Nocardiopsis flavescens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Nocardiopsis

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6G8V4|A0A1M6G8V4_9ACTN Flp pilus assembly protein CpaB OS=Nocardiopsis flavescens OX=758803 GN=SAMN05421803_103319 PE=4 SV=1
MM1 pKa = 7.15QKK3 pKa = 9.11HH4 pKa = 5.38HH5 pKa = 6.32SRR7 pKa = 11.84RR8 pKa = 11.84TVLGFVAAGALSVPMALSGAVSAGADD34 pKa = 3.69EE35 pKa = 4.94LAPLYY40 pKa = 10.46TSTDD44 pKa = 3.18AVAGEE49 pKa = 4.22WFVVLEE55 pKa = 4.47DD56 pKa = 4.53SISTAAVTPAALGIAAGDD74 pKa = 3.63VNHH77 pKa = 6.98TYY79 pKa = 11.21EE80 pKa = 4.43EE81 pKa = 4.49VFDD84 pKa = 5.12GYY86 pKa = 11.34SATLSTAEE94 pKa = 4.05VQEE97 pKa = 4.7LRR99 pKa = 11.84AQDD102 pKa = 3.16GVAYY106 pKa = 10.07VEE108 pKa = 4.35QVGTAHH114 pKa = 7.04TSVTWGLDD122 pKa = 3.45RR123 pKa = 11.84IDD125 pKa = 5.3QEE127 pKa = 5.42DD128 pKa = 4.22LPLDD132 pKa = 4.04DD133 pKa = 5.66SYY135 pKa = 11.81STTADD140 pKa = 3.2GSGVSAYY147 pKa = 9.8IIDD150 pKa = 3.76TGIDD154 pKa = 3.29PDD156 pKa = 3.96HH157 pKa = 7.48PDD159 pKa = 3.15FEE161 pKa = 5.43GRR163 pKa = 11.84ASSAFDD169 pKa = 3.42AYY171 pKa = 10.67GGGGLDD177 pKa = 3.6VNGHH181 pKa = 4.92GTHH184 pKa = 5.58VAGTIGSATYY194 pKa = 10.31GVAPAADD201 pKa = 3.64LFGVKK206 pKa = 9.7VLSDD210 pKa = 3.99SGSGSYY216 pKa = 11.09DD217 pKa = 3.42DD218 pKa = 5.24VIAGIDD224 pKa = 3.39WVAANAPEE232 pKa = 3.93NSVANLSLGGPASQAVDD249 pKa = 3.56DD250 pKa = 4.95AVNALAGAGVFVAVAAGNEE269 pKa = 4.24GQDD272 pKa = 3.59AGNVSPARR280 pKa = 11.84AEE282 pKa = 4.11GVTTVGASDD291 pKa = 3.37ITDD294 pKa = 3.55TAAYY298 pKa = 9.31FSNHH302 pKa = 4.71GAAVDD307 pKa = 3.93IYY309 pKa = 11.24APGVDD314 pKa = 3.96IEE316 pKa = 4.48STIPGGGVDD325 pKa = 4.74AYY327 pKa = 10.39DD328 pKa = 4.15GTSMASPHH336 pKa = 5.49VAGAAALYY344 pKa = 10.42KK345 pKa = 10.35SANGDD350 pKa = 3.27ADD352 pKa = 3.55QATVQDD358 pKa = 3.68WLVANGSQDD367 pKa = 3.27KK368 pKa = 11.06LSGVPSGTVNTLLNVQGLL386 pKa = 3.81

Molecular weight: 38.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6QJ49|A0A1M6QJ49_9ACTN Pyruvate dehydrogenase (Quinone) OS=Nocardiopsis flavescens OX=758803 GN=SAMN05421803_11586 PE=3 SV=1
MM1 pKa = 7.65PVNALSALALTAVVLLALSGAATQAKK27 pKa = 9.51KK28 pKa = 10.51ARR30 pKa = 11.84KK31 pKa = 8.67AVAAGRR37 pKa = 11.84RR38 pKa = 11.84TATRR42 pKa = 11.84RR43 pKa = 11.84VRR45 pKa = 11.84AALKK49 pKa = 9.42PRR51 pKa = 11.84TVARR55 pKa = 11.84TRR57 pKa = 11.84RR58 pKa = 11.84KK59 pKa = 7.53TPGRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 3.35

Molecular weight: 6.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6204

0

6204

2043562

28

7759

329.4

35.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.908 ± 0.055

0.673 ± 0.009

6.195 ± 0.031

6.214 ± 0.035

2.7 ± 0.017

10.16 ± 0.036

2.272 ± 0.015

2.799 ± 0.024

1.312 ± 0.019

10.446 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.723 ± 0.015

1.546 ± 0.016

6.563 ± 0.036

2.137 ± 0.017

8.71 ± 0.044

4.825 ± 0.02

5.628 ± 0.031

8.82 ± 0.037

1.489 ± 0.014

1.878 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski