Wenzhou tombus-like virus 6

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH21|A0A1L3KH21_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 6 OX=1923676 PE=4 SV=1
MM1 pKa = 7.03KK2 pKa = 9.39TDD4 pKa = 3.55RR5 pKa = 11.84YY6 pKa = 10.49NFVVSYY12 pKa = 10.99GGYY15 pKa = 7.04TYY17 pKa = 11.42KK18 pKa = 10.65RR19 pKa = 11.84MEE21 pKa = 4.68FGTISVTLDD30 pKa = 3.08EE31 pKa = 5.04NEE33 pKa = 4.19VKK35 pKa = 10.64GVSRR39 pKa = 11.84PRR41 pKa = 11.84KK42 pKa = 6.04EE43 pKa = 4.22HH44 pKa = 6.16KK45 pKa = 10.31SISAAHH51 pKa = 5.99TLYY54 pKa = 10.24PDD56 pKa = 3.57YY57 pKa = 11.15EE58 pKa = 4.46SLLNASQEE66 pKa = 4.08QTLSEE71 pKa = 3.96EE72 pKa = 3.9SDD74 pKa = 3.48AIKK77 pKa = 10.35AYY79 pKa = 9.44EE80 pKa = 3.85QAANRR85 pKa = 11.84QGDD88 pKa = 3.87DD89 pKa = 3.72SEE91 pKa = 5.7SDD93 pKa = 3.89AEE95 pKa = 4.32VLPPPGYY102 pKa = 10.24EE103 pKa = 3.79PEE105 pKa = 4.59TIVGLPPPGPGRR117 pKa = 11.84VPYY120 pKa = 10.16RR121 pKa = 11.84ADD123 pKa = 4.48PISIPNVVVKK133 pKa = 9.69PSNNRR138 pKa = 11.84NTGSFLSRR146 pKa = 11.84LVEE149 pKa = 4.25SQKK152 pKa = 9.34TAPRR156 pKa = 11.84DD157 pKa = 3.6EE158 pKa = 4.45EE159 pKa = 4.44TLSVTSARR167 pKa = 11.84TTGSLTPEE175 pKa = 3.52EE176 pKa = 4.83RR177 pKa = 11.84IEE179 pKa = 3.64YY180 pKa = 8.0WRR182 pKa = 11.84IYY184 pKa = 10.01RR185 pKa = 11.84SRR187 pKa = 11.84GKK189 pKa = 10.23AAADD193 pKa = 4.38FYY195 pKa = 11.41KK196 pKa = 10.59KK197 pKa = 10.35RR198 pKa = 11.84IEE200 pKa = 4.56DD201 pKa = 3.53NPGG204 pKa = 2.74

Molecular weight: 22.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KH21|A0A1L3KH21_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 6 OX=1923676 PE=4 SV=1
MM1 pKa = 7.74IKK3 pKa = 10.27PIAPRR8 pKa = 11.84LICPRR13 pKa = 11.84TKK15 pKa = 10.14RR16 pKa = 11.84YY17 pKa = 9.81NVILGTRR24 pKa = 11.84LKK26 pKa = 10.63FNEE29 pKa = 3.93KK30 pKa = 10.03RR31 pKa = 11.84IMRR34 pKa = 11.84AIDD37 pKa = 3.54SVFGSPTVLSSYY49 pKa = 11.4DD50 pKa = 3.13SFAQGRR56 pKa = 11.84IIARR60 pKa = 11.84KK61 pKa = 7.61WAMFNNPVAIGVDD74 pKa = 3.46ASRR77 pKa = 11.84FDD79 pKa = 3.39QHH81 pKa = 8.72VSVQALQWEE90 pKa = 4.31HH91 pKa = 6.95SIYY94 pKa = 10.81NAIFHH99 pKa = 6.91DD100 pKa = 4.18PEE102 pKa = 4.16LRR104 pKa = 11.84EE105 pKa = 4.25ALSHH109 pKa = 5.22QLKK112 pKa = 10.8NNISLFVEE120 pKa = 4.35DD121 pKa = 3.82KK122 pKa = 10.25MIRR125 pKa = 11.84FKK127 pKa = 11.44VDD129 pKa = 2.51GHH131 pKa = 6.48RR132 pKa = 11.84MSGDD136 pKa = 2.98INTSMGNKK144 pKa = 10.23LIMCGLMHH152 pKa = 7.29HH153 pKa = 6.02YY154 pKa = 10.19FKK156 pKa = 10.71TLGVRR161 pKa = 11.84AEE163 pKa = 4.19LCNNGDD169 pKa = 3.8DD170 pKa = 4.92CVIICEE176 pKa = 4.47NKK178 pKa = 10.21DD179 pKa = 2.86KK180 pKa = 11.28HH181 pKa = 7.26LFDD184 pKa = 5.43NIYY187 pKa = 10.7EE188 pKa = 4.33HH189 pKa = 7.07FLQFGFNMVTEE200 pKa = 4.3PVVEE204 pKa = 4.15CLEE207 pKa = 4.11KK208 pKa = 11.32LEE210 pKa = 4.64FCQSRR215 pKa = 11.84PICVNGAWRR224 pKa = 11.84MVRR227 pKa = 11.84RR228 pKa = 11.84PDD230 pKa = 4.29SIAKK234 pKa = 9.99DD235 pKa = 3.52SHH237 pKa = 6.31TLLSMLNAEE246 pKa = 4.5DD247 pKa = 3.79VKK249 pKa = 11.57SFMSATAQCGLVLNSGVPILEE270 pKa = 3.83AFYY273 pKa = 10.33RR274 pKa = 11.84CLYY277 pKa = 10.12RR278 pKa = 11.84SSGYY282 pKa = 10.69KK283 pKa = 9.91KK284 pKa = 9.59VTEE287 pKa = 4.4EE288 pKa = 4.06YY289 pKa = 8.37IKK291 pKa = 11.05AVIGYY296 pKa = 9.48GNEE299 pKa = 3.64EE300 pKa = 4.03KK301 pKa = 10.93LQGRR305 pKa = 11.84RR306 pKa = 11.84NPVSIPVTDD315 pKa = 4.01STRR318 pKa = 11.84MSYY321 pKa = 9.33WEE323 pKa = 4.34SFGVDD328 pKa = 3.65PKK330 pKa = 9.66TQQIIEE336 pKa = 4.42RR337 pKa = 11.84YY338 pKa = 8.92YY339 pKa = 11.45DD340 pKa = 3.97DD341 pKa = 4.54LTVSTQLQPVKK352 pKa = 10.08LTTPHH357 pKa = 6.1LQSILLEE364 pKa = 4.49IPQHH368 pKa = 4.73PTII371 pKa = 4.71

Molecular weight: 42.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

954

204

379

318.0

36.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.128 ± 1.393

1.363 ± 0.717

5.031 ± 0.152

8.386 ± 1.471

3.354 ± 0.668

4.612 ± 0.771

2.83 ± 0.555

5.975 ± 0.981

6.184 ± 0.49

8.281 ± 0.946

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.935 ± 0.587

4.088 ± 0.669

5.136 ± 0.968

3.774 ± 0.398

6.394 ± 0.445

7.338 ± 0.809

5.346 ± 0.508

6.394 ± 0.361

1.258 ± 0.319

4.193 ± 0.53
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski