Melissococcus plutonius (strain ATCC 35311 / CIP 104052 / LMG 20360 / NCIMB 702443)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Melissococcus; Melissococcus plutonius

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1876 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F3YA25|F3YA25_MELPT 6-phosphogluconolactonase OS=Melissococcus plutonius (strain ATCC 35311 / CIP 104052 / LMG 20360 / NCIMB 702443) OX=940190 GN=MPTP_0891 PE=3 SV=1
MM1 pKa = 7.16TLAKK5 pKa = 9.51IVYY8 pKa = 10.46ASMTGNTEE16 pKa = 3.92EE17 pKa = 4.26IADD20 pKa = 3.5IVTEE24 pKa = 4.18TFEE27 pKa = 4.41NLDD30 pKa = 3.17IDD32 pKa = 4.53VEE34 pKa = 4.5MNEE37 pKa = 4.28CTQAEE42 pKa = 4.32AEE44 pKa = 4.66DD45 pKa = 4.82FLDD48 pKa = 4.95ADD50 pKa = 3.74ICVVATYY57 pKa = 9.24TYY59 pKa = 11.39GDD61 pKa = 3.83GEE63 pKa = 4.56LPDD66 pKa = 4.96EE67 pKa = 4.49IVDD70 pKa = 4.47FYY72 pKa = 11.77EE73 pKa = 5.28DD74 pKa = 5.02LSEE77 pKa = 5.49LDD79 pKa = 4.4LNGKK83 pKa = 9.49IYY85 pKa = 9.98GVCGSGDD92 pKa = 3.21TFYY95 pKa = 11.45DD96 pKa = 3.95EE97 pKa = 4.63FCKK100 pKa = 10.74SVNDD104 pKa = 3.86FDD106 pKa = 5.62AVFAKK111 pKa = 9.85TGAMKK116 pKa = 10.41GAEE119 pKa = 4.06NVKK122 pKa = 10.18VDD124 pKa = 4.54LAPEE128 pKa = 4.22EE129 pKa = 4.51EE130 pKa = 4.4DD131 pKa = 3.64VQNLEE136 pKa = 4.11VFAKK140 pKa = 10.61SLVKK144 pKa = 10.43SLKK147 pKa = 10.31

Molecular weight: 16.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F3YCM0|F3YCM0_MELPT Uncharacterized protein OS=Melissococcus plutonius (strain ATCC 35311 / CIP 104052 / LMG 20360 / NCIMB 702443) OX=940190 GN=MPTP_1854 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 9.14RR10 pKa = 11.84KK11 pKa = 8.54HH12 pKa = 5.23QKK14 pKa = 8.76VHH16 pKa = 5.58GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 10.16NGRR28 pKa = 11.84HH29 pKa = 4.31VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.15GRR39 pKa = 11.84KK40 pKa = 8.82ALAAA44 pKa = 4.25

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1876

0

1876

527073

37

1450

281.0

31.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.241 ± 0.05

0.714 ± 0.016

4.858 ± 0.048

6.793 ± 0.064

4.647 ± 0.052

6.15 ± 0.053

1.962 ± 0.022

8.993 ± 0.068

7.837 ± 0.056

9.931 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.639 ± 0.024

5.478 ± 0.045

3.3 ± 0.028

4.443 ± 0.047

3.492 ± 0.039

5.809 ± 0.039

6.032 ± 0.038

5.911 ± 0.042

0.904 ± 0.019

3.864 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski