Williamwhitmania taraxaci
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3616 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6LB51|A0A1G6LB51_9BACT Chromosome partitioning protein ParB family OS=Williamwhitmania taraxaci OX=1640674 GN=SAMN05216323_103016 PE=3 SV=1
GG1 pKa = 6.63 VTTTDD6 pKa = 3.98 ILPSGYY12 pKa = 9.17 TYY14 pKa = 11.26 VSNDD18 pKa = 2.94 LGASYY23 pKa = 11.37 VPVTGIWTIGNLANGATASLNITATVLATGSYY55 pKa = 10.99 ANTATVTSTTMDD67 pKa = 3.63 PTPGNNTDD75 pKa = 3.36 INTPVPNHH83 pKa = 6.23 IPIAFDD89 pKa = 3.88 DD90 pKa = 4.09 SQTTFIEE97 pKa = 4.65 TPVTIPVLGNDD108 pKa = 3.56 TGLEE112 pKa = 4.13 DD113 pKa = 4.11 GGLAVTITVNPTNGTFVVNGDD134 pKa = 3.32 NTVTYY139 pKa = 9.14 TPNAGFTGTDD149 pKa = 2.98 TFIYY153 pKa = 8.97 QVCDD157 pKa = 3.43 ADD159 pKa = 4.5 GDD161 pKa = 4.48 CATATVTITVNPTAINHH178 pKa = 6.07 LPVAVNDD185 pKa = 4.08 TTSTKK190 pKa = 10.28 QDD192 pKa = 3.4 TPVTIPVLGNDD203 pKa = 3.95 SGLEE207 pKa = 3.99 DD208 pKa = 4.29 GGLMVTITVNPTNGTFVVNGDD229 pKa = 3.32 NTVTYY234 pKa = 9.14 TPNAGFTGIDD244 pKa = 2.68 IFTYY248 pKa = 8.2 QVCDD252 pKa = 3.72 ANGDD256 pKa = 3.96 CTTATVTITVIGTVIVPEE274 pKa = 4.43 GFSPNGDD281 pKa = 2.91 GVNDD285 pKa = 3.55 NFVIPGLDD293 pKa = 3.36 NFDD296 pKa = 4.01 KK297 pKa = 11.19 LSVEE301 pKa = 4.19 IFNRR305 pKa = 11.84 WGNVVYY311 pKa = 10.71 KK312 pKa = 10.4 EE313 pKa = 3.96 EE314 pKa = 4.39 HH315 pKa = 6.63 YY316 pKa = 11.46 KK317 pKa = 10.81 NDD319 pKa = 3.29 WDD321 pKa = 3.82 GKK323 pKa = 8.72 ANVGFTIGSDD333 pKa = 3.88 LPVGTYY339 pKa = 9.71 FFLVTIHH346 pKa = 7.49 DD347 pKa = 4.01 NGKK350 pKa = 10.2 KK351 pKa = 7.69 ITGYY355 pKa = 11.05 VYY357 pKa = 9.38 ITRR360 pKa = 4.25
Molecular weight: 37.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.787
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.478
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.706
Rodwell 3.541
Grimsley 3.376
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.151
Thurlkill 3.541
EMBOSS 3.706
Sillero 3.846
Patrickios 0.299
IPC_peptide 3.719
IPC2_peptide 3.808
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|A0A1G6RK33|A0A1G6RK33_9BACT Uncharacterized protein OS=Williamwhitmania taraxaci OX=1640674 GN=SAMN05216323_107513 PE=4 SV=1
MM1 pKa = 7.51 LGAICTLKK9 pKa = 10.81 CEE11 pKa = 4.07 NVILYY16 pKa = 9.92 SRR18 pKa = 11.84 NSSRR22 pKa = 11.84 TLPRR26 pKa = 11.84 FSTNGIFLLFATVRR40 pKa = 11.84 RR41 pKa = 11.84 VDD43 pKa = 3.53 GFIANSKK50 pKa = 10.06 NKK52 pKa = 10.14
Molecular weight: 5.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.589
IPC_protein 10.189
Toseland 10.672
ProMoST 10.438
Dawson 10.76
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 11.052
Grimsley 10.789
Solomon 10.862
Lehninger 10.847
Nozaki 10.687
DTASelect 10.423
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.687
Patrickios 10.906
IPC_peptide 10.862
IPC2_peptide 9.677
IPC2.peptide.svr19 8.545
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3616
0
3616
1202311
25
3589
332.5
37.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.11 ± 0.044
0.911 ± 0.013
5.037 ± 0.03
6.073 ± 0.044
5.026 ± 0.035
6.785 ± 0.047
1.725 ± 0.018
7.673 ± 0.04
6.827 ± 0.045
9.552 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.45 ± 0.021
5.294 ± 0.041
3.648 ± 0.024
3.315 ± 0.021
4.124 ± 0.028
6.946 ± 0.033
5.825 ± 0.063
6.8 ± 0.036
1.026 ± 0.014
3.852 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here