Cronobacter phage vB_CsaM_GAP32

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Mimasvirus; Cronobacter virus GAP32

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 545 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4F7E3|K4F7E3_9CAUD Macro domain-containing protein OS=Cronobacter phage vB_CsaM_GAP32 OX=1141136 GN=GAP32_192 PE=4 SV=1
MM1 pKa = 6.91YY2 pKa = 10.62KK3 pKa = 10.5LYY5 pKa = 10.35RR6 pKa = 11.84FEE8 pKa = 3.89MDD10 pKa = 3.04YY11 pKa = 11.45GRR13 pKa = 11.84NGSLDD18 pKa = 3.53GLFISTDD25 pKa = 3.27EE26 pKa = 4.17EE27 pKa = 4.44LNLINNATIYY37 pKa = 10.55FGEE40 pKa = 4.25AFGKK44 pKa = 10.07HH45 pKa = 4.37SEE47 pKa = 3.87VWIDD51 pKa = 3.32DD52 pKa = 4.15FEE54 pKa = 4.45WEE56 pKa = 4.37KK57 pKa = 11.18CCTVISDD64 pKa = 3.84EE65 pKa = 4.16QEE67 pKa = 4.11KK68 pKa = 10.69IEE70 pKa = 4.04WLIDD74 pKa = 3.38LMGYY78 pKa = 7.37TLTGYY83 pKa = 10.66NPLDD87 pKa = 3.41YY88 pKa = 10.94FEE90 pKa = 4.49YY91 pKa = 10.13TEE93 pKa = 4.02SSEE96 pKa = 4.08YY97 pKa = 10.21QEE99 pKa = 6.12GYY101 pKa = 10.63DD102 pKa = 5.37ADD104 pKa = 4.91LEE106 pKa = 5.17DD107 pKa = 4.22MDD109 pKa = 5.68CEE111 pKa = 4.32YY112 pKa = 11.25EE113 pKa = 4.28MPGEE117 pKa = 3.72IARR120 pKa = 11.84WFKK123 pKa = 10.83GKK125 pKa = 9.17EE126 pKa = 3.67DD127 pKa = 3.23RR128 pKa = 11.84RR129 pKa = 11.84VYY131 pKa = 10.54EE132 pKa = 4.7FEE134 pKa = 5.66CKK136 pKa = 9.95DD137 pKa = 3.44TDD139 pKa = 5.41DD140 pKa = 5.27DD141 pKa = 5.06QDD143 pKa = 3.74DD144 pKa = 3.61

Molecular weight: 17.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4FB04|K4FB04_9CAUD Uncharacterized protein OS=Cronobacter phage vB_CsaM_GAP32 OX=1141136 GN=GAP32_051 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.51KK3 pKa = 9.0LTIDD7 pKa = 3.35NTQLRR12 pKa = 11.84VHH14 pKa = 6.77FKK16 pKa = 11.04ASTKK20 pKa = 10.37ILDD23 pKa = 3.41RR24 pKa = 11.84ALRR27 pKa = 11.84VVKK30 pKa = 10.26DD31 pKa = 4.08FNSGQIRR38 pKa = 11.84PRR40 pKa = 11.84RR41 pKa = 11.84LGCGLFEE48 pKa = 4.28VLDD51 pKa = 3.62VSRR54 pKa = 11.84NEE56 pKa = 4.18RR57 pKa = 11.84IVIKK61 pKa = 10.28DD62 pKa = 3.3RR63 pKa = 11.84KK64 pKa = 9.1LNLMTHH70 pKa = 5.34EE71 pKa = 4.33QYY73 pKa = 11.66NKK75 pKa = 9.79FVEE78 pKa = 4.44RR79 pKa = 11.84RR80 pKa = 3.48

Molecular weight: 9.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

545

0

545

107935

36

3341

198.0

22.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.571 ± 0.124

1.23 ± 0.066

6.591 ± 0.111

7.292 ± 0.197

4.515 ± 0.082

5.687 ± 0.129

1.955 ± 0.073

7.013 ± 0.087

6.83 ± 0.163

7.752 ± 0.094

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.075

6.275 ± 0.145

3.182 ± 0.076

3.591 ± 0.086

3.924 ± 0.088

6.833 ± 0.126

6.317 ± 0.263

6.738 ± 0.113

1.141 ± 0.043

4.789 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski