Streptococcus phage Javan347

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A8Z4|A0A4D6A8Z4_9CAUD Replication initiation protein OS=Streptococcus phage Javan347 OX=2548117 GN=Javan347_0036 PE=4 SV=1
MM1 pKa = 7.75EE2 pKa = 4.39EE3 pKa = 4.06MKK5 pKa = 10.52RR6 pKa = 11.84DD7 pKa = 3.8FAGKK11 pKa = 10.07LYY13 pKa = 10.77RR14 pKa = 11.84KK15 pKa = 9.3ACEE18 pKa = 3.23IAEE21 pKa = 4.34FYY23 pKa = 10.17EE24 pKa = 4.14EE25 pKa = 4.78QMDD28 pKa = 4.39SEE30 pKa = 4.96DD31 pKa = 3.86DD32 pKa = 3.9NEE34 pKa = 4.17VFDD37 pKa = 5.37IEE39 pKa = 4.3EE40 pKa = 4.56CLVEE44 pKa = 4.38LCQLVFDD51 pKa = 4.17EE52 pKa = 5.17MIFVEE57 pKa = 4.73AAVSMKK63 pKa = 10.23RR64 pKa = 11.84YY65 pKa = 7.75ATLPTDD71 pKa = 3.72NPHH74 pKa = 5.98VMSEE78 pKa = 3.86ARR80 pKa = 11.84KK81 pKa = 9.46EE82 pKa = 3.9LTFF85 pKa = 4.51

Molecular weight: 10.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A7B3|A0A4D6A7B3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan347 OX=2548117 GN=Javan347_0023 PE=4 SV=1
MM1 pKa = 7.74FILKK5 pKa = 10.18HH6 pKa = 4.74GTKK9 pKa = 10.08EE10 pKa = 3.8EE11 pKa = 4.05KK12 pKa = 9.95PYY14 pKa = 10.98LRR16 pKa = 11.84AVTIGVTGIDD26 pKa = 2.86ISFSEE31 pKa = 4.21EE32 pKa = 3.18RR33 pKa = 11.84GAIRR37 pKa = 11.84FVSRR41 pKa = 11.84AVAMQVGKK49 pKa = 10.56ALRR52 pKa = 11.84SFGNFYY58 pKa = 10.66VIQVKK63 pKa = 10.07GG64 pKa = 3.29

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

9262

42

1438

205.8

23.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.554 ± 0.554

0.507 ± 0.106

6.025 ± 0.347

8.152 ± 0.448

4.07 ± 0.222

6.025 ± 0.425

1.306 ± 0.136

7.007 ± 0.277

8.497 ± 0.528

8.745 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.483 ± 0.283

5.906 ± 0.285

2.721 ± 0.227

3.973 ± 0.318

4.2 ± 0.306

6.478 ± 0.376

5.755 ± 0.415

6.187 ± 0.272

1.555 ± 0.155

3.854 ± 0.369
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski