Euhalothece natronophila Z-M001

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Aphanothecaceae; Halothece cluster; Euhalothece; Euhalothece natronophila

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3203 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8NMU6|A0A5B8NMU6_9CHRO Uncharacterized protein OS=Euhalothece natronophila Z-M001 OX=522448 GN=FRE64_11945 PE=4 SV=1
MM1 pKa = 7.8AKK3 pKa = 10.12NALLSTLSASLLLSLTPLPSYY24 pKa = 10.55SSEE27 pKa = 4.07SPEE30 pKa = 3.81CAEE33 pKa = 5.18ASEE36 pKa = 4.84DD37 pKa = 4.78DD38 pKa = 3.5IAALFDD44 pKa = 3.35RR45 pKa = 11.84WNDD48 pKa = 3.23SLATLDD54 pKa = 3.96PDD56 pKa = 3.71QVVDD60 pKa = 4.21NYY62 pKa = 11.54SPDD65 pKa = 3.92AVLLPTLSDD74 pKa = 3.59EE75 pKa = 4.75TRR77 pKa = 11.84DD78 pKa = 3.84TPDD81 pKa = 4.71LIRR84 pKa = 11.84DD85 pKa = 3.72YY86 pKa = 11.03FVDD89 pKa = 5.65FVAQEE94 pKa = 3.92PQGVIDD100 pKa = 4.23EE101 pKa = 4.44RR102 pKa = 11.84NIKK105 pKa = 9.79IGCNAAYY112 pKa = 9.94DD113 pKa = 3.7AGVYY117 pKa = 9.27TFTLVDD123 pKa = 3.42EE124 pKa = 5.11EE125 pKa = 4.69GNEE128 pKa = 4.18TEE130 pKa = 3.93EE131 pKa = 3.87TARR134 pKa = 11.84YY135 pKa = 9.08SFVYY139 pKa = 10.03SYY141 pKa = 11.52HH142 pKa = 6.66DD143 pKa = 3.93GEE145 pKa = 4.37WLIEE149 pKa = 4.05HH150 pKa = 6.37HH151 pKa = 6.95HH152 pKa = 6.4SSLMPEE158 pKa = 4.01

Molecular weight: 17.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8NP67|A0A5B8NP67_9CHRO Uma2 family endonuclease OS=Euhalothece natronophila Z-M001 OX=522448 GN=FRE64_08555 PE=4 SV=1
MM1 pKa = 7.58TKK3 pKa = 9.06RR4 pKa = 11.84TLCGTRR10 pKa = 11.84LKK12 pKa = 10.49QKK14 pKa = 9.88RR15 pKa = 11.84KK16 pKa = 8.5SGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84NGRR29 pKa = 11.84KK30 pKa = 9.3VINARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.93KK38 pKa = 9.79GRR40 pKa = 11.84ARR42 pKa = 11.84LSVV45 pKa = 3.12

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3203

0

3203

971495

29

2847

303.3

34.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.39 ± 0.041

0.947 ± 0.015

4.917 ± 0.048

7.659 ± 0.055

3.983 ± 0.032

6.605 ± 0.038

1.832 ± 0.021

6.601 ± 0.037

4.9 ± 0.049

10.862 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.866 ± 0.017

4.325 ± 0.034

4.642 ± 0.029

5.546 ± 0.046

5.081 ± 0.039

6.325 ± 0.028

5.562 ± 0.029

6.393 ± 0.037

1.451 ± 0.021

3.114 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski