Lactobacillus phage Lb

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Heilongjiangvirus; Lactobacillus virus Lb

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2U845|A0A2Z2U845_9CAUD Uncharacterized protein OS=Lactobacillus phage Lb OX=2048517 GN=Lb_41 PE=4 SV=1
MM1 pKa = 7.87PIYY4 pKa = 9.97YY5 pKa = 9.93VKK7 pKa = 10.16PDD9 pKa = 3.91SDD11 pKa = 3.78NKK13 pKa = 10.46FPDD16 pKa = 4.79GITAEE21 pKa = 4.35DD22 pKa = 3.82YY23 pKa = 11.0KK24 pKa = 11.28AITGEE29 pKa = 4.19AYY31 pKa = 10.29VASTTQAPTTQAPTAQATTTSTTNN55 pKa = 2.94

Molecular weight: 5.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z2U825|A0A2Z2U825_9CAUD Uncharacterized protein OS=Lactobacillus phage Lb OX=2048517 GN=Lb_27 PE=4 SV=1
MM1 pKa = 7.71SSNKK5 pKa = 9.47KK6 pKa = 8.31MAATIRR12 pKa = 11.84AAYY15 pKa = 9.7ANYY18 pKa = 10.74GDD20 pKa = 6.1DD21 pKa = 3.81PDD23 pKa = 4.03NWPEE27 pKa = 3.9DD28 pKa = 3.54VKK30 pKa = 11.39KK31 pKa = 10.41EE32 pKa = 3.79IRR34 pKa = 11.84GQTEE38 pKa = 3.84EE39 pKa = 3.79QHH41 pKa = 5.15TAEE44 pKa = 4.18NKK46 pKa = 9.58ILRR49 pKa = 11.84HH50 pKa = 6.24LILHH54 pKa = 7.16GYY56 pKa = 5.47TNKK59 pKa = 10.13YY60 pKa = 8.27VAQEE64 pKa = 3.93RR65 pKa = 11.84SKK67 pKa = 9.57TPQYY71 pKa = 10.13IQQLRR76 pKa = 11.84GRR78 pKa = 11.84MKK80 pKa = 10.6RR81 pKa = 11.84RR82 pKa = 11.84DD83 pKa = 3.78EE84 pKa = 4.51LNYY87 pKa = 10.25QATPDD92 pKa = 3.73EE93 pKa = 4.31LTQLKK98 pKa = 10.89YY99 pKa = 10.66NVKK102 pKa = 10.27HH103 pKa = 5.64MNRR106 pKa = 11.84PNNQGVASVMGRR118 pKa = 11.84DD119 pKa = 3.88KK120 pKa = 11.44DD121 pKa = 3.32WVRR124 pKa = 11.84CMRR127 pKa = 11.84EE128 pKa = 3.83KK129 pKa = 10.79LRR131 pKa = 11.84EE132 pKa = 3.99AANEE136 pKa = 3.88TRR138 pKa = 11.84RR139 pKa = 3.65

Molecular weight: 16.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

12675

37

1317

207.8

23.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.471 ± 0.36

0.544 ± 0.125

6.982 ± 0.343

5.176 ± 0.344

3.471 ± 0.174

7.132 ± 0.631

1.886 ± 0.198

5.704 ± 0.276

7.905 ± 0.579

7.582 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.817 ± 0.218

6.067 ± 0.272

3.172 ± 0.25

4.718 ± 0.224

3.621 ± 0.249

6.895 ± 0.376

7.14 ± 0.405

6.462 ± 0.295

1.341 ± 0.146

3.913 ± 0.36
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski