Beihai narna-like virus 4
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KI50|A0A1L3KI50_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 4 OX=1922456 PE=4 SV=1
MM1 pKa = 7.66 PVLSTLRR8 pKa = 11.84 IACVGYY14 pKa = 10.7 DD15 pKa = 3.33 PTKK18 pKa = 10.64 EE19 pKa = 4.0 YY20 pKa = 11.14 LEE22 pKa = 3.98 QALAEE27 pKa = 4.24 RR28 pKa = 11.84 TARR31 pKa = 11.84 MAGDD35 pKa = 4.2 IPCASDD41 pKa = 3.19 VDD43 pKa = 4.18 EE44 pKa = 5.61 SSDD47 pKa = 4.08 GPGVCADD54 pKa = 4.34 AYY56 pKa = 10.88 LHH58 pKa = 7.4 GIRR61 pKa = 11.84 QMDD64 pKa = 3.57 RR65 pKa = 11.84 VEE67 pKa = 4.46 EE68 pKa = 4.38 VWSHH72 pKa = 6.58 IYY74 pKa = 9.71 RR75 pKa = 11.84 CYY77 pKa = 10.69 EE78 pKa = 3.56 IDD80 pKa = 3.3 PTPFSEE86 pKa = 4.25 RR87 pKa = 11.84 DD88 pKa = 2.98 RR89 pKa = 11.84 GLFLWLGAHH98 pKa = 6.65 DD99 pKa = 3.96 QLEE102 pKa = 4.84 SYY104 pKa = 10.17 LKK106 pKa = 10.26 WRR108 pKa = 11.84 TAWLLAQVWEE118 pKa = 4.14 QRR120 pKa = 11.84 EE121 pKa = 4.38 LPSKK125 pKa = 10.29 PGYY128 pKa = 10.12 LSEE131 pKa = 4.58 VPNCYY136 pKa = 9.79 RR137 pKa = 11.84 PPRR140 pKa = 11.84 VGLTWGDD147 pKa = 3.49 SAFWQRR153 pKa = 11.84 AIHH156 pKa = 5.53 QSGNIPLRR164 pKa = 11.84 LQEE167 pKa = 4.26 CAYY170 pKa = 10.84 ALYY173 pKa = 10.05 QGKK176 pKa = 10.2 AGALPMRR183 pKa = 11.84 PDD185 pKa = 3.3 LVDD188 pKa = 3.75 AKK190 pKa = 11.03 VEE192 pKa = 4.03 EE193 pKa = 4.45 AMSRR197 pKa = 11.84 LTTPCRR203 pKa = 11.84 TRR205 pKa = 11.84 SQVIGGRR212 pKa = 11.84 KK213 pKa = 7.47 VTLQDD218 pKa = 3.38 LKK220 pKa = 10.73 EE221 pKa = 4.11 QVTRR225 pKa = 11.84 TVKK228 pKa = 10.45 EE229 pKa = 3.64 IYY231 pKa = 9.44 GPSMEE236 pKa = 4.92 EE237 pKa = 3.79 EE238 pKa = 4.41 VPTPGSHH245 pKa = 7.27 RR246 pKa = 11.84 LGSTKK251 pKa = 10.48 SSFQCPRR258 pKa = 11.84 GGGGAHH264 pKa = 5.39 QALVEE269 pKa = 4.1 EE270 pKa = 4.78 AGEE273 pKa = 4.23 GRR275 pKa = 11.84 AGVLGLPQLVGYY287 pKa = 7.73 TRR289 pKa = 11.84 EE290 pKa = 3.9 RR291 pKa = 11.84 DD292 pKa = 3.64 PVPVYY297 pKa = 10.24 STTDD301 pKa = 3.13 HH302 pKa = 6.88 LEE304 pKa = 3.69 WSHH307 pKa = 5.51 TVEE310 pKa = 3.82 ASKK313 pKa = 11.0 VLSFGEE319 pKa = 4.31 TMACYY324 pKa = 9.32 PVGLVEE330 pKa = 4.06 PFKK333 pKa = 11.27 VRR335 pKa = 11.84 VITRR339 pKa = 11.84 GAAHH343 pKa = 7.39 HH344 pKa = 5.49 YY345 pKa = 9.71 HH346 pKa = 7.26 LARR349 pKa = 11.84 RR350 pKa = 11.84 WQPSMWRR357 pKa = 11.84 PLAAHH362 pKa = 6.54 PTFQLVGRR370 pKa = 11.84 PCGRR374 pKa = 11.84 DD375 pKa = 3.17 VMYY378 pKa = 10.99 DD379 pKa = 3.29 LVRR382 pKa = 11.84 KK383 pKa = 9.99 VDD385 pKa = 4.08 FGDD388 pKa = 3.24 GRR390 pKa = 11.84 LWMSGDD396 pKa = 3.51 YY397 pKa = 10.04 QAATDD402 pKa = 3.96 YY403 pKa = 10.98 FDD405 pKa = 5.43 PEE407 pKa = 4.39 LSGHH411 pKa = 6.36 CLMEE415 pKa = 4.41 VCDD418 pKa = 4.11 SLGVPWEE425 pKa = 3.86 DD426 pKa = 3.38 RR427 pKa = 11.84 IVLRR431 pKa = 11.84 RR432 pKa = 11.84 ALIGHH437 pKa = 6.29 VFHH440 pKa = 7.84 DD441 pKa = 4.77 PDD443 pKa = 3.3 THH445 pKa = 6.9 EE446 pKa = 4.38 YY447 pKa = 10.5 LGHH450 pKa = 5.19 QARR453 pKa = 11.84 GQLMGSPVSFPILCLLNAALTRR475 pKa = 11.84 FAFEE479 pKa = 3.76 LAGRR483 pKa = 11.84 EE484 pKa = 3.85 ASMLRR489 pKa = 11.84 DD490 pKa = 3.34 QPILVNGDD498 pKa = 3.88 DD499 pKa = 4.15 LLCRR503 pKa = 11.84 TSDD506 pKa = 3.04 KK507 pKa = 10.87 EE508 pKa = 4.09 YY509 pKa = 10.73 YY510 pKa = 9.33 IWSQITAMGGLIPSLGKK527 pKa = 10.18 CFRR530 pKa = 11.84 HH531 pKa = 6.41 RR532 pKa = 11.84 RR533 pKa = 11.84 IATINSEE540 pKa = 3.72 MWVCRR545 pKa = 11.84 KK546 pKa = 8.49 MRR548 pKa = 11.84 TTLLGPLDD556 pKa = 4.31 GPLSEE561 pKa = 4.56 FTYY564 pKa = 8.21 TTIEE568 pKa = 4.07 RR569 pKa = 11.84 RR570 pKa = 11.84 HH571 pKa = 6.06 LPLEE575 pKa = 4.11 GLARR579 pKa = 11.84 GSIKK583 pKa = 10.46 GASGTTDD590 pKa = 2.81 SQRR593 pKa = 11.84 RR594 pKa = 11.84 IEE596 pKa = 5.07 KK597 pKa = 9.78 MSLLDD602 pKa = 3.59 SSNVRR607 pKa = 11.84 SLVPMGDD614 pKa = 2.98 CWRR617 pKa = 11.84 EE618 pKa = 3.97 YY619 pKa = 10.71 VRR621 pKa = 11.84 STPSYY626 pKa = 10.84 ADD628 pKa = 3.22 EE629 pKa = 4.44 SSPGVRR635 pKa = 11.84 AALQRR640 pKa = 11.84 RR641 pKa = 11.84 LWDD644 pKa = 3.27 HH645 pKa = 6.27 CWDD648 pKa = 3.7 VNRR651 pKa = 11.84 DD652 pKa = 3.24 RR653 pKa = 11.84 LKK655 pKa = 10.61 RR656 pKa = 11.84 ISRR659 pKa = 11.84 FPISFCMPVRR669 pKa = 11.84 YY670 pKa = 9.72 GGLGFPLPPPSSRR683 pKa = 11.84 SYY685 pKa = 10.97 AQRR688 pKa = 11.84 LPKK691 pKa = 10.17 ANNWAMANLLANRR704 pKa = 11.84 PNLRR708 pKa = 11.84 RR709 pKa = 11.84 ALLSQLKK716 pKa = 9.98 PDD718 pKa = 3.69 VPCYY722 pKa = 10.97 GDD724 pKa = 2.96 VAYY727 pKa = 9.58 PLRR730 pKa = 11.84 CVPNRR735 pKa = 11.84 DD736 pKa = 3.37 ANLDD740 pKa = 3.58 EE741 pKa = 5.38 EE742 pKa = 4.74 YY743 pKa = 11.17 DD744 pKa = 3.76 SFRR747 pKa = 11.84 RR748 pKa = 11.84 SYY750 pKa = 11.03 PDD752 pKa = 3.61 LPPEE756 pKa = 4.03 SFAPRR761 pKa = 11.84 RR762 pKa = 11.84 GLPSRR767 pKa = 11.84 PSTDD771 pKa = 2.87 PSFWGMCGSEE781 pKa = 3.77 PTQYY785 pKa = 11.35 YY786 pKa = 10.35 EE787 pKa = 4.96 DD788 pKa = 4.34 DD789 pKa = 3.78 SGFLRR794 pKa = 11.84 IWNAANRR801 pKa = 11.84 IAQSKK806 pKa = 9.53 NSSGKK811 pKa = 9.4 YY812 pKa = 7.88 RR813 pKa = 11.84 WAPLRR818 pKa = 11.84 RR819 pKa = 11.84 LSLLDD824 pKa = 3.87 TIALSDD830 pKa = 3.78 HH831 pKa = 6.7 KK832 pKa = 10.73 LTGLDD837 pKa = 4.42 LPHH840 pKa = 7.71 SDD842 pKa = 2.42 IRR844 pKa = 11.84 SPFRR848 pKa = 11.84 DD849 pKa = 3.15 TGVSRR854 pKa = 11.84 HH855 pKa = 5.37 EE856 pKa = 4.01 VAALGDD862 pKa = 3.78 VVLNEE867 pKa = 4.11 LWW869 pKa = 3.59
Molecular weight: 98.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.843
IPC2_protein 7.044
IPC_protein 6.927
Toseland 6.751
ProMoST 7.483
Dawson 7.556
Bjellqvist 7.966
Wikipedia 7.483
Rodwell 7.571
Grimsley 6.839
Solomon 7.571
Lehninger 7.6
Nozaki 8.2
DTASelect 7.775
Thurlkill 7.819
EMBOSS 7.819
Sillero 8.097
Patrickios 4.202
IPC_peptide 7.571
IPC2_peptide 7.468
IPC2.peptide.svr19 7.119
Protein with the highest isoelectric point:
>tr|A0A1L3KI50|A0A1L3KI50_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 4 OX=1922456 PE=4 SV=1
MM1 pKa = 7.66 PVLSTLRR8 pKa = 11.84 IACVGYY14 pKa = 10.7 DD15 pKa = 3.33 PTKK18 pKa = 10.64 EE19 pKa = 4.0 YY20 pKa = 11.14 LEE22 pKa = 3.98 QALAEE27 pKa = 4.24 RR28 pKa = 11.84 TARR31 pKa = 11.84 MAGDD35 pKa = 4.2 IPCASDD41 pKa = 3.19 VDD43 pKa = 4.18 EE44 pKa = 5.61 SSDD47 pKa = 4.08 GPGVCADD54 pKa = 4.34 AYY56 pKa = 10.88 LHH58 pKa = 7.4 GIRR61 pKa = 11.84 QMDD64 pKa = 3.57 RR65 pKa = 11.84 VEE67 pKa = 4.46 EE68 pKa = 4.38 VWSHH72 pKa = 6.58 IYY74 pKa = 9.71 RR75 pKa = 11.84 CYY77 pKa = 10.69 EE78 pKa = 3.56 IDD80 pKa = 3.3 PTPFSEE86 pKa = 4.25 RR87 pKa = 11.84 DD88 pKa = 2.98 RR89 pKa = 11.84 GLFLWLGAHH98 pKa = 6.65 DD99 pKa = 3.96 QLEE102 pKa = 4.84 SYY104 pKa = 10.17 LKK106 pKa = 10.26 WRR108 pKa = 11.84 TAWLLAQVWEE118 pKa = 4.14 QRR120 pKa = 11.84 EE121 pKa = 4.38 LPSKK125 pKa = 10.29 PGYY128 pKa = 10.12 LSEE131 pKa = 4.58 VPNCYY136 pKa = 9.79 RR137 pKa = 11.84 PPRR140 pKa = 11.84 VGLTWGDD147 pKa = 3.49 SAFWQRR153 pKa = 11.84 AIHH156 pKa = 5.53 QSGNIPLRR164 pKa = 11.84 LQEE167 pKa = 4.26 CAYY170 pKa = 10.84 ALYY173 pKa = 10.05 QGKK176 pKa = 10.2 AGALPMRR183 pKa = 11.84 PDD185 pKa = 3.3 LVDD188 pKa = 3.75 AKK190 pKa = 11.03 VEE192 pKa = 4.03 EE193 pKa = 4.45 AMSRR197 pKa = 11.84 LTTPCRR203 pKa = 11.84 TRR205 pKa = 11.84 SQVIGGRR212 pKa = 11.84 KK213 pKa = 7.47 VTLQDD218 pKa = 3.38 LKK220 pKa = 10.73 EE221 pKa = 4.11 QVTRR225 pKa = 11.84 TVKK228 pKa = 10.45 EE229 pKa = 3.64 IYY231 pKa = 9.44 GPSMEE236 pKa = 4.92 EE237 pKa = 3.79 EE238 pKa = 4.41 VPTPGSHH245 pKa = 7.27 RR246 pKa = 11.84 LGSTKK251 pKa = 10.48 SSFQCPRR258 pKa = 11.84 GGGGAHH264 pKa = 5.39 QALVEE269 pKa = 4.1 EE270 pKa = 4.78 AGEE273 pKa = 4.23 GRR275 pKa = 11.84 AGVLGLPQLVGYY287 pKa = 7.73 TRR289 pKa = 11.84 EE290 pKa = 3.9 RR291 pKa = 11.84 DD292 pKa = 3.64 PVPVYY297 pKa = 10.24 STTDD301 pKa = 3.13 HH302 pKa = 6.88 LEE304 pKa = 3.69 WSHH307 pKa = 5.51 TVEE310 pKa = 3.82 ASKK313 pKa = 11.0 VLSFGEE319 pKa = 4.31 TMACYY324 pKa = 9.32 PVGLVEE330 pKa = 4.06 PFKK333 pKa = 11.27 VRR335 pKa = 11.84 VITRR339 pKa = 11.84 GAAHH343 pKa = 7.39 HH344 pKa = 5.49 YY345 pKa = 9.71 HH346 pKa = 7.26 LARR349 pKa = 11.84 RR350 pKa = 11.84 WQPSMWRR357 pKa = 11.84 PLAAHH362 pKa = 6.54 PTFQLVGRR370 pKa = 11.84 PCGRR374 pKa = 11.84 DD375 pKa = 3.17 VMYY378 pKa = 10.99 DD379 pKa = 3.29 LVRR382 pKa = 11.84 KK383 pKa = 9.99 VDD385 pKa = 4.08 FGDD388 pKa = 3.24 GRR390 pKa = 11.84 LWMSGDD396 pKa = 3.51 YY397 pKa = 10.04 QAATDD402 pKa = 3.96 YY403 pKa = 10.98 FDD405 pKa = 5.43 PEE407 pKa = 4.39 LSGHH411 pKa = 6.36 CLMEE415 pKa = 4.41 VCDD418 pKa = 4.11 SLGVPWEE425 pKa = 3.86 DD426 pKa = 3.38 RR427 pKa = 11.84 IVLRR431 pKa = 11.84 RR432 pKa = 11.84 ALIGHH437 pKa = 6.29 VFHH440 pKa = 7.84 DD441 pKa = 4.77 PDD443 pKa = 3.3 THH445 pKa = 6.9 EE446 pKa = 4.38 YY447 pKa = 10.5 LGHH450 pKa = 5.19 QARR453 pKa = 11.84 GQLMGSPVSFPILCLLNAALTRR475 pKa = 11.84 FAFEE479 pKa = 3.76 LAGRR483 pKa = 11.84 EE484 pKa = 3.85 ASMLRR489 pKa = 11.84 DD490 pKa = 3.34 QPILVNGDD498 pKa = 3.88 DD499 pKa = 4.15 LLCRR503 pKa = 11.84 TSDD506 pKa = 3.04 KK507 pKa = 10.87 EE508 pKa = 4.09 YY509 pKa = 10.73 YY510 pKa = 9.33 IWSQITAMGGLIPSLGKK527 pKa = 10.18 CFRR530 pKa = 11.84 HH531 pKa = 6.41 RR532 pKa = 11.84 RR533 pKa = 11.84 IATINSEE540 pKa = 3.72 MWVCRR545 pKa = 11.84 KK546 pKa = 8.49 MRR548 pKa = 11.84 TTLLGPLDD556 pKa = 4.31 GPLSEE561 pKa = 4.56 FTYY564 pKa = 8.21 TTIEE568 pKa = 4.07 RR569 pKa = 11.84 RR570 pKa = 11.84 HH571 pKa = 6.06 LPLEE575 pKa = 4.11 GLARR579 pKa = 11.84 GSIKK583 pKa = 10.46 GASGTTDD590 pKa = 2.81 SQRR593 pKa = 11.84 RR594 pKa = 11.84 IEE596 pKa = 5.07 KK597 pKa = 9.78 MSLLDD602 pKa = 3.59 SSNVRR607 pKa = 11.84 SLVPMGDD614 pKa = 2.98 CWRR617 pKa = 11.84 EE618 pKa = 3.97 YY619 pKa = 10.71 VRR621 pKa = 11.84 STPSYY626 pKa = 10.84 ADD628 pKa = 3.22 EE629 pKa = 4.44 SSPGVRR635 pKa = 11.84 AALQRR640 pKa = 11.84 RR641 pKa = 11.84 LWDD644 pKa = 3.27 HH645 pKa = 6.27 CWDD648 pKa = 3.7 VNRR651 pKa = 11.84 DD652 pKa = 3.24 RR653 pKa = 11.84 LKK655 pKa = 10.61 RR656 pKa = 11.84 ISRR659 pKa = 11.84 FPISFCMPVRR669 pKa = 11.84 YY670 pKa = 9.72 GGLGFPLPPPSSRR683 pKa = 11.84 SYY685 pKa = 10.97 AQRR688 pKa = 11.84 LPKK691 pKa = 10.17 ANNWAMANLLANRR704 pKa = 11.84 PNLRR708 pKa = 11.84 RR709 pKa = 11.84 ALLSQLKK716 pKa = 9.98 PDD718 pKa = 3.69 VPCYY722 pKa = 10.97 GDD724 pKa = 2.96 VAYY727 pKa = 9.58 PLRR730 pKa = 11.84 CVPNRR735 pKa = 11.84 DD736 pKa = 3.37 ANLDD740 pKa = 3.58 EE741 pKa = 5.38 EE742 pKa = 4.74 YY743 pKa = 11.17 DD744 pKa = 3.76 SFRR747 pKa = 11.84 RR748 pKa = 11.84 SYY750 pKa = 11.03 PDD752 pKa = 3.61 LPPEE756 pKa = 4.03 SFAPRR761 pKa = 11.84 RR762 pKa = 11.84 GLPSRR767 pKa = 11.84 PSTDD771 pKa = 2.87 PSFWGMCGSEE781 pKa = 3.77 PTQYY785 pKa = 11.35 YY786 pKa = 10.35 EE787 pKa = 4.96 DD788 pKa = 4.34 DD789 pKa = 3.78 SGFLRR794 pKa = 11.84 IWNAANRR801 pKa = 11.84 IAQSKK806 pKa = 9.53 NSSGKK811 pKa = 9.4 YY812 pKa = 7.88 RR813 pKa = 11.84 WAPLRR818 pKa = 11.84 RR819 pKa = 11.84 LSLLDD824 pKa = 3.87 TIALSDD830 pKa = 3.78 HH831 pKa = 6.7 KK832 pKa = 10.73 LTGLDD837 pKa = 4.42 LPHH840 pKa = 7.71 SDD842 pKa = 2.42 IRR844 pKa = 11.84 SPFRR848 pKa = 11.84 DD849 pKa = 3.15 TGVSRR854 pKa = 11.84 HH855 pKa = 5.37 EE856 pKa = 4.01 VAALGDD862 pKa = 3.78 VVLNEE867 pKa = 4.11 LWW869 pKa = 3.59
Molecular weight: 98.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.843
IPC2_protein 7.044
IPC_protein 6.927
Toseland 6.751
ProMoST 7.483
Dawson 7.556
Bjellqvist 7.966
Wikipedia 7.483
Rodwell 7.571
Grimsley 6.839
Solomon 7.571
Lehninger 7.6
Nozaki 8.2
DTASelect 7.775
Thurlkill 7.819
EMBOSS 7.819
Sillero 8.097
Patrickios 4.202
IPC_peptide 7.571
IPC2_peptide 7.468
IPC2.peptide.svr19 7.119
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
869
869
869
869.0
98.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.135 ± 0.0
2.532 ± 0.0
6.214 ± 0.0
5.639 ± 0.0
2.647 ± 0.0
7.595 ± 0.0
2.647 ± 0.0
3.222 ± 0.0
2.647 ± 0.0
10.587 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.417 ± 0.0
2.071 ± 0.0
7.365 ± 0.0
3.222 ± 0.0
9.551 ± 0.0
7.71 ± 0.0
4.603 ± 0.0
5.869 ± 0.0
2.532 ± 0.0
3.797 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here