Amino acid dipepetide frequency for Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.667AlaAla: 4.667 ± 1.55
0.933AlaCys: 0.933 ± 0.262
4.667AlaAsp: 4.667 ± 1.472
2.334AlaGlu: 2.334 ± 1.06
1.867AlaPhe: 1.867 ± 0.676
1.867AlaGly: 1.867 ± 0.645
0.933AlaHis: 0.933 ± 0.454
1.867AlaIle: 1.867 ± 0.412
2.1AlaLys: 2.1 ± 0.811
3.967AlaLeu: 3.967 ± 0.885
1.167AlaMet: 1.167 ± 0.53
1.634AlaAsn: 1.634 ± 0.806
3.967AlaPro: 3.967 ± 1.926
1.867AlaGln: 1.867 ± 0.305
2.8AlaArg: 2.8 ± 0.558
3.734AlaSer: 3.734 ± 1.01
2.8AlaThr: 2.8 ± 0.493
4.201AlaVal: 4.201 ± 1.315
0.233AlaTrp: 0.233 ± 0.333
1.867AlaTyr: 1.867 ± 0.267
0.0AlaXaa: 0.0 ± 0.0
Cys
0.933CysAla: 0.933 ± 0.552
0.233CysCys: 0.233 ± 0.15
1.167CysAsp: 1.167 ± 0.681
0.467CysGlu: 0.467 ± 0.312
0.467CysPhe: 0.467 ± 0.217
0.7CysGly: 0.7 ± 0.298
0.467CysHis: 0.467 ± 0.393
0.933CysIle: 0.933 ± 0.262
3.267CysLys: 3.267 ± 1.182
1.167CysLeu: 1.167 ± 0.306
0.0CysMet: 0.0 ± 0.0
0.467CysAsn: 0.467 ± 0.3
1.867CysPro: 1.867 ± 0.976
1.167CysGln: 1.167 ± 0.306
0.933CysArg: 0.933 ± 0.6
0.7CysSer: 0.7 ± 0.298
0.933CysThr: 0.933 ± 0.346
0.7CysVal: 0.7 ± 0.318
0.467CysTrp: 0.467 ± 0.3
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
2.1AspAla: 2.1 ± 0.647
0.233AspCys: 0.233 ± 0.311
4.434AspAsp: 4.434 ± 0.692
4.667AspGlu: 4.667 ± 1.425
2.8AspPhe: 2.8 ± 0.481
5.134AspGly: 5.134 ± 1.187
1.867AspHis: 1.867 ± 0.8
3.734AspIle: 3.734 ± 0.734
3.967AspLys: 3.967 ± 0.885
7.001AspLeu: 7.001 ± 1.207
1.4AspMet: 1.4 ± 0.632
2.567AspAsn: 2.567 ± 0.9
2.567AspPro: 2.567 ± 0.641
1.4AspGln: 1.4 ± 0.535
1.167AspArg: 1.167 ± 0.75
4.434AspSer: 4.434 ± 0.541
2.567AspThr: 2.567 ± 0.606
4.667AspVal: 4.667 ± 1.402
1.167AspTrp: 1.167 ± 0.569
3.034AspTyr: 3.034 ± 0.3
0.0AspXaa: 0.0 ± 0.0
Glu
2.8GluAla: 2.8 ± 2.048
0.933GluCys: 0.933 ± 0.701
4.201GluAsp: 4.201 ± 0.969
2.1GluGlu: 2.1 ± 0.772
2.334GluPhe: 2.334 ± 0.729
5.134GluGly: 5.134 ± 1.37
1.4GluHis: 1.4 ± 0.976
3.501GluIle: 3.501 ± 1.147
3.267GluLys: 3.267 ± 0.965
5.601GluLeu: 5.601 ± 1.107
2.334GluMet: 2.334 ± 0.467
1.167GluAsn: 1.167 ± 0.75
1.4GluPro: 1.4 ± 0.562
2.567GluGln: 2.567 ± 0.762
2.8GluArg: 2.8 ± 0.46
3.501GluSer: 3.501 ± 0.671
3.734GluThr: 3.734 ± 1.004
2.8GluVal: 2.8 ± 0.989
1.167GluTrp: 1.167 ± 0.266
3.034GluTyr: 3.034 ± 0.916
0.0GluXaa: 0.0 ± 0.0
Phe
1.867PheAla: 1.867 ± 0.525
0.233PheCys: 0.233 ± 0.311
1.167PheAsp: 1.167 ± 0.838
3.267PheGlu: 3.267 ± 0.967
3.734PhePhe: 3.734 ± 1.727
2.8PheGly: 2.8 ± 0.731
0.467PheHis: 0.467 ± 0.621
3.501PheIle: 3.501 ± 0.84
4.201PheLys: 4.201 ± 1.141
5.368PheLeu: 5.368 ± 2.075
0.0PheMet: 0.0 ± 0.0
2.334PheAsn: 2.334 ± 1.144
3.034PhePro: 3.034 ± 1.13
1.634PheGln: 1.634 ± 1.216
3.034PheArg: 3.034 ± 0.999
3.967PheSer: 3.967 ± 0.607
2.567PheThr: 2.567 ± 1.21
1.4PheVal: 1.4 ± 0.67
0.7PheTrp: 0.7 ± 0.318
0.467PheTyr: 0.467 ± 0.312
0.0PheXaa: 0.0 ± 0.0
Gly
1.4GlyAla: 1.4 ± 0.483
0.0GlyCys: 0.0 ± 0.0
4.201GlyAsp: 4.201 ± 0.746
3.501GlyGlu: 3.501 ± 1.631
2.334GlyPhe: 2.334 ± 0.421
2.567GlyGly: 2.567 ± 0.517
1.634GlyHis: 1.634 ± 0.788
4.201GlyIle: 4.201 ± 0.799
5.834GlyLys: 5.834 ± 1.683
9.568GlyLeu: 9.568 ± 1.296
2.8GlyMet: 2.8 ± 0.521
2.567GlyAsn: 2.567 ± 0.586
3.734GlyPro: 3.734 ± 0.681
1.867GlyGln: 1.867 ± 0.643
3.034GlyArg: 3.034 ± 1.005
3.034GlySer: 3.034 ± 0.616
4.901GlyThr: 4.901 ± 1.679
5.368GlyVal: 5.368 ± 1.067
2.334GlyTrp: 2.334 ± 0.409
2.1GlyTyr: 2.1 ± 0.647
0.0GlyXaa: 0.0 ± 0.0
His
2.1HisAla: 2.1 ± 0.614
0.467HisCys: 0.467 ± 0.312
1.634HisAsp: 1.634 ± 0.814
1.4HisGlu: 1.4 ± 0.667
1.634HisPhe: 1.634 ± 0.888
1.634HisGly: 1.634 ± 0.337
0.467HisHis: 0.467 ± 0.393
1.634HisIle: 1.634 ± 0.528
2.1HisLys: 2.1 ± 0.445
2.334HisLeu: 2.334 ± 0.409
0.933HisMet: 0.933 ± 0.656
2.1HisAsn: 2.1 ± 1.399
1.4HisPro: 1.4 ± 0.321
0.233HisGln: 0.233 ± 0.15
1.4HisArg: 1.4 ± 0.703
2.1HisSer: 2.1 ± 0.55
0.7HisThr: 0.7 ± 0.583
1.867HisVal: 1.867 ± 0.39
0.933HisTrp: 0.933 ± 0.456
1.867HisTyr: 1.867 ± 0.581
0.0HisXaa: 0.0 ± 0.0
Ile
2.8IleAla: 2.8 ± 0.662
1.867IleCys: 1.867 ± 0.522
4.201IleAsp: 4.201 ± 1.237
4.667IleGlu: 4.667 ± 0.719
1.634IlePhe: 1.634 ± 0.578
6.534IleGly: 6.534 ± 1.799
2.1IleHis: 2.1 ± 0.519
2.8IleIle: 2.8 ± 0.828
3.267IleLys: 3.267 ± 0.95
5.368IleLeu: 5.368 ± 0.735
1.167IleMet: 1.167 ± 0.373
3.267IleAsn: 3.267 ± 0.639
3.501IlePro: 3.501 ± 1.977
2.8IleGln: 2.8 ± 0.702
5.834IleArg: 5.834 ± 1.601
4.667IleSer: 4.667 ± 0.841
3.501IleThr: 3.501 ± 0.329
2.8IleVal: 2.8 ± 0.453
0.233IleTrp: 0.233 ± 0.333
2.567IleTyr: 2.567 ± 0.872
0.0IleXaa: 0.0 ± 0.0
Lys
3.967LysAla: 3.967 ± 1.758
1.4LysCys: 1.4 ± 0.597
3.734LysAsp: 3.734 ± 0.856
3.967LysGlu: 3.967 ± 0.492
3.267LysPhe: 3.267 ± 1.1
4.201LysGly: 4.201 ± 1.089
1.4LysHis: 1.4 ± 0.707
4.201LysIle: 4.201 ± 0.892
5.134LysLys: 5.134 ± 1.307
5.601LysLeu: 5.601 ± 0.936
3.267LysMet: 3.267 ± 1.049
3.034LysAsn: 3.034 ± 0.839
0.933LysPro: 0.933 ± 0.499
1.634LysGln: 1.634 ± 0.599
2.567LysArg: 2.567 ± 0.396
6.301LysSer: 6.301 ± 1.272
3.267LysThr: 3.267 ± 0.962
2.334LysVal: 2.334 ± 0.609
2.1LysTrp: 2.1 ± 0.519
2.8LysTyr: 2.8 ± 0.899
0.0LysXaa: 0.0 ± 0.0
Leu
5.601LeuAla: 5.601 ± 0.753
2.567LeuCys: 2.567 ± 0.771
5.134LeuAsp: 5.134 ± 0.797
3.501LeuGlu: 3.501 ± 1.192
4.667LeuPhe: 4.667 ± 0.914
5.601LeuGly: 5.601 ± 0.632
2.567LeuHis: 2.567 ± 1.09
7.468LeuIle: 7.468 ± 2.059
7.001LeuLys: 7.001 ± 0.965
6.534LeuLeu: 6.534 ± 1.81
3.267LeuMet: 3.267 ± 1.307
3.967LeuAsn: 3.967 ± 0.896
4.434LeuPro: 4.434 ± 1.217
2.334LeuGln: 2.334 ± 0.405
5.368LeuArg: 5.368 ± 0.796
7.701LeuSer: 7.701 ± 0.777
5.134LeuThr: 5.134 ± 1.447
3.967LeuVal: 3.967 ± 1.159
0.933LeuTrp: 0.933 ± 0.262
4.901LeuTyr: 4.901 ± 1.235
0.0LeuXaa: 0.0 ± 0.0
Met
1.634MetAla: 1.634 ± 0.449
0.467MetCys: 0.467 ± 0.393
2.1MetAsp: 2.1 ± 0.464
0.933MetGlu: 0.933 ± 0.454
1.167MetPhe: 1.167 ± 0.553
1.4MetGly: 1.4 ± 0.703
0.467MetHis: 0.467 ± 0.3
1.867MetIle: 1.867 ± 0.676
1.4MetLys: 1.4 ± 0.435
2.8MetLeu: 2.8 ± 0.697
0.7MetMet: 0.7 ± 0.568
1.167MetAsn: 1.167 ± 0.37
1.4MetPro: 1.4 ± 0.716
1.167MetGln: 1.167 ± 0.266
0.933MetArg: 0.933 ± 0.6
3.267MetSer: 3.267 ± 1.081
1.634MetThr: 1.634 ± 0.754
2.334MetVal: 2.334 ± 0.517
0.467MetTrp: 0.467 ± 0.3
0.233MetTyr: 0.233 ± 0.333
0.0MetXaa: 0.0 ± 0.0
Asn
1.634AsnAla: 1.634 ± 0.722
0.467AsnCys: 0.467 ± 0.393
1.634AsnAsp: 1.634 ± 0.283
1.867AsnGlu: 1.867 ± 0.646
0.933AsnPhe: 0.933 ± 0.398
3.034AsnGly: 3.034 ± 1.082
1.867AsnHis: 1.867 ± 0.96
2.567AsnIle: 2.567 ± 0.707
0.7AsnLys: 0.7 ± 0.318
3.734AsnLeu: 3.734 ± 1.113
0.0AsnMet: 0.0 ± 0.0
0.933AsnAsn: 0.933 ± 0.6
3.967AsnPro: 3.967 ± 0.806
2.567AsnGln: 2.567 ± 0.712
2.1AsnArg: 2.1 ± 0.533
2.8AsnSer: 2.8 ± 0.493
2.334AsnThr: 2.334 ± 0.689
2.1AsnVal: 2.1 ± 0.321
1.867AsnTrp: 1.867 ± 0.525
3.501AsnTyr: 3.501 ± 0.93
0.0AsnXaa: 0.0 ± 0.0
Pro
2.8ProAla: 2.8 ± 0.625
0.467ProCys: 0.467 ± 0.3
4.434ProAsp: 4.434 ± 1.032
3.734ProGlu: 3.734 ± 1.989
2.334ProPhe: 2.334 ± 1.161
1.4ProGly: 1.4 ± 0.707
2.567ProHis: 2.567 ± 0.891
4.901ProIle: 4.901 ± 1.154
2.334ProLys: 2.334 ± 1.016
4.201ProLeu: 4.201 ± 1.096
0.933ProMet: 0.933 ± 0.625
1.634ProAsn: 1.634 ± 0.449
3.034ProPro: 3.034 ± 0.865
1.167ProGln: 1.167 ± 0.266
0.7ProArg: 0.7 ± 0.45
5.368ProSer: 5.368 ± 1.113
3.034ProThr: 3.034 ± 0.867
2.1ProVal: 2.1 ± 0.536
0.7ProTrp: 0.7 ± 0.283
1.4ProTyr: 1.4 ± 0.672
0.0ProXaa: 0.0 ± 0.0
Gln
1.634GlnAla: 1.634 ± 1.221
1.4GlnCys: 1.4 ± 0.416
1.634GlnAsp: 1.634 ± 0.542
1.867GlnGlu: 1.867 ± 0.384
1.867GlnPhe: 1.867 ± 0.435
3.734GlnGly: 3.734 ± 0.902
0.7GlnHis: 0.7 ± 0.283
2.334GlnIle: 2.334 ± 0.646
1.634GlnLys: 1.634 ± 0.347
1.867GlnLeu: 1.867 ± 0.383
0.933GlnMet: 0.933 ± 0.552
1.4GlnAsn: 1.4 ± 0.46
1.4GlnPro: 1.4 ± 1.448
1.167GlnGln: 1.167 ± 0.362
0.933GlnArg: 0.933 ± 0.478
2.567GlnSer: 2.567 ± 0.615
2.1GlnThr: 2.1 ± 0.55
3.267GlnVal: 3.267 ± 0.623
0.233GlnTrp: 0.233 ± 0.45
1.167GlnTyr: 1.167 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
2.567ArgAla: 2.567 ± 0.521
0.467ArgCys: 0.467 ± 0.3
2.567ArgAsp: 2.567 ± 0.967
3.734ArgGlu: 3.734 ± 0.749
2.1ArgPhe: 2.1 ± 0.519
3.501ArgGly: 3.501 ± 1.491
0.7ArgHis: 0.7 ± 0.283
3.267ArgIle: 3.267 ± 1.007
1.634ArgLys: 1.634 ± 0.846
3.267ArgLeu: 3.267 ± 0.675
1.867ArgMet: 1.867 ± 0.409
2.567ArgAsn: 2.567 ± 1.386
1.867ArgPro: 1.867 ± 1.011
2.1ArgGln: 2.1 ± 0.326
1.4ArgArg: 1.4 ± 0.703
4.201ArgSer: 4.201 ± 1.032
3.034ArgThr: 3.034 ± 0.458
2.8ArgVal: 2.8 ± 0.274
1.167ArgTrp: 1.167 ± 0.306
1.634ArgTyr: 1.634 ± 0.745
0.0ArgXaa: 0.0 ± 0.0
Ser
3.034SerAla: 3.034 ± 0.496
1.867SerCys: 1.867 ± 0.662
5.601SerAsp: 5.601 ± 1.387
3.734SerGlu: 3.734 ± 1.066
3.267SerPhe: 3.267 ± 0.565
4.901SerGly: 4.901 ± 0.451
2.8SerHis: 2.8 ± 0.49
4.901SerIle: 4.901 ± 0.89
4.901SerLys: 4.901 ± 1.362
9.568SerLeu: 9.568 ± 1.286
1.867SerMet: 1.867 ± 0.409
3.967SerAsn: 3.967 ± 1.228
1.867SerPro: 1.867 ± 0.305
3.734SerGln: 3.734 ± 0.7
4.201SerArg: 4.201 ± 0.883
9.335SerSer: 9.335 ± 1.587
4.201SerThr: 4.201 ± 0.909
3.734SerVal: 3.734 ± 0.487
2.1SerTrp: 2.1 ± 0.802
3.267SerTyr: 3.267 ± 0.752
0.0SerXaa: 0.0 ± 0.0
Thr
2.567ThrAla: 2.567 ± 0.512
1.4ThrCys: 1.4 ± 0.404
1.867ThrAsp: 1.867 ± 0.525
1.867ThrGlu: 1.867 ± 0.305
2.8ThrPhe: 2.8 ± 0.714
5.368ThrGly: 5.368 ± 1.402
2.1ThrHis: 2.1 ± 0.52
4.901ThrIle: 4.901 ± 0.701
3.034ThrLys: 3.034 ± 0.794
3.967ThrLeu: 3.967 ± 1.037
1.634ThrMet: 1.634 ± 0.84
1.634ThrAsn: 1.634 ± 0.722
3.501ThrPro: 3.501 ± 1.133
0.7ThrGln: 0.7 ± 0.325
2.334ThrArg: 2.334 ± 0.483
5.601ThrSer: 5.601 ± 1.066
5.134ThrThr: 5.134 ± 1.31
3.734ThrVal: 3.734 ± 0.535
2.334ThrTrp: 2.334 ± 0.862
1.867ThrTyr: 1.867 ± 0.685
0.0ThrXaa: 0.0 ± 0.0
Val
1.4ValAla: 1.4 ± 0.544
0.7ValCys: 0.7 ± 0.45
4.201ValAsp: 4.201 ± 1.457
4.901ValGlu: 4.901 ± 1.085
1.867ValPhe: 1.867 ± 0.534
2.8ValGly: 2.8 ± 1.221
1.867ValHis: 1.867 ± 0.662
3.501ValIle: 3.501 ± 1.35
3.734ValLys: 3.734 ± 0.267
4.667ValLeu: 4.667 ± 0.9
1.167ValMet: 1.167 ± 0.51
2.1ValAsn: 2.1 ± 0.78
3.034ValPro: 3.034 ± 0.496
2.334ValGln: 2.334 ± 0.739
2.8ValArg: 2.8 ± 0.719
5.134ValSer: 5.134 ± 0.886
3.734ValThr: 3.734 ± 0.993
1.867ValVal: 1.867 ± 1.253
0.933ValTrp: 0.933 ± 0.701
1.4ValTyr: 1.4 ± 0.418
0.0ValXaa: 0.0 ± 0.0
Trp
0.7TrpAla: 0.7 ± 0.298
0.0TrpCys: 0.0 ± 0.0
1.634TrpAsp: 1.634 ± 0.64
0.933TrpGlu: 0.933 ± 0.6
1.634TrpPhe: 1.634 ± 0.591
2.1TrpGly: 2.1 ± 0.467
1.167TrpHis: 1.167 ± 0.681
0.933TrpIle: 0.933 ± 0.413
2.1TrpLys: 2.1 ± 0.457
1.867TrpLeu: 1.867 ± 0.583
0.7TrpMet: 0.7 ± 0.298
0.467TrpAsn: 0.467 ± 0.408
0.467TrpPro: 0.467 ± 0.217
0.233TrpGln: 0.233 ± 0.15
0.7TrpArg: 0.7 ± 0.45
2.334TrpSer: 2.334 ± 0.405
0.7TrpThr: 0.7 ± 0.45
1.167TrpVal: 1.167 ± 0.903
0.0TrpTrp: 0.0 ± 0.0
0.467TrpTyr: 0.467 ± 0.393
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.267TyrAla: 3.267 ± 1.267
0.933TyrCys: 0.933 ± 0.786
0.7TyrAsp: 0.7 ± 0.628
2.334TyrGlu: 2.334 ± 0.88
3.034TyrPhe: 3.034 ± 0.284
2.567TyrGly: 2.567 ± 0.586
1.4TyrHis: 1.4 ± 0.375
2.567TyrIle: 2.567 ± 1.184
3.734TyrLys: 3.734 ± 0.912
4.201TyrLeu: 4.201 ± 0.435
1.167TyrMet: 1.167 ± 0.266
1.634TyrAsn: 1.634 ± 0.444
1.867TyrPro: 1.867 ± 0.712
1.167TyrGln: 1.167 ± 0.362
1.4TyrArg: 1.4 ± 0.635
2.1TyrSer: 2.1 ± 0.846
2.334TyrThr: 2.334 ± 0.85
0.933TyrVal: 0.933 ± 0.309
0.233TyrTrp: 0.233 ± 0.45
0.933TyrTyr: 0.933 ± 0.6
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6 proteins (4286 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski