Oceanobacillus oncorhynchi
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4386 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A1MLP3|A0A0A1MLP3_9BACI Cystathionine gamma-synthase/O-acetylhomoserine (Thiol)-lyase OS=Oceanobacillus oncorhynchi OX=545501 GN=metI PE=3 SV=1
MM1 pKa = 7.18 LKK3 pKa = 9.95 KK4 pKa = 9.75 WFFFIATLVFVSALYY19 pKa = 10.47 ACSGGDD25 pKa = 4.17 DD26 pKa = 3.8 GDD28 pKa = 4.2 SKK30 pKa = 11.1 EE31 pKa = 4.22 GEE33 pKa = 4.08 AAGGDD38 pKa = 3.74 NVNATGFPIVEE49 pKa = 4.49 EE50 pKa = 4.05 EE51 pKa = 4.42 LEE53 pKa = 4.1 LTIFANKK60 pKa = 8.56 PAQNEE65 pKa = 4.15 GNDD68 pKa = 3.57 WNDD71 pKa = 2.75 ILIWNTYY78 pKa = 10.16 RR79 pKa = 11.84 DD80 pKa = 3.75 LTNINVNWNSVSPDD94 pKa = 3.09 ALEE97 pKa = 4.15 EE98 pKa = 3.98 NRR100 pKa = 11.84 NLALGSGDD108 pKa = 4.09 LPDD111 pKa = 4.49 AFFLAEE117 pKa = 4.79 LSNTDD122 pKa = 3.68 LLRR125 pKa = 11.84 YY126 pKa = 9.34 GSQGAFLPLNDD137 pKa = 5.84 LIDD140 pKa = 4.31 EE141 pKa = 4.35 YY142 pKa = 11.74 APNLKK147 pKa = 10.42 ALMEE151 pKa = 4.59 NDD153 pKa = 3.42 PTIEE157 pKa = 4.04 KK158 pKa = 10.55 AITFPDD164 pKa = 3.68 GNIYY168 pKa = 10.87 SMPSLIEE175 pKa = 4.77 DD176 pKa = 4.05 DD177 pKa = 4.36 FLSLRR182 pKa = 11.84 LSARR186 pKa = 11.84 PWVNEE191 pKa = 3.52 DD192 pKa = 3.02 WLNEE196 pKa = 3.75 LDD198 pKa = 3.64 MDD200 pKa = 4.28 IPEE203 pKa = 4.45 TTGDD207 pKa = 3.31 FYY209 pKa = 11.51 EE210 pKa = 4.13 YY211 pKa = 10.74 LKK213 pKa = 10.83 AVKK216 pKa = 10.14 EE217 pKa = 3.99 LDD219 pKa = 3.43 PVGNGDD225 pKa = 4.24 TIPYY229 pKa = 9.4 GGTDD233 pKa = 2.94 IAEE236 pKa = 4.22 LVQWLSGSFGVMNHH250 pKa = 6.26 GASNTNLDD258 pKa = 4.69 LDD260 pKa = 4.75 PNDD263 pKa = 3.95 DD264 pKa = 3.8 SKK266 pKa = 11.74 VRR268 pKa = 11.84 YY269 pKa = 8.93 YY270 pKa = 10.46 ATADD274 pKa = 3.45 EE275 pKa = 4.16 YY276 pKa = 11.48 RR277 pKa = 11.84 EE278 pKa = 3.92 MLEE281 pKa = 4.31 YY282 pKa = 10.42 IHH284 pKa = 6.45 MLYY287 pKa = 10.67 EE288 pKa = 4.35 EE289 pKa = 4.44 GLIDD293 pKa = 4.2 QSIFTIEE300 pKa = 3.65 WGQFLANASDD310 pKa = 3.62 NLYY313 pKa = 11.17 GSMIFYY319 pKa = 10.71 DD320 pKa = 4.5 PIEE323 pKa = 4.34 LFGEE327 pKa = 4.68 EE328 pKa = 4.82 IGSQYY333 pKa = 11.39 NSLAALEE340 pKa = 5.1 GPDD343 pKa = 3.69 GHH345 pKa = 6.63 QSYY348 pKa = 11.15 NKK350 pKa = 9.86 LSSSVWTPDD359 pKa = 2.93 NLVITSEE366 pKa = 4.25 NPNPAATVRR375 pKa = 11.84 WMDD378 pKa = 3.36 HH379 pKa = 6.28 FYY381 pKa = 11.23 GDD383 pKa = 3.91 EE384 pKa = 4.15 GAEE387 pKa = 3.99 LYY389 pKa = 11.42 YY390 pKa = 10.25 MGVEE394 pKa = 4.09 GEE396 pKa = 4.28 TFEE399 pKa = 5.76 IEE401 pKa = 4.17 DD402 pKa = 4.0 GEE404 pKa = 4.55 AVYY407 pKa = 10.79 SDD409 pKa = 5.26 HH410 pKa = 7.0 ILNPEE415 pKa = 3.49 GDD417 pKa = 3.72 MTFEE421 pKa = 3.74 QAIAQDD427 pKa = 4.27 LTWLGSISGIIKK439 pKa = 10.05 ADD441 pKa = 3.49 YY442 pKa = 9.7 FQGGEE447 pKa = 4.01 TAPQSMEE454 pKa = 3.67 AAEE457 pKa = 4.56 KK458 pKa = 10.47 IEE460 pKa = 3.91 PQVIDD465 pKa = 3.93 EE466 pKa = 4.07 VWPRR470 pKa = 11.84 FTFTEE475 pKa = 4.12 EE476 pKa = 3.75 EE477 pKa = 3.93 NRR479 pKa = 11.84 ILQSTGEE486 pKa = 4.68 DD487 pKa = 2.72 INKK490 pKa = 9.52 YY491 pKa = 8.28 VEE493 pKa = 3.8 EE494 pKa = 4.26 MRR496 pKa = 11.84 DD497 pKa = 3.28 KK498 pKa = 11.1 FITGDD503 pKa = 3.6 EE504 pKa = 4.73 DD505 pKa = 4.33 LDD507 pKa = 3.67 NWDD510 pKa = 4.82 SYY512 pKa = 11.15 IQTIEE517 pKa = 3.82 QMGMEE522 pKa = 4.66 EE523 pKa = 4.08 LQEE526 pKa = 4.38 VYY528 pKa = 9.93 QAAYY532 pKa = 10.44 DD533 pKa = 4.14 RR534 pKa = 11.84 YY535 pKa = 10.37 QSNN538 pKa = 3.03
Molecular weight: 60.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.541
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.617
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.668
Nozaki 3.821
DTASelect 4.024
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.859
Patrickios 1.43
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|A0A0A1M526|A0A0A1M526_9BACI HTH-type transcriptional regulator CynR OS=Oceanobacillus oncorhynchi OX=545501 GN=cynR_2 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 8.12 KK14 pKa = 8.46 VHH16 pKa = 5.5 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MSTKK25 pKa = 9.9 NGRR28 pKa = 11.84 HH29 pKa = 4.47 ILARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.09 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.522
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.735
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.457
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4386
0
4386
1268742
29
2686
289.3
32.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.418 ± 0.038
0.647 ± 0.011
5.326 ± 0.033
7.952 ± 0.053
4.547 ± 0.033
6.835 ± 0.039
2.069 ± 0.022
8.107 ± 0.038
6.373 ± 0.042
9.561 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.88 ± 0.019
4.344 ± 0.027
3.516 ± 0.019
3.95 ± 0.03
3.821 ± 0.027
5.917 ± 0.026
5.382 ± 0.023
6.786 ± 0.031
1.026 ± 0.016
3.542 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here