Amino acid dipepetide frequency for Flavobacterium sp. 123

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.453AlaAla: 4.453 ± 0.109	0.547AlaCys: 0.547 ± 0.033	3.322AlaAsp: 3.322 ± 0.084	3.877AlaGlu: 3.877 ± 0.113	3.356AlaPhe: 3.356 ± 0.069	4.237AlaGly: 4.237 ± 0.097	1.006AlaHis: 1.006 ± 0.037	5.901AlaIle: 5.901 ± 0.099	5.151AlaLys: 5.151 ± 0.086	6.346AlaLeu: 6.346 ± 0.107	1.592AlaMet: 1.592 ± 0.054	3.708AlaAsn: 3.708 ± 0.075	2.06AlaPro: 2.06 ± 0.073	2.468AlaGln: 2.468 ± 0.061	1.821AlaArg: 1.821 ± 0.054	4.498AlaSer: 4.498 ± 0.104	4.401AlaThr: 4.401 ± 0.189	4.27AlaVal: 4.27 ± 0.084	0.571AlaTrp: 0.571 ± 0.023	2.305AlaTyr: 2.305 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.497CysAla: 0.497 ± 0.037	0.108CysCys: 0.108 ± 0.01	0.438CysAsp: 0.438 ± 0.024	0.432CysGlu: 0.432 ± 0.025	0.479CysPhe: 0.479 ± 0.031	0.646CysGly: 0.646 ± 0.036	0.182CysHis: 0.182 ± 0.017	0.639CysIle: 0.639 ± 0.026	0.466CysLys: 0.466 ± 0.023	0.637CysLeu: 0.637 ± 0.028	0.154CysMet: 0.154 ± 0.013	0.447CysAsn: 0.447 ± 0.027	0.372CysPro: 0.372 ± 0.029	0.224CysGln: 0.224 ± 0.017	0.189CysArg: 0.189 ± 0.012	0.675CysSer: 0.675 ± 0.049	0.519CysThr: 0.519 ± 0.033	0.471CysVal: 0.471 ± 0.025	0.064CysTrp: 0.064 ± 0.008	0.328CysTyr: 0.328 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	3.728AspAla: 3.728 ± 0.091	0.455AspCys: 0.455 ± 0.027	2.427AspAsp: 2.427 ± 0.069	3.409AspGlu: 3.409 ± 0.086	3.595AspPhe: 3.595 ± 0.07	3.502AspGly: 3.502 ± 0.109	0.71AspHis: 0.71 ± 0.032	4.174AspIle: 4.174 ± 0.074	4.29AspLys: 4.29 ± 0.072	5.089AspLeu: 5.089 ± 0.099	1.018AspMet: 1.018 ± 0.037	2.976AspAsn: 2.976 ± 0.077	1.546AspPro: 1.546 ± 0.048	1.339AspGln: 1.339 ± 0.037	1.609AspArg: 1.609 ± 0.045	3.095AspSer: 3.095 ± 0.063	2.686AspThr: 2.686 ± 0.061	3.526AspVal: 3.526 ± 0.071	0.666AspTrp: 0.666 ± 0.029	2.62AspTyr: 2.62 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	4.3GluAla: 4.3 ± 0.101	0.349GluCys: 0.349 ± 0.019	3.119GluAsp: 3.119 ± 0.069	4.376GluGlu: 4.376 ± 0.093	2.766GluPhe: 2.766 ± 0.071	3.511GluGly: 3.511 ± 0.068	1.045GluHis: 1.045 ± 0.033	5.851GluIle: 5.851 ± 0.107	6.109GluLys: 6.109 ± 0.123	5.743GluLeu: 5.743 ± 0.098	1.629GluMet: 1.629 ± 0.044	4.769GluAsn: 4.769 ± 0.095	1.463GluPro: 1.463 ± 0.043	2.161GluGln: 2.161 ± 0.054	2.176GluArg: 2.176 ± 0.064	3.329GluSer: 3.329 ± 0.064	3.581GluThr: 3.581 ± 0.069	3.945GluVal: 3.945 ± 0.087	0.57GluTrp: 0.57 ± 0.031	2.25GluTyr: 2.25 ± 0.053	0.0GluXaa: 0.0 ± 0.0
Phe	3.224PheAla: 3.224 ± 0.065	0.443PheCys: 0.443 ± 0.021	3.359PheAsp: 3.359 ± 0.073	3.508PheGlu: 3.508 ± 0.074	2.887PhePhe: 2.887 ± 0.074	3.886PheGly: 3.886 ± 0.081	0.854PheHis: 0.854 ± 0.037	4.09PheIle: 4.09 ± 0.085	3.705PheLys: 3.705 ± 0.082	4.819PheLeu: 4.819 ± 0.11	1.187PheMet: 1.187 ± 0.041	3.15PheAsn: 3.15 ± 0.072	1.811PhePro: 1.811 ± 0.046	1.671PheGln: 1.671 ± 0.045	1.498PheArg: 1.498 ± 0.046	4.188PheSer: 4.188 ± 0.08	2.991PheThr: 2.991 ± 0.067	3.272PheVal: 3.272 ± 0.073	0.567PheTrp: 0.567 ± 0.029	2.155PheTyr: 2.155 ± 0.062	0.0PheXaa: 0.0 ± 0.0
Gly	4.382GlyAla: 4.382 ± 0.18	0.686GlyCys: 0.686 ± 0.041	3.136GlyAsp: 3.136 ± 0.076	3.28GlyGlu: 3.28 ± 0.069	3.693GlyPhe: 3.693 ± 0.085	4.452GlyGly: 4.452 ± 0.105	1.102GlyHis: 1.102 ± 0.041	6.027GlyIle: 6.027 ± 0.101	5.026GlyLys: 5.026 ± 0.086	5.735GlyLeu: 5.735 ± 0.101	1.661GlyMet: 1.661 ± 0.046	3.637GlyAsn: 3.637 ± 0.096	1.356GlyPro: 1.356 ± 0.043	1.891GlyGln: 1.891 ± 0.05	1.886GlyArg: 1.886 ± 0.048	4.193GlySer: 4.193 ± 0.107	4.552GlyThr: 4.552 ± 0.192	4.241GlyVal: 4.241 ± 0.082	0.708GlyTrp: 0.708 ± 0.031	2.654GlyTyr: 2.654 ± 0.066	0.0GlyXaa: 0.0 ± 0.0
His	0.926HisAla: 0.926 ± 0.033	0.167HisCys: 0.167 ± 0.013	0.777HisAsp: 0.777 ± 0.035	0.954HisGlu: 0.954 ± 0.036	1.205HisPhe: 1.205 ± 0.042	0.944HisGly: 0.944 ± 0.032	0.415HisHis: 0.415 ± 0.023	1.369HisIle: 1.369 ± 0.047	1.103HisLys: 1.103 ± 0.037	1.663HisLeu: 1.663 ± 0.055	0.286HisMet: 0.286 ± 0.019	0.928HisAsn: 0.928 ± 0.034	0.846HisPro: 0.846 ± 0.031	0.705HisGln: 0.705 ± 0.029	0.56HisArg: 0.56 ± 0.028	1.024HisSer: 1.024 ± 0.034	0.843HisThr: 0.843 ± 0.032	0.86HisVal: 0.86 ± 0.032	0.206HisTrp: 0.206 ± 0.016	0.761HisTyr: 0.761 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.223IleAla: 6.223 ± 0.09	0.736IleCys: 0.736 ± 0.031	4.861IleAsp: 4.861 ± 0.078	5.64IleGlu: 5.64 ± 0.112	3.76IlePhe: 3.76 ± 0.099	5.55IleGly: 5.55 ± 0.091	1.489IleHis: 1.489 ± 0.043	6.837IleIle: 6.837 ± 0.112	6.173IleLys: 6.173 ± 0.104	7.531IleLeu: 7.531 ± 0.128	1.579IleMet: 1.579 ± 0.046	5.026IleAsn: 5.026 ± 0.074	3.498IlePro: 3.498 ± 0.067	2.918IleGln: 2.918 ± 0.062	2.494IleArg: 2.494 ± 0.062	6.031IleSer: 6.031 ± 0.09	5.199IleThr: 5.199 ± 0.124	5.257IleVal: 5.257 ± 0.093	0.697IleTrp: 0.697 ± 0.031	2.846IleTyr: 2.846 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	5.083LysAla: 5.083 ± 0.095	0.378LysCys: 0.378 ± 0.023	4.095LysAsp: 4.095 ± 0.084	5.949LysGlu: 5.949 ± 0.113	3.081LysPhe: 3.081 ± 0.065	4.638LysGly: 4.638 ± 0.069	1.184LysHis: 1.184 ± 0.042	7.24LysIle: 7.24 ± 0.115	7.199LysLys: 7.199 ± 0.136	6.53LysLeu: 6.53 ± 0.095	2.266LysMet: 2.266 ± 0.057	5.795LysAsn: 5.795 ± 0.104	2.584LysPro: 2.584 ± 0.054	2.678LysGln: 2.678 ± 0.065	2.532LysArg: 2.532 ± 0.062	4.897LysSer: 4.897 ± 0.074	4.93LysThr: 4.93 ± 0.069	4.769LysVal: 4.769 ± 0.08	0.777LysTrp: 0.777 ± 0.033	3.084LysTyr: 3.084 ± 0.065	0.0LysXaa: 0.0 ± 0.0
Leu	6.007LeuAla: 6.007 ± 0.095	0.692LeuCys: 0.692 ± 0.031	4.885LeuAsp: 4.885 ± 0.091	5.892LeuGlu: 5.892 ± 0.1	5.186LeuPhe: 5.186 ± 0.107	5.803LeuGly: 5.803 ± 0.106	1.479LeuHis: 1.479 ± 0.041	7.21LeuIle: 7.21 ± 0.11	7.471LeuLys: 7.471 ± 0.107	8.587LeuLeu: 8.587 ± 0.145	2.067LeuMet: 2.067 ± 0.056	5.624LeuAsn: 5.624 ± 0.075	3.508LeuPro: 3.508 ± 0.067	3.061LeuGln: 3.061 ± 0.074	2.777LeuArg: 2.777 ± 0.067	6.624LeuSer: 6.624 ± 0.093	5.178LeuThr: 5.178 ± 0.12	5.469LeuVal: 5.469 ± 0.095	0.713LeuTrp: 0.713 ± 0.031	3.105LeuTyr: 3.105 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	1.598MetAla: 1.598 ± 0.054	0.152MetCys: 0.152 ± 0.015	1.157MetAsp: 1.157 ± 0.037	1.339MetGlu: 1.339 ± 0.045	0.869MetPhe: 0.869 ± 0.037	1.416MetGly: 1.416 ± 0.046	0.4MetHis: 0.4 ± 0.022	1.786MetIle: 1.786 ± 0.046	2.325MetLys: 2.325 ± 0.052	1.903MetLeu: 1.903 ± 0.048	0.626MetMet: 0.626 ± 0.029	1.44MetAsn: 1.44 ± 0.042	0.833MetPro: 0.833 ± 0.029	0.854MetGln: 0.854 ± 0.031	0.812MetArg: 0.812 ± 0.028	1.463MetSer: 1.463 ± 0.038	1.205MetThr: 1.205 ± 0.042	1.366MetVal: 1.366 ± 0.041	0.157MetTrp: 0.157 ± 0.014	0.697MetTyr: 0.697 ± 0.031	0.0MetXaa: 0.0 ± 0.0
Asn	3.925AsnAla: 3.925 ± 0.072	0.548AsnCys: 0.548 ± 0.063	3.185AsnAsp: 3.185 ± 0.062	3.753AsnGlu: 3.753 ± 0.068	3.174AsnPhe: 3.174 ± 0.07	4.127AsnGly: 4.127 ± 0.138	1.118AsnHis: 1.118 ± 0.038	4.697AsnIle: 4.697 ± 0.084	4.878AsnLys: 4.878 ± 0.088	5.611AsnLeu: 5.611 ± 0.082	1.253AsnMet: 1.253 ± 0.037	3.832AsnAsn: 3.832 ± 0.096	2.912AsnPro: 2.912 ± 0.056	2.46AsnGln: 2.46 ± 0.058	1.998AsnArg: 1.998 ± 0.053	4.153AsnSer: 4.153 ± 0.089	3.754AsnThr: 3.754 ± 0.086	3.637AsnVal: 3.637 ± 0.083	0.776AsnTrp: 0.776 ± 0.036	2.857AsnTyr: 2.857 ± 0.069	0.0AsnXaa: 0.0 ± 0.0
Pro	2.045ProAla: 2.045 ± 0.053	0.197ProCys: 0.197 ± 0.016	1.804ProAsp: 1.804 ± 0.054	2.723ProGlu: 2.723 ± 0.078	2.051ProPhe: 2.051 ± 0.049	1.776ProGly: 1.776 ± 0.058	0.532ProHis: 0.532 ± 0.028	2.997ProIle: 2.997 ± 0.065	2.661ProLys: 2.661 ± 0.066	2.964ProLeu: 2.964 ± 0.065	0.758ProMet: 0.758 ± 0.032	2.35ProAsn: 2.35 ± 0.057	0.7ProPro: 0.7 ± 0.037	1.006ProGln: 1.006 ± 0.035	0.861ProArg: 0.861 ± 0.033	2.222ProSer: 2.222 ± 0.077	2.189ProThr: 2.189 ± 0.079	2.396ProVal: 2.396 ± 0.062	0.305ProTrp: 0.305 ± 0.02	1.352ProTyr: 1.352 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	1.978GlnAla: 1.978 ± 0.05	0.206GlnCys: 0.206 ± 0.021	1.541GlnAsp: 1.541 ± 0.045	2.362GlnGlu: 2.362 ± 0.06	1.656GlnPhe: 1.656 ± 0.043	1.744GlnGly: 1.744 ± 0.101	0.516GlnHis: 0.516 ± 0.029	2.838GlnIle: 2.838 ± 0.055	3.123GlnLys: 3.123 ± 0.068	3.307GlnLeu: 3.307 ± 0.081	0.838GlnMet: 0.838 ± 0.031	2.424GlnAsn: 2.424 ± 0.058	0.958GlnPro: 0.958 ± 0.04	1.243GlnGln: 1.243 ± 0.043	1.008GlnArg: 1.008 ± 0.036	1.991GlnSer: 1.991 ± 0.051	1.983GlnThr: 1.983 ± 0.06	1.927GlnVal: 1.927 ± 0.047	0.37GlnTrp: 0.37 ± 0.023	1.273GlnTyr: 1.273 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	1.99ArgAla: 1.99 ± 0.072	0.189ArgCys: 0.189 ± 0.014	1.58ArgAsp: 1.58 ± 0.048	1.918ArgGlu: 1.918 ± 0.047	1.677ArgPhe: 1.677 ± 0.044	1.712ArgGly: 1.712 ± 0.046	0.497ArgHis: 0.497 ± 0.026	2.779ArgIle: 2.779 ± 0.063	2.62ArgLys: 2.62 ± 0.065	2.895ArgLeu: 2.895 ± 0.073	0.865ArgMet: 0.865 ± 0.027	1.962ArgAsn: 1.962 ± 0.051	0.936ArgPro: 0.936 ± 0.039	0.907ArgGln: 0.907 ± 0.031	1.069ArgArg: 1.069 ± 0.043	1.705ArgSer: 1.705 ± 0.045	1.679ArgThr: 1.679 ± 0.045	1.779ArgVal: 1.779 ± 0.051	0.334ArgTrp: 0.334 ± 0.019	1.293ArgTyr: 1.293 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	3.954SerAla: 3.954 ± 0.105	0.696SerCys: 0.696 ± 0.038	3.523SerAsp: 3.523 ± 0.065	3.89SerGlu: 3.89 ± 0.079	4.082SerPhe: 4.082 ± 0.081	5.045SerGly: 5.045 ± 0.142	1.157SerHis: 1.157 ± 0.043	5.436SerIle: 5.436 ± 0.095	5.131SerLys: 5.131 ± 0.08	5.916SerLeu: 5.916 ± 0.087	1.326SerMet: 1.326 ± 0.039	3.976SerAsn: 3.976 ± 0.086	2.157SerPro: 2.157 ± 0.06	2.251SerGln: 2.251 ± 0.055	1.935SerArg: 1.935 ± 0.05	4.51SerSer: 4.51 ± 0.108	3.697SerThr: 3.697 ± 0.101	4.333SerVal: 4.333 ± 0.091	0.704SerTrp: 0.704 ± 0.036	2.701SerTyr: 2.701 ± 0.067	0.0SerXaa: 0.0 ± 0.0
Thr	4.19ThrAla: 4.19 ± 0.164	0.399ThrCys: 0.399 ± 0.029	3.142ThrAsp: 3.142 ± 0.082	3.359ThrGlu: 3.359 ± 0.078	3.136ThrPhe: 3.136 ± 0.072	4.215ThrGly: 4.215 ± 0.154	0.956ThrHis: 0.956 ± 0.033	5.801ThrIle: 5.801 ± 0.128	4.095ThrLys: 4.095 ± 0.074	5.4ThrLeu: 5.4 ± 0.098	1.006ThrMet: 1.006 ± 0.038	3.738ThrAsn: 3.738 ± 0.122	2.503ThrPro: 2.503 ± 0.074	1.861ThrGln: 1.861 ± 0.053	1.555ThrArg: 1.555 ± 0.067	3.8ThrSer: 3.8 ± 0.109	4.042ThrThr: 4.042 ± 0.172	4.124ThrVal: 4.124 ± 0.153	0.585ThrTrp: 0.585 ± 0.047	2.403ThrTyr: 2.403 ± 0.094	0.0ThrXaa: 0.0 ± 0.0
Val	4.438ValAla: 4.438 ± 0.075	0.563ValCys: 0.563 ± 0.028	3.427ValAsp: 3.427 ± 0.071	3.557ValGlu: 3.557 ± 0.075	3.596ValPhe: 3.596 ± 0.079	3.924ValGly: 3.924 ± 0.095	0.925ValHis: 0.925 ± 0.034	5.067ValIle: 5.067 ± 0.078	4.457ValLys: 4.457 ± 0.069	6.133ValLeu: 6.133 ± 0.088	1.303ValMet: 1.303 ± 0.042	3.606ValAsn: 3.606 ± 0.08	2.164ValPro: 2.164 ± 0.06	1.759ValGln: 1.759 ± 0.05	1.883ValArg: 1.883 ± 0.047	4.596ValSer: 4.596 ± 0.088	4.019ValThr: 4.019 ± 0.167	4.322ValVal: 4.322 ± 0.1	0.588ValTrp: 0.588 ± 0.027	2.383ValTyr: 2.383 ± 0.059	0.0ValXaa: 0.0 ± 0.0
Trp	0.6TrpAla: 0.6 ± 0.028	0.092TrpCys: 0.092 ± 0.01	0.557TrpAsp: 0.557 ± 0.026	0.597TrpGlu: 0.597 ± 0.028	0.53TrpPhe: 0.53 ± 0.025	0.666TrpGly: 0.666 ± 0.029	0.226TrpHis: 0.226 ± 0.015	0.779TrpIle: 0.779 ± 0.031	0.78TrpLys: 0.78 ± 0.03	0.878TrpLeu: 0.878 ± 0.037	0.275TrpMet: 0.275 ± 0.019	0.768TrpAsn: 0.768 ± 0.039	0.217TrpPro: 0.217 ± 0.017	0.365TrpGln: 0.365 ± 0.021	0.329TrpArg: 0.329 ± 0.021	0.609TrpSer: 0.609 ± 0.026	0.538TrpThr: 0.538 ± 0.039	0.586TrpVal: 0.586 ± 0.029	0.11TrpTrp: 0.11 ± 0.011	0.403TrpTyr: 0.403 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.347TyrAla: 2.347 ± 0.063	0.363TyrCys: 0.363 ± 0.021	2.121TyrAsp: 2.121 ± 0.056	2.226TyrGlu: 2.226 ± 0.05	2.486TyrPhe: 2.486 ± 0.053	2.504TyrGly: 2.504 ± 0.06	0.747TyrHis: 0.747 ± 0.029	2.828TyrIle: 2.828 ± 0.055	2.925TyrLys: 2.925 ± 0.07	3.654TyrLeu: 3.654 ± 0.071	0.713TyrMet: 0.713 ± 0.025	2.49TyrAsn: 2.49 ± 0.065	1.463TyrPro: 1.463 ± 0.043	1.465TyrGln: 1.465 ± 0.048	1.396TyrArg: 1.396 ± 0.042	2.818TyrSer: 2.818 ± 0.077	2.323TyrThr: 2.323 ± 0.085	2.16TyrVal: 2.16 ± 0.056	0.439TyrTrp: 0.439 ± 0.023	1.734TyrTyr: 1.734 ± 0.048	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2513 proteins (879023 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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