Enterobacter sp. EA-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacter; unclassified Enterobacter

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1052 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I7CA80|A0A2I7CA80_9ENTR Uncharacterized protein OS=Enterobacter sp. EA-1 OX=2057026 GN=CWS02_07705 PE=4 SV=1
MM1 pKa = 7.39SKK3 pKa = 10.5CSADD7 pKa = 3.62EE8 pKa = 4.37TPVCCCMDD16 pKa = 3.25VGTIMDD22 pKa = 4.5NSDD25 pKa = 3.17CTASYY30 pKa = 11.33SRR32 pKa = 11.84VFSNRR37 pKa = 11.84TDD39 pKa = 3.47AEE41 pKa = 4.05EE42 pKa = 3.81TLAALSARR50 pKa = 11.84ARR52 pKa = 11.84SVEE55 pKa = 4.01SDD57 pKa = 3.12PCQITSSFTEE67 pKa = 4.07VAGGVQLDD75 pKa = 3.27IDD77 pKa = 3.87FVFACEE83 pKa = 3.99AEE85 pKa = 4.22TLIFQPRR92 pKa = 11.84SALIFLPALPFFGSVLISFSVPP114 pKa = 2.89

Molecular weight: 12.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I7CAL6|A0A2I7CAL6_9ENTR Uncharacterized protein OS=Enterobacter sp. EA-1 OX=2057026 GN=CWS02_11910 PE=4 SV=1
MM1 pKa = 7.33VSPGLCHH8 pKa = 6.76GSLRR12 pKa = 11.84LRR14 pKa = 11.84ASSGHH19 pKa = 5.39VNAGSAGTGCGDD31 pKa = 3.98AYY33 pKa = 11.19LHH35 pKa = 6.49IASFPARR42 pKa = 11.84PMANDD47 pKa = 3.31SSGCDD52 pKa = 3.23GGGHH56 pKa = 5.76VSGNDD61 pKa = 2.84VWRR64 pKa = 11.84FAVPRR69 pKa = 11.84LHH71 pKa = 6.36QCGTVLVTAAVPVGFGMDD89 pKa = 3.64PFHH92 pKa = 7.75AGLLVLAVFAGNLSIKK108 pKa = 10.15PATTPLIRR116 pKa = 11.84WLGFKK121 pKa = 10.42KK122 pKa = 10.54LLLINGGLNVLGLLACALLTSHH144 pKa = 6.31TPVWLTFVILYY155 pKa = 9.95LGGVFRR161 pKa = 11.84SVQFTGVSTLAFSDD175 pKa = 4.21VPSSQMSYY183 pKa = 11.56ANTLFSTATQLAVGLGITLGAIGIRR208 pKa = 11.84IGEE211 pKa = 4.34YY212 pKa = 9.17VSQWLHH218 pKa = 4.29ITAVEE223 pKa = 4.7GISFRR228 pKa = 11.84LAFVFIALICPVGMVDD244 pKa = 4.49TLRR247 pKa = 11.84LTKK250 pKa = 10.49NAGSAVSQKK259 pKa = 9.86KK260 pKa = 7.11QRR262 pKa = 11.84KK263 pKa = 5.38TGRR266 pKa = 11.84NGALRR271 pKa = 11.84PADD274 pKa = 3.58YY275 pKa = 10.28QARR278 pKa = 11.84TSRR281 pKa = 11.84TNASISLLWQFSKK294 pKa = 10.23KK295 pKa = 9.84HH296 pKa = 4.79CWKK299 pKa = 10.29RR300 pKa = 11.84SPEE303 pKa = 4.14TAVFTSSRR311 pKa = 11.84SIARR315 pKa = 11.84RR316 pKa = 11.84VSRR319 pKa = 4.27

Molecular weight: 33.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1052

0

1052

132499

16

498

125.9

13.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.673 ± 0.094

1.654 ± 0.052

4.894 ± 0.078

5.069 ± 0.097

3.788 ± 0.072

7.164 ± 0.103

2.408 ± 0.051

5.505 ± 0.071

4.144 ± 0.083

10.208 ± 0.106

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.955 ± 0.043

3.796 ± 0.069

4.59 ± 0.074

4.156 ± 0.085

6.77 ± 0.095

6.521 ± 0.085

5.692 ± 0.074

6.765 ± 0.072

1.561 ± 0.047

2.688 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski