Hubei virga-like virus 1
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KJV6|A0A1L3KJV6_9VIRU Uncharacterized protein OS=Hubei virga-like virus 1 OX=1923324 PE=4 SV=1
MM1 pKa = 7.69 PKK3 pKa = 10.05 KK4 pKa = 10.01 KK5 pKa = 9.9 RR6 pKa = 11.84 KK7 pKa = 9.4 SRR9 pKa = 11.84 SAPSDD14 pKa = 3.43 EE15 pKa = 4.66 PATSTEE21 pKa = 3.88 VLRR24 pKa = 11.84 EE25 pKa = 3.87 LRR27 pKa = 11.84 SRR29 pKa = 11.84 SVTASLEE36 pKa = 4.39 SEE38 pKa = 4.21 AEE40 pKa = 3.96 TWEE43 pKa = 4.24 SDD45 pKa = 3.54 DD46 pKa = 4.36 TLVSPPPEE54 pKa = 4.04 LVPITRR60 pKa = 11.84 SHH62 pKa = 6.86 PVHH65 pKa = 6.5 AQQTVRR71 pKa = 11.84 PPISTPRR78 pKa = 11.84 PEE80 pKa = 4.42 MTTQPPAPGNAEE92 pKa = 3.67 GHH94 pKa = 5.24 YY95 pKa = 10.38 LGWNDD100 pKa = 4.05 EE101 pKa = 4.24 MEE103 pKa = 4.63 LLSAHH108 pKa = 6.5 SYY110 pKa = 10.79 CSLVDD115 pKa = 3.51 LRR117 pKa = 11.84 NFVHH121 pKa = 7.13 TYY123 pKa = 9.02 MPLAYY128 pKa = 9.5 SVHH131 pKa = 6.22 SNRR134 pKa = 11.84 ARR136 pKa = 11.84 VLEE139 pKa = 3.91 ALKK142 pKa = 10.56 ALRR145 pKa = 11.84 APAPYY150 pKa = 10.16 AEE152 pKa = 4.82 NIRR155 pKa = 11.84 YY156 pKa = 6.29 PQRR159 pKa = 11.84 GFYY162 pKa = 10.63 VDD164 pKa = 4.98 LNSTSISPIISTLMYY179 pKa = 10.78 CCDD182 pKa = 3.68 CTDD185 pKa = 3.35 RR186 pKa = 11.84 ATNVHH191 pKa = 6.09 GQTASQQTQNDD202 pKa = 3.08 VRR204 pKa = 11.84 RR205 pKa = 11.84 SYY207 pKa = 10.71 EE208 pKa = 3.72 VNFAQLQSLAYY219 pKa = 8.67 CAKK222 pKa = 9.96 VDD224 pKa = 3.56 EE225 pKa = 4.56 LARR228 pKa = 11.84 NGIMARR234 pKa = 11.84 AMFEE238 pKa = 4.15 SSNNLTWQQ246 pKa = 3.21
Molecular weight: 27.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.008
IPC2_protein 6.02
IPC_protein 6.109
Toseland 6.415
ProMoST 6.427
Dawson 6.415
Bjellqvist 6.389
Wikipedia 6.44
Rodwell 6.415
Grimsley 6.532
Solomon 6.427
Lehninger 6.427
Nozaki 6.678
DTASelect 6.868
Thurlkill 6.898
EMBOSS 6.868
Sillero 6.81
Patrickios 4.012
IPC_peptide 6.44
IPC2_peptide 6.766
IPC2.peptide.svr19 6.691
Protein with the highest isoelectric point:
>tr|A0A1L3KJV6|A0A1L3KJV6_9VIRU Uncharacterized protein OS=Hubei virga-like virus 1 OX=1923324 PE=4 SV=1
MM1 pKa = 7.31 GHH3 pKa = 6.67 PKK5 pKa = 10.24 ARR7 pKa = 11.84 LYY9 pKa = 10.77 LSKK12 pKa = 10.45 LRR14 pKa = 11.84 KK15 pKa = 9.4 RR16 pKa = 11.84 LGSNPYY22 pKa = 9.31 VRR24 pKa = 11.84 VVLAEE29 pKa = 4.21 LLMLARR35 pKa = 11.84 GDD37 pKa = 3.65 VLLPGVVPVTYY48 pKa = 10.6 GRR50 pKa = 11.84 LHH52 pKa = 6.62 VDD54 pKa = 3.95 RR55 pKa = 11.84 MAEE58 pKa = 3.96 PQLLSDD64 pKa = 4.68 LLAAYY69 pKa = 7.3 MFSRR73 pKa = 11.84 ATIGGDD79 pKa = 3.1 VTEE82 pKa = 4.46 EE83 pKa = 3.95 MVEE86 pKa = 4.6 KK87 pKa = 10.02 MDD89 pKa = 5.93 AIQKK93 pKa = 8.02 FTDD96 pKa = 3.34 LCKK99 pKa = 9.73 TAGIEE104 pKa = 4.06 TAPAMANAAQRR115 pKa = 11.84 VVSRR119 pKa = 11.84 IATTNADD126 pKa = 4.09 LVTGMLRR133 pKa = 11.84 TGSKK137 pKa = 10.54 KK138 pKa = 10.67 LGDD141 pKa = 4.23 RR142 pKa = 11.84 FPISYY147 pKa = 10.31 QLEE150 pKa = 4.07 TSAAARR156 pKa = 11.84 LASEE160 pKa = 4.29 YY161 pKa = 10.46 PEE163 pKa = 4.45 LNVVFGNTPHH173 pKa = 6.43 PHH175 pKa = 6.67 AYY177 pKa = 9.57 AAAATLCQEE186 pKa = 3.91 EE187 pKa = 4.96 LILRR191 pKa = 11.84 RR192 pKa = 11.84 CRR194 pKa = 11.84 YY195 pKa = 9.27 DD196 pKa = 3.28 RR197 pKa = 11.84 KK198 pKa = 9.46 NTVPKK203 pKa = 10.68 DD204 pKa = 3.25 FDD206 pKa = 3.68 AAIVDD211 pKa = 3.68 IGANYY216 pKa = 8.96 HH217 pKa = 5.5 RR218 pKa = 11.84 HH219 pKa = 5.12 AKK221 pKa = 10.1 AGRR224 pKa = 11.84 TTIHH228 pKa = 6.57 CCSPIIEE235 pKa = 4.86 HH236 pKa = 7.04 RR237 pKa = 11.84 DD238 pKa = 3.28 SARR241 pKa = 11.84 DD242 pKa = 3.49 TTRR245 pKa = 11.84 ANQLQHH251 pKa = 6.6 LVSKK255 pKa = 10.81 NIITQRR261 pKa = 11.84 TCNAYY266 pKa = 8.95 TDD268 pKa = 4.31 GKK270 pKa = 11.19 GSLQRR275 pKa = 11.84 CYY277 pKa = 11.28 NLAQDD282 pKa = 4.92 CYY284 pKa = 8.48 VTAPYY289 pKa = 11.14 GMLIHH294 pKa = 6.4 GQYY297 pKa = 10.42 DD298 pKa = 3.23 ISIADD303 pKa = 3.99 LGKK306 pKa = 9.4 MCDD309 pKa = 3.1 AHH311 pKa = 7.93 QFEE314 pKa = 5.03 QVFSVIIFDD323 pKa = 3.43 VGMLFKK329 pKa = 10.85 EE330 pKa = 4.37 SGVVAGVEE338 pKa = 3.8 MNYY341 pKa = 10.06 YY342 pKa = 10.16 RR343 pKa = 11.84 DD344 pKa = 3.64 GSDD347 pKa = 2.98 IVCGYY352 pKa = 10.51 VGDD355 pKa = 4.16 SSVSYY360 pKa = 10.24 RR361 pKa = 11.84 HH362 pKa = 6.22 KK363 pKa = 10.64 LSNIQEE369 pKa = 4.63 IIRR372 pKa = 11.84 TTIMVTPKK380 pKa = 10.4 GRR382 pKa = 11.84 VYY384 pKa = 10.85 YY385 pKa = 10.19 CEE387 pKa = 3.66 KK388 pKa = 7.25 TTRR391 pKa = 11.84 GGAVFLHH398 pKa = 5.46 YY399 pKa = 10.01 VYY401 pKa = 10.64 CRR403 pKa = 11.84 RR404 pKa = 11.84 KK405 pKa = 9.75 PLTATQSVDD414 pKa = 3.6 FSVWYY419 pKa = 8.63 PQDD422 pKa = 3.14 EE423 pKa = 4.69 SAVLLSTFEE432 pKa = 3.93 FDD434 pKa = 3.05 QTVFGDD440 pKa = 3.78 SKK442 pKa = 11.23 VRR444 pKa = 11.84 TVKK447 pKa = 10.9 DD448 pKa = 3.49 PILRR452 pKa = 11.84 FKK454 pKa = 10.94 RR455 pKa = 11.84 RR456 pKa = 11.84 FIEE459 pKa = 3.54 VDD461 pKa = 2.81 KK462 pKa = 11.44 RR463 pKa = 11.84 FFEE466 pKa = 4.63 LLLGHH471 pKa = 7.02 CIRR474 pKa = 11.84 SGDD477 pKa = 3.33 KK478 pKa = 10.78 SFNLNEE484 pKa = 4.44 CFSAANTFNTRR495 pKa = 11.84 MCINGNDD502 pKa = 3.21 VRR504 pKa = 11.84 TVNRR508 pKa = 11.84 VASDD512 pKa = 3.29 PCTRR516 pKa = 11.84 CSICTLQPSGDD527 pKa = 3.86 PRR529 pKa = 11.84 WIAGKK534 pKa = 10.42 GIEE537 pKa = 4.59 AFAKK541 pKa = 10.14 SEE543 pKa = 3.99 KK544 pKa = 9.91 EE545 pKa = 3.6 KK546 pKa = 10.19 RR547 pKa = 11.84 AKK549 pKa = 10.66 GRR551 pKa = 11.84 VTFWNFVEE559 pKa = 4.47 YY560 pKa = 10.11 AWKK563 pKa = 10.33 KK564 pKa = 9.68 SSGPRR569 pKa = 11.84 LLNSIGEE576 pKa = 4.08 IWNDD580 pKa = 4.62 LITDD584 pKa = 3.75 MAEE587 pKa = 3.87 LAEE590 pKa = 4.07 VDD592 pKa = 3.68 QLSAAFFRR600 pKa = 11.84 SVRR603 pKa = 11.84 EE604 pKa = 3.71 VRR606 pKa = 11.84 YY607 pKa = 10.48 SEE609 pKa = 4.06 FLTVSGEE616 pKa = 4.28 YY617 pKa = 9.77 EE618 pKa = 4.16 DD619 pKa = 5.62 HH620 pKa = 7.37 DD621 pKa = 4.74 LPSILMDD628 pKa = 3.44 VKK630 pKa = 11.03 VDD632 pKa = 3.55 SFAMKK637 pKa = 9.87 MEE639 pKa = 4.35 NDD641 pKa = 3.66 HH642 pKa = 6.8 LKK644 pKa = 10.01 EE645 pKa = 3.66 VCDD648 pKa = 3.71 IVRR651 pKa = 11.84 SGLVGEE657 pKa = 4.65 KK658 pKa = 10.49 PEE660 pKa = 4.04 LSKK663 pKa = 11.34 PNVSPKK669 pKa = 10.86 LEE671 pKa = 4.24 GEE673 pKa = 4.22 PVKK676 pKa = 10.35 PATDD680 pKa = 3.37 SKK682 pKa = 11.23 VKK684 pKa = 10.55 NLSQPGTPVAAPKK697 pKa = 10.33 LVSAHH702 pKa = 5.86 NAASEE707 pKa = 4.23 SSQTALTAPSTQQTYY722 pKa = 8.02 LTQEE726 pKa = 4.09 SVVRR730 pKa = 11.84 PVAPGEE736 pKa = 4.12 SRR738 pKa = 11.84 PASSAGSLTMSISVDD753 pKa = 3.44 SAPTMSEE760 pKa = 3.8 EE761 pKa = 3.93 PRR763 pKa = 11.84 PLLSSGSLAMSVSEE777 pKa = 3.85 ISCVNSLPVSDD788 pKa = 3.21 MWDD791 pKa = 3.28 RR792 pKa = 11.84 LSSVGSVSSIPSLCSARR809 pKa = 11.84 SNRR812 pKa = 11.84 TVTRR816 pKa = 11.84 EE817 pKa = 3.94 TEE819 pKa = 4.12 RR820 pKa = 11.84 VRR822 pKa = 11.84 SALVDD827 pKa = 3.61 SVRR830 pKa = 11.84 QDD832 pKa = 3.3 IISIAASEE840 pKa = 4.25 VGSVGSGKK848 pKa = 7.44 QLRR851 pKa = 11.84 MLATKK856 pKa = 10.63 LHH858 pKa = 5.24 MTGTDD863 pKa = 3.09 KK864 pKa = 11.45 SEE866 pKa = 4.01 EE867 pKa = 3.83 NSRR870 pKa = 11.84 RR871 pKa = 11.84 LSKK874 pKa = 10.57 ARR876 pKa = 11.84 KK877 pKa = 8.73 DD878 pKa = 3.56 ALSYY882 pKa = 9.1 TCSGRR887 pKa = 11.84 LTHH890 pKa = 6.75 VPMPADD896 pKa = 3.69 GHH898 pKa = 6.07 CLFHH902 pKa = 7.76 ALAHH906 pKa = 6.12 VIGSTALEE914 pKa = 3.81 VRR916 pKa = 11.84 KK917 pKa = 9.78 QIKK920 pKa = 9.62 AATEE924 pKa = 4.18 PGHH927 pKa = 6.3 DD928 pKa = 3.8 TSHH931 pKa = 7.84 LDD933 pKa = 3.13 IVDD936 pKa = 4.11 GSRR939 pKa = 11.84 AGWGRR944 pKa = 11.84 IEE946 pKa = 4.05 DD947 pKa = 4.16 TIVFSRR953 pKa = 11.84 MTGIRR958 pKa = 11.84 ICTHH962 pKa = 5.36 IVSPHH967 pKa = 5.12 VSFATDD973 pKa = 3.36 AYY975 pKa = 10.72 SAAEE979 pKa = 4.22 LPEE982 pKa = 4.25 TAPVAHH988 pKa = 6.65 LHH990 pKa = 5.26 WQVDD994 pKa = 4.23 GDD996 pKa = 4.37 PMDD999 pKa = 4.3 ASYY1002 pKa = 8.83 TAHH1005 pKa = 7.1 VDD1007 pKa = 3.87 LLVDD1011 pKa = 3.88 NNRR1014 pKa = 11.84 SSDD1017 pKa = 3.42 STLAVVPEE1025 pKa = 4.44 IPLDD1029 pKa = 3.68 SPVNITEE1036 pKa = 3.96 PTAVVTPVTVEE1047 pKa = 3.69 VAEE1050 pKa = 4.45 VVRR1053 pKa = 11.84 EE1054 pKa = 3.95 EE1055 pKa = 4.38 TVSNEE1060 pKa = 3.71 PLVPEE1065 pKa = 4.67 VPDD1068 pKa = 3.36 KK1069 pKa = 11.3 TGIAVTMLPDD1079 pKa = 3.5 EE1080 pKa = 4.67 LKK1082 pKa = 9.99 EE1083 pKa = 3.87 LVGAGVLPWKK1093 pKa = 10.32 EE1094 pKa = 3.2 LFARR1098 pKa = 11.84 VKK1100 pKa = 10.08 KK1101 pKa = 10.54 RR1102 pKa = 11.84 KK1103 pKa = 9.46 NRR1105 pKa = 11.84 DD1106 pKa = 2.96 VFSNLHH1112 pKa = 5.53 RR1113 pKa = 11.84 VGVLPDD1119 pKa = 3.59 RR1120 pKa = 11.84 LAYY1123 pKa = 10.05 EE1124 pKa = 4.6 LDD1126 pKa = 3.77 DD1127 pKa = 5.01 LFATLRR1133 pKa = 11.84 THH1135 pKa = 4.79 TEE1137 pKa = 3.24 EE1138 pKa = 4.63 RR1139 pKa = 11.84 FDD1141 pKa = 3.76 VRR1143 pKa = 11.84 PMTEE1147 pKa = 3.49 KK1148 pKa = 10.79 YY1149 pKa = 10.15 KK1150 pKa = 10.61 RR1151 pKa = 11.84 LCKK1154 pKa = 10.5 YY1155 pKa = 9.07 WDD1157 pKa = 3.32 ASGGDD1162 pKa = 3.53 FVVDD1166 pKa = 4.8 LKK1168 pKa = 11.25 AKK1170 pKa = 9.26 EE1171 pKa = 4.29 YY1172 pKa = 10.57 VWTAPIVYY1180 pKa = 9.69 RR1181 pKa = 11.84 VRR1183 pKa = 11.84 AHH1185 pKa = 5.43 VAEE1188 pKa = 4.69 DD1189 pKa = 3.46 MLLKK1193 pKa = 10.24 RR1194 pKa = 11.84 DD1195 pKa = 3.74 LFVFRR1200 pKa = 11.84 SKK1202 pKa = 9.66 FTAYY1206 pKa = 10.65 NDD1208 pKa = 3.47 DD1209 pKa = 3.26 YY1210 pKa = 11.54 VYY1212 pKa = 11.19 VVNGLQSTGDD1222 pKa = 3.5 PDD1224 pKa = 3.26 AWRR1227 pKa = 11.84 VATVRR1232 pKa = 11.84 SVASAYY1238 pKa = 8.14 TPKK1241 pKa = 10.25 HH1242 pKa = 4.8 VPYY1245 pKa = 10.08 TEE1247 pKa = 4.1 TLIKK1251 pKa = 10.23 EE1252 pKa = 4.16 LMVRR1256 pKa = 11.84 GYY1258 pKa = 10.84 CEE1260 pKa = 4.81 DD1261 pKa = 3.1 VLARR1265 pKa = 11.84 INALVNCKK1273 pKa = 8.71 NHH1275 pKa = 4.92 VVIRR1279 pKa = 11.84 SNCVVCIYY1287 pKa = 11.0 VNEE1290 pKa = 4.23 KK1291 pKa = 10.51 PKK1293 pKa = 10.0 LTTKK1297 pKa = 9.84 MVEE1300 pKa = 4.44 AILKK1304 pKa = 8.99 VIEE1307 pKa = 4.91 PIVPKK1312 pKa = 10.7 NPMRR1316 pKa = 11.84 FFPDD1320 pKa = 2.69 HH1321 pKa = 6.76 KK1322 pKa = 10.28 LAEE1325 pKa = 4.33 KK1326 pKa = 10.37 AVQLEE1331 pKa = 4.4 SKK1333 pKa = 9.27 RR1334 pKa = 11.84 TAGYY1338 pKa = 8.26 STDD1341 pKa = 3.49 GNMAPRR1347 pKa = 11.84 DD1348 pKa = 3.72 EE1349 pKa = 4.48 RR1350 pKa = 11.84 QTGLVTVVRR1359 pKa = 11.84 SDD1361 pKa = 2.94 AYY1363 pKa = 10.06 KK1364 pKa = 10.54 AGKK1367 pKa = 10.17 AMAAPGKK1374 pKa = 9.63 HH1375 pKa = 5.37 QLRR1378 pKa = 11.84 TDD1380 pKa = 3.38 AATVTSTVYY1389 pKa = 10.53 GVEE1392 pKa = 4.52 GTSKK1396 pKa = 10.3 MSFLGDD1402 pKa = 3.05 NRR1404 pKa = 11.84 LRR1406 pKa = 11.84 TIAEE1410 pKa = 4.15 KK1411 pKa = 10.17 PRR1413 pKa = 11.84 EE1414 pKa = 3.99 VAVRR1418 pKa = 11.84 EE1419 pKa = 3.86 ISAVNVVVQPKK1430 pKa = 9.63 RR1431 pKa = 11.84 VMRR1434 pKa = 11.84 SVGCGTDD1441 pKa = 3.03 SQTITVSDD1449 pKa = 4.2 EE1450 pKa = 3.81 FRR1452 pKa = 11.84 KK1453 pKa = 8.64 PACFIRR1459 pKa = 11.84 CAMADD1464 pKa = 3.36 QGTQAPDD1471 pKa = 3.33 AKK1473 pKa = 10.21 PLARR1477 pKa = 11.84 TVDD1480 pKa = 3.53 AVVPTAAGALMKK1492 pKa = 10.26 DD1493 pKa = 3.79 AKK1495 pKa = 10.3 TEE1497 pKa = 4.18 TQVIPAKK1504 pKa = 10.65 SKK1506 pKa = 8.75 LQRR1509 pKa = 11.84 VASLFSVSKK1518 pKa = 10.7 KK1519 pKa = 10.66 SVDD1522 pKa = 3.65 EE1523 pKa = 4.54 NPVDD1527 pKa = 3.24 MMEE1530 pKa = 4.29 IVKK1533 pKa = 10.1 SFPVQKK1539 pKa = 10.7 SADD1542 pKa = 3.65 QLVRR1546 pKa = 11.84 NAWWVSFPHH1555 pKa = 7.16 ALRR1558 pKa = 11.84 THH1560 pKa = 7.24 LYY1562 pKa = 10.56 LCVACQDD1569 pKa = 3.35 GRR1571 pKa = 11.84 LMITKK1576 pKa = 9.36 EE1577 pKa = 3.59 LSKK1580 pKa = 10.81 RR1581 pKa = 11.84 LGVGITVPIKK1591 pKa = 8.14 TLRR1594 pKa = 11.84 TKK1596 pKa = 10.74 GYY1598 pKa = 8.46 TPFTGAISGWWRR1610 pKa = 11.84 TWLTGNVEE1618 pKa = 4.44 HH1619 pKa = 7.06 YY1620 pKa = 10.68 DD1621 pKa = 3.6 VLIVDD1626 pKa = 4.03 NALPLDD1632 pKa = 3.84 TLQTFLSSLPEE1643 pKa = 4.0 DD1644 pKa = 3.93 VCRR1647 pKa = 11.84 VTLDD1651 pKa = 3.49 FAGEE1655 pKa = 3.95 PDD1657 pKa = 5.19 PIRR1660 pKa = 11.84 LIDD1663 pKa = 3.63 VLRR1666 pKa = 11.84 IANRR1670 pKa = 11.84 KK1671 pKa = 8.76 VLLTDD1676 pKa = 3.34 VCSGARR1682 pKa = 11.84 SYY1684 pKa = 11.55 AAVVAVLHH1692 pKa = 5.95 PPKK1695 pKa = 9.9 RR1696 pKa = 11.84 DD1697 pKa = 3.38 PVVEE1701 pKa = 4.14 KK1702 pKa = 10.9 PMVTMPKK1709 pKa = 10.43 LNAEE1713 pKa = 4.4 DD1714 pKa = 3.66 LGKK1717 pKa = 10.61 VRR1719 pKa = 11.84 DD1720 pKa = 4.04 PQLLPGDD1727 pKa = 3.99 SRR1729 pKa = 11.84 EE1730 pKa = 3.82 IAYY1733 pKa = 10.31 RR1734 pKa = 11.84 NAMLEE1739 pKa = 4.28 FKK1741 pKa = 10.55 HH1742 pKa = 5.68 LTANADD1748 pKa = 3.37 NFNAAKK1754 pKa = 9.65 YY1755 pKa = 10.3 KK1756 pKa = 11.21 AMLQEE1761 pKa = 4.59 SKK1763 pKa = 11.08 NGMSSDD1769 pKa = 3.48 VLDD1772 pKa = 3.63 NLRR1775 pKa = 11.84 RR1776 pKa = 11.84 SQMNVYY1782 pKa = 10.39 DD1783 pKa = 3.72 NHH1785 pKa = 6.94 LKK1787 pKa = 10.65 RR1788 pKa = 11.84 FLLTGQSRR1796 pKa = 11.84 TYY1798 pKa = 8.99 SHH1800 pKa = 7.07 GYY1802 pKa = 9.26 AKK1804 pKa = 10.2 EE1805 pKa = 4.28 GYY1807 pKa = 9.44 VAFDD1811 pKa = 3.6 EE1812 pKa = 4.4 KK1813 pKa = 11.02 NQRR1816 pKa = 11.84 FEE1818 pKa = 3.82 TTEE1821 pKa = 4.07 RR1822 pKa = 11.84 YY1823 pKa = 10.24 VITGRR1828 pKa = 11.84 STEE1831 pKa = 3.77 LMLNQRR1837 pKa = 11.84 IAQRR1841 pKa = 11.84 MEE1843 pKa = 3.8 TVPIDD1848 pKa = 3.66 RR1849 pKa = 11.84 VFMPVIEE1856 pKa = 4.38 WTNGPPGCGKK1866 pKa = 9.1 TYY1868 pKa = 10.38 HH1869 pKa = 7.25 IIQNAHH1875 pKa = 6.2 IPISTTQEE1883 pKa = 3.88 PEE1885 pKa = 4.11 DD1886 pKa = 4.35 LVLCMTTEE1894 pKa = 3.94 GRR1896 pKa = 11.84 RR1897 pKa = 11.84 DD1898 pKa = 3.15 VMAKK1902 pKa = 8.88 MKK1904 pKa = 10.42 SKK1906 pKa = 10.25 RR1907 pKa = 11.84 PEE1909 pKa = 3.62 MSDD1912 pKa = 3.12 EE1913 pKa = 3.83 VLQRR1917 pKa = 11.84 DD1918 pKa = 3.87 VRR1920 pKa = 11.84 TVASVLVNGCAVAYY1934 pKa = 9.98 QRR1936 pKa = 11.84 VLLDD1940 pKa = 3.6 EE1941 pKa = 5.26 ALMAHH1946 pKa = 7.42 AGTIGFVVYY1955 pKa = 9.88 YY1956 pKa = 9.88 SQAKK1960 pKa = 8.7 AIAMIGDD1967 pKa = 3.86 IHH1969 pKa = 6.97 QIPYY1973 pKa = 9.87 VDD1975 pKa = 4.13 RR1976 pKa = 11.84 EE1977 pKa = 4.38 HH1978 pKa = 6.23 MCSVLYY1984 pKa = 9.93 HH1985 pKa = 5.62 VPSRR1989 pKa = 11.84 FADD1992 pKa = 3.13 ITKK1995 pKa = 10.41 SLNRR1999 pKa = 11.84 TYY2001 pKa = 10.63 RR2002 pKa = 11.84 CPVDD2006 pKa = 3.3 VTYY2009 pKa = 11.13 ALSAFYY2015 pKa = 10.56 EE2016 pKa = 4.72 GLHH2019 pKa = 4.96 TTNGIILSVRR2029 pKa = 11.84 QLTYY2033 pKa = 10.59 SGNVTHH2039 pKa = 7.49 IDD2041 pKa = 3.55 KK2042 pKa = 11.11 ACTNTLFLVHH2052 pKa = 6.42 LQADD2056 pKa = 3.58 KK2057 pKa = 11.25 DD2058 pKa = 3.96 ALVSEE2063 pKa = 5.57 GYY2065 pKa = 10.68 GKK2067 pKa = 10.75 ASGSAVLTVHH2077 pKa = 6.76 EE2078 pKa = 4.43 AQGLTFNHH2086 pKa = 6.57 VICIRR2091 pKa = 11.84 RR2092 pKa = 11.84 NSKK2095 pKa = 9.74 PLEE2098 pKa = 4.3 IFSSLPYY2105 pKa = 10.27 AIVAISRR2112 pKa = 11.84 HH2113 pKa = 5.45 RR2114 pKa = 11.84 EE2115 pKa = 3.73 SFVYY2119 pKa = 9.31 YY2120 pKa = 10.13 TDD2122 pKa = 4.36 AEE2124 pKa = 4.23 DD2125 pKa = 5.7 AITGLVKK2132 pKa = 10.37 KK2133 pKa = 10.63 AKK2135 pKa = 9.36 TLDD2138 pKa = 3.53 ASSLSVWNRR2147 pKa = 11.84 KK2148 pKa = 8.67 RR2149 pKa = 11.84 YY2150 pKa = 8.78 AANLSAGGVMRR2161 pKa = 11.84 APMTIRR2167 pKa = 11.84 EE2168 pKa = 4.27 DD2169 pKa = 3.43 THH2171 pKa = 6.47 RR2172 pKa = 11.84 TDD2174 pKa = 3.9 EE2175 pKa = 4.16 NLTRR2179 pKa = 11.84 DD2180 pKa = 4.26 LPADD2184 pKa = 3.49 THH2186 pKa = 6.24 NVLVLEE2192 pKa = 4.64 KK2193 pKa = 10.28 DD2194 pKa = 3.34 TGISFALKK2202 pKa = 9.89 EE2203 pKa = 4.15 KK2204 pKa = 10.42 PSLKK2208 pKa = 10.56 GVWEE2212 pKa = 4.13 EE2213 pKa = 4.0 DD2214 pKa = 3.49 LSYY2217 pKa = 11.12 LQLWYY2222 pKa = 10.13 DD2223 pKa = 3.83 DD2224 pKa = 4.63 KK2225 pKa = 11.84 LPGAAIMEE2233 pKa = 4.21 NMGVQEE2239 pKa = 4.0 WMEE2242 pKa = 4.12 TEE2244 pKa = 4.07 DD2245 pKa = 3.78 TFVSLDD2251 pKa = 3.94 SISVNPSLGIMRR2263 pKa = 11.84 KK2264 pKa = 9.61 SGFGKK2269 pKa = 10.06 LRR2271 pKa = 11.84 PRR2273 pKa = 11.84 LRR2275 pKa = 11.84 TFMFPTKK2282 pKa = 10.08 RR2283 pKa = 11.84 PSFKK2287 pKa = 10.42 EE2288 pKa = 3.93 SLLGAVKK2295 pKa = 10.58 RR2296 pKa = 11.84 NLNAPKK2302 pKa = 10.16 LANDD2306 pKa = 4.68 ALDD2309 pKa = 4.06 PDD2311 pKa = 4.96 CIGRR2315 pKa = 11.84 ALFTNFIRR2323 pKa = 11.84 SAIPAEE2329 pKa = 4.0 NLALLDD2335 pKa = 3.96 MLSADD2340 pKa = 3.82 TVSLSSHH2347 pKa = 6.65 LVDD2350 pKa = 4.58 KK2351 pKa = 10.57 WLDD2354 pKa = 3.45 KK2355 pKa = 10.45 QAPAVRR2361 pKa = 11.84 RR2362 pKa = 11.84 QILSEE2367 pKa = 4.28 TPLHH2371 pKa = 5.52 LRR2373 pKa = 11.84 YY2374 pKa = 10.0 YY2375 pKa = 10.93 NSFSYY2380 pKa = 9.56 MIKK2383 pKa = 10.69 GDD2385 pKa = 3.65 VKK2387 pKa = 10.73 PQLEE2391 pKa = 3.94 LSAIQKK2397 pKa = 9.4 YY2398 pKa = 10.01 PSVQTIVYY2406 pKa = 9.61 SDD2408 pKa = 3.6 KK2409 pKa = 11.03 NINAIFCPVFGVLFEE2424 pKa = 4.58 RR2425 pKa = 11.84 LLNLLDD2431 pKa = 3.62 EE2432 pKa = 5.66 RR2433 pKa = 11.84 ILVFTGMSPHH2443 pKa = 6.15 EE2444 pKa = 4.24 FEE2446 pKa = 5.27 KK2447 pKa = 10.95 EE2448 pKa = 3.7 LNARR2452 pKa = 11.84 IAGLDD2457 pKa = 3.37 LSEE2460 pKa = 4.22 VVTVEE2465 pKa = 3.9 EE2466 pKa = 5.48 DD2467 pKa = 2.83 MSKK2470 pKa = 10.86 YY2471 pKa = 10.89 DD2472 pKa = 3.53 KK2473 pKa = 11.33 AQGRR2477 pKa = 11.84 ALRR2480 pKa = 11.84 QFEE2483 pKa = 4.35 DD2484 pKa = 4.32 LLWDD2488 pKa = 4.19 ALGMDD2493 pKa = 4.92 PDD2495 pKa = 4.98 LLRR2498 pKa = 11.84 IWTDD2502 pKa = 2.68 SHH2504 pKa = 6.25 VRR2506 pKa = 11.84 SHH2508 pKa = 6.48 VRR2510 pKa = 11.84 DD2511 pKa = 3.42 RR2512 pKa = 11.84 RR2513 pKa = 11.84 NGIGFDD2519 pKa = 3.35 TEE2521 pKa = 4.29 YY2522 pKa = 10.66 QRR2524 pKa = 11.84 KK2525 pKa = 9.78 SGDD2528 pKa = 3.1 ATTFAGNTLVILAVLLGVYY2547 pKa = 10.18 DD2548 pKa = 3.89 IDD2550 pKa = 6.15 DD2551 pKa = 3.57 IALIMVAGDD2560 pKa = 3.34 DD2561 pKa = 3.69 SYY2563 pKa = 11.97 IFFKK2567 pKa = 10.45 PGCAHH2572 pKa = 7.05 LPDD2575 pKa = 4.55 PSRR2578 pKa = 11.84 RR2579 pKa = 11.84 IADD2582 pKa = 4.36 LFNLEE2587 pKa = 4.52 CKK2589 pKa = 10.25 LLRR2592 pKa = 11.84 NFSVPYY2598 pKa = 9.48 FCSKK2602 pKa = 10.58 FLIQTPQWTYY2612 pKa = 11.37 LVPDD2616 pKa = 4.05 PLKK2619 pKa = 10.63 FVTKK2623 pKa = 10.8 LGRR2626 pKa = 11.84 LDD2628 pKa = 3.3 MANYY2632 pKa = 9.81 KK2633 pKa = 10.13 HH2634 pKa = 6.76 VEE2636 pKa = 4.26 EE2637 pKa = 4.78 YY2638 pKa = 10.34 RR2639 pKa = 11.84 VSCNDD2644 pKa = 2.91 TMSALFNPVVAEE2656 pKa = 4.08 GLSIAVQEE2664 pKa = 4.54 RR2665 pKa = 11.84 YY2666 pKa = 10.45 GGDD2669 pKa = 2.89 IADD2672 pKa = 3.43 ITKK2675 pKa = 10.6 LINILRR2681 pKa = 11.84 SLCSCPAKK2689 pKa = 10.03 FASLFVHH2696 pKa = 7.34 DD2697 pKa = 4.27 YY2698 pKa = 10.65 GVRR2701 pKa = 11.84 LSFDD2705 pKa = 3.41 PSTTKK2710 pKa = 10.9 LNN2712 pKa = 3.64
Molecular weight: 302.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.797
IPC2_protein 7.132
IPC_protein 6.956
Toseland 6.825
ProMoST 7.571
Dawson 7.629
Bjellqvist 8.039
Wikipedia 7.541
Rodwell 7.644
Grimsley 6.927
Solomon 7.629
Lehninger 7.658
Nozaki 8.273
DTASelect 7.834
Thurlkill 7.878
EMBOSS 7.878
Sillero 8.17
Patrickios 4.368
IPC_peptide 7.629
IPC2_peptide 7.234
IPC2.peptide.svr19 7.097
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
2958
246
2712
1479.0
164.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.418 ± 0.426
1.893 ± 0.064
6.22 ± 0.985
5.544 ± 0.624
3.212 ± 0.724
5.003 ± 1.172
2.434 ± 0.188
4.564 ± 0.785
5.206 ± 1.264
9.026 ± 0.41
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.705 ± 0.064
3.685 ± 0.545
5.139 ± 1.367
2.772 ± 0.776
6.761 ± 0.44
7.708 ± 1.307
6.592 ± 0.331
8.824 ± 1.245
0.98 ± 0.109
3.313 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here