Falsochrobactrum ovis
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3123 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A364JU96|A0A364JU96_9RHIZ Monosaccharide ABC transporter membrane protein (CUT2 family) OS=Falsochrobactrum ovis OX=1293442 GN=C7374_10842 PE=4 SV=1
MM1 pKa = 7.52 GVDD4 pKa = 3.71 QPYY7 pKa = 10.65 LGNFDD12 pKa = 4.1 YY13 pKa = 11.13 DD14 pKa = 3.82 SDD16 pKa = 4.75 LEE18 pKa = 4.36 PPLAAEE24 pKa = 4.33 ATAICATFLSSDD36 pKa = 3.2 AMSGLFIKK44 pKa = 10.43 QMHH47 pKa = 7.13 DD48 pKa = 3.46 GQIKK52 pKa = 9.83
Molecular weight: 5.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.923
IPC_protein 3.783
Toseland 3.592
ProMoST 4.012
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.808
Rodwell 3.63
Grimsley 3.516
Solomon 3.77
Lehninger 3.732
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.681
EMBOSS 3.808
Sillero 3.923
Patrickios 1.926
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|A0A364JTJ3|A0A364JTJ3_9RHIZ Phage tail tube protein OS=Falsochrobactrum ovis OX=1293442 GN=C7374_111100 PE=4 SV=1
MM1 pKa = 7.7 RR2 pKa = 11.84 NFVAVQFSNLQSGAVRR18 pKa = 11.84 TGNGCRR24 pKa = 11.84 WQGWGFPVCNLEE36 pKa = 4.24 HH37 pKa = 6.44 GRR39 pKa = 11.84 SLLAVIRR46 pKa = 11.84 FILSGFRR53 pKa = 11.84 GFASVLQNSPDD64 pKa = 3.36 RR65 pKa = 11.84 VPYY68 pKa = 9.32 MLQSQSPAFVSFQIVPYY85 pKa = 9.78 DD86 pKa = 3.88 LKK88 pKa = 10.84 PSEE91 pKa = 5.47 GFLAWGAKK99 pKa = 9.63 NFSQWNINWLRR110 pKa = 11.84 SAKK113 pKa = 10.22 LL114 pKa = 3.25
Molecular weight: 12.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.692
IPC_protein 10.584
Toseland 10.643
ProMoST 10.394
Dawson 10.76
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.906
Grimsley 10.818
Solomon 10.891
Lehninger 10.862
Nozaki 10.657
DTASelect 10.496
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.701
Patrickios 10.716
IPC_peptide 10.891
IPC2_peptide 9.765
IPC2.peptide.svr19 8.44
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3123
0
3123
950354
29
2860
304.3
33.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.827 ± 0.053
0.799 ± 0.015
5.586 ± 0.037
6.152 ± 0.046
3.976 ± 0.029
7.953 ± 0.052
2.011 ± 0.02
6.436 ± 0.03
4.191 ± 0.035
9.838 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.553 ± 0.02
3.433 ± 0.027
4.678 ± 0.028
3.443 ± 0.028
6.33 ± 0.046
5.986 ± 0.033
5.178 ± 0.032
6.912 ± 0.034
1.248 ± 0.018
2.468 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here