Goodfellowiella sp. AN110305

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Goodfellowiella; unclassified Goodfellowiella

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B2WH84|A0A5B2WH84_9PSEU YbaB/EbfC family DNA-binding protein OS=Goodfellowiella sp. AN110305 OX=1892852 GN=F0L68_37905 PE=4 SV=1
MM1 pKa = 8.05AKK3 pKa = 8.77RR4 pKa = 11.84TWWRR8 pKa = 11.84QWGIVAGGVLVAALAVVPTAGAAPVSPAVPAGAEE42 pKa = 3.69IGQVNFPFSCTMPIIGTQTVNVGLSGTLTSQLGSGQQFYY81 pKa = 11.04LSDD84 pKa = 3.67GAGTLTIPANVVNLIAALGADD105 pKa = 3.71SMSGTVNTLQITATGATPASLDD127 pKa = 3.62AANGTPIVIPSTKK140 pKa = 10.08LVADD144 pKa = 3.8QPVVLPLPADD154 pKa = 3.71GLLTVGPYY162 pKa = 8.8TAGTSGIANLGVGGSTATINLADD185 pKa = 3.73ASGNPVLYY193 pKa = 9.76PLDD196 pKa = 3.97VTCDD200 pKa = 3.4APNPTVSLAAVNIGGPAGLPPAQPSTGARR229 pKa = 11.84AVFGDD234 pKa = 3.85IPLGSLTGSVNLPLSCTVSGLGSQDD259 pKa = 2.6ISGTLTGTFARR270 pKa = 11.84VVPGGSQFSITGGGGSLNYY289 pKa = 9.97SASAVNAILAQYY301 pKa = 7.49PTATTASGSLTDD313 pKa = 5.7FEE315 pKa = 4.65VQADD319 pKa = 3.89NATPASVNAASPAIAVGQTALTAGQAASVPIPSGTDD355 pKa = 2.93TLTIGPFTAGPSGGQVTLSAGSSGGTITVQDD386 pKa = 3.65AGGTTLGSAQISCATPNPRR405 pKa = 11.84VALSQISISS414 pKa = 3.48

Molecular weight: 39.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B2XQ60|A0A5B2XQ60_9PSEU HlyC/CorC family transporter OS=Goodfellowiella sp. AN110305 OX=1892852 GN=F0L68_02820 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.46LLRR22 pKa = 11.84KK23 pKa = 7.95TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.84RR31 pKa = 11.84GKK33 pKa = 9.61

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8101

0

8101

2682959

24

7197

331.2

35.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.606 ± 0.043

0.814 ± 0.008

6.094 ± 0.025

5.1 ± 0.027

2.782 ± 0.014

9.242 ± 0.029

2.278 ± 0.016

3.204 ± 0.016

1.791 ± 0.02

10.773 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.612 ± 0.011

2.005 ± 0.019

5.897 ± 0.029

3.014 ± 0.016

7.958 ± 0.032

5.197 ± 0.022

6.164 ± 0.034

8.972 ± 0.026

1.536 ± 0.011

1.961 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski