Pseudomonas phage KPP22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Pbunavirus; unclassified Pbunavirus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U5A906|A0A0U5A906_BPK22 Uncharacterized protein OS=Pseudomonas phage KPP22 OX=1772250 PE=4 SV=1
MM1 pKa = 7.53SGHH4 pKa = 6.25SLFDD8 pKa = 3.96FEE10 pKa = 5.22VDD12 pKa = 3.95CNSATNSDD20 pKa = 3.62TMKK23 pKa = 10.0IDD25 pKa = 3.57PGSFYY30 pKa = 11.31VGFGFGATALSVLIYY45 pKa = 10.73VDD47 pKa = 4.22GVWVSEE53 pKa = 4.11RR54 pKa = 11.84TAWIPDD60 pKa = 3.61PKK62 pKa = 10.43EE63 pKa = 3.61ISPP66 pKa = 4.12

Molecular weight: 7.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4VUF6|A0A0U4VUF6_BPK22 Uncharacterized protein OS=Pseudomonas phage KPP22 OX=1772250 PE=4 SV=1
MM1 pKa = 7.12YY2 pKa = 7.79RR3 pKa = 11.84TPSSKK8 pKa = 9.39FTFICNGLAQNAEE21 pKa = 4.2VTVLLRR27 pKa = 11.84KK28 pKa = 9.38QKK30 pKa = 10.78PEE32 pKa = 4.08DD33 pKa = 3.2VWVVDD38 pKa = 4.13KK39 pKa = 11.43APAANLIRR47 pKa = 11.84IHH49 pKa = 6.12EE50 pKa = 4.34RR51 pKa = 11.84SNPRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84TISILDD64 pKa = 3.44VDD66 pKa = 4.59AVSILHH72 pKa = 6.23KK73 pKa = 10.57RR74 pKa = 3.43

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

19485

53

1035

226.6

25.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.162 ± 0.31

0.939 ± 0.101

6.297 ± 0.215

7.036 ± 0.406

4.008 ± 0.187

7.303 ± 0.271

1.776 ± 0.166

6.025 ± 0.198

5.491 ± 0.378

7.375 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.458 ± 0.111

4.265 ± 0.35

4.578 ± 0.219

3.803 ± 0.153

6.076 ± 0.26

6.22 ± 0.205

5.158 ± 0.221

6.574 ± 0.252

1.545 ± 0.135

2.91 ± 0.101
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski