Streptococcus phage Javan502

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BB85|A0A4D6BB85_9CAUD Uncharacterized protein OS=Streptococcus phage Javan502 OX=2548216 GN=Javan502_0035 PE=4 SV=1
MM1 pKa = 7.44SIVIPTLEE9 pKa = 5.02GDD11 pKa = 3.76MVAQKK16 pKa = 10.66SDD18 pKa = 3.96YY19 pKa = 9.81IIKK22 pKa = 10.55GIAGEE27 pKa = 4.56FYY29 pKa = 10.39SCKK32 pKa = 9.73PDD34 pKa = 3.46VFEE37 pKa = 4.09QTYY40 pKa = 10.64EE41 pKa = 4.11STT43 pKa = 3.65

Molecular weight: 4.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BC74|A0A4D6BC74_9CAUD Hyaluronate lyase OS=Streptococcus phage Javan502 OX=2548216 GN=Javan502_0010 PE=4 SV=1
MM1 pKa = 7.36TKK3 pKa = 7.85QHH5 pKa = 6.91RR6 pKa = 11.84EE7 pKa = 3.47TLIWYY12 pKa = 7.66RR13 pKa = 11.84ASHH16 pKa = 5.21QEE18 pKa = 3.99RR19 pKa = 11.84EE20 pKa = 4.0RR21 pKa = 11.84LLDD24 pKa = 4.04FGLVDD29 pKa = 3.46KK30 pKa = 11.12ARR32 pKa = 11.84YY33 pKa = 7.09VTLLRR38 pKa = 11.84QLRR41 pKa = 11.84KK42 pKa = 9.91KK43 pKa = 10.48YY44 pKa = 10.73AII46 pKa = 4.1

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

10574

43

785

203.3

22.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.405 ± 0.54

0.671 ± 0.123

6.582 ± 0.335

7.036 ± 0.527

3.953 ± 0.182

6.554 ± 0.472

1.466 ± 0.157

7.131 ± 0.258

9.448 ± 0.449

8.294 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.18

5.183 ± 0.248

2.818 ± 0.219

3.887 ± 0.218

3.726 ± 0.326

6.459 ± 0.403

5.892 ± 0.224

6.336 ± 0.248

1.106 ± 0.131

3.698 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski