Cronobacter phage vB_CsaM_GAP161

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Pseudotevenvirus; Cronobacter virus GAP161

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 275 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|K4FBH2|K4FBH2_9CAUD Uncharacterized protein OS=Cronobacter phage vB_CsaM_GAP161 OX=1141138 GN=GAP161_229 PE=4 SV=1
MM1 pKa = 8.26AEE3 pKa = 4.54EE4 pKa = 4.63FDD6 pKa = 4.48CKK8 pKa = 10.58CVVCKK13 pKa = 10.6QPMNSRR19 pKa = 11.84NTAFVNDD26 pKa = 4.64AGPICGVVCLQYY38 pKa = 11.27LNEE41 pKa = 4.26RR42 pKa = 11.84NDD44 pKa = 3.53RR45 pKa = 11.84GINEE49 pKa = 4.49ADD51 pKa = 4.34DD52 pKa = 4.32LNEE55 pKa = 3.95VQMLLL60 pKa = 3.89

Molecular weight:
6.74 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|K4F7J4|K4F7J4_9CAUD DNA primase subunit OS=Cronobacter phage vB_CsaM_GAP161 OX=1141138 GN=GAP161_026 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84LCLVGVGFGRR12 pKa = 11.84ALTQGLIDD20 pKa = 4.4SIRR23 pKa = 11.84WIQPRR28 pKa = 11.84VEE30 pKa = 4.65LYY32 pKa = 11.26AMPNRR37 pKa = 11.84IEE39 pKa = 4.31YY40 pKa = 10.51SDD42 pKa = 3.49PTPYY46 pKa = 10.86KK47 pKa = 9.05MKK49 pKa = 9.91PYY51 pKa = 9.7QAPRR55 pKa = 11.84VNRR58 pKa = 11.84VLHH61 pKa = 5.45RR62 pKa = 11.84QSVNRR67 pKa = 11.84GKK69 pKa = 10.66

Molecular weight:
8.04 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

275

0

275

56770

38

1219

206.4

23.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.074 ± 0.183

1.163 ± 0.067

6.599 ± 0.114

6.865 ± 0.17

4.125 ± 0.109

6.32 ± 0.18

1.874 ± 0.081

6.488 ± 0.114

7.444 ± 0.212

7.596 ± 0.122

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.855 ± 0.09

5.418 ± 0.118

3.643 ± 0.087

3.403 ± 0.09

4.8 ± 0.129

5.75 ± 0.116

5.892 ± 0.228

7.111 ± 0.123

1.328 ± 0.058

4.252 ± 0.1

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski