Amino acid dipepetide frequency for Escherichia virus Lambda_2H10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.095AlaAla: 16.095 ± 2.995
0.77AlaCys: 0.77 ± 0.248
6.315AlaAsp: 6.315 ± 0.808
5.853AlaGlu: 5.853 ± 0.908
3.312AlaPhe: 3.312 ± 0.363
9.241AlaGly: 9.241 ± 1.112
1.771AlaHis: 1.771 ± 0.411
4.698AlaIle: 4.698 ± 0.533
3.466AlaLys: 3.466 ± 0.747
7.855AlaLeu: 7.855 ± 0.979
3.389AlaMet: 3.389 ± 0.469
2.695AlaAsn: 2.695 ± 0.567
3.62AlaPro: 3.62 ± 0.618
3.697AlaGln: 3.697 ± 0.864
5.93AlaArg: 5.93 ± 0.993
8.779AlaSer: 8.779 ± 2.764
6.238AlaThr: 6.238 ± 1.573
6.854AlaVal: 6.854 ± 0.765
2.233AlaTrp: 2.233 ± 0.492
3.389AlaTyr: 3.389 ± 0.469
0.0AlaXaa: 0.0 ± 0.0
Cys
1.001CysAla: 1.001 ± 0.321
0.385CysCys: 0.385 ± 0.186
0.385CysAsp: 0.385 ± 0.179
0.77CysGlu: 0.77 ± 0.247
0.539CysPhe: 0.539 ± 0.193
0.847CysGly: 0.847 ± 0.252
0.308CysHis: 0.308 ± 0.165
0.462CysIle: 0.462 ± 0.172
0.308CysLys: 0.308 ± 0.143
0.616CysLeu: 0.616 ± 0.263
0.154CysMet: 0.154 ± 0.1
0.539CysAsn: 0.539 ± 0.168
0.308CysPro: 0.308 ± 0.144
0.693CysGln: 0.693 ± 0.214
1.155CysArg: 1.155 ± 0.26
0.77CysSer: 0.77 ± 0.263
0.616CysThr: 0.616 ± 0.247
0.539CysVal: 0.539 ± 0.181
0.616CysTrp: 0.616 ± 0.216
0.308CysTyr: 0.308 ± 0.134
0.0CysXaa: 0.0 ± 0.0
Asp
5.853AspAla: 5.853 ± 0.728
0.462AspCys: 0.462 ± 0.172
3.928AspAsp: 3.928 ± 0.559
3.697AspGlu: 3.697 ± 0.548
1.771AspPhe: 1.771 ± 0.366
5.853AspGly: 5.853 ± 0.691
0.308AspHis: 0.308 ± 0.136
3.543AspIle: 3.543 ± 0.498
2.926AspLys: 2.926 ± 0.421
4.544AspLeu: 4.544 ± 0.579
1.309AspMet: 1.309 ± 0.336
2.695AspAsn: 2.695 ± 0.456
2.387AspPro: 2.387 ± 0.629
1.463AspGln: 1.463 ± 0.326
2.464AspArg: 2.464 ± 0.464
3.466AspSer: 3.466 ± 0.428
3.08AspThr: 3.08 ± 0.511
3.466AspVal: 3.466 ± 0.437
1.155AspTrp: 1.155 ± 0.278
1.54AspTyr: 1.54 ± 0.309
0.0AspXaa: 0.0 ± 0.0
Glu
6.623GluAla: 6.623 ± 0.873
0.616GluCys: 0.616 ± 0.217
3.235GluAsp: 3.235 ± 0.444
4.467GluGlu: 4.467 ± 0.622
1.925GluPhe: 1.925 ± 0.408
3.62GluGly: 3.62 ± 0.661
1.078GluHis: 1.078 ± 0.368
3.235GluIle: 3.235 ± 0.446
4.159GluLys: 4.159 ± 0.651
5.545GluLeu: 5.545 ± 0.756
1.386GluMet: 1.386 ± 0.375
2.618GluAsn: 2.618 ± 0.445
3.235GluPro: 3.235 ± 0.553
4.621GluGln: 4.621 ± 0.673
3.851GluArg: 3.851 ± 0.709
3.851GluSer: 3.851 ± 0.541
4.082GluThr: 4.082 ± 0.539
3.928GluVal: 3.928 ± 0.567
1.078GluTrp: 1.078 ± 0.283
1.386GluTyr: 1.386 ± 0.306
0.0GluXaa: 0.0 ± 0.0
Phe
2.156PheAla: 2.156 ± 0.457
0.77PheCys: 0.77 ± 0.244
2.541PheAsp: 2.541 ± 0.442
2.464PheGlu: 2.464 ± 0.457
0.924PhePhe: 0.924 ± 0.226
1.848PheGly: 1.848 ± 0.337
0.693PheHis: 0.693 ± 0.251
1.617PheIle: 1.617 ± 0.31
1.386PheLys: 1.386 ± 0.41
1.848PheLeu: 1.848 ± 0.392
0.847PheMet: 0.847 ± 0.255
1.694PheAsn: 1.694 ± 0.278
1.617PhePro: 1.617 ± 0.343
0.693PheGln: 0.693 ± 0.201
2.695PheArg: 2.695 ± 0.541
2.772PheSer: 2.772 ± 0.474
2.464PheThr: 2.464 ± 0.468
2.618PheVal: 2.618 ± 0.367
0.616PheTrp: 0.616 ± 0.211
1.232PheTyr: 1.232 ± 0.338
0.0PheXaa: 0.0 ± 0.0
Gly
6.623GlyAla: 6.623 ± 0.975
0.77GlyCys: 0.77 ± 0.224
4.929GlyAsp: 4.929 ± 0.571
4.775GlyGlu: 4.775 ± 0.553
2.464GlyPhe: 2.464 ± 0.442
5.468GlyGly: 5.468 ± 0.794
0.847GlyHis: 0.847 ± 0.258
3.851GlyIle: 3.851 ± 0.483
5.16GlyLys: 5.16 ± 0.587
4.698GlyLeu: 4.698 ± 0.573
2.079GlyMet: 2.079 ± 0.372
3.543GlyAsn: 3.543 ± 0.463
1.463GlyPro: 1.463 ± 0.313
3.157GlyGln: 3.157 ± 0.572
4.082GlyArg: 4.082 ± 0.478
4.852GlySer: 4.852 ± 0.517
4.467GlyThr: 4.467 ± 0.636
5.391GlyVal: 5.391 ± 0.501
1.848GlyTrp: 1.848 ± 0.352
2.541GlyTyr: 2.541 ± 0.416
0.0GlyXaa: 0.0 ± 0.0
His
1.463HisAla: 1.463 ± 0.357
0.308HisCys: 0.308 ± 0.171
1.386HisAsp: 1.386 ± 0.356
0.539HisGlu: 0.539 ± 0.179
1.232HisPhe: 1.232 ± 0.361
0.924HisGly: 0.924 ± 0.246
0.231HisHis: 0.231 ± 0.164
1.155HisIle: 1.155 ± 0.301
1.001HisLys: 1.001 ± 0.255
1.54HisLeu: 1.54 ± 0.333
0.154HisMet: 0.154 ± 0.11
0.539HisAsn: 0.539 ± 0.211
0.462HisPro: 0.462 ± 0.196
0.539HisGln: 0.539 ± 0.232
1.001HisArg: 1.001 ± 0.267
1.386HisSer: 1.386 ± 0.472
1.309HisThr: 1.309 ± 0.447
1.386HisVal: 1.386 ± 0.231
0.385HisTrp: 0.385 ± 0.195
0.539HisTyr: 0.539 ± 0.147
0.0HisXaa: 0.0 ± 0.0
Ile
4.005IleAla: 4.005 ± 0.539
1.001IleCys: 1.001 ± 0.3
2.695IleAsp: 2.695 ± 0.468
4.313IleGlu: 4.313 ± 0.677
1.232IlePhe: 1.232 ± 0.364
3.235IleGly: 3.235 ± 0.452
0.693IleHis: 0.693 ± 0.227
2.002IleIle: 2.002 ± 0.412
3.08IleLys: 3.08 ± 0.499
2.772IleLeu: 2.772 ± 0.404
1.232IleMet: 1.232 ± 0.305
2.002IleAsn: 2.002 ± 0.314
2.695IlePro: 2.695 ± 0.424
1.232IleGln: 1.232 ± 0.323
3.543IleArg: 3.543 ± 0.557
4.621IleSer: 4.621 ± 0.623
3.774IleThr: 3.774 ± 0.812
2.541IleVal: 2.541 ± 0.366
0.462IleTrp: 0.462 ± 0.254
1.001IleTyr: 1.001 ± 0.274
0.0IleXaa: 0.0 ± 0.0
Lys
4.621LysAla: 4.621 ± 0.859
1.078LysCys: 1.078 ± 0.363
3.08LysAsp: 3.08 ± 0.488
3.62LysGlu: 3.62 ± 0.453
1.386LysPhe: 1.386 ± 0.286
4.467LysGly: 4.467 ± 0.673
1.078LysHis: 1.078 ± 0.272
2.849LysIle: 2.849 ± 0.496
3.62LysLys: 3.62 ± 0.557
4.005LysLeu: 4.005 ± 0.623
1.001LysMet: 1.001 ± 0.336
1.848LysAsn: 1.848 ± 0.414
1.463LysPro: 1.463 ± 0.234
1.54LysGln: 1.54 ± 0.302
4.082LysArg: 4.082 ± 0.469
3.928LysSer: 3.928 ± 0.616
4.082LysThr: 4.082 ± 0.569
3.851LysVal: 3.851 ± 0.453
1.155LysTrp: 1.155 ± 0.317
1.771LysTyr: 1.771 ± 0.397
0.0LysXaa: 0.0 ± 0.0
Leu
6.854LeuAla: 6.854 ± 0.988
0.924LeuCys: 0.924 ± 0.335
4.39LeuAsp: 4.39 ± 0.569
4.082LeuGlu: 4.082 ± 0.631
2.387LeuPhe: 2.387 ± 0.402
3.851LeuGly: 3.851 ± 0.54
1.694LeuHis: 1.694 ± 0.405
3.003LeuIle: 3.003 ± 0.483
4.005LeuLys: 4.005 ± 0.601
5.545LeuLeu: 5.545 ± 0.552
1.848LeuMet: 1.848 ± 0.329
3.466LeuAsn: 3.466 ± 0.476
4.005LeuPro: 4.005 ± 0.58
3.157LeuGln: 3.157 ± 0.457
6.084LeuArg: 6.084 ± 0.551
7.162LeuSer: 7.162 ± 0.722
6.084LeuThr: 6.084 ± 0.829
4.698LeuVal: 4.698 ± 0.531
1.232LeuTrp: 1.232 ± 0.274
1.309LeuTyr: 1.309 ± 0.334
0.0LeuXaa: 0.0 ± 0.0
Met
2.618MetAla: 2.618 ± 0.538
0.0MetCys: 0.0 ± 0.0
0.847MetAsp: 0.847 ± 0.214
0.847MetGlu: 0.847 ± 0.284
0.77MetPhe: 0.77 ± 0.23
1.232MetGly: 1.232 ± 0.27
0.385MetHis: 0.385 ± 0.177
0.77MetIle: 0.77 ± 0.247
1.463MetLys: 1.463 ± 0.312
2.31MetLeu: 2.31 ± 0.413
0.616MetMet: 0.616 ± 0.199
1.463MetAsn: 1.463 ± 0.291
1.54MetPro: 1.54 ± 0.359
1.155MetGln: 1.155 ± 0.29
1.617MetArg: 1.617 ± 0.305
1.694MetSer: 1.694 ± 0.352
2.926MetThr: 2.926 ± 0.608
1.925MetVal: 1.925 ± 0.387
0.231MetTrp: 0.231 ± 0.107
0.385MetTyr: 0.385 ± 0.221
0.0MetXaa: 0.0 ± 0.0
Asn
4.005AsnAla: 4.005 ± 0.775
0.847AsnCys: 0.847 ± 0.238
1.925AsnAsp: 1.925 ± 0.316
2.695AsnGlu: 2.695 ± 0.422
1.54AsnPhe: 1.54 ± 0.397
3.62AsnGly: 3.62 ± 0.554
0.462AsnHis: 0.462 ± 0.156
2.618AsnIle: 2.618 ± 0.471
2.233AsnLys: 2.233 ± 0.47
2.464AsnLeu: 2.464 ± 0.424
1.001AsnMet: 1.001 ± 0.28
2.233AsnAsn: 2.233 ± 0.404
2.002AsnPro: 2.002 ± 0.322
1.848AsnGln: 1.848 ± 0.358
2.31AsnArg: 2.31 ± 0.483
2.387AsnSer: 2.387 ± 0.571
2.233AsnThr: 2.233 ± 0.399
2.156AsnVal: 2.156 ± 0.333
0.539AsnTrp: 0.539 ± 0.177
1.078AsnTyr: 1.078 ± 0.252
0.0AsnXaa: 0.0 ± 0.0
Pro
5.083ProAla: 5.083 ± 0.661
0.308ProCys: 0.308 ± 0.179
3.62ProAsp: 3.62 ± 0.53
3.62ProGlu: 3.62 ± 0.766
0.924ProPhe: 0.924 ± 0.331
3.697ProGly: 3.697 ± 0.437
1.078ProHis: 1.078 ± 0.304
0.77ProIle: 0.77 ± 0.186
2.079ProLys: 2.079 ± 0.414
2.849ProLeu: 2.849 ± 0.642
0.616ProMet: 0.616 ± 0.186
1.155ProAsn: 1.155 ± 0.343
1.771ProPro: 1.771 ± 0.39
1.232ProGln: 1.232 ± 0.296
1.771ProArg: 1.771 ± 0.398
2.541ProSer: 2.541 ± 0.456
2.002ProThr: 2.002 ± 0.41
4.698ProVal: 4.698 ± 0.584
0.462ProTrp: 0.462 ± 0.192
0.847ProTyr: 0.847 ± 0.247
0.0ProXaa: 0.0 ± 0.0
Gln
4.159GlnAla: 4.159 ± 0.871
0.462GlnCys: 0.462 ± 0.172
1.078GlnAsp: 1.078 ± 0.23
3.003GlnGlu: 3.003 ± 0.444
1.309GlnPhe: 1.309 ± 0.304
2.156GlnGly: 2.156 ± 0.344
0.693GlnHis: 0.693 ± 0.219
2.541GlnIle: 2.541 ± 0.407
2.31GlnLys: 2.31 ± 0.355
3.389GlnLeu: 3.389 ± 0.472
1.232GlnMet: 1.232 ± 0.293
1.694GlnAsn: 1.694 ± 0.379
1.232GlnPro: 1.232 ± 0.348
1.771GlnGln: 1.771 ± 0.643
2.31GlnArg: 2.31 ± 0.401
3.003GlnSer: 3.003 ± 0.566
2.541GlnThr: 2.541 ± 0.449
2.387GlnVal: 2.387 ± 0.435
0.539GlnTrp: 0.539 ± 0.193
1.232GlnTyr: 1.232 ± 0.276
0.0GlnXaa: 0.0 ± 0.0
Arg
5.314ArgAla: 5.314 ± 0.631
0.77ArgCys: 0.77 ± 0.278
3.389ArgAsp: 3.389 ± 0.605
3.62ArgGlu: 3.62 ± 0.601
2.618ArgPhe: 2.618 ± 0.393
4.082ArgGly: 4.082 ± 0.54
1.463ArgHis: 1.463 ± 0.336
3.543ArgIle: 3.543 ± 0.636
3.466ArgLys: 3.466 ± 0.552
5.545ArgLeu: 5.545 ± 0.67
2.002ArgMet: 2.002 ± 0.376
2.541ArgAsn: 2.541 ± 0.481
2.541ArgPro: 2.541 ± 0.358
2.541ArgGln: 2.541 ± 0.544
5.545ArgArg: 5.545 ± 0.967
3.003ArgSer: 3.003 ± 0.437
3.08ArgThr: 3.08 ± 0.427
4.082ArgVal: 4.082 ± 0.757
1.078ArgTrp: 1.078 ± 0.364
2.541ArgTyr: 2.541 ± 0.322
0.0ArgXaa: 0.0 ± 0.0
Ser
11.398SerAla: 11.398 ± 3.281
0.385SerCys: 0.385 ± 0.182
4.159SerAsp: 4.159 ± 0.61
5.16SerGlu: 5.16 ± 0.707
2.464SerPhe: 2.464 ± 0.413
7.624SerGly: 7.624 ± 0.818
1.155SerHis: 1.155 ± 0.285
2.31SerIle: 2.31 ± 0.372
3.62SerLys: 3.62 ± 0.734
4.698SerLeu: 4.698 ± 0.636
1.848SerMet: 1.848 ± 0.385
1.848SerAsn: 1.848 ± 0.393
2.156SerPro: 2.156 ± 0.48
2.695SerGln: 2.695 ± 0.381
4.236SerArg: 4.236 ± 0.578
4.159SerSer: 4.159 ± 1.19
4.467SerThr: 4.467 ± 0.712
6.084SerVal: 6.084 ± 0.801
0.847SerTrp: 0.847 ± 0.199
1.54SerTyr: 1.54 ± 0.395
0.0SerXaa: 0.0 ± 0.0
Thr
7.624ThrAla: 7.624 ± 1.022
0.539ThrCys: 0.539 ± 0.201
3.157ThrAsp: 3.157 ± 0.552
4.159ThrGlu: 4.159 ± 0.586
2.618ThrPhe: 2.618 ± 0.464
5.006ThrGly: 5.006 ± 0.717
1.617ThrHis: 1.617 ± 0.385
2.695ThrIle: 2.695 ± 0.413
3.312ThrLys: 3.312 ± 0.558
5.468ThrLeu: 5.468 ± 0.648
1.078ThrMet: 1.078 ± 0.318
2.31ThrAsn: 2.31 ± 0.572
3.389ThrPro: 3.389 ± 0.676
2.156ThrGln: 2.156 ± 0.456
3.466ThrArg: 3.466 ± 0.486
5.006ThrSer: 5.006 ± 0.945
3.928ThrThr: 3.928 ± 0.594
4.236ThrVal: 4.236 ± 0.87
1.001ThrTrp: 1.001 ± 0.297
2.079ThrTyr: 2.079 ± 0.3
0.0ThrXaa: 0.0 ± 0.0
Val
5.853ValAla: 5.853 ± 0.643
0.231ValCys: 0.231 ± 0.134
2.31ValAsp: 2.31 ± 0.533
4.39ValGlu: 4.39 ± 0.58
2.464ValPhe: 2.464 ± 0.324
3.312ValGly: 3.312 ± 0.472
0.847ValHis: 0.847 ± 0.259
4.39ValIle: 4.39 ± 0.588
4.775ValLys: 4.775 ± 0.709
5.93ValLeu: 5.93 ± 0.733
2.31ValMet: 2.31 ± 0.351
3.774ValAsn: 3.774 ± 0.485
3.157ValPro: 3.157 ± 0.532
2.772ValGln: 2.772 ± 0.601
3.851ValArg: 3.851 ± 0.531
5.853ValSer: 5.853 ± 0.738
4.775ValThr: 4.775 ± 0.725
4.236ValVal: 4.236 ± 0.538
1.155ValTrp: 1.155 ± 0.386
1.848ValTyr: 1.848 ± 0.384
0.0ValXaa: 0.0 ± 0.0
Trp
1.694TrpAla: 1.694 ± 0.35
0.231TrpCys: 0.231 ± 0.124
0.847TrpAsp: 0.847 ± 0.326
0.77TrpGlu: 0.77 ± 0.231
0.693TrpPhe: 0.693 ± 0.253
0.847TrpGly: 0.847 ± 0.249
0.539TrpHis: 0.539 ± 0.235
0.847TrpIle: 0.847 ± 0.297
0.77TrpLys: 0.77 ± 0.231
1.925TrpLeu: 1.925 ± 0.398
0.539TrpMet: 0.539 ± 0.181
0.693TrpAsn: 0.693 ± 0.196
0.693TrpPro: 0.693 ± 0.245
0.77TrpGln: 0.77 ± 0.236
1.001TrpArg: 1.001 ± 0.299
0.77TrpSer: 0.77 ± 0.193
1.232TrpThr: 1.232 ± 0.28
1.232TrpVal: 1.232 ± 0.375
0.462TrpTrp: 0.462 ± 0.199
1.001TrpTyr: 1.001 ± 0.238
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.157TyrAla: 3.157 ± 0.439
0.462TyrCys: 0.462 ± 0.179
1.694TyrAsp: 1.694 ± 0.304
1.694TyrGlu: 1.694 ± 0.329
0.924TyrPhe: 0.924 ± 0.258
2.156TyrGly: 2.156 ± 0.39
0.539TyrHis: 0.539 ± 0.2
1.232TyrIle: 1.232 ± 0.33
1.386TyrLys: 1.386 ± 0.295
2.233TyrLeu: 2.233 ± 0.427
0.077TyrMet: 0.077 ± 0.074
0.847TyrAsn: 0.847 ± 0.185
1.386TyrPro: 1.386 ± 0.338
1.309TyrGln: 1.309 ± 0.221
1.771TyrArg: 1.771 ± 0.307
2.849TyrSer: 2.849 ± 0.502
1.463TyrThr: 1.463 ± 0.252
1.925TyrVal: 1.925 ± 0.426
0.462TyrTrp: 0.462 ± 0.173
0.616TyrTyr: 0.616 ± 0.179
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (12986 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski