Macaca mulatta feces associated virus 1
Average proteome isoelectric point is 8.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W5PXG9|A0A1W5PXG9_9VIRU Cap OS=Macaca mulatta feces associated virus 1 OX=2499223 PE=4 SV=1
MM1 pKa = 7.38 SKK3 pKa = 9.87 IYY5 pKa = 10.13 MVTLPRR11 pKa = 11.84 TNTYY15 pKa = 11.39 AEE17 pKa = 4.48 FNALMRR23 pKa = 11.84 YY24 pKa = 7.62 MRR26 pKa = 11.84 QNDD29 pKa = 3.14 VHH31 pKa = 7.39 KK32 pKa = 9.65 WVIGAEE38 pKa = 4.03 QGKK41 pKa = 7.78 TGYY44 pKa = 9.8 DD45 pKa = 2.72 HH46 pKa = 6.32 WQIRR50 pKa = 11.84 LQIGKK55 pKa = 7.13 TWEE58 pKa = 3.79 EE59 pKa = 4.01 VKK61 pKa = 10.62 RR62 pKa = 11.84 DD63 pKa = 4.19 FGAKK67 pKa = 9.58 AHH69 pKa = 5.93 VEE71 pKa = 4.02 EE72 pKa = 6.12 ASDD75 pKa = 2.93 KK76 pKa = 8.59 WTYY79 pKa = 8.12 EE80 pKa = 4.36 CKK82 pKa = 10.48 EE83 pKa = 4.03 GMCWKK88 pKa = 10.67 SSDD91 pKa = 3.21 RR92 pKa = 11.84 SEE94 pKa = 4.01 NRR96 pKa = 11.84 KK97 pKa = 8.73 QRR99 pKa = 11.84 FGKK102 pKa = 10.02 LRR104 pKa = 11.84 YY105 pKa = 7.4 AQKK108 pKa = 10.58 RR109 pKa = 11.84 IIEE112 pKa = 4.26 HH113 pKa = 7.08 LEE115 pKa = 3.9 TTNDD119 pKa = 3.35 RR120 pKa = 11.84 EE121 pKa = 4.38 VVVWYY126 pKa = 10.12 DD127 pKa = 3.29 EE128 pKa = 4.12 EE129 pKa = 5.79 GNAGKK134 pKa = 10.06 SWLTGALWEE143 pKa = 4.68 RR144 pKa = 11.84 GLAYY148 pKa = 10.03 FAVADD153 pKa = 4.1 TSGKK157 pKa = 8.4 TIVMDD162 pKa = 4.01 IASEE166 pKa = 4.03 YY167 pKa = 10.46 LKK169 pKa = 11.03 NGWRR173 pKa = 11.84 PYY175 pKa = 10.68 VIIDD179 pKa = 3.52 IPRR182 pKa = 11.84 AGKK185 pKa = 6.86 WTPEE189 pKa = 3.79 LYY191 pKa = 10.39 EE192 pKa = 4.67 AIEE195 pKa = 4.45 RR196 pKa = 11.84 IKK198 pKa = 11.02 DD199 pKa = 3.65 GLIKK203 pKa = 10.41 DD204 pKa = 3.72 PRR206 pKa = 11.84 YY207 pKa = 10.31 SSEE210 pKa = 3.54 AVNIHH215 pKa = 5.24 GVKK218 pKa = 10.05 ILVNTNTMPKK228 pKa = 9.8 LDD230 pKa = 4.6 KK231 pKa = 10.66 LSKK234 pKa = 10.52 DD235 pKa = 2.58 RR236 pKa = 11.84 WKK238 pKa = 10.62 IITQTEE244 pKa = 4.31 MNNEE248 pKa = 3.92 EE249 pKa = 4.41 QYY251 pKa = 11.3 VDD253 pKa = 3.09 TWGGKK258 pKa = 9.71 AGAFSS263 pKa = 3.46
Molecular weight: 30.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.669
IPC2_protein 7.878
IPC_protein 7.907
Toseland 8.331
ProMoST 8.287
Dawson 8.682
Bjellqvist 8.609
Wikipedia 8.756
Rodwell 8.77
Grimsley 8.361
Solomon 8.873
Lehninger 8.843
Nozaki 8.668
DTASelect 8.536
Thurlkill 8.624
EMBOSS 8.858
Sillero 8.814
Patrickios 4.558
IPC_peptide 8.858
IPC2_peptide 7.351
IPC2.peptide.svr19 7.531
Protein with the highest isoelectric point:
>tr|A0A1W5PXG9|A0A1W5PXG9_9VIRU Cap OS=Macaca mulatta feces associated virus 1 OX=2499223 PE=4 SV=1
MM1 pKa = 8.02 IEE3 pKa = 3.96 FSRR6 pKa = 11.84 RR7 pKa = 11.84 KK8 pKa = 9.55 NNHH11 pKa = 4.95 GNIAHH16 pKa = 6.4 GKK18 pKa = 9.32 DD19 pKa = 3.2 RR20 pKa = 11.84 SITNSGASITIEE32 pKa = 4.1 DD33 pKa = 3.67 SSITSRR39 pKa = 11.84 DD40 pKa = 3.13 SRR42 pKa = 11.84 ISIPEE47 pKa = 3.9 CSVEE51 pKa = 4.24 TGHH54 pKa = 5.75 SHH56 pKa = 6.53 SRR58 pKa = 11.84 TTHH61 pKa = 5.45 EE62 pKa = 3.94 PNINGFSNRR71 pKa = 11.84 IPRR74 pKa = 11.84 SRR76 pKa = 11.84 SEE78 pKa = 3.46 ISGIEE83 pKa = 3.95 STKK86 pKa = 10.8 GSIHH90 pKa = 6.31 SRR92 pKa = 11.84 GGGHH96 pKa = 6.94 RR97 pKa = 11.84 EE98 pKa = 3.42 NDD100 pKa = 3.92 RR101 pKa = 11.84 IKK103 pKa = 10.63 SLHH106 pKa = 6.15 GKK108 pKa = 7.82 TSLGHH113 pKa = 6.09 SLLPTFSISEE123 pKa = 4.04 EE124 pKa = 4.36 TIVHH128 pKa = 6.38 IEE130 pKa = 4.55 LIDD133 pKa = 3.71 GNIIIEE139 pKa = 4.31 LEE141 pKa = 3.9 TRR143 pKa = 11.84 IIDD146 pKa = 3.45 IYY148 pKa = 11.46 DD149 pKa = 3.05 RR150 pKa = 11.84 TLIHH154 pKa = 6.39 NRR156 pKa = 11.84 IGNRR160 pKa = 11.84 GLPGFEE166 pKa = 4.02 PSHH169 pKa = 6.65 EE170 pKa = 4.0 IRR172 pKa = 11.84 HH173 pKa = 5.84 GIIGYY178 pKa = 9.55 SPVKK182 pKa = 10.62 DD183 pKa = 3.18 RR184 pKa = 11.84 VEE186 pKa = 4.46 HH187 pKa = 6.45 ILRR190 pKa = 11.84 GDD192 pKa = 3.23 IPSRR196 pKa = 11.84 KK197 pKa = 7.72 TDD199 pKa = 3.4 LEE201 pKa = 4.54 RR202 pKa = 11.84 ISRR205 pKa = 11.84 KK206 pKa = 9.32 HH207 pKa = 6.25 RR208 pKa = 11.84 STSEE212 pKa = 3.71 SNIAIDD218 pKa = 3.77 EE219 pKa = 4.21 LSILVIHH226 pKa = 7.18 RR227 pKa = 11.84 KK228 pKa = 6.92 TAVHH232 pKa = 6.49 LMDD235 pKa = 4.29 EE236 pKa = 4.36 FRR238 pKa = 11.84 LRR240 pKa = 11.84 SVDD243 pKa = 2.98 TDD245 pKa = 3.37 NSHH248 pKa = 7.05 FAYY251 pKa = 10.62 LSRR254 pKa = 11.84 QIISFRR260 pKa = 11.84 YY261 pKa = 6.92 TDD263 pKa = 3.79 KK264 pKa = 11.29 NHH266 pKa = 7.19 GITFSTCACRR276 pKa = 11.84 SDD278 pKa = 4.3 RR279 pKa = 11.84 RR280 pKa = 11.84 ICVRR284 pKa = 11.84 RR285 pKa = 11.84 TCDD288 pKa = 2.42 RR289 pKa = 11.84 KK290 pKa = 8.49 TFYY293 pKa = 9.78 KK294 pKa = 10.41 TFFRR298 pKa = 11.84 TT299 pKa = 3.37
Molecular weight: 34.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.149
IPC2_protein 8.814
IPC_protein 8.873
Toseland 9.604
ProMoST 9.355
Dawson 9.838
Bjellqvist 9.604
Wikipedia 9.999
Rodwell 10.058
Grimsley 9.897
Solomon 9.897
Lehninger 9.867
Nozaki 9.794
DTASelect 9.531
Thurlkill 9.706
EMBOSS 10.028
Sillero 9.823
Patrickios 7.644
IPC_peptide 9.897
IPC2_peptide 8.478
IPC2.peptide.svr19 7.798
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
562
263
299
281.0
32.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.27 ± 1.643
1.246 ± 0.31
5.694 ± 0.006
7.651 ± 0.698
2.847 ± 0.604
6.94 ± 0.182
4.27 ± 1.512
10.32 ± 2.219
6.406 ± 1.736
5.16 ± 0.104
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.779 ± 0.806
4.626 ± 0.041
2.491 ± 0.134
1.601 ± 0.919
9.253 ± 1.78
8.897 ± 2.767
7.117 ± 0.417
4.093 ± 1.028
2.135 ± 1.55
3.203 ± 1.111
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here