± 0.039	3.366ThrIle: 3.366 ± 0.077	2.039ThrLys: 2.039 ± 0.051	5.612ThrLeu: 5.612 ± 0.104	1.494ThrMet: 1.494 ± 0.046	1.901ThrAsn: 1.901 ± 0.058	3.413ThrPro: 3.413 ± 0.069	1.748ThrGln: 1.748 ± 0.053	2.992ThrArg: 2.992 ± 0.067	3.508ThrSer: 3.508 ± 0.081	3.583ThrThr: 3.583 ± 0.082	5.779ThrVal: 5.779 ± 0.125	0.706ThrTrp: 0.706 ± 0.03	1.546ThrTyr: 1.546 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	8.436ValAla: 8.436 ± 0.126	0.835ValCys: 0.835 ± 0.039	5.198ValAsp: 5.198 ± 0.096	4.225ValGlu: 4.225 ± 0.095	2.804ValPhe: 2.804 ± 0.076	5.584ValGly: 5.584 ± 0.092	1.454ValHis: 1.454 ± 0.045	4.639ValIle: 4.639 ± 0.097	2.875ValLys: 2.875 ± 0.07	7.078ValLeu: 7.078 ± 0.115	1.917ValMet: 1.917 ± 0.054	2.55ValAsn: 2.55 ± 0.066	4.089ValPro: 4.089 ± 0.072	2.053ValGln: 2.053 ± 0.054	4.921ValArg: 4.921 ± 0.102	5.406ValSer: 5.406 ± 0.089	5.305ValThr: 5.305 ± 0.119	6.416ValVal: 6.416 ± 0.107	0.98ValTrp: 0.98 ± 0.035	2.085ValTyr: 2.085 ± 0.06	0.001ValXaa: 0.001 ± 0.002
Trp	1.161TrpAla: 1.161 ± 0.047	0.133TrpCys: 0.133 ± 0.013	0.78TrpAsp: 0.78 ± 0.035	0.578TrpGlu: 0.578 ± 0.03	0.554TrpPhe: 0.554 ± 0.025	0.985TrpGly: 0.985 ± 0.041	0.321TrpHis: 0.321 ± 0.019	0.714TrpIle: 0.714 ± 0.032	0.581TrpLys: 0.581 ± 0.027	1.397TrpLeu: 1.397 ± 0.047	0.409TrpMet: 0.409 ± 0.023	0.535TrpAsn: 0.535 ± 0.026	0.551TrpPro: 0.551 ± 0.03	0.574TrpGln: 0.574 ± 0.031	1.165TrpArg: 1.165 ± 0.048	0.823TrpSer: 0.823 ± 0.038	0.807TrpThr: 0.807 ± 0.034	0.832TrpVal: 0.832 ± 0.037	0.257TrpTrp: 0.257 ± 0.017	0.466TrpTyr: 0.466 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.084TyrAla: 3.084 ± 0.066	0.277TyrCys: 0.277 ± 0.02	2.011TyrAsp: 2.011 ± 0.055	1.588TyrGlu: 1.588 ± 0.051	0.984TyrPhe: 0.984 ± 0.044	2.7TyrGly: 2.7 ± 0.07	0.577TyrHis: 0.577 ± 0.025	1.106TyrIle: 1.106 ± 0.04	0.779TyrLys: 0.779 ± 0.034	2.431TyrLeu: 2.431 ± 0.061	0.594TyrMet: 0.594 ± 0.027	0.853TyrAsn: 0.853 ± 0.04	1.245TyrPro: 1.245 ± 0.043	0.88TyrGln: 0.88 ± 0.041	2.105TyrArg: 2.105 ± 0.064	1.465TyrSer: 1.465 ± 0.048	1.527TyrThr: 1.527 ± 0.057	2.106TyrVal: 2.106 ± 0.055	0.438TyrTrp: 0.438 ± 0.025	0.863TyrTyr: 0.863 ± 0.039	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.002	0.0XaaTyr: 0.0 ± 0.0	0.005XaaXaa: 0.005 ± 0.004

Statistics based on 2025 proteins (751035 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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