Amino acid dipepetide frequency for Brevibacillus phage Sundance

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.173AlaAla: 0.173 ± 0.066
0.47AlaCys: 0.47 ± 0.128
2.773AlaAsp: 2.773 ± 0.273
3.565AlaGlu: 3.565 ± 0.522
1.931AlaPhe: 1.931 ± 0.228
2.401AlaGly: 2.401 ± 0.295
0.495AlaHis: 0.495 ± 0.106
4.407AlaIle: 4.407 ± 0.33
5.001AlaLys: 5.001 ± 0.372
4.531AlaLeu: 4.531 ± 0.35
1.609AlaMet: 1.609 ± 0.229
3.268AlaAsn: 3.268 ± 0.276
1.213AlaPro: 1.213 ± 0.209
1.634AlaGln: 1.634 ± 0.198
1.956AlaArg: 1.956 ± 0.238
3.763AlaSer: 3.763 ± 0.329
3.392AlaThr: 3.392 ± 0.316
3.194AlaVal: 3.194 ± 0.315
0.644AlaTrp: 0.644 ± 0.166
2.773AlaTyr: 2.773 ± 0.24
0.0AlaXaa: 0.0 ± 0.0
Cys
0.47CysAla: 0.47 ± 0.112
0.124CysCys: 0.124 ± 0.049
0.446CysAsp: 0.446 ± 0.129
0.619CysGlu: 0.619 ± 0.155
0.322CysPhe: 0.322 ± 0.098
0.767CysGly: 0.767 ± 0.2
0.272CysHis: 0.272 ± 0.077
0.619CysIle: 0.619 ± 0.132
1.164CysLys: 1.164 ± 0.201
0.569CysLeu: 0.569 ± 0.13
0.223CysMet: 0.223 ± 0.086
0.545CysAsn: 0.545 ± 0.106
0.272CysPro: 0.272 ± 0.094
0.421CysGln: 0.421 ± 0.096
0.322CysArg: 0.322 ± 0.101
0.421CysSer: 0.421 ± 0.12
0.52CysThr: 0.52 ± 0.109
0.718CysVal: 0.718 ± 0.141
0.05CysTrp: 0.05 ± 0.036
0.47CysTyr: 0.47 ± 0.124
0.0CysXaa: 0.0 ± 0.0
Asp
3.119AspAla: 3.119 ± 0.344
0.569AspCys: 0.569 ± 0.137
4.011AspAsp: 4.011 ± 0.376
5.818AspGlu: 5.818 ± 0.472
4.085AspPhe: 4.085 ± 0.309
4.605AspGly: 4.605 ± 0.374
0.347AspHis: 0.347 ± 0.102
4.729AspIle: 4.729 ± 0.36
5.57AspLys: 5.57 ± 0.339
4.654AspLeu: 4.654 ± 0.365
2.129AspMet: 2.129 ± 0.229
3.59AspAsn: 3.59 ± 0.312
0.966AspPro: 0.966 ± 0.179
0.867AspGln: 0.867 ± 0.179
2.203AspArg: 2.203 ± 0.225
4.432AspSer: 4.432 ± 0.303
3.169AspThr: 3.169 ± 0.3
4.506AspVal: 4.506 ± 0.353
1.089AspTrp: 1.089 ± 0.162
3.194AspTyr: 3.194 ± 0.344
0.0AspXaa: 0.0 ± 0.0
Glu
4.605GluAla: 4.605 ± 0.374
0.842GluCys: 0.842 ± 0.203
4.927GluAsp: 4.927 ± 0.383
7.724GluGlu: 7.724 ± 0.573
2.748GluPhe: 2.748 ± 0.298
4.679GluGly: 4.679 ± 0.352
1.411GluHis: 1.411 ± 0.201
6.784GluIle: 6.784 ± 0.428
7.279GluLys: 7.279 ± 0.551
7.749GluLeu: 7.749 ± 0.7
2.674GluMet: 2.674 ± 0.29
5.051GluAsn: 5.051 ± 0.385
1.287GluPro: 1.287 ± 0.224
3.317GluGln: 3.317 ± 0.329
3.268GluArg: 3.268 ± 0.266
4.407GluSer: 4.407 ± 0.296
3.293GluThr: 3.293 ± 0.339
5.001GluVal: 5.001 ± 0.42
1.015GluTrp: 1.015 ± 0.181
3.417GluTyr: 3.417 ± 0.333
0.0GluXaa: 0.0 ± 0.0
Phe
2.253PheAla: 2.253 ± 0.219
0.396PheCys: 0.396 ± 0.104
3.268PheAsp: 3.268 ± 0.299
4.134PheGlu: 4.134 ± 0.38
1.634PhePhe: 1.634 ± 0.239
3.045PheGly: 3.045 ± 0.263
0.842PheHis: 0.842 ± 0.157
3.689PheIle: 3.689 ± 0.361
3.813PheLys: 3.813 ± 0.302
3.392PheLeu: 3.392 ± 0.365
1.164PheMet: 1.164 ± 0.168
2.6PheAsn: 2.6 ± 0.294
0.916PhePro: 0.916 ± 0.155
1.114PheGln: 1.114 ± 0.128
1.832PheArg: 1.832 ± 0.208
2.451PheSer: 2.451 ± 0.281
2.699PheThr: 2.699 ± 0.301
2.798PheVal: 2.798 ± 0.346
0.446PheTrp: 0.446 ± 0.114
2.228PheTyr: 2.228 ± 0.236
0.0PheXaa: 0.0 ± 0.0
Gly
2.352GlyAla: 2.352 ± 0.275
0.619GlyCys: 0.619 ± 0.142
3.887GlyAsp: 3.887 ± 0.42
4.481GlyGlu: 4.481 ± 0.312
2.748GlyPhe: 2.748 ± 0.273
3.441GlyGly: 3.441 ± 0.386
1.065GlyHis: 1.065 ± 0.186
4.456GlyIle: 4.456 ± 0.386
5.546GlyLys: 5.546 ± 0.408
5.125GlyLeu: 5.125 ± 0.358
1.56GlyMet: 1.56 ± 0.202
3.664GlyAsn: 3.664 ± 0.3
0.025GlyPro: 0.025 ± 0.021
2.03GlyGln: 2.03 ± 0.248
2.104GlyArg: 2.104 ± 0.209
3.738GlySer: 3.738 ± 0.395
3.417GlyThr: 3.417 ± 0.362
4.234GlyVal: 4.234 ± 0.347
1.015GlyTrp: 1.015 ± 0.147
3.565GlyTyr: 3.565 ± 0.358
0.0GlyXaa: 0.0 ± 0.0
His
0.966HisAla: 0.966 ± 0.134
0.124HisCys: 0.124 ± 0.055
1.04HisAsp: 1.04 ± 0.166
0.966HisGlu: 0.966 ± 0.136
0.966HisPhe: 0.966 ± 0.178
1.089HisGly: 1.089 ± 0.161
0.272HisHis: 0.272 ± 0.081
1.634HisIle: 1.634 ± 0.196
1.485HisLys: 1.485 ± 0.226
1.436HisLeu: 1.436 ± 0.204
0.47HisMet: 0.47 ± 0.1
0.891HisAsn: 0.891 ± 0.151
0.47HisPro: 0.47 ± 0.117
0.421HisGln: 0.421 ± 0.101
0.817HisArg: 0.817 ± 0.147
1.114HisSer: 1.114 ± 0.194
0.792HisThr: 0.792 ± 0.184
0.941HisVal: 0.941 ± 0.162
0.173HisTrp: 0.173 ± 0.06
0.792HisTyr: 0.792 ± 0.135
0.0HisXaa: 0.0 ± 0.0
Ile
4.531IleAla: 4.531 ± 0.287
0.767IleCys: 0.767 ± 0.143
5.694IleAsp: 5.694 ± 0.433
7.254IleGlu: 7.254 ± 0.47
2.674IlePhe: 2.674 ± 0.263
4.357IleGly: 4.357 ± 0.326
1.114IleHis: 1.114 ± 0.21
5.249IleIle: 5.249 ± 0.442
7.65IleLys: 7.65 ± 0.447
5.125IleLeu: 5.125 ± 0.4
1.56IleMet: 1.56 ± 0.246
5.447IleAsn: 5.447 ± 0.368
2.055IlePro: 2.055 ± 0.232
2.5IleGln: 2.5 ± 0.313
3.367IleArg: 3.367 ± 0.248
4.333IleSer: 4.333 ± 0.307
4.333IleThr: 4.333 ± 0.388
5.1IleVal: 5.1 ± 0.35
0.867IleTrp: 0.867 ± 0.155
2.822IleTyr: 2.822 ± 0.315
0.0IleXaa: 0.0 ± 0.0
Lys
5.075LysAla: 5.075 ± 0.398
0.693LysCys: 0.693 ± 0.173
5.818LysAsp: 5.818 ± 0.393
7.724LysGlu: 7.724 ± 0.457
3.417LysPhe: 3.417 ± 0.276
5.298LysGly: 5.298 ± 0.273
1.312LysHis: 1.312 ± 0.215
6.536LysIle: 6.536 ± 0.382
8.021LysLys: 8.021 ± 0.711
7.378LysLeu: 7.378 ± 0.458
3.169LysMet: 3.169 ± 0.262
5.546LysAsn: 5.546 ± 0.443
2.575LysPro: 2.575 ± 0.268
3.936LysGln: 3.936 ± 0.477
4.234LysArg: 4.234 ± 0.411
5.372LysSer: 5.372 ± 0.465
4.333LysThr: 4.333 ± 0.298
6.09LysVal: 6.09 ± 0.331
0.99LysTrp: 0.99 ± 0.182
4.234LysTyr: 4.234 ± 0.324
0.0LysXaa: 0.0 ± 0.0
Leu
4.234LeuAla: 4.234 ± 0.288
0.619LeuCys: 0.619 ± 0.143
4.902LeuAsp: 4.902 ± 0.35
6.437LeuGlu: 6.437 ± 0.499
3.367LeuPhe: 3.367 ± 0.326
4.06LeuGly: 4.06 ± 0.327
1.312LeuHis: 1.312 ± 0.161
5.397LeuIle: 5.397 ± 0.33
7.229LeuLys: 7.229 ± 0.414
5.471LeuLeu: 5.471 ± 0.387
2.179LeuMet: 2.179 ± 0.254
5.224LeuAsn: 5.224 ± 0.418
2.179LeuPro: 2.179 ± 0.268
3.045LeuGln: 3.045 ± 0.351
3.441LeuArg: 3.441 ± 0.352
5.57LeuSer: 5.57 ± 0.33
4.506LeuThr: 4.506 ± 0.351
5.471LeuVal: 5.471 ± 0.351
0.718LeuTrp: 0.718 ± 0.144
3.714LeuTyr: 3.714 ± 0.289
0.0LeuXaa: 0.0 ± 0.0
Met
1.832MetAla: 1.832 ± 0.222
0.248MetCys: 0.248 ± 0.079
1.485MetAsp: 1.485 ± 0.19
2.154MetGlu: 2.154 ± 0.223
1.535MetPhe: 1.535 ± 0.272
1.56MetGly: 1.56 ± 0.201
0.272MetHis: 0.272 ± 0.081
2.129MetIle: 2.129 ± 0.238
3.218MetLys: 3.218 ± 0.266
1.684MetLeu: 1.684 ± 0.193
0.842MetMet: 0.842 ± 0.138
1.708MetAsn: 1.708 ± 0.2
0.718MetPro: 0.718 ± 0.113
0.916MetGln: 0.916 ± 0.151
1.114MetArg: 1.114 ± 0.16
2.154MetSer: 2.154 ± 0.231
1.807MetThr: 1.807 ± 0.183
1.485MetVal: 1.485 ± 0.193
0.124MetTrp: 0.124 ± 0.053
0.99MetTyr: 0.99 ± 0.16
0.0MetXaa: 0.0 ± 0.0
Asn
3.07AsnAla: 3.07 ± 0.328
0.495AsnCys: 0.495 ± 0.114
3.936AsnAsp: 3.936 ± 0.403
4.927AsnGlu: 4.927 ± 0.353
2.575AsnPhe: 2.575 ± 0.218
4.531AsnGly: 4.531 ± 0.382
1.139AsnHis: 1.139 ± 0.168
4.951AsnIle: 4.951 ± 0.351
5.298AsnLys: 5.298 ± 0.392
4.951AsnLeu: 4.951 ± 0.391
1.188AsnMet: 1.188 ± 0.177
4.134AsnAsn: 4.134 ± 0.366
2.055AsnPro: 2.055 ± 0.198
2.426AsnGln: 2.426 ± 0.254
3.07AsnArg: 3.07 ± 0.269
3.169AsnSer: 3.169 ± 0.269
2.699AsnThr: 2.699 ± 0.286
3.936AsnVal: 3.936 ± 0.254
0.693AsnTrp: 0.693 ± 0.111
2.897AsnTyr: 2.897 ± 0.274
0.0AsnXaa: 0.0 ± 0.0
Pro
0.966ProAla: 0.966 ± 0.161
0.272ProCys: 0.272 ± 0.088
1.708ProAsp: 1.708 ± 0.205
1.882ProGlu: 1.882 ± 0.237
1.089ProPhe: 1.089 ± 0.147
0.074ProGly: 0.074 ± 0.041
0.594ProHis: 0.594 ± 0.106
1.931ProIle: 1.931 ± 0.277
2.352ProLys: 2.352 ± 0.206
1.758ProLeu: 1.758 ± 0.238
0.569ProMet: 0.569 ± 0.144
1.609ProAsn: 1.609 ± 0.203
0.198ProPro: 0.198 ± 0.08
0.668ProGln: 0.668 ± 0.137
0.99ProArg: 0.99 ± 0.171
1.609ProSer: 1.609 ± 0.187
1.733ProThr: 1.733 ± 0.253
1.882ProVal: 1.882 ± 0.236
0.173ProTrp: 0.173 ± 0.065
1.634ProTyr: 1.634 ± 0.23
0.0ProXaa: 0.0 ± 0.0
Gln
2.278GlnAla: 2.278 ± 0.255
0.248GlnCys: 0.248 ± 0.078
1.535GlnAsp: 1.535 ± 0.186
2.798GlnGlu: 2.798 ± 0.341
1.312GlnPhe: 1.312 ± 0.197
1.906GlnGly: 1.906 ± 0.227
0.668GlnHis: 0.668 ± 0.118
2.847GlnIle: 2.847 ± 0.331
2.971GlnLys: 2.971 ± 0.368
2.921GlnLeu: 2.921 ± 0.265
1.114GlnMet: 1.114 ± 0.195
1.882GlnAsn: 1.882 ± 0.263
0.668GlnPro: 0.668 ± 0.105
1.56GlnGln: 1.56 ± 0.302
1.56GlnArg: 1.56 ± 0.196
1.535GlnSer: 1.535 ± 0.182
1.733GlnThr: 1.733 ± 0.191
2.228GlnVal: 2.228 ± 0.218
0.421GlnTrp: 0.421 ± 0.099
1.708GlnTyr: 1.708 ± 0.211
0.0GlnXaa: 0.0 ± 0.0
Arg
1.733ArgAla: 1.733 ± 0.182
0.421ArgCys: 0.421 ± 0.113
2.476ArgAsp: 2.476 ± 0.303
3.293ArgGlu: 3.293 ± 0.367
2.426ArgPhe: 2.426 ± 0.229
2.203ArgGly: 2.203 ± 0.267
0.569ArgHis: 0.569 ± 0.122
3.54ArgIle: 3.54 ± 0.278
4.06ArgLys: 4.06 ± 0.335
3.763ArgLeu: 3.763 ± 0.268
1.362ArgMet: 1.362 ± 0.174
2.327ArgAsn: 2.327 ± 0.255
0.842ArgPro: 0.842 ± 0.159
1.535ArgGln: 1.535 ± 0.204
1.931ArgArg: 1.931 ± 0.195
1.931ArgSer: 1.931 ± 0.185
1.733ArgThr: 1.733 ± 0.194
2.649ArgVal: 2.649 ± 0.272
0.594ArgTrp: 0.594 ± 0.132
1.882ArgTyr: 1.882 ± 0.223
0.0ArgXaa: 0.0 ± 0.0
Ser
2.649SerAla: 2.649 ± 0.343
0.718SerCys: 0.718 ± 0.152
3.664SerAsp: 3.664 ± 0.262
3.565SerGlu: 3.565 ± 0.347
3.218SerPhe: 3.218 ± 0.313
4.283SerGly: 4.283 ± 0.355
1.337SerHis: 1.337 ± 0.192
5.298SerIle: 5.298 ± 0.349
5.694SerLys: 5.694 ± 0.393
4.555SerLeu: 4.555 ± 0.378
1.337SerMet: 1.337 ± 0.181
3.714SerAsn: 3.714 ± 0.315
1.634SerPro: 1.634 ± 0.188
1.882SerGln: 1.882 ± 0.25
1.807SerArg: 1.807 ± 0.223
4.209SerSer: 4.209 ± 0.31
3.466SerThr: 3.466 ± 0.304
3.54SerVal: 3.54 ± 0.337
0.594SerTrp: 0.594 ± 0.113
3.144SerTyr: 3.144 ± 0.28
0.0SerXaa: 0.0 ± 0.0
Thr
2.426ThrAla: 2.426 ± 0.351
0.347ThrCys: 0.347 ± 0.108
3.144ThrAsp: 3.144 ± 0.235
4.035ThrGlu: 4.035 ± 0.308
2.996ThrPhe: 2.996 ± 0.332
3.095ThrGly: 3.095 ± 0.286
1.015ThrHis: 1.015 ± 0.169
4.432ThrIle: 4.432 ± 0.319
4.976ThrLys: 4.976 ± 0.382
4.63ThrLeu: 4.63 ± 0.325
1.287ThrMet: 1.287 ± 0.202
2.748ThrAsn: 2.748 ± 0.25
2.03ThrPro: 2.03 ± 0.259
1.535ThrGln: 1.535 ± 0.21
1.906ThrArg: 1.906 ± 0.219
2.773ThrSer: 2.773 ± 0.278
2.921ThrThr: 2.921 ± 0.281
3.615ThrVal: 3.615 ± 0.271
0.644ThrTrp: 0.644 ± 0.138
2.699ThrTyr: 2.699 ± 0.271
0.0ThrXaa: 0.0 ± 0.0
Val
3.317ValAla: 3.317 ± 0.277
0.668ValCys: 0.668 ± 0.132
5.1ValAsp: 5.1 ± 0.387
5.595ValGlu: 5.595 ± 0.388
2.971ValPhe: 2.971 ± 0.34
3.936ValGly: 3.936 ± 0.309
1.485ValHis: 1.485 ± 0.21
4.184ValIle: 4.184 ± 0.434
5.422ValLys: 5.422 ± 0.346
5.026ValLeu: 5.026 ± 0.422
1.783ValMet: 1.783 ± 0.182
4.184ValAsn: 4.184 ± 0.295
2.055ValPro: 2.055 ± 0.268
2.03ValGln: 2.03 ± 0.248
2.674ValArg: 2.674 ± 0.292
3.788ValSer: 3.788 ± 0.345
3.169ValThr: 3.169 ± 0.302
5.026ValVal: 5.026 ± 0.429
0.767ValTrp: 0.767 ± 0.123
2.921ValTyr: 2.921 ± 0.289
0.0ValXaa: 0.0 ± 0.0
Trp
0.619TrpAla: 0.619 ± 0.138
0.124TrpCys: 0.124 ± 0.063
0.842TrpAsp: 0.842 ± 0.167
1.015TrpGlu: 1.015 ± 0.136
0.47TrpPhe: 0.47 ± 0.136
0.594TrpGly: 0.594 ± 0.125
0.347TrpHis: 0.347 ± 0.096
0.619TrpIle: 0.619 ± 0.109
0.916TrpLys: 0.916 ± 0.136
0.891TrpLeu: 0.891 ± 0.154
0.297TrpMet: 0.297 ± 0.08
0.916TrpAsn: 0.916 ± 0.173
0.0TrpPro: 0.0 ± 0.0
0.421TrpGln: 0.421 ± 0.108
0.495TrpArg: 0.495 ± 0.098
0.817TrpSer: 0.817 ± 0.133
0.767TrpThr: 0.767 ± 0.148
0.817TrpVal: 0.817 ± 0.139
0.099TrpTrp: 0.099 ± 0.052
0.47TrpTyr: 0.47 ± 0.104
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.55TyrAla: 2.55 ± 0.227
0.569TyrCys: 0.569 ± 0.137
2.996TyrAsp: 2.996 ± 0.265
3.491TyrGlu: 3.491 ± 0.27
2.228TyrPhe: 2.228 ± 0.283
3.144TyrGly: 3.144 ± 0.347
0.99TyrHis: 0.99 ± 0.18
3.367TyrIle: 3.367 ± 0.326
4.085TyrLys: 4.085 ± 0.319
3.466TyrLeu: 3.466 ± 0.354
1.436TyrMet: 1.436 ± 0.162
3.095TyrAsn: 3.095 ± 0.342
1.535TyrPro: 1.535 ± 0.189
1.634TyrGln: 1.634 ± 0.209
2.179TyrArg: 2.179 ± 0.213
2.723TyrSer: 2.723 ± 0.35
2.773TyrThr: 2.773 ± 0.31
2.872TyrVal: 2.872 ± 0.275
0.421TyrTrp: 0.421 ± 0.107
2.352TyrTyr: 2.352 ± 0.319
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 194 proteins (40393 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski