Paenibacillus popilliae ATCC 14706

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; Paenibacillus popilliae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M9LP25|M9LP25_PAEPP Transcriptional regulator/sugar kinase OS=Paenibacillus popilliae ATCC 14706 OX=1212764 GN=PPOP_1523 PE=4 SV=1
MM1 pKa = 7.56INVTDD6 pKa = 3.94KK7 pKa = 10.65QLEE10 pKa = 4.45IIKK13 pKa = 10.09EE14 pKa = 3.87LAPDD18 pKa = 3.85VEE20 pKa = 4.84QYY22 pKa = 10.37IASQDD27 pKa = 3.51PNDD30 pKa = 4.77FYY32 pKa = 11.26TALDD36 pKa = 3.8DD37 pKa = 6.25AIIDD41 pKa = 4.12YY42 pKa = 10.94YY43 pKa = 11.39DD44 pKa = 4.54SNQEE48 pKa = 3.56WLTPNGQKK56 pKa = 9.93LQKK59 pKa = 10.22LYY61 pKa = 11.23DD62 pKa = 4.75DD63 pKa = 4.68IYY65 pKa = 10.84SANEE69 pKa = 3.43II70 pKa = 4.13

Molecular weight: 8.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M9LG19|M9LG19_PAEPP cAMP-binding protein OS=Paenibacillus popilliae ATCC 14706 OX=1212764 GN=PPOP_0750 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 8.7PTFKK6 pKa = 10.81PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.06NGRR28 pKa = 11.84NVIAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3844

0

3844

989251

20

3022

257.3

28.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.481 ± 0.051

0.904 ± 0.015

5.222 ± 0.032

7.111 ± 0.05

3.851 ± 0.032

7.235 ± 0.04

2.304 ± 0.022

6.52 ± 0.044

5.344 ± 0.046

9.668 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.022 ± 0.022

3.609 ± 0.033

4.063 ± 0.026

4.082 ± 0.031

5.72 ± 0.043

5.8 ± 0.033

5.291 ± 0.034

7.195 ± 0.03

1.215 ± 0.017

3.362 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski