Binucleate Rhizoctonia mitovirus K1
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M4K4R4|A0A0M4K4R4_9VIRU ORF OS=Binucleate Rhizoctonia mitovirus K1 OX=1712387 PE=4 SV=1
MM1 pKa = 6.41 MTLTILKK8 pKa = 10.08 ALRR11 pKa = 11.84 AQSKK15 pKa = 9.2 TIFNVHH21 pKa = 6.85 AYY23 pKa = 10.04 LGVIMRR29 pKa = 11.84 DD30 pKa = 3.18 YY31 pKa = 10.66 STTVTQNRR39 pKa = 11.84 NNQLILDD46 pKa = 4.87 GYY48 pKa = 10.34 KK49 pKa = 10.11 VPKK52 pKa = 10.62 LNIQRR57 pKa = 11.84 NQSRR61 pKa = 11.84 LTKK64 pKa = 10.42 AINALKK70 pKa = 10.51 LYY72 pKa = 10.13 IDD74 pKa = 3.84 NQCPGLEE81 pKa = 3.93 NNVIQEE87 pKa = 4.0 LMVILSHH94 pKa = 7.5 LEE96 pKa = 4.06 TIYY99 pKa = 11.19 HH100 pKa = 7.0 KK101 pKa = 10.6 DD102 pKa = 3.04 PKK104 pKa = 11.16 SFVTQCKK111 pKa = 9.97 LLSNWFKK118 pKa = 11.64 VNVFKK123 pKa = 10.92 QIRR126 pKa = 11.84 RR127 pKa = 11.84 QDD129 pKa = 4.31 AIPLLFWNRR138 pKa = 11.84 RR139 pKa = 11.84 NQAPHH144 pKa = 6.21 ILAPIFGILDD154 pKa = 3.6 APGTVQNHH162 pKa = 5.79 LLVLRR167 pKa = 11.84 LIFSILEE174 pKa = 3.88 IYY176 pKa = 10.68 NVVTVPVLPSSEE188 pKa = 4.43 TITKK192 pKa = 9.67 EE193 pKa = 3.72 FSGDD197 pKa = 2.95 MNMIKK202 pKa = 10.2 SINIGRR208 pKa = 11.84 ALEE211 pKa = 4.0 RR212 pKa = 11.84 LNVNIDD218 pKa = 2.67 SWRR221 pKa = 11.84 QSFEE225 pKa = 3.46 LRR227 pKa = 11.84 NRR229 pKa = 11.84 EE230 pKa = 4.5 GIWHH234 pKa = 6.7 TSSSSGPNGQALWMSHH250 pKa = 5.41 VDD252 pKa = 3.88 AKK254 pKa = 11.3 ALASDD259 pKa = 4.39 KK260 pKa = 11.11 EE261 pKa = 4.22 LMSNFRR267 pKa = 11.84 NYY269 pKa = 7.57 VTLVGRR275 pKa = 11.84 EE276 pKa = 3.83 DD277 pKa = 4.32 LIEE280 pKa = 4.25 NLEE283 pKa = 4.17 DD284 pKa = 3.72 TVRR287 pKa = 11.84 LPQFFKK293 pKa = 10.79 LSSGIPTHH301 pKa = 6.72 SKK303 pKa = 9.59 LHH305 pKa = 5.84 VIFEE309 pKa = 4.55 KK310 pKa = 10.71 GSKK313 pKa = 10.11 ARR315 pKa = 11.84 IIAIGDD321 pKa = 3.46 YY322 pKa = 7.33 WTQEE326 pKa = 3.93 ALTPLHH332 pKa = 6.37 TEE334 pKa = 3.82 LAKK337 pKa = 10.61 ILGGLDD343 pKa = 2.99 MDD345 pKa = 4.28 GTFNQDD351 pKa = 2.89 KK352 pKa = 10.12 IAEE355 pKa = 4.31 KK356 pKa = 9.87 VRR358 pKa = 11.84 RR359 pKa = 11.84 WTAIDD364 pKa = 3.27 NKK366 pKa = 10.62 AIYY369 pKa = 10.64 SLDD372 pKa = 3.22 LSAATDD378 pKa = 3.78 RR379 pKa = 11.84 LPVLLQAMILDD390 pKa = 4.24 HH391 pKa = 6.26 LTGIKK396 pKa = 10.58 GFGDD400 pKa = 3.51 AWRR403 pKa = 11.84 SLVVGRR409 pKa = 11.84 DD410 pKa = 3.71 FTSEE414 pKa = 3.53 IFPKK418 pKa = 10.17 VRR420 pKa = 11.84 YY421 pKa = 8.29 STGQPMGFKK430 pKa = 10.47 SSFVMLGLTHH440 pKa = 6.96 HH441 pKa = 7.11 IIVQEE446 pKa = 3.64 AAARR450 pKa = 11.84 VGFDD454 pKa = 2.86 SFEE457 pKa = 5.21 DD458 pKa = 4.15 YY459 pKa = 11.18 VILGDD464 pKa = 5.05 DD465 pKa = 3.0 IVVANTEE472 pKa = 3.7 VAEE475 pKa = 4.07 QYY477 pKa = 10.62 KK478 pKa = 10.44 IIMEE482 pKa = 4.23 ALGLEE487 pKa = 3.93 ISPFKK492 pKa = 10.63 TIACDD497 pKa = 3.46 ADD499 pKa = 4.04 FSKK502 pKa = 10.92 PIAEE506 pKa = 3.62 ICKK509 pKa = 9.62 RR510 pKa = 11.84 VFVGGKK516 pKa = 9.27 EE517 pKa = 3.96 ISPLPMKK524 pKa = 10.89 LMVTAAEE531 pKa = 4.35 TTDD534 pKa = 3.33 MLYY537 pKa = 10.25 QLQEE541 pKa = 4.11 EE542 pKa = 4.45 MSKK545 pKa = 10.73 RR546 pKa = 11.84 SLINDD551 pKa = 4.31 PLNIHH556 pKa = 6.15 YY557 pKa = 10.46 FMGGISNRR565 pKa = 11.84 VAYY568 pKa = 9.73 KK569 pKa = 10.65 DD570 pKa = 3.5 LARR573 pKa = 11.84 MNGLPMFMTGLKK585 pKa = 10.5 NPILPPILDD594 pKa = 3.32 QFNYY598 pKa = 10.22 KK599 pKa = 9.98 NWDD602 pKa = 3.18 KK603 pKa = 10.91 TRR605 pKa = 11.84 NISLLTLQSYY615 pKa = 7.3 FTFTVMMEE623 pKa = 3.89 QLKK626 pKa = 10.6 RR627 pKa = 11.84 LGDD630 pKa = 3.42 ILVNTKK636 pKa = 10.48 DD637 pKa = 3.36 IYY639 pKa = 7.8 TTLMKK644 pKa = 10.37 AANVSKK650 pKa = 9.67 TVGIASKK657 pKa = 9.57 MDD659 pKa = 3.09 QYY661 pKa = 11.75 LPVEE665 pKa = 4.75 DD666 pKa = 5.66 FSQEE670 pKa = 4.05 ILDD673 pKa = 3.63 EE674 pKa = 4.32 WEE676 pKa = 4.26 VIKK679 pKa = 10.37 PFHH682 pKa = 6.47 PAIEE686 pKa = 4.38 VVKK689 pKa = 11.18 GEE691 pKa = 3.89 VDD693 pKa = 4.94 RR694 pKa = 11.84 INTLLNRR701 pKa = 11.84 ISMASSMDD709 pKa = 3.66 MMVMLLDD716 pKa = 3.93 EE717 pKa = 4.89 VVDD720 pKa = 3.86 SLKK723 pKa = 10.49 ISSFEE728 pKa = 3.85 YY729 pKa = 11.11 VPDD732 pKa = 3.77 KK733 pKa = 10.87 EE734 pKa = 4.19 YY735 pKa = 11.54 AEE737 pKa = 4.65 AKK739 pKa = 5.88 QTRR742 pKa = 11.84 RR743 pKa = 11.84 LVEE746 pKa = 3.82 QTLSNIQRR754 pKa = 11.84 STINGTLGKK763 pKa = 8.86 RR764 pKa = 11.84 TEE766 pKa = 3.93 LSYY769 pKa = 10.81 SIKK772 pKa = 10.56 LEE774 pKa = 4.37 RR775 pKa = 11.84 INAIWNLKK783 pKa = 9.62 VDD785 pKa = 4.48 LDD787 pKa = 4.23 SPISINRR794 pKa = 11.84 NISSISSSNAQAIQRR809 pKa = 11.84 VNAYY813 pKa = 10.05 LSEE816 pKa = 4.08 GVFDD820 pKa = 6.36 DD821 pKa = 3.81 FLKK824 pKa = 11.14 DD825 pKa = 2.98 KK826 pKa = 10.86 GRR828 pKa = 11.84 RR829 pKa = 3.55
Molecular weight: 94.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.091
IPC2_protein 7.527
IPC_protein 7.483
Toseland 7.614
ProMoST 8.126
Dawson 8.258
Bjellqvist 8.302
Wikipedia 8.244
Rodwell 8.317
Grimsley 7.688
Solomon 8.375
Lehninger 8.375
Nozaki 8.404
DTASelect 8.229
Thurlkill 8.317
EMBOSS 8.478
Sillero 8.521
Patrickios 4.66
IPC_peptide 8.375
IPC2_peptide 7.19
IPC2.peptide.svr19 7.499
Protein with the highest isoelectric point:
>tr|A0A0M4K4R4|A0A0M4K4R4_9VIRU ORF OS=Binucleate Rhizoctonia mitovirus K1 OX=1712387 PE=4 SV=1
MM1 pKa = 6.41 MTLTILKK8 pKa = 10.08 ALRR11 pKa = 11.84 AQSKK15 pKa = 9.2 TIFNVHH21 pKa = 6.85 AYY23 pKa = 10.04 LGVIMRR29 pKa = 11.84 DD30 pKa = 3.18 YY31 pKa = 10.66 STTVTQNRR39 pKa = 11.84 NNQLILDD46 pKa = 4.87 GYY48 pKa = 10.34 KK49 pKa = 10.11 VPKK52 pKa = 10.62 LNIQRR57 pKa = 11.84 NQSRR61 pKa = 11.84 LTKK64 pKa = 10.42 AINALKK70 pKa = 10.51 LYY72 pKa = 10.13 IDD74 pKa = 3.84 NQCPGLEE81 pKa = 3.93 NNVIQEE87 pKa = 4.0 LMVILSHH94 pKa = 7.5 LEE96 pKa = 4.06 TIYY99 pKa = 11.19 HH100 pKa = 7.0 KK101 pKa = 10.6 DD102 pKa = 3.04 PKK104 pKa = 11.16 SFVTQCKK111 pKa = 9.97 LLSNWFKK118 pKa = 11.64 VNVFKK123 pKa = 10.92 QIRR126 pKa = 11.84 RR127 pKa = 11.84 QDD129 pKa = 4.31 AIPLLFWNRR138 pKa = 11.84 RR139 pKa = 11.84 NQAPHH144 pKa = 6.21 ILAPIFGILDD154 pKa = 3.6 APGTVQNHH162 pKa = 5.79 LLVLRR167 pKa = 11.84 LIFSILEE174 pKa = 3.88 IYY176 pKa = 10.68 NVVTVPVLPSSEE188 pKa = 4.43 TITKK192 pKa = 9.67 EE193 pKa = 3.72 FSGDD197 pKa = 2.95 MNMIKK202 pKa = 10.2 SINIGRR208 pKa = 11.84 ALEE211 pKa = 4.0 RR212 pKa = 11.84 LNVNIDD218 pKa = 2.67 SWRR221 pKa = 11.84 QSFEE225 pKa = 3.46 LRR227 pKa = 11.84 NRR229 pKa = 11.84 EE230 pKa = 4.5 GIWHH234 pKa = 6.7 TSSSSGPNGQALWMSHH250 pKa = 5.41 VDD252 pKa = 3.88 AKK254 pKa = 11.3 ALASDD259 pKa = 4.39 KK260 pKa = 11.11 EE261 pKa = 4.22 LMSNFRR267 pKa = 11.84 NYY269 pKa = 7.57 VTLVGRR275 pKa = 11.84 EE276 pKa = 3.83 DD277 pKa = 4.32 LIEE280 pKa = 4.25 NLEE283 pKa = 4.17 DD284 pKa = 3.72 TVRR287 pKa = 11.84 LPQFFKK293 pKa = 10.79 LSSGIPTHH301 pKa = 6.72 SKK303 pKa = 9.59 LHH305 pKa = 5.84 VIFEE309 pKa = 4.55 KK310 pKa = 10.71 GSKK313 pKa = 10.11 ARR315 pKa = 11.84 IIAIGDD321 pKa = 3.46 YY322 pKa = 7.33 WTQEE326 pKa = 3.93 ALTPLHH332 pKa = 6.37 TEE334 pKa = 3.82 LAKK337 pKa = 10.61 ILGGLDD343 pKa = 2.99 MDD345 pKa = 4.28 GTFNQDD351 pKa = 2.89 KK352 pKa = 10.12 IAEE355 pKa = 4.31 KK356 pKa = 9.87 VRR358 pKa = 11.84 RR359 pKa = 11.84 WTAIDD364 pKa = 3.27 NKK366 pKa = 10.62 AIYY369 pKa = 10.64 SLDD372 pKa = 3.22 LSAATDD378 pKa = 3.78 RR379 pKa = 11.84 LPVLLQAMILDD390 pKa = 4.24 HH391 pKa = 6.26 LTGIKK396 pKa = 10.58 GFGDD400 pKa = 3.51 AWRR403 pKa = 11.84 SLVVGRR409 pKa = 11.84 DD410 pKa = 3.71 FTSEE414 pKa = 3.53 IFPKK418 pKa = 10.17 VRR420 pKa = 11.84 YY421 pKa = 8.29 STGQPMGFKK430 pKa = 10.47 SSFVMLGLTHH440 pKa = 6.96 HH441 pKa = 7.11 IIVQEE446 pKa = 3.64 AAARR450 pKa = 11.84 VGFDD454 pKa = 2.86 SFEE457 pKa = 5.21 DD458 pKa = 4.15 YY459 pKa = 11.18 VILGDD464 pKa = 5.05 DD465 pKa = 3.0 IVVANTEE472 pKa = 3.7 VAEE475 pKa = 4.07 QYY477 pKa = 10.62 KK478 pKa = 10.44 IIMEE482 pKa = 4.23 ALGLEE487 pKa = 3.93 ISPFKK492 pKa = 10.63 TIACDD497 pKa = 3.46 ADD499 pKa = 4.04 FSKK502 pKa = 10.92 PIAEE506 pKa = 3.62 ICKK509 pKa = 9.62 RR510 pKa = 11.84 VFVGGKK516 pKa = 9.27 EE517 pKa = 3.96 ISPLPMKK524 pKa = 10.89 LMVTAAEE531 pKa = 4.35 TTDD534 pKa = 3.33 MLYY537 pKa = 10.25 QLQEE541 pKa = 4.11 EE542 pKa = 4.45 MSKK545 pKa = 10.73 RR546 pKa = 11.84 SLINDD551 pKa = 4.31 PLNIHH556 pKa = 6.15 YY557 pKa = 10.46 FMGGISNRR565 pKa = 11.84 VAYY568 pKa = 9.73 KK569 pKa = 10.65 DD570 pKa = 3.5 LARR573 pKa = 11.84 MNGLPMFMTGLKK585 pKa = 10.5 NPILPPILDD594 pKa = 3.32 QFNYY598 pKa = 10.22 KK599 pKa = 9.98 NWDD602 pKa = 3.18 KK603 pKa = 10.91 TRR605 pKa = 11.84 NISLLTLQSYY615 pKa = 7.3 FTFTVMMEE623 pKa = 3.89 QLKK626 pKa = 10.6 RR627 pKa = 11.84 LGDD630 pKa = 3.42 ILVNTKK636 pKa = 10.48 DD637 pKa = 3.36 IYY639 pKa = 7.8 TTLMKK644 pKa = 10.37 AANVSKK650 pKa = 9.67 TVGIASKK657 pKa = 9.57 MDD659 pKa = 3.09 QYY661 pKa = 11.75 LPVEE665 pKa = 4.75 DD666 pKa = 5.66 FSQEE670 pKa = 4.05 ILDD673 pKa = 3.63 EE674 pKa = 4.32 WEE676 pKa = 4.26 VIKK679 pKa = 10.37 PFHH682 pKa = 6.47 PAIEE686 pKa = 4.38 VVKK689 pKa = 11.18 GEE691 pKa = 3.89 VDD693 pKa = 4.94 RR694 pKa = 11.84 INTLLNRR701 pKa = 11.84 ISMASSMDD709 pKa = 3.66 MMVMLLDD716 pKa = 3.93 EE717 pKa = 4.89 VVDD720 pKa = 3.86 SLKK723 pKa = 10.49 ISSFEE728 pKa = 3.85 YY729 pKa = 11.11 VPDD732 pKa = 3.77 KK733 pKa = 10.87 EE734 pKa = 4.19 YY735 pKa = 11.54 AEE737 pKa = 4.65 AKK739 pKa = 5.88 QTRR742 pKa = 11.84 RR743 pKa = 11.84 LVEE746 pKa = 3.82 QTLSNIQRR754 pKa = 11.84 STINGTLGKK763 pKa = 8.86 RR764 pKa = 11.84 TEE766 pKa = 3.93 LSYY769 pKa = 10.81 SIKK772 pKa = 10.56 LEE774 pKa = 4.37 RR775 pKa = 11.84 INAIWNLKK783 pKa = 9.62 VDD785 pKa = 4.48 LDD787 pKa = 4.23 SPISINRR794 pKa = 11.84 NISSISSSNAQAIQRR809 pKa = 11.84 VNAYY813 pKa = 10.05 LSEE816 pKa = 4.08 GVFDD820 pKa = 6.36 DD821 pKa = 3.81 FLKK824 pKa = 11.14 DD825 pKa = 2.98 KK826 pKa = 10.86 GRR828 pKa = 11.84 RR829 pKa = 3.55
Molecular weight: 94.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.091
IPC2_protein 7.527
IPC_protein 7.483
Toseland 7.614
ProMoST 8.126
Dawson 8.258
Bjellqvist 8.302
Wikipedia 8.244
Rodwell 8.317
Grimsley 7.688
Solomon 8.375
Lehninger 8.375
Nozaki 8.404
DTASelect 8.229
Thurlkill 8.317
EMBOSS 8.478
Sillero 8.521
Patrickios 4.66
IPC_peptide 8.375
IPC2_peptide 7.19
IPC2.peptide.svr19 7.499
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
829
829
829
829.0
94.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.911 ± 0.0
0.483 ± 0.0
5.79 ± 0.0
5.308 ± 0.0
4.101 ± 0.0
4.825 ± 0.0
1.809 ± 0.0
8.806 ± 0.0
6.273 ± 0.0
10.856 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.619 ± 0.0
5.79 ± 0.0
3.619 ± 0.0
3.981 ± 0.0
5.187 ± 0.0
7.358 ± 0.0
5.669 ± 0.0
6.514 ± 0.0
1.327 ± 0.0
2.774 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here