Thermosipho africanus (strain TCF52B)

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Thermotogales; Fervidobacteriaceae; Thermosipho; Thermosipho africanus

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1918 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7IFS0|B7IFS0_THEAB Uncharacterized protein OS=Thermosipho africanus (strain TCF52B) OX=484019 GN=THA_443 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.35KK3 pKa = 10.35FLVLLVSLVVLGVVFADD20 pKa = 3.55VTFSGGVDD28 pKa = 2.94VSYY31 pKa = 9.72TLEE34 pKa = 4.13SSPLKK39 pKa = 10.64ISDD42 pKa = 4.03SVSLSWDD49 pKa = 3.93RR50 pKa = 11.84IEE52 pKa = 4.28MTASGDD58 pKa = 3.46GYY60 pKa = 8.74YY61 pKa = 10.74LRR63 pKa = 11.84VIYY66 pKa = 10.35DD67 pKa = 3.44SSNSFAIDD75 pKa = 3.23QAYY78 pKa = 6.94MTFSFDD84 pKa = 3.52PVTLYY89 pKa = 10.71VGKK92 pKa = 10.26KK93 pKa = 10.14GYY95 pKa = 8.8WLNPYY100 pKa = 10.56AFTDD104 pKa = 4.34GNSLTWNYY112 pKa = 11.18SGVSWYY118 pKa = 10.22YY119 pKa = 8.82PKK121 pKa = 10.44EE122 pKa = 3.79YY123 pKa = 10.58LGAKK127 pKa = 9.75VSFDD131 pKa = 3.32NGSVYY136 pKa = 7.95MTLDD140 pKa = 3.06PRR142 pKa = 11.84YY143 pKa = 9.63AEE145 pKa = 4.94GSATPSVNVFGNVSFDD161 pKa = 3.67PVSLGFIAQDD171 pKa = 3.27NFKK174 pKa = 10.68EE175 pKa = 4.11LGLGVTVNVAPVTASAAFGYY195 pKa = 10.19SIEE198 pKa = 4.33NSTITALLVGVNGTFDD214 pKa = 3.67PVSVLGEE221 pKa = 3.86VDD223 pKa = 3.46LTDD226 pKa = 4.33FNAIVVNGIVNYY238 pKa = 9.02TINDD242 pKa = 3.44KK243 pKa = 10.42MDD245 pKa = 3.91AGLNAKK251 pKa = 9.98YY252 pKa = 10.78NLGDD256 pKa = 3.93DD257 pKa = 3.83KK258 pKa = 11.88SFGTSAWFNYY268 pKa = 9.17YY269 pKa = 10.95YY270 pKa = 10.7NDD272 pKa = 4.43NITLTPSVSFDD283 pKa = 3.62GEE285 pKa = 4.57VITLNMNAHH294 pKa = 6.81FDD296 pKa = 3.7FF297 pKa = 5.46

Molecular weight: 32.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7IE45|B7IE45_THEAB Glutamine synthetase type I OS=Thermosipho africanus (strain TCF52B) OX=484019 GN=glnA2 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.91QPSRR9 pKa = 11.84IKK11 pKa = 10.52RR12 pKa = 11.84KK13 pKa = 8.24RR14 pKa = 11.84THH16 pKa = 6.4GFLARR21 pKa = 11.84KK22 pKa = 7.64KK23 pKa = 7.76TAGGRR28 pKa = 11.84KK29 pKa = 7.66VLKK32 pKa = 9.64NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.77GRR39 pKa = 11.84WRR41 pKa = 11.84LAVV44 pKa = 3.35

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1918

0

1918

595921

37

1649

310.7

35.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.121 ± 0.052

0.573 ± 0.018

5.12 ± 0.043

7.851 ± 0.081

5.692 ± 0.061

6.08 ± 0.051

1.313 ± 0.021

9.771 ± 0.065

9.6 ± 0.066

9.639 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.121 ± 0.023

5.591 ± 0.048

3.271 ± 0.03

2.043 ± 0.024

3.486 ± 0.038

6.136 ± 0.046

4.413 ± 0.036

6.928 ± 0.049

0.836 ± 0.02

4.415 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski