Bifidobacteriaceae bacterium NR021

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; unclassified Bifidobacteriaceae (miscellaneous)

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1369 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A1XE36|A0A3A1XE36_9BIFI Dihydropteroate synthase OS=Bifidobacteriaceae bacterium NR021 OX=2026094 GN=folP PE=4 SV=1
FF1 pKa = 7.12GLSAIIEE8 pKa = 4.23YY9 pKa = 10.15AIPVLMFLYY18 pKa = 9.75PLSIVLILLSLCGRR32 pKa = 11.84LFGNSKK38 pKa = 9.65IVYY41 pKa = 8.66AWTLGFTGVAAVYY54 pKa = 10.55DD55 pKa = 4.22FLNTLPTALRR65 pKa = 11.84NALHH69 pKa = 6.26MNDD72 pKa = 3.81MLVVAQNWLPFSEE85 pKa = 5.93FGMAWLCPAVVGFVVGCVRR104 pKa = 11.84MVLFDD109 pKa = 3.99NKK111 pKa = 10.88DD112 pKa = 3.43MEE114 pKa = 4.64EE115 pKa = 4.23VARR118 pKa = 11.84LAAGPDD124 pKa = 3.62LEE126 pKa = 5.12GSVASDD132 pKa = 4.07DD133 pKa = 5.01DD134 pKa = 4.57YY135 pKa = 11.95SSSDD139 pKa = 3.39AEE141 pKa = 4.33FNEE144 pKa = 4.3PADD147 pKa = 3.83FAVSAKK153 pKa = 9.55STVDD157 pKa = 3.2EE158 pKa = 4.98PNFSEE163 pKa = 4.83QSS165 pKa = 3.22

Molecular weight: 17.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A1XH50|A0A3A1XH50_9BIFI Aldo/keto reductase (Fragment) OS=Bifidobacteriaceae bacterium NR021 OX=2026094 GN=CJI50_04310 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.83MKK15 pKa = 9.27HH16 pKa = 5.56GFRR19 pKa = 11.84VRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1369

0

1369

432169

28

3237

315.7

34.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.559 ± 0.08

1.11 ± 0.025

6.076 ± 0.069

6.072 ± 0.071

3.593 ± 0.041

6.769 ± 0.06

2.122 ± 0.031

6.17 ± 0.068

5.799 ± 0.081

8.741 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.497 ± 0.032

4.762 ± 0.06

3.946 ± 0.044

3.513 ± 0.042

5.078 ± 0.065

7.087 ± 0.065

5.55 ± 0.051

7.478 ± 0.063

1.158 ± 0.022

2.919 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski