Fly associated circular virus 6
Average proteome isoelectric point is 7.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPC5|A0A346BPC5_9VIRU Putative capsid protein OS=Fly associated circular virus 6 OX=2293286 PE=4 SV=1
MM1 pKa = 7.82 AGGARR6 pKa = 11.84 NWCGTLQLEE15 pKa = 4.92 DD16 pKa = 5.18 DD17 pKa = 4.49 DD18 pKa = 4.64 WDD20 pKa = 4.52 AEE22 pKa = 4.34 TWLEE26 pKa = 4.24 SLVRR30 pKa = 11.84 DD31 pKa = 4.13 EE32 pKa = 3.98 KK33 pKa = 11.21 AKK35 pKa = 10.74 YY36 pKa = 9.99 VVGQIEE42 pKa = 4.26 RR43 pKa = 11.84 GHH45 pKa = 5.12 EE46 pKa = 3.97 TEE48 pKa = 4.29 HH49 pKa = 6.64 AHH51 pKa = 5.82 LQFYY55 pKa = 7.16 VQCLSQKK62 pKa = 10.26 NLVWMKK68 pKa = 11.01 RR69 pKa = 11.84 NINDD73 pKa = 3.47 KK74 pKa = 10.9 AHH76 pKa = 6.63 WEE78 pKa = 4.22 TAKK81 pKa = 9.75 GTAKK85 pKa = 10.44 HH86 pKa = 5.41 NRR88 pKa = 11.84 AYY90 pKa = 8.9 CTKK93 pKa = 9.0 EE94 pKa = 3.74 DD95 pKa = 3.63 SRR97 pKa = 11.84 VRR99 pKa = 11.84 GPWEE103 pKa = 3.8 FGEE106 pKa = 4.72 IVQQGQRR113 pKa = 11.84 EE114 pKa = 4.24 GLEE117 pKa = 4.16 SAIKK121 pKa = 9.09 MVKK124 pKa = 9.96 EE125 pKa = 3.73 GAPLKK130 pKa = 10.73 DD131 pKa = 2.96 IALEE135 pKa = 3.92 QSVVWVHH142 pKa = 5.28 HH143 pKa = 6.15 HH144 pKa = 6.79 RR145 pKa = 11.84 GLMDD149 pKa = 3.74 LRR151 pKa = 11.84 KK152 pKa = 9.54 QLGLEE157 pKa = 3.53 ADD159 pKa = 3.36 RR160 pKa = 11.84 RR161 pKa = 11.84 YY162 pKa = 10.55 FGVDD166 pKa = 3.76 GPEE169 pKa = 4.32 VWCLWGPSGTGKK181 pKa = 10.36 SRR183 pKa = 11.84 WVNAKK188 pKa = 9.52 WPDD191 pKa = 3.85 AFWKK195 pKa = 10.38 IPNEE199 pKa = 4.1 KK200 pKa = 8.78 WWDD203 pKa = 3.68 GYY205 pKa = 9.32 TGQEE209 pKa = 4.07 TVILDD214 pKa = 3.71 DD215 pKa = 5.26 FKK217 pKa = 11.64 DD218 pKa = 3.41 GDD220 pKa = 3.81 MRR222 pKa = 11.84 LGDD225 pKa = 4.32 LQRR228 pKa = 11.84 LLDD231 pKa = 4.47 WYY233 pKa = 9.17 PLWVEE238 pKa = 4.35 VKK240 pKa = 10.63 GGTIPMLAKK249 pKa = 10.18 RR250 pKa = 11.84 YY251 pKa = 9.76 VFTCNTHH258 pKa = 5.62 PSSWYY263 pKa = 10.04 RR264 pKa = 11.84 KK265 pKa = 8.75 ADD267 pKa = 3.33 PHH269 pKa = 5.02 RR270 pKa = 11.84 TIMRR274 pKa = 11.84 RR275 pKa = 11.84 FDD277 pKa = 3.06 EE278 pKa = 4.67 FAAQKK283 pKa = 10.58 GRR285 pKa = 11.84 LIMCEE290 pKa = 3.74 TDD292 pKa = 2.79 DD293 pKa = 4.3 WEE295 pKa = 4.88 INLEE299 pKa = 4.13 TRR301 pKa = 11.84 LGDD304 pKa = 3.76 LGNPQTPEE312 pKa = 3.68 VGGNTSTPTSGAPLRR327 pKa = 11.84 VPEE330 pKa = 4.11 NSEE333 pKa = 4.95 DD334 pKa = 3.51 IMIPSPMDD342 pKa = 3.71 FYY344 pKa = 12.01 VV345 pKa = 3.06
Molecular weight: 39.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.489
IPC2_protein 5.448
IPC_protein 5.448
Toseland 5.69
ProMoST 5.703
Dawson 5.588
Bjellqvist 5.652
Wikipedia 5.55
Rodwell 5.55
Grimsley 5.779
Solomon 5.588
Lehninger 5.575
Nozaki 5.804
DTASelect 5.982
Thurlkill 5.868
EMBOSS 5.83
Sillero 5.893
Patrickios 4.291
IPC_peptide 5.601
IPC2_peptide 5.893
IPC2.peptide.svr19 5.892
Protein with the highest isoelectric point:
>tr|A0A346BPC5|A0A346BPC5_9VIRU Putative capsid protein OS=Fly associated circular virus 6 OX=2293286 PE=4 SV=1
MM1 pKa = 7.09 AWSRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 TRR9 pKa = 11.84 FQRR12 pKa = 11.84 QRR14 pKa = 11.84 PRR16 pKa = 11.84 RR17 pKa = 11.84 LRR19 pKa = 11.84 RR20 pKa = 11.84 ARR22 pKa = 11.84 RR23 pKa = 11.84 LTRR26 pKa = 11.84 TRR28 pKa = 11.84 FRR30 pKa = 11.84 RR31 pKa = 11.84 YY32 pKa = 9.02 RR33 pKa = 11.84 PRR35 pKa = 11.84 GGGTRR40 pKa = 11.84 SATVKK45 pKa = 9.47 LTAEE49 pKa = 3.99 TTYY52 pKa = 11.35 APWQGPLTNVQAGWKK67 pKa = 8.59 PVAFTPQNLQGFNDD81 pKa = 3.83 YY82 pKa = 11.07 QSVYY86 pKa = 10.44 SQFRR90 pKa = 11.84 VKK92 pKa = 10.59 KK93 pKa = 9.84 CVVKK97 pKa = 10.68 INRR100 pKa = 11.84 GPDD103 pKa = 3.1 TNLVYY108 pKa = 10.78 LVVPSRR114 pKa = 11.84 AFAQTAAPSSSIANSWLAYY133 pKa = 9.96 VPPQTEE139 pKa = 3.47 QALRR143 pKa = 11.84 QTRR146 pKa = 11.84 WQKK149 pKa = 11.06 EE150 pKa = 4.06 LMPSTTAGKK159 pKa = 10.23 VRR161 pKa = 11.84 FGFHH165 pKa = 7.7 PYY167 pKa = 10.38 TMITAMGPIAQGQSINFTRR186 pKa = 11.84 VWNLNKK192 pKa = 7.35 WTPMSWALAQYY203 pKa = 9.28 PMYY206 pKa = 10.18 MYY208 pKa = 10.93 GPYY211 pKa = 10.51 LCLNNALTNADD222 pKa = 3.92 PDD224 pKa = 3.8 LTTSVSCIIEE234 pKa = 4.47 CYY236 pKa = 10.22 FQFKK240 pKa = 9.02 GQKK243 pKa = 9.15
Molecular weight: 28.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.266
IPC2_protein 10.248
IPC_protein 11.199
Toseland 11.125
ProMoST 11.345
Dawson 11.199
Bjellqvist 11.067
Wikipedia 11.55
Rodwell 11.184
Grimsley 11.257
Solomon 11.506
Lehninger 11.433
Nozaki 11.125
DTASelect 11.067
Thurlkill 11.14
EMBOSS 11.579
Sillero 11.155
Patrickios 10.877
IPC_peptide 11.506
IPC2_peptide 10.423
IPC2.peptide.svr19 8.348
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
588
243
345
294.0
33.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.973 ± 1.104
1.701 ± 0.036
5.102 ± 2.285
5.272 ± 2.397
3.401 ± 0.744
7.313 ± 1.298
1.871 ± 0.965
3.401 ± 0.344
5.272 ± 0.765
7.313 ± 0.482
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.891 ± 0.007
4.082 ± 0.566
5.952 ± 0.962
5.442 ± 1.299
8.333 ± 1.564
4.592 ± 0.773
7.143 ± 1.535
5.952 ± 0.126
4.422 ± 0.747
3.571 ± 0.904
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here