Capybara microvirus Cap3_SP_410
Average proteome isoelectric point is 5.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4R3|A0A4P8W4R3_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_410 OX=2585446 PE=4 SV=1
MM1 pKa = 7.68 AKK3 pKa = 10.58 LNIFGFNQDD12 pKa = 3.8 AVPLEE17 pKa = 4.46 DD18 pKa = 5.21 DD19 pKa = 4.28 KK20 pKa = 11.8 LPHH23 pKa = 7.09 RR24 pKa = 11.84 ISQVFFDD31 pKa = 4.06 EE32 pKa = 4.59 EE33 pKa = 3.88 QDD35 pKa = 3.28 KK36 pKa = 10.86 FIPFKK41 pKa = 10.85 DD42 pKa = 3.32 NPRR45 pKa = 11.84 FVSASAVDD53 pKa = 4.39 LPSLLAAGIQPGEE66 pKa = 3.88 AHH68 pKa = 6.11 QEE70 pKa = 3.62 ISSLSFVADD79 pKa = 4.55 FNRR82 pKa = 11.84 SLSSFPQIDD91 pKa = 3.55 NQTSNKK97 pKa = 9.59 SS98 pKa = 3.19
Molecular weight: 10.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.738
IPC2_protein 4.609
IPC_protein 4.482
Toseland 4.304
ProMoST 4.622
Dawson 4.457
Bjellqvist 4.609
Wikipedia 4.38
Rodwell 4.317
Grimsley 4.215
Solomon 4.457
Lehninger 4.406
Nozaki 4.571
DTASelect 4.8
Thurlkill 4.342
EMBOSS 4.393
Sillero 4.609
Patrickios 4.38
IPC_peptide 4.457
IPC2_peptide 4.584
IPC2.peptide.svr19 4.584
Protein with the highest isoelectric point:
>tr|A0A4P8W4Y2|A0A4P8W4Y2_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_410 OX=2585446 PE=4 SV=1
MM1 pKa = 7.41 KK2 pKa = 10.36 VLNNSCYY9 pKa = 10.04 FSPVNYY15 pKa = 9.64 RR16 pKa = 11.84 KK17 pKa = 10.56 YY18 pKa = 11.11 NFLPYY23 pKa = 10.12 SSNYY27 pKa = 10.48 SYY29 pKa = 11.5 DD30 pKa = 3.4 LSKK33 pKa = 11.06 SLSYY37 pKa = 10.72 LVRR40 pKa = 11.84 CYY42 pKa = 9.98 YY43 pKa = 7.96 QWKK46 pKa = 10.41 YY47 pKa = 11.03 SVEE50 pKa = 4.18 LCNGHH55 pKa = 6.65 CFLFMLTYY63 pKa = 10.78 NDD65 pKa = 3.49 NNLPLFDD72 pKa = 5.97 DD73 pKa = 5.26 FFPCHH78 pKa = 6.19 SSKK81 pKa = 10.93 DD82 pKa = 3.17 IRR84 pKa = 11.84 DD85 pKa = 3.42 FTKK88 pKa = 10.56 RR89 pKa = 11.84 LNYY92 pKa = 9.6 YY93 pKa = 9.35 IKK95 pKa = 10.59 SKK97 pKa = 10.46 SASFEE102 pKa = 3.5 WSYY105 pKa = 11.5 FCSKK109 pKa = 10.68 EE110 pKa = 3.9 FGEE113 pKa = 4.43 KK114 pKa = 8.9 TKK116 pKa = 10.64 RR117 pKa = 11.84 PHH119 pKa = 4.79 YY120 pKa = 10.43 HH121 pKa = 6.44 FLLFTDD127 pKa = 5.11 FNEE130 pKa = 4.56 SVDD133 pKa = 4.17 EE134 pKa = 4.06 ICRR137 pKa = 11.84 LVNLAWITRR146 pKa = 11.84 DD147 pKa = 3.14 GSKK150 pKa = 10.91 GGFVSVSPKK159 pKa = 10.13 HH160 pKa = 5.0 GAEE163 pKa = 3.71 IKK165 pKa = 10.91 SFGALKK171 pKa = 10.49 YY172 pKa = 9.66 VIKK175 pKa = 10.79 YY176 pKa = 8.85 VIKK179 pKa = 9.66 DD180 pKa = 3.29 TSTRR184 pKa = 11.84 KK185 pKa = 10.36 YY186 pKa = 10.5 YY187 pKa = 10.85 DD188 pKa = 5.04 DD189 pKa = 4.6 IYY191 pKa = 10.85 QHH193 pKa = 7.94 IYY195 pKa = 9.36 MNNEE199 pKa = 3.36 TSFDD203 pKa = 3.48 VDD205 pKa = 4.1 LDD207 pKa = 3.72 MQIYY211 pKa = 9.92 YY212 pKa = 10.69 YY213 pKa = 11.13 NNHH216 pKa = 6.44 CNNNNIVDD224 pKa = 3.71 NGTIDD229 pKa = 3.9 FLCEE233 pKa = 3.38 ISKK236 pKa = 11.05 SKK238 pKa = 10.57 GFQKK242 pKa = 10.47 FRR244 pKa = 11.84 DD245 pKa = 3.93 AYY247 pKa = 9.64 ISSKK251 pKa = 9.58 TDD253 pKa = 2.61 SDD255 pKa = 3.51 FRR257 pKa = 11.84 QWKK260 pKa = 8.92 NEE262 pKa = 4.12 CGVKK266 pKa = 9.63 PQYY269 pKa = 10.71 SKK271 pKa = 11.4 RR272 pKa = 11.84 LGNFALQHH280 pKa = 6.09 ISNNTIKK287 pKa = 10.6 IPDD290 pKa = 3.74 GEE292 pKa = 4.15 NFKK295 pKa = 10.78 FVNIPLYY302 pKa = 10.46 LYY304 pKa = 10.36 RR305 pKa = 11.84 KK306 pKa = 9.83 LYY308 pKa = 10.51 YY309 pKa = 10.64 NYY311 pKa = 10.28 FQDD314 pKa = 3.81 GEE316 pKa = 4.59 KK317 pKa = 10.36 NVHH320 pKa = 5.74 YY321 pKa = 9.75 FLNEE325 pKa = 3.55 HH326 pKa = 6.04 GVALKK331 pKa = 10.29 YY332 pKa = 10.62 KK333 pKa = 9.88 QFNSHH338 pKa = 6.14 ICNDD342 pKa = 2.84 VCFNYY347 pKa = 10.74 DD348 pKa = 4.43 LIRR351 pKa = 11.84 SSEE354 pKa = 3.82 YY355 pKa = 11.38 SNILKK360 pKa = 10.59 GDD362 pKa = 4.05 FSQSYY367 pKa = 10.57 DD368 pKa = 3.59 FIYY371 pKa = 10.85 RR372 pKa = 11.84 LTLYY376 pKa = 10.68 NSLYY380 pKa = 10.31 RR381 pKa = 11.84 DD382 pKa = 3.81 RR383 pKa = 11.84 FCPNLEE389 pKa = 5.11 DD390 pKa = 4.02 YY391 pKa = 11.41 VCDD394 pKa = 3.92 IDD396 pKa = 4.68 PKK398 pKa = 11.09 KK399 pKa = 10.99 DD400 pKa = 3.94 FIKK403 pKa = 10.88 FNTHH407 pKa = 7.32 AIFHH411 pKa = 6.27 PEE413 pKa = 3.68 CHH415 pKa = 6.75 DD416 pKa = 3.79 FSSLSSSQKK425 pKa = 10.61 YY426 pKa = 9.85 NLITNFYY433 pKa = 6.92 QHH435 pKa = 7.09 KK436 pKa = 8.92 FSTYY440 pKa = 8.48 NNHH443 pKa = 7.02 PYY445 pKa = 10.15 FEE447 pKa = 4.49 EE448 pKa = 4.11 VSKK451 pKa = 11.25 YY452 pKa = 11.29 SNLLYY457 pKa = 10.74 DD458 pKa = 4.18 LTYY461 pKa = 10.58 QYY463 pKa = 11.58 YY464 pKa = 10.76 FNLFKK469 pKa = 10.76 NAQQSYY475 pKa = 9.42 DD476 pKa = 3.35 FVRR479 pKa = 11.84 RR480 pKa = 11.84 TRR482 pKa = 11.84 QQYY485 pKa = 9.84 YY486 pKa = 10.75 GEE488 pKa = 4.27 TT489 pKa = 3.59
Molecular weight: 58.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.126
IPC2_protein 7.41
IPC_protein 7.234
Toseland 6.942
ProMoST 7.79
Dawson 7.922
Bjellqvist 8.287
Wikipedia 7.849
Rodwell 7.936
Grimsley 7.015
Solomon 7.951
Lehninger 7.966
Nozaki 8.361
DTASelect 8.053
Thurlkill 8.083
EMBOSS 8.126
Sillero 8.39
Patrickios 0.973
IPC_peptide 7.951
IPC2_peptide 7.38
IPC2.peptide.svr19 7.401
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1575
98
617
393.8
45.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.46 ± 2.056
1.778 ± 0.649
6.476 ± 0.501
4.762 ± 1.016
6.349 ± 1.199
4.635 ± 0.668
2.286 ± 0.47
5.397 ± 0.602
5.714 ± 0.912
8.19 ± 0.342
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.587 ± 0.342
8.127 ± 1.161
3.302 ± 0.753
5.524 ± 0.81
3.937 ± 0.09
9.016 ± 0.778
4.063 ± 0.696
5.397 ± 0.741
1.079 ± 0.182
6.921 ± 1.873
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here