Amino acid dipepetide frequency for Nostoc phage A1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.553AlaAla: 3.553 ± 0.512
0.47AlaCys: 0.47 ± 0.159
2.978AlaAsp: 2.978 ± 0.395
3.03AlaGlu: 3.03 ± 0.45
3.71AlaPhe: 3.71 ± 0.489
3.971AlaGly: 3.971 ± 0.476
0.47AlaHis: 0.47 ± 0.157
5.904AlaIle: 5.904 ± 0.444
4.337AlaLys: 4.337 ± 0.502
5.591AlaLeu: 5.591 ± 0.542
1.463AlaMet: 1.463 ± 0.289
3.71AlaAsn: 3.71 ± 0.526
1.829AlaPro: 1.829 ± 0.347
1.881AlaGln: 1.881 ± 0.261
2.508AlaArg: 2.508 ± 0.425
3.605AlaSer: 3.605 ± 0.431
3.814AlaThr: 3.814 ± 0.687
3.135AlaVal: 3.135 ± 0.355
0.784AlaTrp: 0.784 ± 0.173
2.247AlaTyr: 2.247 ± 0.322
0.0AlaXaa: 0.0 ± 0.0
Cys
0.313CysAla: 0.313 ± 0.117
0.157CysCys: 0.157 ± 0.081
0.731CysAsp: 0.731 ± 0.223
0.784CysGlu: 0.784 ± 0.203
0.522CysPhe: 0.522 ± 0.177
0.731CysGly: 0.731 ± 0.181
0.313CysHis: 0.313 ± 0.137
0.993CysIle: 0.993 ± 0.24
1.149CysLys: 1.149 ± 0.285
0.993CysLeu: 0.993 ± 0.251
0.104CysMet: 0.104 ± 0.075
0.47CysAsn: 0.47 ± 0.169
0.627CysPro: 0.627 ± 0.169
0.47CysGln: 0.47 ± 0.157
0.679CysArg: 0.679 ± 0.208
0.575CysSer: 0.575 ± 0.239
0.679CysThr: 0.679 ± 0.151
0.731CysVal: 0.731 ± 0.177
0.313CysTrp: 0.313 ± 0.155
0.679CysTyr: 0.679 ± 0.192
0.0CysXaa: 0.0 ± 0.0
Asp
3.866AspAla: 3.866 ± 0.441
0.366AspCys: 0.366 ± 0.136
3.971AspAsp: 3.971 ± 0.486
3.605AspGlu: 3.605 ± 0.46
3.187AspPhe: 3.187 ± 0.397
3.135AspGly: 3.135 ± 0.441
0.836AspHis: 0.836 ± 0.216
3.135AspIle: 3.135 ± 0.398
4.075AspLys: 4.075 ± 0.441
6.583AspLeu: 6.583 ± 0.622
0.575AspMet: 0.575 ± 0.135
3.239AspAsn: 3.239 ± 0.367
2.821AspPro: 2.821 ± 0.338
2.247AspGln: 2.247 ± 0.334
2.194AspArg: 2.194 ± 0.373
4.284AspSer: 4.284 ± 0.523
3.971AspThr: 3.971 ± 0.437
3.292AspVal: 3.292 ± 0.49
0.784AspTrp: 0.784 ± 0.191
2.247AspTyr: 2.247 ± 0.331
0.0AspXaa: 0.0 ± 0.0
Glu
3.083GluAla: 3.083 ± 0.458
0.993GluCys: 0.993 ± 0.24
2.821GluAsp: 2.821 ± 0.421
3.71GluGlu: 3.71 ± 0.523
2.926GluPhe: 2.926 ± 0.36
2.769GluGly: 2.769 ± 0.363
1.097GluHis: 1.097 ± 0.221
5.016GluIle: 5.016 ± 0.71
5.434GluLys: 5.434 ± 0.558
5.852GluLeu: 5.852 ± 0.619
1.254GluMet: 1.254 ± 0.218
5.277GluAsn: 5.277 ± 0.608
1.933GluPro: 1.933 ± 0.317
2.821GluGln: 2.821 ± 0.338
2.56GluArg: 2.56 ± 0.382
5.434GluSer: 5.434 ± 0.575
3.396GluThr: 3.396 ± 0.453
3.866GluVal: 3.866 ± 0.635
0.993GluTrp: 0.993 ± 0.263
3.03GluTyr: 3.03 ± 0.394
0.0GluXaa: 0.0 ± 0.0
Phe
2.09PheAla: 2.09 ± 0.266
0.627PheCys: 0.627 ± 0.162
3.866PheAsp: 3.866 ± 0.48
3.396PheGlu: 3.396 ± 0.449
2.038PhePhe: 2.038 ± 0.31
2.717PheGly: 2.717 ± 0.589
0.679PheHis: 0.679 ± 0.166
3.083PheIle: 3.083 ± 0.415
3.083PheLys: 3.083 ± 0.408
4.598PheLeu: 4.598 ± 0.544
1.097PheMet: 1.097 ± 0.255
3.239PheAsn: 3.239 ± 0.387
2.038PhePro: 2.038 ± 0.373
1.358PheGln: 1.358 ± 0.276
1.463PheArg: 1.463 ± 0.237
2.717PheSer: 2.717 ± 0.309
3.553PheThr: 3.553 ± 0.444
3.083PheVal: 3.083 ± 0.432
0.47PheTrp: 0.47 ± 0.163
1.985PheTyr: 1.985 ± 0.259
0.0PheXaa: 0.0 ± 0.0
Gly
3.135GlyAla: 3.135 ± 0.462
0.888GlyCys: 0.888 ± 0.225
3.135GlyAsp: 3.135 ± 0.411
3.344GlyGlu: 3.344 ± 0.412
3.448GlyPhe: 3.448 ± 0.49
3.814GlyGly: 3.814 ± 0.598
1.149GlyHis: 1.149 ± 0.322
3.71GlyIle: 3.71 ± 0.505
4.911GlyLys: 4.911 ± 0.676
5.225GlyLeu: 5.225 ± 0.679
1.045GlyMet: 1.045 ± 0.255
3.501GlyAsn: 3.501 ± 0.464
0.0GlyPro: 0.0 ± 0.0
2.09GlyGln: 2.09 ± 0.357
2.508GlyArg: 2.508 ± 0.328
3.762GlySer: 3.762 ± 0.509
4.807GlyThr: 4.807 ± 0.577
5.173GlyVal: 5.173 ± 0.484
0.679GlyTrp: 0.679 ± 0.171
3.292GlyTyr: 3.292 ± 0.322
0.0GlyXaa: 0.0 ± 0.0
His
0.784HisAla: 0.784 ± 0.172
0.575HisCys: 0.575 ± 0.177
0.784HisAsp: 0.784 ± 0.253
0.993HisGlu: 0.993 ± 0.234
0.679HisPhe: 0.679 ± 0.213
1.254HisGly: 1.254 ± 0.335
0.366HisHis: 0.366 ± 0.116
1.097HisIle: 1.097 ± 0.227
0.836HisLys: 0.836 ± 0.21
1.724HisLeu: 1.724 ± 0.291
0.104HisMet: 0.104 ± 0.074
0.522HisAsn: 0.522 ± 0.194
0.784HisPro: 0.784 ± 0.209
0.627HisGln: 0.627 ± 0.199
0.94HisArg: 0.94 ± 0.242
0.94HisSer: 0.94 ± 0.299
0.784HisThr: 0.784 ± 0.197
0.888HisVal: 0.888 ± 0.212
0.47HisTrp: 0.47 ± 0.183
0.784HisTyr: 0.784 ± 0.171
0.0HisXaa: 0.0 ± 0.0
Ile
6.636IleAla: 6.636 ± 0.576
0.627IleCys: 0.627 ± 0.216
6.74IleAsp: 6.74 ± 0.641
6.165IleGlu: 6.165 ± 0.551
2.978IlePhe: 2.978 ± 0.404
4.546IleGly: 4.546 ± 0.515
0.784IleHis: 0.784 ± 0.227
5.329IleIle: 5.329 ± 0.469
5.329IleLys: 5.329 ± 0.622
4.807IleLeu: 4.807 ± 0.486
0.888IleMet: 0.888 ± 0.214
5.747IleAsn: 5.747 ± 0.539
2.142IlePro: 2.142 ± 0.455
2.142IleGln: 2.142 ± 0.328
2.821IleArg: 2.821 ± 0.431
5.068IleSer: 5.068 ± 0.477
3.866IleThr: 3.866 ± 0.495
4.232IleVal: 4.232 ± 0.506
0.522IleTrp: 0.522 ± 0.158
3.083IleTyr: 3.083 ± 0.392
0.0IleXaa: 0.0 ± 0.0
Lys
4.859LysAla: 4.859 ± 0.538
0.47LysCys: 0.47 ± 0.161
3.866LysAsp: 3.866 ± 0.525
4.65LysGlu: 4.65 ± 0.604
2.821LysPhe: 2.821 ± 0.448
2.926LysGly: 2.926 ± 0.348
1.515LysHis: 1.515 ± 0.263
6.27LysIle: 6.27 ± 0.561
6.009LysLys: 6.009 ± 0.698
6.165LysLeu: 6.165 ± 0.734
1.985LysMet: 1.985 ± 0.383
4.493LysAsn: 4.493 ± 0.5
2.978LysPro: 2.978 ± 0.463
3.605LysGln: 3.605 ± 0.453
3.762LysArg: 3.762 ± 0.551
5.016LysSer: 5.016 ± 0.528
4.18LysThr: 4.18 ± 0.476
4.337LysVal: 4.337 ± 0.418
0.575LysTrp: 0.575 ± 0.198
3.083LysTyr: 3.083 ± 0.553
0.0LysXaa: 0.0 ± 0.0
Leu
4.284LeuAla: 4.284 ± 0.509
1.202LeuCys: 1.202 ± 0.226
4.859LeuAsp: 4.859 ± 0.469
6.688LeuGlu: 6.688 ± 0.652
3.919LeuPhe: 3.919 ± 0.44
4.441LeuGly: 4.441 ± 0.512
1.463LeuHis: 1.463 ± 0.35
7.315LeuIle: 7.315 ± 0.739
7.315LeuLys: 7.315 ± 0.816
6.897LeuLeu: 6.897 ± 0.609
1.149LeuMet: 1.149 ± 0.257
5.486LeuAsn: 5.486 ± 0.338
4.023LeuPro: 4.023 ± 0.369
3.971LeuGln: 3.971 ± 0.561
3.344LeuArg: 3.344 ± 0.366
6.74LeuSer: 6.74 ± 0.641
5.956LeuThr: 5.956 ± 0.482
4.18LeuVal: 4.18 ± 0.379
0.784LeuTrp: 0.784 ± 0.196
2.612LeuTyr: 2.612 ± 0.313
0.0LeuXaa: 0.0 ± 0.0
Met
1.045MetAla: 1.045 ± 0.282
0.157MetCys: 0.157 ± 0.097
0.94MetAsp: 0.94 ± 0.215
0.94MetGlu: 0.94 ± 0.204
0.993MetPhe: 0.993 ± 0.214
0.94MetGly: 0.94 ± 0.246
0.209MetHis: 0.209 ± 0.127
1.933MetIle: 1.933 ± 0.366
1.829MetLys: 1.829 ± 0.339
1.724MetLeu: 1.724 ± 0.299
0.784MetMet: 0.784 ± 0.229
1.411MetAsn: 1.411 ± 0.266
0.731MetPro: 0.731 ± 0.184
0.522MetGln: 0.522 ± 0.158
0.731MetArg: 0.731 ± 0.18
1.306MetSer: 1.306 ± 0.276
1.254MetThr: 1.254 ± 0.272
1.776MetVal: 1.776 ± 0.242
0.0MetTrp: 0.0 ± 0.0
0.575MetTyr: 0.575 ± 0.165
0.0MetXaa: 0.0 ± 0.0
Asn
3.814AsnAla: 3.814 ± 0.489
0.836AsnCys: 0.836 ± 0.239
4.441AsnAsp: 4.441 ± 0.481
2.665AsnGlu: 2.665 ± 0.452
2.665AsnPhe: 2.665 ± 0.371
4.755AsnGly: 4.755 ± 0.486
1.149AsnHis: 1.149 ± 0.26
4.389AsnIle: 4.389 ± 0.556
3.762AsnLys: 3.762 ± 0.355
4.075AsnLeu: 4.075 ± 0.395
1.515AsnMet: 1.515 ± 0.258
3.919AsnAsn: 3.919 ± 0.534
2.403AsnPro: 2.403 ± 0.381
2.508AsnGln: 2.508 ± 0.389
3.396AsnArg: 3.396 ± 0.376
4.023AsnSer: 4.023 ± 0.435
3.292AsnThr: 3.292 ± 0.449
4.075AsnVal: 4.075 ± 0.515
0.888AsnTrp: 0.888 ± 0.202
2.612AsnTyr: 2.612 ± 0.399
0.0AsnXaa: 0.0 ± 0.0
Pro
1.985ProAla: 1.985 ± 0.295
0.366ProCys: 0.366 ± 0.129
1.933ProAsp: 1.933 ± 0.343
3.448ProGlu: 3.448 ± 0.467
1.358ProPhe: 1.358 ± 0.231
2.769ProGly: 2.769 ± 0.381
0.522ProHis: 0.522 ± 0.173
2.978ProIle: 2.978 ± 0.44
2.665ProLys: 2.665 ± 0.377
2.56ProLeu: 2.56 ± 0.394
0.94ProMet: 0.94 ± 0.228
1.62ProAsn: 1.62 ± 0.263
1.985ProPro: 1.985 ± 0.352
1.985ProGln: 1.985 ± 0.3
1.724ProArg: 1.724 ± 0.262
1.567ProSer: 1.567 ± 0.288
2.142ProThr: 2.142 ± 0.404
2.612ProVal: 2.612 ± 0.332
0.261ProTrp: 0.261 ± 0.111
1.306ProTyr: 1.306 ± 0.244
0.0ProXaa: 0.0 ± 0.0
Gln
2.247GlnAla: 2.247 ± 0.413
0.418GlnCys: 0.418 ± 0.189
0.836GlnAsp: 0.836 ± 0.187
2.194GlnGlu: 2.194 ± 0.336
1.62GlnPhe: 1.62 ± 0.29
2.299GlnGly: 2.299 ± 0.456
0.94GlnHis: 0.94 ± 0.22
2.821GlnIle: 2.821 ± 0.317
1.985GlnLys: 1.985 ± 0.335
3.866GlnLeu: 3.866 ± 0.563
1.202GlnMet: 1.202 ± 0.22
2.194GlnAsn: 2.194 ± 0.335
1.62GlnPro: 1.62 ± 0.255
1.985GlnGln: 1.985 ± 0.365
1.829GlnArg: 1.829 ± 0.368
3.553GlnSer: 3.553 ± 0.438
1.829GlnThr: 1.829 ± 0.311
2.142GlnVal: 2.142 ± 0.316
0.627GlnTrp: 0.627 ± 0.182
1.881GlnTyr: 1.881 ± 0.288
0.0GlnXaa: 0.0 ± 0.0
Arg
1.463ArgAla: 1.463 ± 0.32
0.679ArgCys: 0.679 ± 0.165
1.985ArgAsp: 1.985 ± 0.329
3.239ArgGlu: 3.239 ± 0.44
2.194ArgPhe: 2.194 ± 0.326
1.672ArgGly: 1.672 ± 0.295
0.993ArgHis: 0.993 ± 0.223
2.351ArgIle: 2.351 ± 0.345
3.762ArgLys: 3.762 ± 0.597
5.12ArgLeu: 5.12 ± 0.58
0.993ArgMet: 0.993 ± 0.225
2.56ArgAsn: 2.56 ± 0.423
1.254ArgPro: 1.254 ± 0.254
1.776ArgGln: 1.776 ± 0.356
1.724ArgArg: 1.724 ± 0.359
2.09ArgSer: 2.09 ± 0.366
2.56ArgThr: 2.56 ± 0.431
3.083ArgVal: 3.083 ± 0.434
0.47ArgTrp: 0.47 ± 0.111
2.403ArgTyr: 2.403 ± 0.431
0.0ArgXaa: 0.0 ± 0.0
Ser
4.546SerAla: 4.546 ± 0.44
0.575SerCys: 0.575 ± 0.144
4.964SerAsp: 4.964 ± 0.452
3.657SerGlu: 3.657 ± 0.493
2.978SerPhe: 2.978 ± 0.393
5.904SerGly: 5.904 ± 0.538
0.888SerHis: 0.888 ± 0.238
4.284SerIle: 4.284 ± 0.55
4.65SerLys: 4.65 ± 0.429
5.382SerLeu: 5.382 ± 0.534
1.097SerMet: 1.097 ± 0.229
4.128SerAsn: 4.128 ± 0.454
2.299SerPro: 2.299 ± 0.328
2.299SerGln: 2.299 ± 0.424
2.194SerArg: 2.194 ± 0.303
4.232SerSer: 4.232 ± 0.571
4.232SerThr: 4.232 ± 0.542
5.225SerVal: 5.225 ± 0.741
0.679SerTrp: 0.679 ± 0.191
2.874SerTyr: 2.874 ± 0.459
0.0SerXaa: 0.0 ± 0.0
Thr
4.65ThrAla: 4.65 ± 0.707
0.47ThrCys: 0.47 ± 0.149
3.187ThrAsp: 3.187 ± 0.38
3.971ThrGlu: 3.971 ± 0.487
3.501ThrPhe: 3.501 ± 0.38
4.598ThrGly: 4.598 ± 0.527
1.045ThrHis: 1.045 ± 0.213
5.904ThrIle: 5.904 ± 0.467
3.448ThrLys: 3.448 ± 0.485
4.284ThrLeu: 4.284 ± 0.394
0.993ThrMet: 0.993 ± 0.263
2.926ThrAsn: 2.926 ± 0.458
3.135ThrPro: 3.135 ± 0.346
1.411ThrGln: 1.411 ± 0.358
2.612ThrArg: 2.612 ± 0.304
3.814ThrSer: 3.814 ± 0.472
4.755ThrThr: 4.755 ± 0.804
5.8ThrVal: 5.8 ± 0.545
0.575ThrTrp: 0.575 ± 0.176
2.56ThrTyr: 2.56 ± 0.368
0.0ThrXaa: 0.0 ± 0.0
Val
3.814ValAla: 3.814 ± 0.537
1.045ValCys: 1.045 ± 0.214
3.501ValAsp: 3.501 ± 0.464
4.128ValGlu: 4.128 ± 0.571
3.396ValPhe: 3.396 ± 0.343
3.605ValGly: 3.605 ± 0.454
0.157ValHis: 0.157 ± 0.116
4.441ValIle: 4.441 ± 0.55
5.12ValLys: 5.12 ± 0.565
6.061ValLeu: 6.061 ± 0.533
1.254ValMet: 1.254 ± 0.224
3.762ValAsn: 3.762 ± 0.515
2.508ValPro: 2.508 ± 0.332
1.776ValGln: 1.776 ± 0.287
3.083ValArg: 3.083 ± 0.397
4.755ValSer: 4.755 ± 0.432
5.538ValThr: 5.538 ± 0.617
5.016ValVal: 5.016 ± 0.486
0.784ValTrp: 0.784 ± 0.209
2.351ValTyr: 2.351 ± 0.351
0.0ValXaa: 0.0 ± 0.0
Trp
0.313TrpAla: 0.313 ± 0.125
0.366TrpCys: 0.366 ± 0.154
0.627TrpAsp: 0.627 ± 0.193
1.358TrpGlu: 1.358 ± 0.247
0.47TrpPhe: 0.47 ± 0.162
0.47TrpGly: 0.47 ± 0.135
0.157TrpHis: 0.157 ± 0.091
0.888TrpIle: 0.888 ± 0.186
0.731TrpLys: 0.731 ± 0.187
0.993TrpLeu: 0.993 ± 0.282
0.313TrpMet: 0.313 ± 0.109
0.731TrpAsn: 0.731 ± 0.176
0.157TrpPro: 0.157 ± 0.083
0.575TrpGln: 0.575 ± 0.162
0.366TrpArg: 0.366 ± 0.118
0.836TrpSer: 0.836 ± 0.192
0.575TrpThr: 0.575 ± 0.169
0.888TrpVal: 0.888 ± 0.175
0.104TrpTrp: 0.104 ± 0.082
0.522TrpTyr: 0.522 ± 0.197
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.508TyrAla: 2.508 ± 0.381
0.731TyrCys: 0.731 ± 0.215
2.247TyrAsp: 2.247 ± 0.339
2.351TyrGlu: 2.351 ± 0.409
1.776TyrPhe: 1.776 ± 0.283
2.299TyrGly: 2.299 ± 0.341
1.202TyrHis: 1.202 ± 0.247
2.612TyrIle: 2.612 ± 0.309
3.135TyrLys: 3.135 ± 0.396
4.128TyrLeu: 4.128 ± 0.458
0.993TyrMet: 0.993 ± 0.266
2.247TyrAsn: 2.247 ± 0.299
1.463TyrPro: 1.463 ± 0.359
2.038TyrGln: 2.038 ± 0.322
1.933TyrArg: 1.933 ± 0.348
2.769TyrSer: 2.769 ± 0.367
2.456TyrThr: 2.456 ± 0.355
2.56TyrVal: 2.56 ± 0.282
0.679TyrTrp: 0.679 ± 0.18
2.351TyrTyr: 2.351 ± 0.335
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (19140 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski