Natronoarchaeum philippinense

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Natronoarchaeum

Average proteome isoelectric point is 4.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3284 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A285NWS5|A0A285NWS5_9EURY Uncharacterized protein OS=Natronoarchaeum philippinense OX=558529 GN=SAMN06269185_1491 PE=4 SV=1
MM1 pKa = 5.91TTRR4 pKa = 11.84WAEE7 pKa = 3.35WDD9 pKa = 4.22HH10 pKa = 6.22IVKK13 pKa = 9.88IDD15 pKa = 3.66PDD17 pKa = 3.47KK18 pKa = 11.11TLVEE22 pKa = 4.48GEE24 pKa = 4.34TFEE27 pKa = 5.39DD28 pKa = 3.95VCEE31 pKa = 4.24TGTDD35 pKa = 3.65AIEE38 pKa = 4.22IGGTTGMTTDD48 pKa = 2.83KK49 pKa = 9.06MQRR52 pKa = 11.84VIDD55 pKa = 3.67ACATHH60 pKa = 7.15DD61 pKa = 3.69VPLYY65 pKa = 10.3LEE67 pKa = 4.45PSAPEE72 pKa = 3.63VAVNDD77 pKa = 3.99EE78 pKa = 4.45ALDD81 pKa = 4.55GYY83 pKa = 10.5LIPTVLNAGDD93 pKa = 4.08PFWTVGAHH101 pKa = 6.2KK102 pKa = 9.69EE103 pKa = 3.88WARR106 pKa = 11.84MEE108 pKa = 6.03DD109 pKa = 3.82DD110 pKa = 5.06LPWDD114 pKa = 4.93RR115 pKa = 11.84ITTEE119 pKa = 4.51AYY121 pKa = 10.49VVMNPEE127 pKa = 4.11ASVATYY133 pKa = 10.19TEE135 pKa = 4.47ADD137 pKa = 4.04CDD139 pKa = 4.16LSPDD143 pKa = 3.57DD144 pKa = 3.54VAAYY148 pKa = 10.56AEE150 pKa = 4.47VAEE153 pKa = 4.4EE154 pKa = 4.08MFGQEE159 pKa = 3.39IVYY162 pKa = 9.94IEE164 pKa = 4.02YY165 pKa = 10.61SGTLGDD171 pKa = 3.82PAVVEE176 pKa = 4.38AAADD180 pKa = 3.81ATDD183 pKa = 4.3DD184 pKa = 3.6AALFYY189 pKa = 11.19GGGVHH194 pKa = 7.6DD195 pKa = 5.24YY196 pKa = 11.41DD197 pKa = 4.4SAHH200 pKa = 6.21QMGEE204 pKa = 3.87HH205 pKa = 6.79ADD207 pKa = 3.74VVVVGDD213 pKa = 3.91LVHH216 pKa = 7.21AEE218 pKa = 3.96GVDD221 pKa = 3.53AVEE224 pKa = 4.14EE225 pKa = 4.33TVRR228 pKa = 11.84GAKK231 pKa = 9.94DD232 pKa = 2.87AA233 pKa = 4.34

Molecular weight: 25.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A285N1W4|A0A285N1W4_9EURY C2H2-type domain-containing protein OS=Natronoarchaeum philippinense OX=558529 GN=SAMN06269185_0274 PE=4 SV=1
MM1 pKa = 7.45NPTIGVDD8 pKa = 3.34ALSGAEE14 pKa = 4.03GVLGYY19 pKa = 11.44ALVFVLAAIPVVEE32 pKa = 4.19VLVVVPAGVAVGLNPPLVALAAFAGNLATVLLVLAGSDD70 pKa = 2.95RR71 pKa = 11.84ALAFVRR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84FGSADD84 pKa = 3.41DD85 pKa = 4.86DD86 pKa = 4.15EE87 pKa = 4.78PSKK90 pKa = 10.56RR91 pKa = 11.84VRR93 pKa = 11.84RR94 pKa = 11.84AKK96 pKa = 10.38RR97 pKa = 11.84LWRR100 pKa = 11.84QYY102 pKa = 8.39GTAGLALAAPITTGAHH118 pKa = 5.98LAAVLALSLGSKK130 pKa = 9.57RR131 pKa = 11.84RR132 pKa = 11.84AVATWMATSLLAWTAALATVSVYY155 pKa = 10.73GVDD158 pKa = 3.38SLRR161 pKa = 11.84GLFF164 pKa = 3.95

Molecular weight: 16.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3284

0

3284

928822

27

1889

282.8

30.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.236 ± 0.072

0.694 ± 0.014

8.981 ± 0.057

8.701 ± 0.061

3.201 ± 0.027

8.508 ± 0.044

1.975 ± 0.022

4.126 ± 0.03

1.799 ± 0.028

8.791 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.758 ± 0.018

2.292 ± 0.029

4.541 ± 0.026

2.695 ± 0.029

6.477 ± 0.043

5.543 ± 0.038

6.025 ± 0.033

8.838 ± 0.038

1.143 ± 0.018

2.676 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski