Beihai tombus-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGT8|A0A1L3KGT8_9VIRU RNA-directed RNA polymerase OS=Beihai tombus-like virus 4 OX=1922725 PE=4 SV=1
MM1 pKa = 7.54AVGLGWRR8 pKa = 11.84VIACNRR14 pKa = 11.84LRR16 pKa = 11.84YY17 pKa = 9.65LCLLNFCLTRR27 pKa = 11.84NTNNKK32 pKa = 9.61RR33 pKa = 11.84NTNRR37 pKa = 11.84SQRR40 pKa = 11.84VAAPVATGLRR50 pKa = 11.84TSGGRR55 pKa = 11.84PQTTPAGAGAIRR67 pKa = 11.84VRR69 pKa = 11.84HH70 pKa = 5.85RR71 pKa = 11.84EE72 pKa = 3.84FIKK75 pKa = 10.98NITSEE80 pKa = 4.14HH81 pKa = 5.28TFEE84 pKa = 5.38NGVLEE89 pKa = 4.29LGINAGDD96 pKa = 3.45TEE98 pKa = 4.34MFPWLSNIANGYY110 pKa = 8.18EE111 pKa = 4.15RR112 pKa = 11.84YY113 pKa = 9.4CVNSMSISYY122 pKa = 10.14EE123 pKa = 3.86PFVSTTEE130 pKa = 3.83SGAVIMQVDD139 pKa = 4.58YY140 pKa = 11.38DD141 pKa = 4.22PADD144 pKa = 3.8EE145 pKa = 4.55PPISKK150 pKa = 9.74SHH152 pKa = 5.77MLNSLGATRR161 pKa = 11.84SAVWMRR167 pKa = 11.84SAMALNRR174 pKa = 11.84KK175 pKa = 7.73EE176 pKa = 4.86LSYY179 pKa = 11.33DD180 pKa = 2.93DD181 pKa = 4.41HH182 pKa = 8.57LFVRR186 pKa = 11.84HH187 pKa = 5.7AVRR190 pKa = 11.84AGFSEE195 pKa = 3.79NLKK198 pKa = 10.56LYY200 pKa = 10.67DD201 pKa = 3.43VGTVFIALTDD211 pKa = 3.77VPAPTTLSYY220 pKa = 11.58GEE222 pKa = 4.06VWVTYY227 pKa = 10.08DD228 pKa = 2.94ITLMVPAFHH237 pKa = 6.62KK238 pKa = 10.59QEE240 pKa = 4.23PNAAEE245 pKa = 4.22SKK247 pKa = 10.45VNQSNYY253 pKa = 9.85FDD255 pKa = 3.49ILGTISSDD263 pKa = 3.51RR264 pKa = 11.84PNTMLQGSSINFTTDD279 pKa = 2.37SNNAGEE285 pKa = 4.27CAITFQEE292 pKa = 4.68PFTGLLYY299 pKa = 10.86LEE301 pKa = 4.14QSGYY305 pKa = 11.31AEE307 pKa = 4.82DD308 pKa = 5.04SITHH312 pKa = 6.75LEE314 pKa = 4.32LEE316 pKa = 4.62PTTTPADD323 pKa = 3.13NWITKK328 pKa = 9.03LARR331 pKa = 11.84LGGGVIDD338 pKa = 6.63YY339 pKa = 10.91ILLDD343 pKa = 4.05NKK345 pKa = 9.24WKK347 pKa = 10.59YY348 pKa = 10.56LIEE351 pKa = 4.22VVADD355 pKa = 3.7AGEE358 pKa = 4.56SIVFDD363 pKa = 4.19ALAVGAGDD371 pKa = 3.57LTTWIGEE378 pKa = 4.16TAMALSPYY386 pKa = 10.46AEE388 pKa = 4.22ALMLPLIGLRR398 pKa = 11.84ASDD401 pKa = 3.29QNEE404 pKa = 3.14IRR406 pKa = 11.84RR407 pKa = 11.84RR408 pKa = 11.84IQSRR412 pKa = 11.84GKK414 pKa = 10.16HH415 pKa = 4.78NLIPAGDD422 pKa = 3.8VVDD425 pKa = 4.27RR426 pKa = 11.84STWVQSLVSGTQVEE440 pKa = 4.74EE441 pKa = 4.17EE442 pKa = 4.16

Molecular weight: 48.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGZ6|A0A1L3KGZ6_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 4 OX=1922725 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 5.23KK3 pKa = 10.74GEE5 pKa = 4.41RR6 pKa = 11.84IRR8 pKa = 11.84IRR10 pKa = 11.84KK11 pKa = 8.06ARR13 pKa = 11.84QVGRR17 pKa = 11.84SKK19 pKa = 10.79KK20 pKa = 9.32ARR22 pKa = 11.84TYY24 pKa = 10.52YY25 pKa = 10.15RR26 pKa = 11.84VNGGNGPDD34 pKa = 3.15WDD36 pKa = 4.22IPNNDD41 pKa = 3.49IEE43 pKa = 4.89TVTHH47 pKa = 6.71AVLEE51 pKa = 4.32RR52 pKa = 11.84VFFVKK57 pKa = 10.34DD58 pKa = 3.43GKK60 pKa = 11.21GGFQRR65 pKa = 11.84APKK68 pKa = 8.48PWSHH72 pKa = 6.97SSINAQEE79 pKa = 4.22NPVAQARR86 pKa = 11.84SYY88 pKa = 11.0VQDD91 pKa = 3.59KK92 pKa = 10.64LSQFSRR98 pKa = 11.84EE99 pKa = 3.87MEE101 pKa = 4.19RR102 pKa = 11.84CSKK105 pKa = 10.01IHH107 pKa = 6.26GVVSPCTNQEE117 pKa = 3.76FVEE120 pKa = 5.06CYY122 pKa = 10.25GGAKK126 pKa = 9.75RR127 pKa = 11.84KK128 pKa = 9.64IYY130 pKa = 9.12EE131 pKa = 3.94QAVEE135 pKa = 4.18SLEE138 pKa = 4.36SNPLQARR145 pKa = 11.84DD146 pKa = 3.42KK147 pKa = 10.21RR148 pKa = 11.84VKK150 pKa = 10.62VFTKK154 pKa = 10.62DD155 pKa = 3.11EE156 pKa = 4.04YY157 pKa = 11.13LKK159 pKa = 10.76PDD161 pKa = 3.92GAPRR165 pKa = 11.84AIQPRR170 pKa = 11.84SPRR173 pKa = 11.84FNVCLGRR180 pKa = 11.84YY181 pKa = 7.97VKK183 pKa = 10.37QLEE186 pKa = 4.17HH187 pKa = 7.31LIFEE191 pKa = 5.59AINEE195 pKa = 4.08IFDD198 pKa = 3.59GSGEE202 pKa = 4.28HH203 pKa = 5.5KK204 pKa = 9.63TVAKK208 pKa = 9.35GMNMNEE214 pKa = 3.89RR215 pKa = 11.84GEE217 pKa = 4.49EE218 pKa = 3.7IRR220 pKa = 11.84GMWEE224 pKa = 3.8KK225 pKa = 10.75YY226 pKa = 8.76VNPVAVGLDD235 pKa = 3.14ASRR238 pKa = 11.84FDD240 pKa = 3.43QHH242 pKa = 7.83INTLLLEE249 pKa = 4.72HH250 pKa = 6.09EE251 pKa = 4.35HH252 pKa = 6.66KK253 pKa = 10.26IYY255 pKa = 10.94RR256 pKa = 11.84MWSTGQGDD264 pKa = 4.77DD265 pKa = 4.8LPNLNTLLSAQLKK278 pKa = 9.67NKK280 pKa = 9.23GVYY283 pKa = 9.97VGIDD287 pKa = 3.82GILKK291 pKa = 10.31YY292 pKa = 10.29KK293 pKa = 11.01VNGCRR298 pKa = 11.84MSGDD302 pKa = 3.63MNTSLGNVIIMCSLMYY318 pKa = 10.51SYY320 pKa = 11.06FKK322 pKa = 10.57DD323 pKa = 3.35KK324 pKa = 11.57NMLGKK329 pKa = 10.14ISLLNDD335 pKa = 3.39GDD337 pKa = 4.0DD338 pKa = 3.94CVIIMEE344 pKa = 4.2QKK346 pKa = 9.92RR347 pKa = 11.84LKK349 pKa = 10.7EE350 pKa = 3.97FTRR353 pKa = 11.84GLKK356 pKa = 10.11DD357 pKa = 2.67WFLRR361 pKa = 11.84MGITMEE367 pKa = 3.88FDD369 pKa = 3.21GVYY372 pKa = 8.94NTLEE376 pKa = 3.96EE377 pKa = 4.85VEE379 pKa = 4.66FCQARR384 pKa = 11.84PVFNEE389 pKa = 3.31EE390 pKa = 3.58LGYY393 pKa = 11.21VLTPRR398 pKa = 11.84PSKK401 pKa = 10.46RR402 pKa = 11.84LYY404 pKa = 10.86SDD406 pKa = 4.16VISTKK411 pKa = 7.74MMKK414 pKa = 10.0SKK416 pKa = 10.5KK417 pKa = 9.85VYY419 pKa = 9.88RR420 pKa = 11.84KK421 pKa = 9.51QLGAIAGCGLAMSGGVPIFQQFYY444 pKa = 7.54TWMGRR449 pKa = 11.84GATPWVPKK457 pKa = 10.37MGDD460 pKa = 2.68HH461 pKa = 6.43YY462 pKa = 11.54YY463 pKa = 11.06KK464 pKa = 10.54FRR466 pKa = 11.84QEE468 pKa = 5.26LIDD471 pKa = 3.81GMTYY475 pKa = 10.07KK476 pKa = 10.32QRR478 pKa = 11.84EE479 pKa = 3.82PTMRR483 pKa = 11.84EE484 pKa = 3.49RR485 pKa = 11.84ISFYY489 pKa = 9.55FAHH492 pKa = 7.46GITPAEE498 pKa = 3.95QKK500 pKa = 10.81LIEE503 pKa = 4.82NYY505 pKa = 8.48YY506 pKa = 10.36QCLPDD511 pKa = 4.64PLWSKK516 pKa = 10.34PVIDD520 pKa = 4.02PEE522 pKa = 4.29RR523 pKa = 11.84YY524 pKa = 8.52IDD526 pKa = 3.64SVQYY530 pKa = 10.67LVEE533 pKa = 4.34PEE535 pKa = 4.06QKK537 pKa = 10.08FKK539 pKa = 11.07RR540 pKa = 11.84LSS542 pKa = 3.27

Molecular weight: 62.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1355

371

542

451.7

50.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.528 ± 1.602

1.402 ± 0.221

4.945 ± 0.032

7.306 ± 0.447

3.173 ± 0.523

8.044 ± 0.378

1.845 ± 0.015

5.387 ± 0.356

5.166 ± 1.386

7.749 ± 0.542

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.247 ± 0.33

5.166 ± 0.348

4.502 ± 0.146

3.321 ± 0.66

7.159 ± 0.588

6.347 ± 0.451

5.461 ± 0.986

6.863 ± 0.151

1.624 ± 0.079

3.764 ± 0.542
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski