Streptomyces uncialis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6725 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q4V127|A0A1Q4V127_9ACTN Uncharacterized protein OS=Streptomyces uncialis OX=1048205 GN=AB852_28680 PE=4 SV=1
MM1 pKa = 7.79PSLNALLDD9 pKa = 3.7NFNDD13 pKa = 4.24GALGPEE19 pKa = 3.99WGNSYY24 pKa = 11.22GGTTEE29 pKa = 3.65TGGRR33 pKa = 11.84ARR35 pKa = 11.84VPCTTGYY42 pKa = 10.71AGCQTAYY49 pKa = 10.3SWTLAGAALYY59 pKa = 9.68VQVPTVPVASTATEE73 pKa = 4.75AYY75 pKa = 8.65CGVMIQSVTAGTRR88 pKa = 11.84AGFIINAVTGLLRR101 pKa = 11.84CKK103 pKa = 10.6SEE105 pKa = 3.66TGYY108 pKa = 11.2YY109 pKa = 10.65DD110 pKa = 4.23PDD112 pKa = 4.04SVDD115 pKa = 2.87ITYY118 pKa = 10.77SAVDD122 pKa = 3.6HH123 pKa = 6.19RR124 pKa = 11.84WLRR127 pKa = 11.84IRR129 pKa = 11.84EE130 pKa = 4.35DD131 pKa = 3.36GTNVYY136 pKa = 9.9WDD138 pKa = 3.87TSPDD142 pKa = 3.11GSTWTTRR149 pKa = 11.84RR150 pKa = 11.84TLATPAWVLTEE161 pKa = 4.01TDD163 pKa = 3.96ALALDD168 pKa = 4.23MSSHH172 pKa = 6.8RR173 pKa = 11.84DD174 pKa = 3.35AGTDD178 pKa = 3.29DD179 pKa = 3.71YY180 pKa = 12.24AEE182 pKa = 4.21YY183 pKa = 11.14DD184 pKa = 4.07CVNTLADD191 pKa = 3.83GAVWDD196 pKa = 4.5ADD198 pKa = 3.42AALGAEE204 pKa = 4.6GGLTAAAAPVTVTSTDD220 pKa = 3.23LTADD224 pKa = 3.94GSLSTPDD231 pKa = 3.7PAITAMAEE239 pKa = 3.88AGLGAEE245 pKa = 4.46SDD247 pKa = 3.79LTAEE251 pKa = 4.72SVGQDD256 pKa = 2.75RR257 pKa = 11.84TDD259 pKa = 2.81IDD261 pKa = 3.87FAIGQPTPGWTVSAPWKK278 pKa = 10.33

Molecular weight: 29.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q4V778|A0A1Q4V778_9ACTN Peptidase U61 OS=Streptomyces uncialis OX=1048205 GN=AB852_19695 PE=3 SV=1
MM1 pKa = 7.55IVPAMARR8 pKa = 11.84VTLVRR13 pKa = 11.84STGVALVRR21 pKa = 11.84SLIRR25 pKa = 11.84TAVPMRR31 pKa = 11.84VLAVFLRR38 pKa = 11.84HH39 pKa = 5.87GSWNRR44 pKa = 11.84LPYY47 pKa = 10.45GRR49 pKa = 11.84GGGGPRR55 pKa = 11.84SGPPRR60 pKa = 11.84TASPRR65 pKa = 11.84VRR67 pKa = 11.84PGGRR71 pKa = 11.84HH72 pKa = 4.23

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6724

1

6725

2184566

29

6109

324.8

34.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.575 ± 0.043

0.754 ± 0.008

6.14 ± 0.022

5.506 ± 0.029

2.659 ± 0.018

9.833 ± 0.035

2.28 ± 0.015

3.004 ± 0.02

1.944 ± 0.025

10.361 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.704 ± 0.013

1.613 ± 0.017

6.385 ± 0.031

2.53 ± 0.017

8.426 ± 0.031

4.956 ± 0.019

6.307 ± 0.025

8.538 ± 0.025

1.497 ± 0.013

1.988 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski