Microbacterium phage Didgeridoo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dismasvirus; unclassified Dismasvirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R4A142|A0A2R4A142_9CAUD Uncharacterized protein OS=Microbacterium phage Didgeridoo OX=2126928 GN=63 PE=4 SV=1
MM1 pKa = 7.48NNNEE5 pKa = 4.09TSILPHH11 pKa = 5.9VATMPVISKK20 pKa = 10.65VNLLAEE26 pKa = 5.42AILEE30 pKa = 4.32NKK32 pKa = 7.99GTVYY36 pKa = 9.67STANILVDD44 pKa = 6.02LIAIVCDD51 pKa = 3.32NAGFEE56 pKa = 4.49VEE58 pKa = 4.22LHH60 pKa = 5.58ITNEE64 pKa = 4.58GYY66 pKa = 10.77SLAFPQDD73 pKa = 3.33VEE75 pKa = 4.15ADD77 pKa = 3.82EE78 pKa = 4.61VLGALDD84 pKa = 3.18QGLEE88 pKa = 4.05VLEE91 pKa = 4.34EE92 pKa = 4.05MALEE96 pKa = 4.38DD97 pKa = 3.95

Molecular weight: 10.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4A165|A0A2R4A165_9CAUD Uncharacterized protein OS=Microbacterium phage Didgeridoo OX=2126928 GN=72 PE=4 SV=1
MM1 pKa = 7.47RR2 pKa = 11.84TTLIEE7 pKa = 4.21AGIGSLIGWLAGGVIAYY24 pKa = 9.16RR25 pKa = 11.84VYY27 pKa = 10.62RR28 pKa = 11.84RR29 pKa = 11.84AAQAYY34 pKa = 7.66QRR36 pKa = 11.84QFGFKK41 pKa = 9.69PP42 pKa = 3.26

Molecular weight: 4.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

13548

29

920

183.1

20.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.47 ± 0.323

0.472 ± 0.091

6.466 ± 0.275

6.038 ± 0.286

3.27 ± 0.146

8.068 ± 0.351

1.912 ± 0.171

4.621 ± 0.164

4.296 ± 0.267

8.599 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.155 ± 0.149

3.432 ± 0.214

5.492 ± 0.201

3.277 ± 0.172

6.156 ± 0.362

5.233 ± 0.347

7.056 ± 0.424

7.484 ± 0.289

2.089 ± 0.137

2.414 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski