Garlic virus D

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Allexivirus; Acarallexivirus

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|V5NX44|V5NX44_9VIRU RNA-directed RNA polymerase OS=Garlic virus D OX=12430 PE=4 SV=1
MM1 pKa = 7.07GTVQKK6 pKa = 10.9LLDD9 pKa = 4.13QISDD13 pKa = 3.73PNARR17 pKa = 11.84AAYY20 pKa = 10.16SQDD23 pKa = 3.38CLSAAKK29 pKa = 10.04AAATKK34 pKa = 10.98AMTIAPYY41 pKa = 10.46AVNQTEE47 pKa = 4.18ALTLEE52 pKa = 4.81RR53 pKa = 11.84IGITTSPFATTSHH66 pKa = 4.89THH68 pKa = 5.93AADD71 pKa = 4.1KK72 pKa = 10.63IIEE75 pKa = 4.28NDD77 pKa = 3.51CLDD80 pKa = 3.67IIGRR84 pKa = 11.84YY85 pKa = 8.46LPKK88 pKa = 10.68EE89 pKa = 4.03PVTLIQLKK97 pKa = 9.75RR98 pKa = 11.84AKK100 pKa = 10.22LHH102 pKa = 5.63LLRR105 pKa = 11.84RR106 pKa = 11.84GPSGDD111 pKa = 2.8IFKK114 pKa = 10.65NYY116 pKa = 9.13CHH118 pKa = 6.56EE119 pKa = 4.62PKK121 pKa = 10.43DD122 pKa = 3.62VLRR125 pKa = 11.84FGITNPHH132 pKa = 5.96VCPQVNTTCAVISDD146 pKa = 4.21TLHH149 pKa = 6.6FMSTQQVWTLFQNNPSLEE167 pKa = 4.11RR168 pKa = 11.84LYY170 pKa = 10.37GTLVLPIEE178 pKa = 4.47ALHH181 pKa = 6.34RR182 pKa = 11.84LPSLFPEE189 pKa = 4.68IYY191 pKa = 10.22KK192 pKa = 10.54LEE194 pKa = 4.4YY195 pKa = 9.26YY196 pKa = 9.99DD197 pKa = 4.11KK198 pKa = 10.55HH199 pKa = 5.63FAYY202 pKa = 9.87IPGRR206 pKa = 11.84HH207 pKa = 5.8GGGAYY212 pKa = 8.62VHH214 pKa = 6.94SYY216 pKa = 8.51GTLKK220 pKa = 10.22WLSTAQIGPGPYY232 pKa = 9.24PEE234 pKa = 4.37YY235 pKa = 10.33RR236 pKa = 11.84SNANGRR242 pKa = 11.84RR243 pKa = 11.84PLLDD247 pKa = 3.52HH248 pKa = 6.95LSVEE252 pKa = 4.38KK253 pKa = 10.75IEE255 pKa = 4.43TKK257 pKa = 10.11AAHH260 pKa = 6.64HH261 pKa = 6.39IVLIQRR267 pKa = 11.84RR268 pKa = 11.84RR269 pKa = 11.84PEE271 pKa = 4.56LPWPLPPGWVYY282 pKa = 10.57HH283 pKa = 6.34ASDD286 pKa = 4.03YY287 pKa = 11.61VKK289 pKa = 10.48LPEE292 pKa = 3.88IFYY295 pKa = 9.44PAEE298 pKa = 4.09ANVQKK303 pKa = 10.47AYY305 pKa = 9.92PHH307 pKa = 6.42IFIKK311 pKa = 10.52RR312 pKa = 11.84LQLYY316 pKa = 8.31CFSVKK321 pKa = 10.2AVSLRR326 pKa = 11.84DD327 pKa = 3.18IFAKK331 pKa = 9.72IRR333 pKa = 11.84QVIPTEE339 pKa = 3.68EE340 pKa = 4.04LARR343 pKa = 11.84FSTADD348 pKa = 4.67LIRR351 pKa = 11.84LANYY355 pKa = 10.44LLFITGMQSTSDD367 pKa = 3.71YY368 pKa = 10.66QSPLMEE374 pKa = 4.71SLLGKK379 pKa = 10.15LCLSIRR385 pKa = 11.84ARR387 pKa = 11.84VRR389 pKa = 11.84EE390 pKa = 4.06FFQNILGKK398 pKa = 10.11SSYY401 pKa = 10.85AALLTVTDD409 pKa = 3.35VRR411 pKa = 11.84PIYY414 pKa = 9.46FTTTPRR420 pKa = 11.84RR421 pKa = 11.84RR422 pKa = 11.84KK423 pKa = 9.28ICGEE427 pKa = 3.59AWFDD431 pKa = 3.59SLEE434 pKa = 4.24EE435 pKa = 4.29EE436 pKa = 4.74EE437 pKa = 5.28SNPPTRR443 pKa = 11.84NVTGYY448 pKa = 7.88QQPSPDD454 pKa = 4.58DD455 pKa = 4.18LDD457 pKa = 4.23LLAEE461 pKa = 4.35FEE463 pKa = 4.33KK464 pKa = 10.95QLTGAQDD471 pKa = 3.15NEE473 pKa = 4.06QRR475 pKa = 11.84GGQTSGKK482 pKa = 10.23VNGQNVEE489 pKa = 4.27TTDD492 pKa = 3.34EE493 pKa = 4.7SEE495 pKa = 4.43PQHH498 pKa = 4.89QQCNIEE504 pKa = 4.28RR505 pKa = 11.84EE506 pKa = 4.2PTKK509 pKa = 10.97DD510 pKa = 2.99KK511 pKa = 11.28VLEE514 pKa = 4.16HH515 pKa = 6.72VNDD518 pKa = 2.9ITTRR522 pKa = 11.84SEE524 pKa = 3.93QEE526 pKa = 3.56GDD528 pKa = 3.49VTDD531 pKa = 3.86WSDD534 pKa = 4.47DD535 pKa = 3.28EE536 pKa = 4.86SLAEE540 pKa = 4.77KK541 pKa = 8.14EE542 pKa = 4.37THH544 pKa = 6.02EE545 pKa = 4.06QDD547 pKa = 4.5APLSDD552 pKa = 5.61DD553 pKa = 4.25EE554 pKa = 5.94DD555 pKa = 5.49LFDD558 pKa = 6.37LPPLEE563 pKa = 4.6NCSEE567 pKa = 4.42CGDD570 pKa = 4.78DD571 pKa = 4.12STQKK575 pKa = 11.0LPDD578 pKa = 4.46GDD580 pKa = 4.44DD581 pKa = 3.62CDD583 pKa = 3.88MPNIDD588 pKa = 4.93EE589 pKa = 4.36EE590 pKa = 4.6NEE592 pKa = 3.89ATEE595 pKa = 4.5TKK597 pKa = 10.42VEE599 pKa = 4.07PDD601 pKa = 3.94GISPQEE607 pKa = 3.7PHH609 pKa = 6.79EE610 pKa = 4.69DD611 pKa = 3.68SEE613 pKa = 4.36KK614 pKa = 11.13GYY616 pKa = 11.55VNNCDD621 pKa = 3.48GSQTEE626 pKa = 4.53HH627 pKa = 7.39SPEE630 pKa = 5.48HH631 pKa = 6.47EE632 pKa = 4.88DD633 pKa = 4.96KK634 pKa = 10.61PDD636 pKa = 3.43KK637 pKa = 10.67PVAKK641 pKa = 10.43VKK643 pKa = 10.77LNPLDD648 pKa = 4.99DD649 pKa = 4.29DD650 pKa = 4.03TVEE653 pKa = 4.0LLKK656 pKa = 10.68IHH658 pKa = 6.78GFTNFRR664 pKa = 11.84AQYY667 pKa = 10.19DD668 pKa = 4.18GEE670 pKa = 4.57DD671 pKa = 3.41QIQPIFRR678 pKa = 11.84NANAKK683 pKa = 10.1FLTQYY688 pKa = 6.25TTTVAGPQQCFIDD701 pKa = 3.83MAHH704 pKa = 6.96GLRR707 pKa = 11.84RR708 pKa = 11.84NCYY711 pKa = 8.4EE712 pKa = 3.36YY713 pKa = 10.67RR714 pKa = 11.84INAKK718 pKa = 9.99RR719 pKa = 11.84ATAFMSDD726 pKa = 3.27VKK728 pKa = 11.38NNLTGLVLPKK738 pKa = 10.42LEE740 pKa = 4.82RR741 pKa = 11.84DD742 pKa = 3.88LLSSWVSLAEE752 pKa = 3.74HH753 pKa = 6.92ATRR756 pKa = 11.84KK757 pKa = 9.99VGLIVIHH764 pKa = 6.15GAGGAGKK771 pKa = 10.07SRR773 pKa = 11.84ALQEE777 pKa = 4.18LLRR780 pKa = 11.84SQTVDD785 pKa = 3.07TNEE788 pKa = 3.8VNIVVPTINLAADD801 pKa = 3.7WKK803 pKa = 10.56KK804 pKa = 10.73KK805 pKa = 10.0IPAVDD810 pKa = 3.65PRR812 pKa = 11.84RR813 pKa = 11.84VMTFEE818 pKa = 4.09KK819 pKa = 10.45ACEE822 pKa = 4.07RR823 pKa = 11.84EE824 pKa = 4.26GKK826 pKa = 9.55PVVIMDD832 pKa = 4.47DD833 pKa = 3.91YY834 pKa = 10.41EE835 pKa = 4.26ARR837 pKa = 11.84PAGYY841 pKa = 10.13VDD843 pKa = 4.86AYY845 pKa = 11.34LSIKK849 pKa = 10.83SNVEE853 pKa = 3.86LVVLTGDD860 pKa = 3.43QRR862 pKa = 11.84QSVYY866 pKa = 11.02HH867 pKa = 5.98NDD869 pKa = 3.26KK870 pKa = 10.49RR871 pKa = 11.84DD872 pKa = 3.5SQIALLSSNTDD883 pKa = 3.01HH884 pKa = 6.99FKK886 pKa = 10.72KK887 pKa = 10.83YY888 pKa = 10.1CDD890 pKa = 3.64YY891 pKa = 10.98YY892 pKa = 11.47VNATHH897 pKa = 6.72RR898 pKa = 11.84QPRR901 pKa = 11.84RR902 pKa = 11.84LANPIKK908 pKa = 9.76VHH910 pKa = 6.47AEE912 pKa = 3.97RR913 pKa = 11.84EE914 pKa = 3.95VGGAVKK920 pKa = 10.02HH921 pKa = 5.46ATLIPEE927 pKa = 4.35TAMTLVPAFRR937 pKa = 11.84SQSLLTDD944 pKa = 4.09LGRR947 pKa = 11.84QAMTYY952 pKa = 10.18AGCQGLTLPHH962 pKa = 6.01LTIVLDD968 pKa = 4.36KK969 pKa = 10.04DD970 pKa = 3.69TPLCSDD976 pKa = 2.88QVMYY980 pKa = 9.39TALSRR985 pKa = 11.84ASEE988 pKa = 4.34TITFVNTHH996 pKa = 5.22SDD998 pKa = 3.1NRR1000 pKa = 11.84EE1001 pKa = 3.68FQAKK1005 pKa = 9.93LDD1007 pKa = 3.63STPYY1011 pKa = 10.73LKK1013 pKa = 9.81TLISGVRR1020 pKa = 11.84EE1021 pKa = 4.11DD1022 pKa = 4.44EE1023 pKa = 4.33LTGGDD1028 pKa = 4.04YY1029 pKa = 10.72KK1030 pKa = 10.92PPEE1033 pKa = 4.76PIVRR1037 pKa = 11.84EE1038 pKa = 4.58GPTKK1042 pKa = 9.79THH1044 pKa = 6.0MPVANDD1050 pKa = 4.32KK1051 pKa = 10.72IQLEE1055 pKa = 4.34EE1056 pKa = 4.98KK1057 pKa = 10.24IEE1059 pKa = 4.0AMEE1062 pKa = 5.02DD1063 pKa = 3.08KK1064 pKa = 9.95DD1065 pKa = 4.96TRR1067 pKa = 11.84EE1068 pKa = 3.87LWSGEE1073 pKa = 3.84EE1074 pKa = 4.18KK1075 pKa = 10.4TNLMQTQDD1083 pKa = 4.55PIVQLFPHH1091 pKa = 5.65QQAKK1095 pKa = 10.89DD1096 pKa = 3.24EE1097 pKa = 4.47ALFKK1101 pKa = 10.09ITIKK1105 pKa = 10.57EE1106 pKa = 4.04RR1107 pKa = 11.84VRR1109 pKa = 11.84TADD1112 pKa = 3.02ANTNRR1117 pKa = 11.84KK1118 pKa = 8.94AMHH1121 pKa = 5.87EE1122 pKa = 4.12TLNAGDD1128 pKa = 5.06LLFEE1132 pKa = 5.12AYY1134 pKa = 10.06AKK1136 pKa = 10.37FMNVPQEE1143 pKa = 3.89TQAFDD1148 pKa = 3.2KK1149 pKa = 10.9RR1150 pKa = 11.84LWAHH1154 pKa = 6.37CRR1156 pKa = 11.84QLALRR1161 pKa = 11.84TYY1163 pKa = 10.57LSKK1166 pKa = 9.68PTSNLQQGAQRR1177 pKa = 11.84QDD1179 pKa = 2.93PDD1181 pKa = 3.87FPDD1184 pKa = 3.11NAIALFNKK1192 pKa = 8.34SQWVKK1197 pKa = 10.35KK1198 pKa = 10.25LEE1200 pKa = 4.06KK1201 pKa = 10.47VGCKK1205 pKa = 9.95FKK1207 pKa = 11.02AGQTISAFKK1216 pKa = 10.55QSTVLLTTTMALYY1229 pKa = 10.13LRR1231 pKa = 11.84KK1232 pKa = 9.51KK1233 pKa = 10.63RR1234 pKa = 11.84EE1235 pKa = 3.53GHH1237 pKa = 5.43QPDD1240 pKa = 3.93NVFVMCEE1247 pKa = 3.73KK1248 pKa = 10.34TPEE1251 pKa = 4.02QFNSFVLTRR1260 pKa = 11.84WNFKK1264 pKa = 10.2RR1265 pKa = 11.84PNYY1268 pKa = 9.23TSDD1271 pKa = 3.04YY1272 pKa = 8.03TQYY1275 pKa = 11.28DD1276 pKa = 3.62QSQDD1280 pKa = 3.09AAFLNFEE1287 pKa = 4.32LRR1289 pKa = 11.84KK1290 pKa = 9.67ARR1292 pKa = 11.84HH1293 pKa = 5.63FGIPPEE1299 pKa = 4.18IVDD1302 pKa = 4.42FYY1304 pKa = 11.98AFIKK1308 pKa = 8.86THH1310 pKa = 6.42AKK1312 pKa = 9.19TFLGNLAIMRR1322 pKa = 11.84LSGEE1326 pKa = 4.31GPTFDD1331 pKa = 6.1ANTEE1335 pKa = 4.05CNIAYY1340 pKa = 8.27DD1341 pKa = 3.69ALRR1344 pKa = 11.84FQVDD1348 pKa = 3.57PTVNACYY1355 pKa = 10.28AGDD1358 pKa = 3.77DD1359 pKa = 3.85LVRR1362 pKa = 11.84DD1363 pKa = 4.11KK1364 pKa = 11.41ACEE1367 pKa = 3.73EE1368 pKa = 4.09RR1369 pKa = 11.84PGWKK1373 pKa = 9.61YY1374 pKa = 11.18AEE1376 pKa = 4.31PLFSLKK1382 pKa = 10.56AKK1384 pKa = 10.13PLVTNKK1390 pKa = 9.9PDD1392 pKa = 3.19FCGWRR1397 pKa = 11.84LTKK1400 pKa = 10.71YY1401 pKa = 10.46GIVKK1405 pKa = 10.52SPVQLYY1411 pKa = 10.28QSLQLALRR1419 pKa = 11.84LGKK1422 pKa = 10.19VEE1424 pKa = 4.03EE1425 pKa = 4.42VKK1427 pKa = 10.49RR1428 pKa = 11.84SYY1430 pKa = 11.23AIDD1433 pKa = 3.38YY1434 pKa = 9.93LFAYY1438 pKa = 10.43RR1439 pKa = 11.84LGDD1442 pKa = 3.26QLYY1445 pKa = 10.87DD1446 pKa = 3.48VFDD1449 pKa = 4.15EE1450 pKa = 4.8NEE1452 pKa = 4.12MEE1454 pKa = 4.04KK1455 pKa = 10.33HH1456 pKa = 5.01QLVTRR1461 pKa = 11.84TLIKK1465 pKa = 10.51KK1466 pKa = 9.59GMQPPSSGDD1475 pKa = 3.45HH1476 pKa = 6.25LPVFHH1481 pKa = 6.29VTSDD1485 pKa = 3.13RR1486 pKa = 11.84LIRR1489 pKa = 11.84DD1490 pKa = 3.89PSAKK1494 pKa = 9.63RR1495 pKa = 11.84VSTYY1499 pKa = 10.53EE1500 pKa = 3.87YY1501 pKa = 10.73DD1502 pKa = 3.9SVSLPFEE1509 pKa = 5.35LIDD1512 pKa = 4.58DD1513 pKa = 4.58HH1514 pKa = 6.75FAHH1517 pKa = 6.65NSSRR1521 pKa = 11.84PDD1523 pKa = 3.39RR1524 pKa = 11.84EE1525 pKa = 4.15DD1526 pKa = 3.05MNNARR1531 pKa = 11.84DD1532 pKa = 3.95TQTQDD1537 pKa = 2.7HH1538 pKa = 6.84TSPAGSPEE1546 pKa = 3.46GRR1548 pKa = 11.84EE1549 pKa = 3.99PLLSDD1554 pKa = 5.19LFPQLISLL1562 pKa = 4.53

Molecular weight:
176.91 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q9Z013|Q9Z013_9VIRU 11kDa protein OS=Garlic virus D OX=12430 PE=4 SV=1
MM1 pKa = 7.51HH2 pKa = 7.32PHH4 pKa = 7.33DD5 pKa = 5.95FNLLCCLHH13 pKa = 6.82FSQPPLPKK21 pKa = 10.01DD22 pKa = 3.21LKK24 pKa = 10.05EE25 pKa = 4.81FIFSRR30 pKa = 11.84CVDD33 pKa = 3.35CCKK36 pKa = 10.34LVRR39 pKa = 11.84RR40 pKa = 11.84TNQNKK45 pKa = 8.93PFQGVSKK52 pKa = 9.92CAKK55 pKa = 8.37RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84AKK60 pKa = 10.29RR61 pKa = 11.84YY62 pKa = 8.53NRR64 pKa = 11.84CFDD67 pKa = 3.69CGAFLVDD74 pKa = 3.04GHH76 pKa = 5.87RR77 pKa = 11.84CKK79 pKa = 11.06VFVSKK84 pKa = 10.75AHH86 pKa = 6.19SDD88 pKa = 3.55VLAVIHH94 pKa = 6.42EE95 pKa = 4.96GPAKK99 pKa = 10.37LYY101 pKa = 10.79AEE103 pKa = 4.2RR104 pKa = 11.84SYY106 pKa = 11.42RR107 pKa = 11.84PNSDD111 pKa = 2.9AAQLIEE117 pKa = 4.92NDD119 pKa = 3.02ILYY122 pKa = 10.08IRR124 pKa = 11.84ALKK127 pKa = 10.34LL128 pKa = 3.22

Molecular weight:
14.81 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2644

103

1562

440.7

49.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.224 ± 0.325

2.005 ± 0.385

6.505 ± 0.48

5.673 ± 0.917

3.896 ± 0.365

4.955 ± 0.562

3.328 ± 0.476

4.576 ± 0.257

5.182 ± 0.796

10.136 ± 0.811

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.589 ± 0.286

4.463 ± 0.393

6.543 ± 0.49

4.803 ± 0.346

5.862 ± 0.267

6.354 ± 0.708

7.262 ± 0.574

5.598 ± 0.405

0.681 ± 0.157

3.366 ± 0.344

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski