Cronobacter phage vB_CsaM_GAP161
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 275 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K4FBH2|K4FBH2_9CAUD Uncharacterized protein OS=Cronobacter phage vB_CsaM_GAP161 OX=1141138 GN=GAP161_229 PE=4 SV=1
MM1 pKa = 8.26 AEE3 pKa = 4.54 EE4 pKa = 4.63 FDD6 pKa = 4.48 CKK8 pKa = 10.58 CVVCKK13 pKa = 10.6 QPMNSRR19 pKa = 11.84 NTAFVNDD26 pKa = 4.64 AGPICGVVCLQYY38 pKa = 11.27 LNEE41 pKa = 4.26 RR42 pKa = 11.84 NDD44 pKa = 3.53 RR45 pKa = 11.84 GINEE49 pKa = 4.49 ADD51 pKa = 4.34 DD52 pKa = 4.32 LNEE55 pKa = 3.95 VQMLLL60 pKa = 3.89
Molecular weight: 6.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.974
IPC2_protein 4.329
IPC_protein 4.151
Toseland 3.973
ProMoST 4.291
Dawson 4.113
Bjellqvist 4.279
Wikipedia 4.037
Rodwell 3.986
Grimsley 3.884
Solomon 4.101
Lehninger 4.062
Nozaki 4.253
DTASelect 4.406
Thurlkill 4.024
EMBOSS 4.05
Sillero 4.266
Patrickios 1.964
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.169
Protein with the highest isoelectric point:
>tr|K4F7J4|K4F7J4_9CAUD DNA primase subunit OS=Cronobacter phage vB_CsaM_GAP161 OX=1141138 GN=GAP161_026 PE=4 SV=1
MM1 pKa = 7.75 RR2 pKa = 11.84 LCLVGVGFGRR12 pKa = 11.84 ALTQGLIDD20 pKa = 4.4 SIRR23 pKa = 11.84 WIQPRR28 pKa = 11.84 VEE30 pKa = 4.65 LYY32 pKa = 11.26 AMPNRR37 pKa = 11.84 IEE39 pKa = 4.31 YY40 pKa = 10.51 SDD42 pKa = 3.49 PTPYY46 pKa = 10.86 KK47 pKa = 9.05 MKK49 pKa = 9.91 PYY51 pKa = 9.7 QAPRR55 pKa = 11.84 VNRR58 pKa = 11.84 VLHH61 pKa = 5.45 RR62 pKa = 11.84 QSVNRR67 pKa = 11.84 GKK69 pKa = 10.66
Molecular weight: 8.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.999
IPC_protein 10.906
Toseland 10.687
ProMoST 10.643
Dawson 10.833
Bjellqvist 10.628
Wikipedia 11.111
Rodwell 10.921
Grimsley 10.906
Solomon 10.979
Lehninger 10.935
Nozaki 10.672
DTASelect 10.628
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.774
Patrickios 10.672
IPC_peptide 10.979
IPC2_peptide 9.78
IPC2.peptide.svr19 8.186
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
275
0
275
56770
38
1219
206.4
23.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.074 ± 0.183
1.163 ± 0.067
6.599 ± 0.114
6.865 ± 0.17
4.125 ± 0.109
6.32 ± 0.18
1.874 ± 0.081
6.488 ± 0.114
7.444 ± 0.212
7.596 ± 0.122
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.855 ± 0.09
5.418 ± 0.118
3.643 ± 0.087
3.403 ± 0.09
4.8 ± 0.129
5.75 ± 0.116
5.892 ± 0.228
7.111 ± 0.123
1.328 ± 0.058
4.252 ± 0.1
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here