Sphingomonas sp. Leaf33

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2982 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4KGB0|A0A0Q4KGB0_9SPHN Peptide chain release factor I OS=Sphingomonas sp. Leaf33 OX=1736215 GN=ASE86_05760 PE=3 SV=1
MM1 pKa = 6.97STTRR5 pKa = 11.84HH6 pKa = 5.89IYY8 pKa = 10.87NNTGVAWQIWLDD20 pKa = 3.71GGNLQDD26 pKa = 5.91LPGTPPGEE34 pKa = 4.24GVTSGTLPGKK44 pKa = 5.78TTCAMEE50 pKa = 4.18LVSPNGSIGLKK61 pKa = 10.15SEE63 pKa = 4.2ASGNQWNYY71 pKa = 10.57GYY73 pKa = 8.73STGEE77 pKa = 3.91FSTTPSWDD85 pKa = 3.33HH86 pKa = 6.75NGDD89 pKa = 3.71TNGCSLNDD97 pKa = 3.8PADD100 pKa = 3.76CDD102 pKa = 3.85FQINQDD108 pKa = 3.47VPQQ111 pKa = 4.62

Molecular weight: 11.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4KBR7|A0A0Q4KBR7_9SPHN Uncharacterized protein OS=Sphingomonas sp. Leaf33 OX=1736215 GN=ASE86_12630 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2982

0

2982

943339

29

2301

316.3

33.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.133 ± 0.065

0.692 ± 0.013

6.371 ± 0.038

4.739 ± 0.038

3.363 ± 0.027

9.082 ± 0.046

1.841 ± 0.022

4.815 ± 0.027

2.656 ± 0.036

9.715 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.024

2.306 ± 0.033

5.438 ± 0.037

2.838 ± 0.026

7.741 ± 0.046

4.828 ± 0.035

5.873 ± 0.042

7.719 ± 0.032

1.429 ± 0.021

2.121 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski