Wenling narna-like virus 7
Average proteome isoelectric point is 9.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIV3|A0A1L3KIV3_9VIRU RNA-dependent RNA polymerase OS=Wenling narna-like virus 7 OX=1923507 PE=4 SV=1
MM1 pKa = 6.2 VTHH4 pKa = 7.15 HH5 pKa = 6.66 ARR7 pKa = 11.84 EE8 pKa = 4.0 EE9 pKa = 3.88 LAQGVGHH16 pKa = 6.91 PPRR19 pKa = 11.84 CEE21 pKa = 4.1 SEE23 pKa = 5.14 LSLQRR28 pKa = 11.84 PFLGPEE34 pKa = 3.46 EE35 pKa = 4.67 DD36 pKa = 3.6 AQFTLKK42 pKa = 10.35 FHH44 pKa = 7.3 RR45 pKa = 11.84 EE46 pKa = 4.04 VLDD49 pKa = 4.06 SFQTSQKK56 pKa = 10.19 VKK58 pKa = 10.1 PGWRR62 pKa = 11.84 CAPLADD68 pKa = 4.06 RR69 pKa = 11.84 EE70 pKa = 4.44 GRR72 pKa = 11.84 RR73 pKa = 11.84 GNPDD77 pKa = 2.64 VRR79 pKa = 11.84 VFSGHH84 pKa = 5.89 SLPGYY89 pKa = 9.83 RR90 pKa = 11.84 PADD93 pKa = 3.84 PFPAARR99 pKa = 11.84 GPLEE103 pKa = 4.05 VLPLAVQKK111 pKa = 9.41 PCCEE115 pKa = 4.03 AVSQPLGQRR124 pKa = 11.84 HH125 pKa = 5.27 AAHH128 pKa = 7.14 RR129 pKa = 11.84 SSQRR133 pKa = 11.84 RR134 pKa = 11.84 QAISPDD140 pKa = 3.39 YY141 pKa = 9.72 QRR143 pKa = 11.84 QGRR146 pKa = 11.84 RR147 pKa = 11.84 EE148 pKa = 3.89 SSLPRR153 pKa = 11.84 PAPNARR159 pKa = 11.84 VEE161 pKa = 4.11 DD162 pKa = 3.74 AKK164 pKa = 11.17 YY165 pKa = 10.79 SPNSHH170 pKa = 6.69 RR171 pKa = 11.84 GAKK174 pKa = 9.14 IHH176 pKa = 6.9 GSLHH180 pKa = 6.4 RR181 pKa = 11.84 CRR183 pKa = 11.84 QRR185 pKa = 11.84 SPGRR189 pKa = 11.84 SKK191 pKa = 10.84 AAILGRR197 pKa = 11.84 VKK199 pKa = 10.75 RR200 pKa = 11.84 SDD202 pKa = 3.73 QPPKK206 pKa = 10.73 GYY208 pKa = 10.41 AADD211 pKa = 4.04 GGEE214 pKa = 4.3 LCLNPIPLAHH224 pKa = 6.47 GRR226 pKa = 11.84 LPRR229 pKa = 11.84 RR230 pKa = 11.84 PGNRR234 pKa = 11.84 EE235 pKa = 3.84 EE236 pKa = 4.06 VCEE239 pKa = 3.97 RR240 pKa = 11.84 CSPRR244 pKa = 11.84 FRR246 pKa = 11.84 RR247 pKa = 11.84 IHH249 pKa = 4.94 EE250 pKa = 4.46 TVFLLLPRR258 pKa = 11.84 DD259 pKa = 3.64 LKK261 pKa = 11.39 DD262 pKa = 4.12 LFGEE266 pKa = 4.3 KK267 pKa = 10.21 NVVSGSVMFTDD278 pKa = 4.35 RR279 pKa = 11.84 EE280 pKa = 4.06 LRR282 pKa = 11.84 SAGFRR287 pKa = 11.84 HH288 pKa = 5.79 FLVSVGHH295 pKa = 6.08 FWWPDD300 pKa = 2.82 RR301 pKa = 11.84 RR302 pKa = 11.84 KK303 pKa = 10.29 VVATDD308 pKa = 3.33 DD309 pKa = 3.73 RR310 pKa = 11.84 PGRR313 pKa = 11.84 RR314 pKa = 11.84 CRR316 pKa = 11.84 LGGQGNVLEE325 pKa = 4.94 GEE327 pKa = 4.38 TPRR330 pKa = 11.84 RR331 pKa = 11.84 GPGRR335 pKa = 11.84 LGPKK339 pKa = 8.88 EE340 pKa = 3.95 VNEE343 pKa = 4.18 IKK345 pKa = 10.36 QGPRR349 pKa = 11.84 RR350 pKa = 11.84 RR351 pKa = 11.84 EE352 pKa = 3.8 AHH354 pKa = 6.87 HH355 pKa = 7.23 DD356 pKa = 3.69 PPPDD360 pKa = 3.78 RR361 pKa = 11.84 EE362 pKa = 4.31 TVAIRR367 pKa = 11.84 QTSIQGTCQFSEE379 pKa = 4.68 DD380 pKa = 4.21 LLNLCRR386 pKa = 11.84 WRR388 pKa = 11.84 LSRR391 pKa = 11.84 TGEE394 pKa = 3.8 EE395 pKa = 4.38 TIDD398 pKa = 3.4 KK399 pKa = 10.99 GRR401 pKa = 11.84 DD402 pKa = 3.2 QVLRR406 pKa = 11.84 EE407 pKa = 4.07 QICSGSQVGGEE418 pKa = 3.92 ADD420 pKa = 3.56 VLLLRR425 pKa = 11.84 RR426 pKa = 11.84 LLTVNPLGLSPEE438 pKa = 4.34 GPRR441 pKa = 11.84 VIPRR445 pKa = 11.84 QDD447 pKa = 3.23 ALHH450 pKa = 6.65 APVFPEE456 pKa = 3.86 TTEE459 pKa = 3.76
Molecular weight: 51.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.265
IPC2_protein 9.048
IPC_protein 9.268
Toseland 10.16
ProMoST 9.78
Dawson 10.277
Bjellqvist 9.999
Wikipedia 10.438
Rodwell 10.452
Grimsley 10.306
Solomon 10.35
Lehninger 10.335
Nozaki 10.262
DTASelect 9.94
Thurlkill 10.175
EMBOSS 10.555
Sillero 10.233
Patrickios 10.116
IPC_peptide 10.365
IPC2_peptide 9.253
IPC2.peptide.svr19 7.959
Protein with the highest isoelectric point:
>tr|A0A1L3KIV3|A0A1L3KIV3_9VIRU RNA-dependent RNA polymerase OS=Wenling narna-like virus 7 OX=1923507 PE=4 SV=1
MM1 pKa = 7.57 PRR3 pKa = 11.84 NKK5 pKa = 9.75 ARR7 pKa = 11.84 HH8 pKa = 5.33 RR9 pKa = 11.84 WVCLYY14 pKa = 10.71 EE15 pKa = 4.16 LTLSVATLLPARR27 pKa = 11.84 PGTGPYY33 pKa = 10.37 LGGTRR38 pKa = 11.84 PNGPDD43 pKa = 2.73 TGRR46 pKa = 11.84 FLPRR50 pKa = 11.84 FPRR53 pKa = 11.84 HH54 pKa = 5.44 PNIRR58 pKa = 11.84 CPKK61 pKa = 8.44 EE62 pKa = 3.67 RR63 pKa = 11.84 GNAATGKK70 pKa = 5.35 TTNGNVNGILSKK82 pKa = 9.92 VLRR85 pKa = 11.84 RR86 pKa = 11.84 RR87 pKa = 11.84 PLGNQRR93 pKa = 11.84 WTRR96 pKa = 11.84 LEE98 pKa = 4.04 EE99 pKa = 3.55 AWRR102 pKa = 11.84 TILAAIAACGFVRR115 pKa = 11.84 LDD117 pKa = 3.3 SSRR120 pKa = 11.84 RR121 pKa = 11.84 AHH123 pKa = 6.93 RR124 pKa = 11.84 EE125 pKa = 3.37 ALRR128 pKa = 11.84 GLSRR132 pKa = 11.84 LSTWIVKK139 pKa = 8.15 TAALSGTEE147 pKa = 3.85 EE148 pKa = 4.08 VLRR151 pKa = 11.84 EE152 pKa = 4.06 LKK154 pKa = 9.04 TWSARR159 pKa = 11.84 LRR161 pKa = 11.84 AHH163 pKa = 6.22 VVNSLPPHH171 pKa = 5.77 KK172 pKa = 9.81 RR173 pKa = 11.84 RR174 pKa = 11.84 RR175 pKa = 11.84 GFARR179 pKa = 11.84 YY180 pKa = 7.98 FQGVLRR186 pKa = 11.84 SLIDD190 pKa = 3.36 KK191 pKa = 10.42 DD192 pKa = 3.95 SRR194 pKa = 11.84 ALQMSYY200 pKa = 10.01 IGRR203 pKa = 11.84 ALPVGSFRR211 pKa = 11.84 EE212 pKa = 4.12 TEE214 pKa = 3.75 GAIRR218 pKa = 11.84 KK219 pKa = 8.55 FRR221 pKa = 11.84 EE222 pKa = 4.01 VTQVPFEE229 pKa = 4.1 THH231 pKa = 7.16 PDD233 pKa = 3.48 LLDD236 pKa = 4.47 SIRR239 pKa = 11.84 DD240 pKa = 3.73 WGAHH244 pKa = 3.75 WAARR248 pKa = 11.84 FLDD251 pKa = 4.48 TPAMPPLWRR260 pKa = 11.84 SSSGCVDD267 pKa = 4.23 FSRR270 pKa = 11.84 LQGGLAEE277 pKa = 4.33 AAVRR281 pKa = 11.84 AADD284 pKa = 4.26 FAAAEE289 pKa = 4.3 VEE291 pKa = 4.97 SRR293 pKa = 11.84 EE294 pKa = 4.29 DD295 pKa = 3.2 WDD297 pKa = 4.53 DD298 pKa = 3.74 LRR300 pKa = 11.84 NTAAEE305 pKa = 4.28 VPEE308 pKa = 4.18 MTDD311 pKa = 3.06 CDD313 pKa = 3.1 VDD315 pKa = 3.49 IAVRR319 pKa = 11.84 EE320 pKa = 4.05 AQLRR324 pKa = 11.84 DD325 pKa = 3.34 VALTVAAHH333 pKa = 6.66 LPDD336 pKa = 4.66 LPPVEE341 pKa = 4.5 VCTVPEE347 pKa = 4.05 RR348 pKa = 11.84 GMKK351 pKa = 9.98 CRR353 pKa = 11.84 IVTKK357 pKa = 10.61 APWCLIHH364 pKa = 7.1 IGHH367 pKa = 7.48 FLRR370 pKa = 11.84 SWLFGGLRR378 pKa = 11.84 KK379 pKa = 9.76 DD380 pKa = 3.34 RR381 pKa = 11.84 RR382 pKa = 11.84 VQGVLAGDD390 pKa = 3.36 HH391 pKa = 6.68 AGALRR396 pKa = 11.84 GQTEE400 pKa = 4.01 RR401 pKa = 11.84 VYY403 pKa = 10.44 SQKK406 pKa = 9.2 PTEE409 pKa = 4.32 EE410 pKa = 4.03 EE411 pKa = 4.23 NICLSADD418 pKa = 3.38 LTAATDD424 pKa = 4.46 LFPQDD429 pKa = 3.66 LVAALVDD436 pKa = 4.01 GLLSGARR443 pKa = 11.84 KK444 pKa = 8.99 PPTAEE449 pKa = 3.48 IQEE452 pKa = 4.4 VFRR455 pKa = 11.84 KK456 pKa = 8.53 LTGPLNARR464 pKa = 11.84 LPDD467 pKa = 3.84 GDD469 pKa = 3.64 GFAIRR474 pKa = 11.84 RR475 pKa = 11.84 GIMMGFPTTWTLLNLVNLFWSEE497 pKa = 4.37 TAWSASGSLPFQDD510 pKa = 3.8 VALSAQPAASPRR522 pKa = 11.84 TIVCGDD528 pKa = 3.87 DD529 pKa = 4.06 LASVWPPKK537 pKa = 10.3 VADD540 pKa = 4.4 RR541 pKa = 11.84 YY542 pKa = 10.33 EE543 pKa = 4.27 EE544 pKa = 3.99 VAEE547 pKa = 4.06 ACGAKK552 pKa = 10.26 FSVGKK557 pKa = 9.87 HH558 pKa = 4.02 YY559 pKa = 10.69 RR560 pKa = 11.84 SRR562 pKa = 11.84 DD563 pKa = 3.49 YY564 pKa = 11.22 ILFTEE569 pKa = 5.21 EE570 pKa = 3.45 IFKK573 pKa = 10.97 VSWEE577 pKa = 4.02 KK578 pKa = 10.89 QEE580 pKa = 3.91 YY581 pKa = 10.93 SLMDD585 pKa = 3.35 SAKK588 pKa = 10.03 PRR590 pKa = 11.84 RR591 pKa = 11.84 TTLADD596 pKa = 4.08 FFPVPRR602 pKa = 11.84 APRR605 pKa = 11.84 KK606 pKa = 7.65 STMRR610 pKa = 11.84 KK611 pKa = 9.22 RR612 pKa = 11.84 YY613 pKa = 9.13 RR614 pKa = 11.84 VQAKK618 pKa = 8.7 LAPVRR623 pKa = 11.84 CVPLRR628 pKa = 11.84 GLVRR632 pKa = 11.84 PLHH635 pKa = 5.74 APKK638 pKa = 10.29 DD639 pKa = 3.68 RR640 pKa = 11.84 RR641 pKa = 11.84 LRR643 pKa = 11.84 PSWAALPAAVEE654 pKa = 4.01 AAMDD658 pKa = 4.28 LGAPVAVRR666 pKa = 11.84 RR667 pKa = 11.84 VLRR670 pKa = 11.84 VLHH673 pKa = 5.96 PGIWCWARR681 pKa = 11.84 KK682 pKa = 9.5 RR683 pKa = 11.84 GFSPTLPLVVGGYY696 pKa = 9.83 GLPPLRR702 pKa = 11.84 GSVRR706 pKa = 11.84 RR707 pKa = 11.84 VSLPKK712 pKa = 8.97 WLRR715 pKa = 11.84 YY716 pKa = 9.37 GLAAWLLNSKK726 pKa = 9.32 WKK728 pKa = 10.43 DD729 pKa = 3.2 LEE731 pKa = 4.56 GPSRR735 pKa = 11.84 CWEE738 pKa = 4.41 GVCRR742 pKa = 11.84 PVSWQTMARR751 pKa = 11.84 EE752 pKa = 4.04 HH753 pKa = 6.7 ADD755 pKa = 3.18 VRR757 pKa = 11.84 IASSTFAVRR766 pKa = 11.84 KK767 pKa = 9.88 GRR769 pKa = 11.84 TPPPGFHH776 pKa = 7.12 LLGGLEE782 pKa = 4.64 AIQNLSVKK790 pKa = 10.38 FEE792 pKa = 4.36 GEE794 pKa = 4.01 LCVLLGTEE802 pKa = 4.16 EE803 pKa = 4.68 GSLKK807 pKa = 10.47 RR808 pKa = 11.84 QFGLAPRR815 pKa = 11.84 RR816 pKa = 11.84 VANSLRR822 pKa = 11.84 KK823 pKa = 9.23 FFARR827 pKa = 11.84 MVSDD831 pKa = 3.54 HH832 pKa = 7.0 PSRR835 pKa = 11.84 RR836 pKa = 11.84 PFPSSLGRR844 pKa = 11.84 TALVQRR850 pKa = 11.84 WRR852 pKa = 11.84 SRR854 pKa = 11.84 AEE856 pKa = 3.48 EE857 pKa = 3.85 RR858 pKa = 11.84 ALYY861 pKa = 7.48 STPRR865 pKa = 11.84 SAVEE869 pKa = 3.89 GLVLSLPVRR878 pKa = 11.84 AKK880 pKa = 10.59 RR881 pKa = 11.84 LLAEE885 pKa = 4.09 ALGWVV890 pKa = 3.71
Molecular weight: 99.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.443
IPC_protein 10.101
Toseland 10.584
ProMoST 10.262
Dawson 10.672
Bjellqvist 10.379
Wikipedia 10.877
Rodwell 10.818
Grimsley 10.716
Solomon 10.789
Lehninger 10.745
Nozaki 10.584
DTASelect 10.365
Thurlkill 10.57
EMBOSS 10.979
Sillero 10.599
Patrickios 10.496
IPC_peptide 10.789
IPC2_peptide 9.502
IPC2.peptide.svr19 8.633
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1349
459
890
674.5
75.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.192 ± 1.318
2.076 ± 0.247
4.448 ± 0.158
6.004 ± 0.543
3.484 ± 0.099
7.784 ± 0.527
2.52 ± 0.543
2.52 ± 0.057
3.632 ± 0.167
10.526 ± 0.631
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.038 ± 0.276
2.001 ± 0.081
8.08 ± 0.69
3.113 ± 0.87
12.305 ± 0.551
6.301 ± 0.008
4.448 ± 0.541
7.042 ± 0.332
2.224 ± 0.62
1.26 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here