Rubrobacter sp. SCSIO 52909

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Rubrobacteria; Rubrobacterales; Rubrobacteraceae; Rubrobacter; unclassified Rubrobacter

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4356 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8Q5H0|A0A6G8Q5H0_9ACTN Uncharacterized protein OS=Rubrobacter sp. SCSIO 52909 OX=2653851 GN=GBA63_03050 PE=4 SV=1
MM1 pKa = 6.59QQKK4 pKa = 9.7QEE6 pKa = 4.06TTIVNITDD14 pKa = 3.31NAAEE18 pKa = 4.19KK19 pKa = 10.71VKK21 pKa = 11.06ALMAQEE27 pKa = 4.51GEE29 pKa = 3.94EE30 pKa = 4.14DD31 pKa = 3.22LALRR35 pKa = 11.84IGVRR39 pKa = 11.84PGGCSGFQYY48 pKa = 10.87NIYY51 pKa = 10.3FDD53 pKa = 5.9DD54 pKa = 5.34EE55 pKa = 4.22IADD58 pKa = 3.93DD59 pKa = 4.87DD60 pKa = 4.71EE61 pKa = 4.87VFEE64 pKa = 4.46TKK66 pKa = 9.93GVKK69 pKa = 9.65LAIDD73 pKa = 3.98AMSVPYY79 pKa = 10.68IMGSEE84 pKa = 4.13FDD86 pKa = 4.04YY87 pKa = 11.62VDD89 pKa = 3.48GLMGAGFAVNNPNVQGGCGCGSSFTCC115 pKa = 5.34

Molecular weight: 12.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8QFK8|A0A6G8QFK8_9ACTN Complex1_49kDa domain-containing protein OS=Rubrobacter sp. SCSIO 52909 OX=2653851 GN=GBA63_18905 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.21QPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.78RR12 pKa = 11.84AKK14 pKa = 8.45THH16 pKa = 5.35GFRR19 pKa = 11.84KK20 pKa = 9.92RR21 pKa = 11.84MSSVGGRR28 pKa = 11.84RR29 pKa = 11.84VIAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LAVV44 pKa = 3.5

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4356

0

4356

1316622

37

2091

302.3

32.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.248 ± 0.048

0.716 ± 0.011

5.578 ± 0.032

7.672 ± 0.047

3.554 ± 0.024

9.996 ± 0.038

1.825 ± 0.019

3.83 ± 0.028

2.669 ± 0.026

10.209 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.039 ± 0.015

2.304 ± 0.021

5.42 ± 0.027

2.329 ± 0.019

8.028 ± 0.045

5.306 ± 0.024

4.987 ± 0.026

8.534 ± 0.035

1.27 ± 0.014

2.486 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski