Lactobacillus phage BH1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Behunavirus; Lactobacillus virus BH1

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3LKI7|A0A3G3LKI7_9CAUD Uncharacterized protein OS=Lactobacillus phage BH1 OX=1932007 GN=BVL67_00017 PE=4 SV=1
MM1 pKa = 7.38APEE4 pKa = 3.88KK5 pKa = 10.18RR6 pKa = 11.84VYY8 pKa = 10.55DD9 pKa = 3.78ILSADD14 pKa = 3.43SDD16 pKa = 3.9IADD19 pKa = 3.59KK20 pKa = 11.24VYY22 pKa = 10.66IGTPDD27 pKa = 3.28FNNRR31 pKa = 11.84TSVTPEE37 pKa = 3.62SLAPWVRR44 pKa = 11.84ITSLPGDD51 pKa = 3.82AADD54 pKa = 3.94YY55 pKa = 11.29ADD57 pKa = 4.53DD58 pKa = 4.38SRR60 pKa = 11.84ILEE63 pKa = 4.19YY64 pKa = 10.46PKK66 pKa = 10.4VQVDD70 pKa = 3.79FWVDD74 pKa = 2.97KK75 pKa = 10.85TDD77 pKa = 3.23WDD79 pKa = 3.77QQEE82 pKa = 4.46KK83 pKa = 10.54IEE85 pKa = 4.14TQIYY89 pKa = 8.55QALHH93 pKa = 5.26AAGWEE98 pKa = 3.88RR99 pKa = 11.84YY100 pKa = 8.09YY101 pKa = 11.11RR102 pKa = 11.84NSYY105 pKa = 10.23VDD107 pKa = 3.51GDD109 pKa = 4.18TPALRR114 pKa = 11.84MTTGYY119 pKa = 10.22FQFQGLPIGG128 pKa = 3.96

Molecular weight: 14.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3LKP5|A0A3G3LKP5_9CAUD Uncharacterized protein OS=Lactobacillus phage BH1 OX=1932007 GN=BVL67_00048 PE=4 SV=1
MM1 pKa = 7.36VNVNLDD7 pKa = 3.6RR8 pKa = 11.84LKK10 pKa = 11.05GLMTEE15 pKa = 4.14RR16 pKa = 11.84HH17 pKa = 4.57VTQDD21 pKa = 3.23SLALALGIARR31 pKa = 11.84STLFRR36 pKa = 11.84KK37 pKa = 7.33MQRR40 pKa = 11.84GGKK43 pKa = 9.82DD44 pKa = 3.07FTAQEE49 pKa = 4.32IFKK52 pKa = 9.69MMQFIPLSDD61 pKa = 3.58QEE63 pKa = 5.43AIDD66 pKa = 3.71IFLKK70 pKa = 10.67KK71 pKa = 9.82KK72 pKa = 10.1VAITRR77 pKa = 11.84HH78 pKa = 5.66KK79 pKa = 11.06GMTVV83 pKa = 2.65

Molecular weight: 9.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12421

30

1620

217.9

24.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.22 ± 0.72

0.451 ± 0.097

7.286 ± 0.517

5.547 ± 0.319

3.558 ± 0.187

6.618 ± 0.301

1.868 ± 0.183

5.861 ± 0.196

7.528 ± 0.304

7.817 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.681 ± 0.179

5.241 ± 0.226

3.631 ± 0.231

4.508 ± 0.208

4.5 ± 0.299

6.851 ± 0.369

6.272 ± 0.323

6.296 ± 0.285

1.506 ± 0.17

3.76 ± 0.347
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski