Garlic virus D
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V5NX44|V5NX44_9VIRU RNA-directed RNA polymerase OS=Garlic virus D OX=12430 PE=4 SV=1
MM1 pKa = 7.07 GTVQKK6 pKa = 10.9 LLDD9 pKa = 4.13 QISDD13 pKa = 3.73 PNARR17 pKa = 11.84 AAYY20 pKa = 10.16 SQDD23 pKa = 3.38 CLSAAKK29 pKa = 10.04 AAATKK34 pKa = 10.98 AMTIAPYY41 pKa = 10.46 AVNQTEE47 pKa = 4.18 ALTLEE52 pKa = 4.81 RR53 pKa = 11.84 IGITTSPFATTSHH66 pKa = 4.89 THH68 pKa = 5.93 AADD71 pKa = 4.1 KK72 pKa = 10.63 IIEE75 pKa = 4.28 NDD77 pKa = 3.51 CLDD80 pKa = 3.67 IIGRR84 pKa = 11.84 YY85 pKa = 8.46 LPKK88 pKa = 10.68 EE89 pKa = 4.03 PVTLIQLKK97 pKa = 9.75 RR98 pKa = 11.84 AKK100 pKa = 10.22 LHH102 pKa = 5.63 LLRR105 pKa = 11.84 RR106 pKa = 11.84 GPSGDD111 pKa = 2.8 IFKK114 pKa = 10.65 NYY116 pKa = 9.13 CHH118 pKa = 6.56 EE119 pKa = 4.62 PKK121 pKa = 10.43 DD122 pKa = 3.62 VLRR125 pKa = 11.84 FGITNPHH132 pKa = 5.96 VCPQVNTTCAVISDD146 pKa = 4.21 TLHH149 pKa = 6.6 FMSTQQVWTLFQNNPSLEE167 pKa = 4.11 RR168 pKa = 11.84 LYY170 pKa = 10.37 GTLVLPIEE178 pKa = 4.47 ALHH181 pKa = 6.34 RR182 pKa = 11.84 LPSLFPEE189 pKa = 4.68 IYY191 pKa = 10.22 KK192 pKa = 10.54 LEE194 pKa = 4.4 YY195 pKa = 9.26 YY196 pKa = 9.99 DD197 pKa = 4.11 KK198 pKa = 10.55 HH199 pKa = 5.63 FAYY202 pKa = 9.87 IPGRR206 pKa = 11.84 HH207 pKa = 5.8 GGGAYY212 pKa = 8.62 VHH214 pKa = 6.94 SYY216 pKa = 8.51 GTLKK220 pKa = 10.22 WLSTAQIGPGPYY232 pKa = 9.24 PEE234 pKa = 4.37 YY235 pKa = 10.33 RR236 pKa = 11.84 SNANGRR242 pKa = 11.84 RR243 pKa = 11.84 PLLDD247 pKa = 3.52 HH248 pKa = 6.95 LSVEE252 pKa = 4.38 KK253 pKa = 10.75 IEE255 pKa = 4.43 TKK257 pKa = 10.11 AAHH260 pKa = 6.64 HH261 pKa = 6.39 IVLIQRR267 pKa = 11.84 RR268 pKa = 11.84 RR269 pKa = 11.84 PEE271 pKa = 4.56 LPWPLPPGWVYY282 pKa = 10.57 HH283 pKa = 6.34 ASDD286 pKa = 4.03 YY287 pKa = 11.61 VKK289 pKa = 10.48 LPEE292 pKa = 3.88 IFYY295 pKa = 9.44 PAEE298 pKa = 4.09 ANVQKK303 pKa = 10.47 AYY305 pKa = 9.92 PHH307 pKa = 6.42 IFIKK311 pKa = 10.52 RR312 pKa = 11.84 LQLYY316 pKa = 8.31 CFSVKK321 pKa = 10.2 AVSLRR326 pKa = 11.84 DD327 pKa = 3.18 IFAKK331 pKa = 9.72 IRR333 pKa = 11.84 QVIPTEE339 pKa = 3.68 EE340 pKa = 4.04 LARR343 pKa = 11.84 FSTADD348 pKa = 4.67 LIRR351 pKa = 11.84 LANYY355 pKa = 10.44 LLFITGMQSTSDD367 pKa = 3.71 YY368 pKa = 10.66 QSPLMEE374 pKa = 4.71 SLLGKK379 pKa = 10.15 LCLSIRR385 pKa = 11.84 ARR387 pKa = 11.84 VRR389 pKa = 11.84 EE390 pKa = 4.06 FFQNILGKK398 pKa = 10.11 SSYY401 pKa = 10.85 AALLTVTDD409 pKa = 3.35 VRR411 pKa = 11.84 PIYY414 pKa = 9.46 FTTTPRR420 pKa = 11.84 RR421 pKa = 11.84 RR422 pKa = 11.84 KK423 pKa = 9.28 ICGEE427 pKa = 3.59 AWFDD431 pKa = 3.59 SLEE434 pKa = 4.24 EE435 pKa = 4.29 EE436 pKa = 4.74 EE437 pKa = 5.28 SNPPTRR443 pKa = 11.84 NVTGYY448 pKa = 7.88 QQPSPDD454 pKa = 4.58 DD455 pKa = 4.18 LDD457 pKa = 4.23 LLAEE461 pKa = 4.35 FEE463 pKa = 4.33 KK464 pKa = 10.95 QLTGAQDD471 pKa = 3.15 NEE473 pKa = 4.06 QRR475 pKa = 11.84 GGQTSGKK482 pKa = 10.23 VNGQNVEE489 pKa = 4.27 TTDD492 pKa = 3.34 EE493 pKa = 4.7 SEE495 pKa = 4.43 PQHH498 pKa = 4.89 QQCNIEE504 pKa = 4.28 RR505 pKa = 11.84 EE506 pKa = 4.2 PTKK509 pKa = 10.97 DD510 pKa = 2.99 KK511 pKa = 11.28 VLEE514 pKa = 4.16 HH515 pKa = 6.72 VNDD518 pKa = 2.9 ITTRR522 pKa = 11.84 SEE524 pKa = 3.93 QEE526 pKa = 3.56 GDD528 pKa = 3.49 VTDD531 pKa = 3.86 WSDD534 pKa = 4.47 DD535 pKa = 3.28 EE536 pKa = 4.86 SLAEE540 pKa = 4.77 KK541 pKa = 8.14 EE542 pKa = 4.37 THH544 pKa = 6.02 EE545 pKa = 4.06 QDD547 pKa = 4.5 APLSDD552 pKa = 5.61 DD553 pKa = 4.25 EE554 pKa = 5.94 DD555 pKa = 5.49 LFDD558 pKa = 6.37 LPPLEE563 pKa = 4.6 NCSEE567 pKa = 4.42 CGDD570 pKa = 4.78 DD571 pKa = 4.12 STQKK575 pKa = 11.0 LPDD578 pKa = 4.46 GDD580 pKa = 4.44 DD581 pKa = 3.62 CDD583 pKa = 3.88 MPNIDD588 pKa = 4.93 EE589 pKa = 4.36 EE590 pKa = 4.6 NEE592 pKa = 3.89 ATEE595 pKa = 4.5 TKK597 pKa = 10.42 VEE599 pKa = 4.07 PDD601 pKa = 3.94 GISPQEE607 pKa = 3.7 PHH609 pKa = 6.79 EE610 pKa = 4.69 DD611 pKa = 3.68 SEE613 pKa = 4.36 KK614 pKa = 11.13 GYY616 pKa = 11.55 VNNCDD621 pKa = 3.48 GSQTEE626 pKa = 4.53 HH627 pKa = 7.39 SPEE630 pKa = 5.48 HH631 pKa = 6.47 EE632 pKa = 4.88 DD633 pKa = 4.96 KK634 pKa = 10.61 PDD636 pKa = 3.43 KK637 pKa = 10.67 PVAKK641 pKa = 10.43 VKK643 pKa = 10.77 LNPLDD648 pKa = 4.99 DD649 pKa = 4.29 DD650 pKa = 4.03 TVEE653 pKa = 4.0 LLKK656 pKa = 10.68 IHH658 pKa = 6.78 GFTNFRR664 pKa = 11.84 AQYY667 pKa = 10.19 DD668 pKa = 4.18 GEE670 pKa = 4.57 DD671 pKa = 3.41 QIQPIFRR678 pKa = 11.84 NANAKK683 pKa = 10.1 FLTQYY688 pKa = 6.25 TTTVAGPQQCFIDD701 pKa = 3.83 MAHH704 pKa = 6.96 GLRR707 pKa = 11.84 RR708 pKa = 11.84 NCYY711 pKa = 8.4 EE712 pKa = 3.36 YY713 pKa = 10.67 RR714 pKa = 11.84 INAKK718 pKa = 9.99 RR719 pKa = 11.84 ATAFMSDD726 pKa = 3.27 VKK728 pKa = 11.38 NNLTGLVLPKK738 pKa = 10.42 LEE740 pKa = 4.82 RR741 pKa = 11.84 DD742 pKa = 3.88 LLSSWVSLAEE752 pKa = 3.74 HH753 pKa = 6.92 ATRR756 pKa = 11.84 KK757 pKa = 9.99 VGLIVIHH764 pKa = 6.15 GAGGAGKK771 pKa = 10.07 SRR773 pKa = 11.84 ALQEE777 pKa = 4.18 LLRR780 pKa = 11.84 SQTVDD785 pKa = 3.07 TNEE788 pKa = 3.8 VNIVVPTINLAADD801 pKa = 3.7 WKK803 pKa = 10.56 KK804 pKa = 10.73 KK805 pKa = 10.0 IPAVDD810 pKa = 3.65 PRR812 pKa = 11.84 RR813 pKa = 11.84 VMTFEE818 pKa = 4.09 KK819 pKa = 10.45 ACEE822 pKa = 4.07 RR823 pKa = 11.84 EE824 pKa = 4.26 GKK826 pKa = 9.55 PVVIMDD832 pKa = 4.47 DD833 pKa = 3.91 YY834 pKa = 10.41 EE835 pKa = 4.26 ARR837 pKa = 11.84 PAGYY841 pKa = 10.13 VDD843 pKa = 4.86 AYY845 pKa = 11.34 LSIKK849 pKa = 10.83 SNVEE853 pKa = 3.86 LVVLTGDD860 pKa = 3.43 QRR862 pKa = 11.84 QSVYY866 pKa = 11.02 HH867 pKa = 5.98 NDD869 pKa = 3.26 KK870 pKa = 10.49 RR871 pKa = 11.84 DD872 pKa = 3.5 SQIALLSSNTDD883 pKa = 3.01 HH884 pKa = 6.99 FKK886 pKa = 10.72 KK887 pKa = 10.83 YY888 pKa = 10.1 CDD890 pKa = 3.64 YY891 pKa = 10.98 YY892 pKa = 11.47 VNATHH897 pKa = 6.72 RR898 pKa = 11.84 QPRR901 pKa = 11.84 RR902 pKa = 11.84 LANPIKK908 pKa = 9.76 VHH910 pKa = 6.47 AEE912 pKa = 3.97 RR913 pKa = 11.84 EE914 pKa = 3.95 VGGAVKK920 pKa = 10.02 HH921 pKa = 5.46 ATLIPEE927 pKa = 4.35 TAMTLVPAFRR937 pKa = 11.84 SQSLLTDD944 pKa = 4.09 LGRR947 pKa = 11.84 QAMTYY952 pKa = 10.18 AGCQGLTLPHH962 pKa = 6.01 LTIVLDD968 pKa = 4.36 KK969 pKa = 10.04 DD970 pKa = 3.69 TPLCSDD976 pKa = 2.88 QVMYY980 pKa = 9.39 TALSRR985 pKa = 11.84 ASEE988 pKa = 4.34 TITFVNTHH996 pKa = 5.22 SDD998 pKa = 3.1 NRR1000 pKa = 11.84 EE1001 pKa = 3.68 FQAKK1005 pKa = 9.93 LDD1007 pKa = 3.63 STPYY1011 pKa = 10.73 LKK1013 pKa = 9.81 TLISGVRR1020 pKa = 11.84 EE1021 pKa = 4.11 DD1022 pKa = 4.44 EE1023 pKa = 4.33 LTGGDD1028 pKa = 4.04 YY1029 pKa = 10.72 KK1030 pKa = 10.92 PPEE1033 pKa = 4.76 PIVRR1037 pKa = 11.84 EE1038 pKa = 4.58 GPTKK1042 pKa = 9.79 THH1044 pKa = 6.0 MPVANDD1050 pKa = 4.32 KK1051 pKa = 10.72 IQLEE1055 pKa = 4.34 EE1056 pKa = 4.98 KK1057 pKa = 10.24 IEE1059 pKa = 4.0 AMEE1062 pKa = 5.02 DD1063 pKa = 3.08 KK1064 pKa = 9.95 DD1065 pKa = 4.96 TRR1067 pKa = 11.84 EE1068 pKa = 3.87 LWSGEE1073 pKa = 3.84 EE1074 pKa = 4.18 KK1075 pKa = 10.4 TNLMQTQDD1083 pKa = 4.55 PIVQLFPHH1091 pKa = 5.65 QQAKK1095 pKa = 10.89 DD1096 pKa = 3.24 EE1097 pKa = 4.47 ALFKK1101 pKa = 10.09 ITIKK1105 pKa = 10.57 EE1106 pKa = 4.04 RR1107 pKa = 11.84 VRR1109 pKa = 11.84 TADD1112 pKa = 3.02 ANTNRR1117 pKa = 11.84 KK1118 pKa = 8.94 AMHH1121 pKa = 5.87 EE1122 pKa = 4.12 TLNAGDD1128 pKa = 5.06 LLFEE1132 pKa = 5.12 AYY1134 pKa = 10.06 AKK1136 pKa = 10.37 FMNVPQEE1143 pKa = 3.89 TQAFDD1148 pKa = 3.2 KK1149 pKa = 10.9 RR1150 pKa = 11.84 LWAHH1154 pKa = 6.37 CRR1156 pKa = 11.84 QLALRR1161 pKa = 11.84 TYY1163 pKa = 10.57 LSKK1166 pKa = 9.68 PTSNLQQGAQRR1177 pKa = 11.84 QDD1179 pKa = 2.93 PDD1181 pKa = 3.87 FPDD1184 pKa = 3.11 NAIALFNKK1192 pKa = 8.34 SQWVKK1197 pKa = 10.35 KK1198 pKa = 10.25 LEE1200 pKa = 4.06 KK1201 pKa = 10.47 VGCKK1205 pKa = 9.95 FKK1207 pKa = 11.02 AGQTISAFKK1216 pKa = 10.55 QSTVLLTTTMALYY1229 pKa = 10.13 LRR1231 pKa = 11.84 KK1232 pKa = 9.51 KK1233 pKa = 10.63 RR1234 pKa = 11.84 EE1235 pKa = 3.53 GHH1237 pKa = 5.43 QPDD1240 pKa = 3.93 NVFVMCEE1247 pKa = 3.73 KK1248 pKa = 10.34 TPEE1251 pKa = 4.02 QFNSFVLTRR1260 pKa = 11.84 WNFKK1264 pKa = 10.2 RR1265 pKa = 11.84 PNYY1268 pKa = 9.23 TSDD1271 pKa = 3.04 YY1272 pKa = 8.03 TQYY1275 pKa = 11.28 DD1276 pKa = 3.62 QSQDD1280 pKa = 3.09 AAFLNFEE1287 pKa = 4.32 LRR1289 pKa = 11.84 KK1290 pKa = 9.67 ARR1292 pKa = 11.84 HH1293 pKa = 5.63 FGIPPEE1299 pKa = 4.18 IVDD1302 pKa = 4.42 FYY1304 pKa = 11.98 AFIKK1308 pKa = 8.86 THH1310 pKa = 6.42 AKK1312 pKa = 9.19 TFLGNLAIMRR1322 pKa = 11.84 LSGEE1326 pKa = 4.31 GPTFDD1331 pKa = 6.1 ANTEE1335 pKa = 4.05 CNIAYY1340 pKa = 8.27 DD1341 pKa = 3.69 ALRR1344 pKa = 11.84 FQVDD1348 pKa = 3.57 PTVNACYY1355 pKa = 10.28 AGDD1358 pKa = 3.77 DD1359 pKa = 3.85 LVRR1362 pKa = 11.84 DD1363 pKa = 4.11 KK1364 pKa = 11.41 ACEE1367 pKa = 3.73 EE1368 pKa = 4.09 RR1369 pKa = 11.84 PGWKK1373 pKa = 9.61 YY1374 pKa = 11.18 AEE1376 pKa = 4.31 PLFSLKK1382 pKa = 10.56 AKK1384 pKa = 10.13 PLVTNKK1390 pKa = 9.9 PDD1392 pKa = 3.19 FCGWRR1397 pKa = 11.84 LTKK1400 pKa = 10.71 YY1401 pKa = 10.46 GIVKK1405 pKa = 10.52 SPVQLYY1411 pKa = 10.28 QSLQLALRR1419 pKa = 11.84 LGKK1422 pKa = 10.19 VEE1424 pKa = 4.03 EE1425 pKa = 4.42 VKK1427 pKa = 10.49 RR1428 pKa = 11.84 SYY1430 pKa = 11.23 AIDD1433 pKa = 3.38 YY1434 pKa = 9.93 LFAYY1438 pKa = 10.43 RR1439 pKa = 11.84 LGDD1442 pKa = 3.26 QLYY1445 pKa = 10.87 DD1446 pKa = 3.48 VFDD1449 pKa = 4.15 EE1450 pKa = 4.8 NEE1452 pKa = 4.12 MEE1454 pKa = 4.04 KK1455 pKa = 10.33 HH1456 pKa = 5.01 QLVTRR1461 pKa = 11.84 TLIKK1465 pKa = 10.51 KK1466 pKa = 9.59 GMQPPSSGDD1475 pKa = 3.45 HH1476 pKa = 6.25 LPVFHH1481 pKa = 6.29 VTSDD1485 pKa = 3.13 RR1486 pKa = 11.84 LIRR1489 pKa = 11.84 DD1490 pKa = 3.89 PSAKK1494 pKa = 9.63 RR1495 pKa = 11.84 VSTYY1499 pKa = 10.53 EE1500 pKa = 3.87 YY1501 pKa = 10.73 DD1502 pKa = 3.9 SVSLPFEE1509 pKa = 5.35 LIDD1512 pKa = 4.58 DD1513 pKa = 4.58 HH1514 pKa = 6.75 FAHH1517 pKa = 6.65 NSSRR1521 pKa = 11.84 PDD1523 pKa = 3.39 RR1524 pKa = 11.84 EE1525 pKa = 4.15 DD1526 pKa = 3.05 MNNARR1531 pKa = 11.84 DD1532 pKa = 3.95 TQTQDD1537 pKa = 2.7 HH1538 pKa = 6.84 TSPAGSPEE1546 pKa = 3.46 GRR1548 pKa = 11.84 EE1549 pKa = 3.99 PLLSDD1554 pKa = 5.19 LFPQLISLL1562 pKa = 4.53
Molecular weight: 176.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.564
IPC2_protein 5.499
IPC_protein 5.55
Toseland 5.881
ProMoST 5.855
Dawson 5.753
Bjellqvist 5.792
Wikipedia 5.728
Rodwell 5.715
Grimsley 6.008
Solomon 5.753
Lehninger 5.728
Nozaki 5.982
DTASelect 6.173
Thurlkill 6.135
EMBOSS 6.097
Sillero 6.084
Patrickios 4.139
IPC_peptide 5.766
IPC2_peptide 6.084
IPC2.peptide.svr19 6.032
Protein with the highest isoelectric point:
>tr|Q9Z013|Q9Z013_9VIRU 11kDa protein OS=Garlic virus D OX=12430 PE=4 SV=1
MM1 pKa = 7.51 HH2 pKa = 7.32 PHH4 pKa = 7.33 DD5 pKa = 5.95 FNLLCCLHH13 pKa = 6.82 FSQPPLPKK21 pKa = 10.01 DD22 pKa = 3.21 LKK24 pKa = 10.05 EE25 pKa = 4.81 FIFSRR30 pKa = 11.84 CVDD33 pKa = 3.35 CCKK36 pKa = 10.34 LVRR39 pKa = 11.84 RR40 pKa = 11.84 TNQNKK45 pKa = 8.93 PFQGVSKK52 pKa = 9.92 CAKK55 pKa = 8.37 RR56 pKa = 11.84 RR57 pKa = 11.84 RR58 pKa = 11.84 AKK60 pKa = 10.29 RR61 pKa = 11.84 YY62 pKa = 8.53 NRR64 pKa = 11.84 CFDD67 pKa = 3.69 CGAFLVDD74 pKa = 3.04 GHH76 pKa = 5.87 RR77 pKa = 11.84 CKK79 pKa = 11.06 VFVSKK84 pKa = 10.75 AHH86 pKa = 6.19 SDD88 pKa = 3.55 VLAVIHH94 pKa = 6.42 EE95 pKa = 4.96 GPAKK99 pKa = 10.37 LYY101 pKa = 10.79 AEE103 pKa = 4.2 RR104 pKa = 11.84 SYY106 pKa = 11.42 RR107 pKa = 11.84 PNSDD111 pKa = 2.9 AAQLIEE117 pKa = 4.92 NDD119 pKa = 3.02 ILYY122 pKa = 10.08 IRR124 pKa = 11.84 ALKK127 pKa = 10.34 LL128 pKa = 3.22
Molecular weight: 14.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.901
IPC2_protein 8.624
IPC_protein 8.536
Toseland 9.077
ProMoST 9.063
Dawson 9.443
Bjellqvist 9.458
Wikipedia 9.531
Rodwell 9.648
Grimsley 9.311
Solomon 9.545
Lehninger 9.531
Nozaki 9.736
DTASelect 9.268
Thurlkill 9.414
EMBOSS 9.633
Sillero 9.619
Patrickios 4.94
IPC_peptide 9.545
IPC2_peptide 8.624
IPC2.peptide.svr19 7.834
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2644
103
1562
440.7
49.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.224 ± 0.325
2.005 ± 0.385
6.505 ± 0.48
5.673 ± 0.917
3.896 ± 0.365
4.955 ± 0.562
3.328 ± 0.476
4.576 ± 0.257
5.182 ± 0.796
10.136 ± 0.811
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.589 ± 0.286
4.463 ± 0.393
6.543 ± 0.49
4.803 ± 0.346
5.862 ± 0.267
6.354 ± 0.708
7.262 ± 0.574
5.598 ± 0.405
0.681 ± 0.157
3.366 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here