Amino acid dipepetide frequency for Streptomyces kasugaensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.497AlaAla: 23.497 ± 0.177
1.124AlaCys: 1.124 ± 0.023
8.926AlaAsp: 8.926 ± 0.069
8.783AlaGlu: 8.783 ± 0.085
3.553AlaPhe: 3.553 ± 0.047
14.215AlaGly: 14.215 ± 0.106
3.155AlaHis: 3.155 ± 0.043
3.576AlaIle: 3.576 ± 0.032
2.899AlaLys: 2.899 ± 0.06
14.987AlaLeu: 14.987 ± 0.114
2.559AlaMet: 2.559 ± 0.038
1.93AlaAsn: 1.93 ± 0.03
7.521AlaPro: 7.521 ± 0.073
3.888AlaGln: 3.888 ± 0.049
10.706AlaArg: 10.706 ± 0.085
5.419AlaSer: 5.419 ± 0.056
7.229AlaThr: 7.229 ± 0.061
12.331AlaVal: 12.331 ± 0.103
1.824AlaTrp: 1.824 ± 0.031
2.707AlaTyr: 2.707 ± 0.034
0.0AlaXaa: 0.0 ± 0.0
Cys
1.227CysAla: 1.227 ± 0.025
0.104CysCys: 0.104 ± 0.007
0.472CysAsp: 0.472 ± 0.014
0.394CysGlu: 0.394 ± 0.014
0.221CysPhe: 0.221 ± 0.01
0.995CysGly: 0.995 ± 0.023
0.217CysHis: 0.217 ± 0.01
0.206CysIle: 0.206 ± 0.01
0.123CysLys: 0.123 ± 0.007
0.746CysLeu: 0.746 ± 0.017
0.118CysMet: 0.118 ± 0.007
0.127CysAsn: 0.127 ± 0.008
0.512CysPro: 0.512 ± 0.015
0.179CysGln: 0.179 ± 0.009
0.674CysArg: 0.674 ± 0.018
0.442CysSer: 0.442 ± 0.013
0.549CysThr: 0.549 ± 0.016
0.659CysVal: 0.659 ± 0.017
0.129CysTrp: 0.129 ± 0.007
0.153CysTyr: 0.153 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
8.037AspAla: 8.037 ± 0.074
0.413AspCys: 0.413 ± 0.013
3.402AspAsp: 3.402 ± 0.044
3.7AspGlu: 3.7 ± 0.051
1.612AspPhe: 1.612 ± 0.027
6.612AspGly: 6.612 ± 0.072
1.466AspHis: 1.466 ± 0.025
1.963AspIle: 1.963 ± 0.031
1.14AspLys: 1.14 ± 0.029
5.989AspLeu: 5.989 ± 0.051
0.749AspMet: 0.749 ± 0.018
0.884AspAsn: 0.884 ± 0.024
4.413AspPro: 4.413 ± 0.043
1.471AspGln: 1.471 ± 0.026
5.117AspArg: 5.117 ± 0.05
2.422AspSer: 2.422 ± 0.037
3.182AspThr: 3.182 ± 0.044
4.185AspVal: 4.185 ± 0.046
1.04AspTrp: 1.04 ± 0.027
1.093AspTyr: 1.093 ± 0.02
0.0AspXaa: 0.0 ± 0.0
Glu
7.012GluAla: 7.012 ± 0.081
0.383GluCys: 0.383 ± 0.013
2.535GluAsp: 2.535 ± 0.038
3.131GluGlu: 3.131 ± 0.044
1.471GluPhe: 1.471 ± 0.027
3.906GluGly: 3.906 ± 0.044
1.483GluHis: 1.483 ± 0.027
2.27GluIle: 2.27 ± 0.034
1.318GluLys: 1.318 ± 0.03
6.834GluLeu: 6.834 ± 0.067
0.888GluMet: 0.888 ± 0.024
0.947GluAsn: 0.947 ± 0.023
3.212GluPro: 3.212 ± 0.041
2.165GluGln: 2.165 ± 0.036
5.494GluArg: 5.494 ± 0.056
2.495GluSer: 2.495 ± 0.035
2.824GluThr: 2.824 ± 0.035
4.303GluVal: 4.303 ± 0.049
0.759GluTrp: 0.759 ± 0.018
1.052GluTyr: 1.052 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
3.837PheAla: 3.837 ± 0.044
0.274PheCys: 0.274 ± 0.01
1.967PheAsp: 1.967 ± 0.029
1.326PheGlu: 1.326 ± 0.024
0.93PhePhe: 0.93 ± 0.021
3.016PheGly: 3.016 ± 0.032
0.668PheHis: 0.668 ± 0.016
0.802PheIle: 0.802 ± 0.021
0.479PheLys: 0.479 ± 0.014
2.603PheLeu: 2.603 ± 0.037
0.389PheMet: 0.389 ± 0.015
0.542PheAsn: 0.542 ± 0.016
1.471PhePro: 1.471 ± 0.027
0.668PheGln: 0.668 ± 0.018
1.885PheArg: 1.885 ± 0.03
1.433PheSer: 1.433 ± 0.028
2.038PheThr: 2.038 ± 0.037
2.107PheVal: 2.107 ± 0.032
0.404PheTrp: 0.404 ± 0.014
0.536PheTyr: 0.536 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
11.962GlyAla: 11.962 ± 0.103
0.879GlyCys: 0.879 ± 0.02
5.004GlyAsp: 5.004 ± 0.05
5.047GlyGlu: 5.047 ± 0.053
2.862GlyPhe: 2.862 ± 0.033
9.242GlyGly: 9.242 ± 0.091
2.489GlyHis: 2.489 ± 0.035
3.679GlyIle: 3.679 ± 0.043
2.496GlyLys: 2.496 ± 0.045
9.59GlyLeu: 9.59 ± 0.077
2.05GlyMet: 2.05 ± 0.031
1.687GlyAsn: 1.687 ± 0.027
5.568GlyPro: 5.568 ± 0.056
2.783GlyGln: 2.783 ± 0.04
8.113GlyArg: 8.113 ± 0.065
5.135GlySer: 5.135 ± 0.055
6.54GlyThr: 6.54 ± 0.056
7.319GlyVal: 7.319 ± 0.068
1.675GlyTrp: 1.675 ± 0.031
2.235GlyTyr: 2.235 ± 0.034
0.0GlyXaa: 0.0 ± 0.0
His
2.759HisAla: 2.759 ± 0.039
0.244HisCys: 0.244 ± 0.01
1.396HisAsp: 1.396 ± 0.026
1.193HisGlu: 1.193 ± 0.023
0.683HisPhe: 0.683 ± 0.016
2.535HisGly: 2.535 ± 0.034
0.779HisHis: 0.779 ± 0.019
0.738HisIle: 0.738 ± 0.017
0.376HisLys: 0.376 ± 0.014
2.583HisLeu: 2.583 ± 0.032
0.33HisMet: 0.33 ± 0.013
0.373HisAsn: 0.373 ± 0.014
1.962HisPro: 1.962 ± 0.027
0.722HisGln: 0.722 ± 0.018
2.307HisArg: 2.307 ± 0.036
1.128HisSer: 1.128 ± 0.024
1.496HisThr: 1.496 ± 0.025
1.585HisVal: 1.585 ± 0.028
0.386HisTrp: 0.386 ± 0.014
0.496HisTyr: 0.496 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
5.06IleAla: 5.06 ± 0.05
0.29IleCys: 0.29 ± 0.011
2.232IleAsp: 2.232 ± 0.034
2.023IleGlu: 2.023 ± 0.034
0.728IlePhe: 0.728 ± 0.017
3.788IleGly: 3.788 ± 0.045
0.632IleHis: 0.632 ± 0.019
0.905IleIle: 0.905 ± 0.025
0.751IleLys: 0.751 ± 0.019
2.446IleLeu: 2.446 ± 0.033
0.438IleMet: 0.438 ± 0.015
0.667IleAsn: 0.667 ± 0.021
1.919IlePro: 1.919 ± 0.026
0.716IleGln: 0.716 ± 0.018
2.37IleArg: 2.37 ± 0.032
1.762IleSer: 1.762 ± 0.03
2.216IleThr: 2.216 ± 0.035
2.77IleVal: 2.77 ± 0.037
0.389IleTrp: 0.389 ± 0.013
0.533IleTyr: 0.533 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
2.731LysAla: 2.731 ± 0.048
0.114LysCys: 0.114 ± 0.008
1.339LysAsp: 1.339 ± 0.033
1.159LysGlu: 1.159 ± 0.026
0.471LysPhe: 0.471 ± 0.014
1.773LysGly: 1.773 ± 0.038
0.413LysHis: 0.413 ± 0.014
0.871LysIle: 0.871 ± 0.021
0.865LysLys: 0.865 ± 0.033
1.932LysLeu: 1.932 ± 0.033
0.407LysMet: 0.407 ± 0.015
0.537LysAsn: 0.537 ± 0.019
1.269LysPro: 1.269 ± 0.026
0.679LysGln: 0.679 ± 0.019
1.411LysArg: 1.411 ± 0.03
1.197LysSer: 1.197 ± 0.029
1.292LysThr: 1.292 ± 0.028
1.743LysVal: 1.743 ± 0.03
0.256LysTrp: 0.256 ± 0.012
0.433LysTyr: 0.433 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
15.855LeuAla: 15.855 ± 0.135
0.914LeuCys: 0.914 ± 0.021
6.753LeuAsp: 6.753 ± 0.053
4.285LeuGlu: 4.285 ± 0.049
2.664LeuPhe: 2.664 ± 0.038
9.345LeuGly: 9.345 ± 0.085
2.469LeuHis: 2.469 ± 0.036
3.427LeuIle: 3.427 ± 0.046
1.882LeuLys: 1.882 ± 0.035
11.422LeuLeu: 11.422 ± 0.111
1.606LeuMet: 1.606 ± 0.031
1.631LeuAsn: 1.631 ± 0.03
6.743LeuPro: 6.743 ± 0.059
2.101LeuGln: 2.101 ± 0.032
9.021LeuArg: 9.021 ± 0.069
5.235LeuSer: 5.235 ± 0.051
7.089LeuThr: 7.089 ± 0.058
8.379LeuVal: 8.379 ± 0.071
1.273LeuTrp: 1.273 ± 0.027
1.791LeuTyr: 1.791 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
2.37MetAla: 2.37 ± 0.034
0.136MetCys: 0.136 ± 0.008
0.869MetAsp: 0.869 ± 0.021
0.679MetGlu: 0.679 ± 0.018
0.431MetPhe: 0.431 ± 0.014
1.321MetGly: 1.321 ± 0.025
0.343MetHis: 0.343 ± 0.013
0.64MetIle: 0.64 ± 0.017
0.399MetLys: 0.399 ± 0.014
1.629MetLeu: 1.629 ± 0.029
0.306MetMet: 0.306 ± 0.013
0.436MetAsn: 0.436 ± 0.013
1.104MetPro: 1.104 ± 0.024
0.455MetGln: 0.455 ± 0.015
1.394MetArg: 1.394 ± 0.025
1.311MetSer: 1.311 ± 0.025
1.467MetThr: 1.467 ± 0.028
1.28MetVal: 1.28 ± 0.025
0.215MetTrp: 0.215 ± 0.011
0.345MetTyr: 0.345 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.157AsnAla: 2.157 ± 0.033
0.159AsnCys: 0.159 ± 0.008
0.918AsnAsp: 0.918 ± 0.02
0.75AsnGlu: 0.75 ± 0.016
0.493AsnPhe: 0.493 ± 0.016
1.86AsnGly: 1.86 ± 0.033
0.375AsnHis: 0.375 ± 0.013
0.659AsnIle: 0.659 ± 0.016
0.387AsnLys: 0.387 ± 0.014
1.581AsnLeu: 1.581 ± 0.027
0.274AsnMet: 0.274 ± 0.011
0.392AsnAsn: 0.392 ± 0.013
1.28AsnPro: 1.28 ± 0.025
0.502AsnGln: 0.502 ± 0.017
1.25AsnArg: 1.25 ± 0.027
0.885AsnSer: 0.885 ± 0.022
1.105AsnThr: 1.105 ± 0.023
1.249AsnVal: 1.249 ± 0.026
0.288AsnTrp: 0.288 ± 0.012
0.409AsnTyr: 0.409 ± 0.013
0.0AsnXaa: 0.0 ± 0.0
Pro
9.393ProAla: 9.393 ± 0.072
0.385ProCys: 0.385 ± 0.013
4.568ProAsp: 4.568 ± 0.055
4.171ProGlu: 4.171 ± 0.05
1.57ProPhe: 1.57 ± 0.028
6.928ProGly: 6.928 ± 0.061
1.489ProHis: 1.489 ± 0.025
1.258ProIle: 1.258 ± 0.024
1.157ProLys: 1.157 ± 0.024
5.423ProLeu: 5.423 ± 0.06
0.959ProMet: 0.959 ± 0.021
0.877ProAsn: 0.877 ± 0.021
3.52ProPro: 3.52 ± 0.053
1.909ProGln: 1.909 ± 0.039
4.21ProArg: 4.21 ± 0.049
2.974ProSer: 2.974 ± 0.04
3.072ProThr: 3.072 ± 0.045
5.538ProVal: 5.538 ± 0.053
0.927ProTrp: 0.927 ± 0.023
1.401ProTyr: 1.401 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.727GlnAla: 3.727 ± 0.054
0.176GlnCys: 0.176 ± 0.008
1.473GlnAsp: 1.473 ± 0.027
1.481GlnGlu: 1.481 ± 0.029
0.697GlnPhe: 0.697 ± 0.018
2.265GlnGly: 2.265 ± 0.034
0.733GlnHis: 0.733 ± 0.022
1.095GlnIle: 1.095 ± 0.022
0.588GlnLys: 0.588 ± 0.018
3.3GlnLeu: 3.3 ± 0.039
0.477GlnMet: 0.477 ± 0.016
0.504GlnAsn: 0.504 ± 0.021
1.731GlnPro: 1.731 ± 0.029
1.396GlnGln: 1.396 ± 0.036
2.488GlnArg: 2.488 ± 0.033
1.225GlnSer: 1.225 ± 0.024
1.323GlnThr: 1.323 ± 0.026
2.306GlnVal: 2.306 ± 0.031
0.469GlnTrp: 0.469 ± 0.015
0.543GlnTyr: 0.543 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
10.326ArgAla: 10.326 ± 0.082
0.668ArgCys: 0.668 ± 0.017
4.378ArgAsp: 4.378 ± 0.049
4.731ArgGlu: 4.731 ± 0.047
2.308ArgPhe: 2.308 ± 0.033
6.049ArgGly: 6.049 ± 0.051
2.268ArgHis: 2.268 ± 0.035
3.5ArgIle: 3.5 ± 0.044
1.682ArgLys: 1.682 ± 0.032
8.905ArgLeu: 8.905 ± 0.066
1.772ArgMet: 1.772 ± 0.03
1.364ArgAsn: 1.364 ± 0.025
5.294ArgPro: 5.294 ± 0.06
2.483ArgGln: 2.483 ± 0.033
8.065ArgArg: 8.065 ± 0.079
3.995ArgSer: 3.995 ± 0.041
5.793ArgThr: 5.793 ± 0.056
5.544ArgVal: 5.544 ± 0.056
1.403ArgTrp: 1.403 ± 0.025
1.867ArgTyr: 1.867 ± 0.03
0.0ArgXaa: 0.0 ± 0.0
Ser
6.662SerAla: 6.662 ± 0.065
0.431SerCys: 0.431 ± 0.013
2.436SerAsp: 2.436 ± 0.038
2.247SerGlu: 2.247 ± 0.033
1.494SerPhe: 1.494 ± 0.028
5.905SerGly: 5.905 ± 0.053
1.028SerHis: 1.028 ± 0.022
1.345SerIle: 1.345 ± 0.026
0.954SerLys: 0.954 ± 0.023
4.735SerLeu: 4.735 ± 0.048
0.997SerMet: 0.997 ± 0.024
0.818SerAsn: 0.818 ± 0.02
3.108SerPro: 3.108 ± 0.041
1.216SerGln: 1.216 ± 0.023
3.586SerArg: 3.586 ± 0.041
2.568SerSer: 2.568 ± 0.041
2.747SerThr: 2.747 ± 0.041
4.054SerVal: 4.054 ± 0.042
0.896SerTrp: 0.896 ± 0.02
1.211SerTyr: 1.211 ± 0.025
0.0SerXaa: 0.0 ± 0.0
Thr
9.516ThrAla: 9.516 ± 0.075
0.419ThrCys: 0.419 ± 0.013
3.577ThrAsp: 3.577 ± 0.048
3.231ThrGlu: 3.231 ± 0.037
1.631ThrPhe: 1.631 ± 0.028
7.053ThrGly: 7.053 ± 0.068
1.247ThrHis: 1.247 ± 0.029
1.675ThrIle: 1.675 ± 0.027
1.121ThrLys: 1.121 ± 0.026
5.724ThrLeu: 5.724 ± 0.055
0.939ThrMet: 0.939 ± 0.02
0.914ThrAsn: 0.914 ± 0.021
4.054ThrPro: 4.054 ± 0.048
1.261ThrGln: 1.261 ± 0.029
3.909ThrArg: 3.909 ± 0.045
2.948ThrSer: 2.948 ± 0.041
3.736ThrThr: 3.736 ± 0.054
6.085ThrVal: 6.085 ± 0.056
0.877ThrTrp: 0.877 ± 0.019
1.302ThrTyr: 1.302 ± 0.023
0.0ThrXaa: 0.0 ± 0.0
Val
10.357ValAla: 10.357 ± 0.08
0.789ValCys: 0.789 ± 0.018
4.482ValAsp: 4.482 ± 0.044
4.289ValGlu: 4.289 ± 0.049
2.409ValPhe: 2.409 ± 0.032
6.208ValGly: 6.208 ± 0.059
2.011ValHis: 2.011 ± 0.028
3.002ValIle: 3.002 ± 0.04
1.608ValLys: 1.608 ± 0.031
9.429ValLeu: 9.429 ± 0.088
1.358ValMet: 1.358 ± 0.026
1.594ValAsn: 1.594 ± 0.028
5.121ValPro: 5.121 ± 0.055
2.126ValGln: 2.126 ± 0.032
7.135ValArg: 7.135 ± 0.067
4.026ValSer: 4.026 ± 0.046
5.405ValThr: 5.405 ± 0.059
7.169ValVal: 7.169 ± 0.072
1.109ValTrp: 1.109 ± 0.024
1.473ValTyr: 1.473 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.577TrpAla: 1.577 ± 0.027
0.167TrpCys: 0.167 ± 0.009
0.798TrpAsp: 0.798 ± 0.021
0.711TrpGlu: 0.711 ± 0.017
0.506TrpPhe: 0.506 ± 0.015
1.065TrpGly: 1.065 ± 0.024
0.41TrpHis: 0.41 ± 0.013
0.554TrpIle: 0.554 ± 0.015
0.321TrpLys: 0.321 ± 0.013
1.831TrpLeu: 1.831 ± 0.034
0.28TrpMet: 0.28 ± 0.01
0.368TrpAsn: 0.368 ± 0.014
0.818TrpPro: 0.818 ± 0.018
0.69TrpGln: 0.69 ± 0.019
1.36TrpArg: 1.36 ± 0.025
0.956TrpSer: 0.956 ± 0.021
0.968TrpThr: 0.968 ± 0.016
0.952TrpVal: 0.952 ± 0.022
0.329TrpTrp: 0.329 ± 0.012
0.343TrpTyr: 0.343 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.766TyrAla: 2.766 ± 0.04
0.18TyrCys: 0.18 ± 0.01
1.364TyrAsp: 1.364 ± 0.03
1.149TyrGlu: 1.149 ± 0.024
0.639TyrPhe: 0.639 ± 0.019
2.277TyrGly: 2.277 ± 0.034
0.399TyrHis: 0.399 ± 0.013
0.469TyrIle: 0.469 ± 0.015
0.346TyrLys: 0.346 ± 0.012
2.196TyrLeu: 2.196 ± 0.03
0.237TyrMet: 0.237 ± 0.011
0.38TyrAsn: 0.38 ± 0.014
1.106TyrPro: 1.106 ± 0.025
0.587TyrGln: 0.587 ± 0.018
1.899TyrArg: 1.899 ± 0.029
0.882TyrSer: 0.882 ± 0.019
1.146TyrThr: 1.146 ± 0.024
1.544TyrVal: 1.544 ± 0.027
0.357TyrTrp: 0.357 ± 0.014
0.425TyrTyr: 0.425 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7310 proteins (2333771 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski