Jiulongibacter sediminis
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3642 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P7BVU8|A0A0P7BVU8_9BACT Uncharacterized protein OS=Jiulongibacter sediminis OX=1605367 GN=AFM12_10375 PE=4 SV=1
MM1 pKa = 7.37 KK2 pKa = 10.73 VNFRR6 pKa = 11.84 LLFLFAWLISLNSKK20 pKa = 10.02 AQFNGSNGDD29 pKa = 3.73 GFDD32 pKa = 3.08 KK33 pKa = 9.05 TTFSGYY39 pKa = 10.55 LDD41 pKa = 3.64 GVEE44 pKa = 4.18 PLLFGGGNGDD54 pKa = 4.12 GFDD57 pKa = 3.5 LKK59 pKa = 11.09 LFNGYY64 pKa = 10.4 LDD66 pKa = 3.45 GTEE69 pKa = 3.89 PVLFGGGNGDD79 pKa = 3.63 GFDD82 pKa = 3.68 RR83 pKa = 11.84 LQFMGFLDD91 pKa = 4.79 GYY93 pKa = 10.47 EE94 pKa = 3.96 LALYY98 pKa = 9.41 TGGNGDD104 pKa = 5.0 GFDD107 pKa = 3.23 QSLFSGYY114 pKa = 10.92 LNGTEE119 pKa = 3.82 PVLFGGGNGDD129 pKa = 3.75 GFDD132 pKa = 5.13 LILFTGYY139 pKa = 10.95 LDD141 pKa = 3.69 GLEE144 pKa = 4.08 PVLFGGGNGDD154 pKa = 5.63 GFDD157 pKa = 3.31 QSNFLGYY164 pKa = 10.58 LDD166 pKa = 4.21 GVEE169 pKa = 4.54 PNLFSGGNGDD179 pKa = 4.11 GFDD182 pKa = 3.68 FGARR186 pKa = 11.84 SAYY189 pKa = 10.25 LSPNGPCVQGTLLTINDD206 pKa = 3.62 NPINSGIYY214 pKa = 9.19 EE215 pKa = 4.13 NQGTISSEE223 pKa = 3.86 GKK225 pKa = 9.86 INSGSNVEE233 pKa = 4.18 FTSNRR238 pKa = 11.84 EE239 pKa = 3.86 IILQPGFSSEE249 pKa = 4.07 NNTVFHH255 pKa = 6.99 AFIGPCNTVPVNLL268 pKa = 4.35
Molecular weight: 28.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.719
IPC_protein 3.719
Toseland 3.49
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.088
Thurlkill 3.554
EMBOSS 3.668
Sillero 3.846
Patrickios 1.036
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|A0A0P7C447|A0A0P7C447_9BACT Serine hydroxymethyltransferase OS=Jiulongibacter sediminis OX=1605367 GN=glyA PE=3 SV=1
MM1 pKa = 7.62 NYY3 pKa = 10.45 SSLLTIHH10 pKa = 6.32 SWFRR14 pKa = 11.84 WAVLISLIITIGRR27 pKa = 11.84 AWFGRR32 pKa = 11.84 RR33 pKa = 11.84 NKK35 pKa = 9.5 LTFTTFDD42 pKa = 2.91 NKK44 pKa = 9.75 LRR46 pKa = 11.84 HH47 pKa = 4.86 MTATICHH54 pKa = 5.77 IQLMLGFWLYY64 pKa = 10.6 FISPIVTYY72 pKa = 9.74 FLNNFGEE79 pKa = 4.46 AVHH82 pKa = 6.26 LRR84 pKa = 11.84 EE85 pKa = 3.69 IRR87 pKa = 11.84 FFGMEE92 pKa = 5.14 HH93 pKa = 5.15 ITVMIFAILVITIGSMKK110 pKa = 10.38 AKK112 pKa = 9.9 RR113 pKa = 11.84 KK114 pKa = 9.16 HH115 pKa = 5.98 ADD117 pKa = 3.39 RR118 pKa = 11.84 EE119 pKa = 4.34 KK120 pKa = 11.03 FNTIYY125 pKa = 10.3 VWYY128 pKa = 10.3 SIGLLLILSSIPWSFLPLTSRR149 pKa = 11.84 PLLRR153 pKa = 11.84 VFF155 pKa = 4.66
Molecular weight: 18.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.955
IPC_protein 10.774
Toseland 10.701
ProMoST 10.452
Dawson 10.833
Bjellqvist 10.57
Wikipedia 11.052
Rodwell 11.052
Grimsley 10.891
Solomon 10.935
Lehninger 10.891
Nozaki 10.687
DTASelect 10.555
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.76
Patrickios 10.789
IPC_peptide 10.935
IPC2_peptide 9.56
IPC2.peptide.svr19 8.288
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3642
0
3642
1288792
38
3806
353.9
39.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.821 ± 0.036
0.789 ± 0.014
5.546 ± 0.034
6.975 ± 0.046
5.161 ± 0.033
7.238 ± 0.042
1.793 ± 0.018
6.904 ± 0.036
6.729 ± 0.043
9.547 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.354 ± 0.022
5.395 ± 0.033
3.861 ± 0.023
3.569 ± 0.022
3.848 ± 0.028
6.744 ± 0.039
5.416 ± 0.053
6.261 ± 0.029
1.215 ± 0.015
3.834 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here