Pseudoalteromonas phenolica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4164 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2JZI6|A0A0S2JZI6_9GAMM Uncharacterized protein OS=Pseudoalteromonas phenolica OX=161398 GN=PP2015_781 PE=4 SV=1
MM1 pKa = 7.31NKK3 pKa = 10.05KK4 pKa = 10.27LLFSAALLAATSSSIFASSQTFQASINGWSEE35 pKa = 3.84PTFSEE40 pKa = 4.79ANALHH45 pKa = 6.37FGKK48 pKa = 10.35VRR50 pKa = 11.84LAAGSTCTMDD60 pKa = 3.09NAGAVTGDD68 pKa = 4.36CDD70 pKa = 4.98ASDD73 pKa = 5.02ANIQLGGITVSGLAPSSAMNITVSGSTGSNLTFAPATTATDD114 pKa = 3.86GTSTTTTADD123 pKa = 3.32GVASAFTTDD132 pKa = 4.67ASATDD137 pKa = 3.29ITINVYY143 pKa = 10.46GQVTVDD149 pKa = 3.24NALTAEE155 pKa = 4.24GNYY158 pKa = 9.11TVDD161 pKa = 3.34YY162 pKa = 9.34TVDD165 pKa = 3.13VSFQQ169 pKa = 3.17

Molecular weight: 16.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2K5U2|A0A0S2K5U2_9GAMM 5-carboxymethyl-2-hydroxymuconate delta-isomerase OS=Pseudoalteromonas phenolica OX=161398 GN=PP2015_2813 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.93VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4164

0

4164

1416653

30

6705

340.2

37.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.414 ± 0.039

0.99 ± 0.015

5.507 ± 0.038

6.441 ± 0.039

4.481 ± 0.028

6.277 ± 0.036

2.271 ± 0.018

6.353 ± 0.029

5.99 ± 0.036

10.544 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.345 ± 0.017

4.767 ± 0.026

3.596 ± 0.025

4.891 ± 0.035

3.895 ± 0.025

6.908 ± 0.037

5.35 ± 0.026

6.644 ± 0.029

1.133 ± 0.013

3.203 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski