Wenling narna-like virus 7

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 9.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KIV3|A0A1L3KIV3_9VIRU RNA-dependent RNA polymerase OS=Wenling narna-like virus 7 OX=1923507 PE=4 SV=1
MM1 pKa = 6.2VTHH4 pKa = 7.15HH5 pKa = 6.66ARR7 pKa = 11.84EE8 pKa = 4.0EE9 pKa = 3.88LAQGVGHH16 pKa = 6.91PPRR19 pKa = 11.84CEE21 pKa = 4.1SEE23 pKa = 5.14LSLQRR28 pKa = 11.84PFLGPEE34 pKa = 3.46EE35 pKa = 4.67DD36 pKa = 3.6AQFTLKK42 pKa = 10.35FHH44 pKa = 7.3RR45 pKa = 11.84EE46 pKa = 4.04VLDD49 pKa = 4.06SFQTSQKK56 pKa = 10.19VKK58 pKa = 10.1PGWRR62 pKa = 11.84CAPLADD68 pKa = 4.06RR69 pKa = 11.84EE70 pKa = 4.44GRR72 pKa = 11.84RR73 pKa = 11.84GNPDD77 pKa = 2.64VRR79 pKa = 11.84VFSGHH84 pKa = 5.89SLPGYY89 pKa = 9.83RR90 pKa = 11.84PADD93 pKa = 3.84PFPAARR99 pKa = 11.84GPLEE103 pKa = 4.05VLPLAVQKK111 pKa = 9.41PCCEE115 pKa = 4.03AVSQPLGQRR124 pKa = 11.84HH125 pKa = 5.27AAHH128 pKa = 7.14RR129 pKa = 11.84SSQRR133 pKa = 11.84RR134 pKa = 11.84QAISPDD140 pKa = 3.39YY141 pKa = 9.72QRR143 pKa = 11.84QGRR146 pKa = 11.84RR147 pKa = 11.84EE148 pKa = 3.89SSLPRR153 pKa = 11.84PAPNARR159 pKa = 11.84VEE161 pKa = 4.11DD162 pKa = 3.74AKK164 pKa = 11.17YY165 pKa = 10.79SPNSHH170 pKa = 6.69RR171 pKa = 11.84GAKK174 pKa = 9.14IHH176 pKa = 6.9GSLHH180 pKa = 6.4RR181 pKa = 11.84CRR183 pKa = 11.84QRR185 pKa = 11.84SPGRR189 pKa = 11.84SKK191 pKa = 10.84AAILGRR197 pKa = 11.84VKK199 pKa = 10.75RR200 pKa = 11.84SDD202 pKa = 3.73QPPKK206 pKa = 10.73GYY208 pKa = 10.41AADD211 pKa = 4.04GGEE214 pKa = 4.3LCLNPIPLAHH224 pKa = 6.47GRR226 pKa = 11.84LPRR229 pKa = 11.84RR230 pKa = 11.84PGNRR234 pKa = 11.84EE235 pKa = 3.84EE236 pKa = 4.06VCEE239 pKa = 3.97RR240 pKa = 11.84CSPRR244 pKa = 11.84FRR246 pKa = 11.84RR247 pKa = 11.84IHH249 pKa = 4.94EE250 pKa = 4.46TVFLLLPRR258 pKa = 11.84DD259 pKa = 3.64LKK261 pKa = 11.39DD262 pKa = 4.12LFGEE266 pKa = 4.3KK267 pKa = 10.21NVVSGSVMFTDD278 pKa = 4.35RR279 pKa = 11.84EE280 pKa = 4.06LRR282 pKa = 11.84SAGFRR287 pKa = 11.84HH288 pKa = 5.79FLVSVGHH295 pKa = 6.08FWWPDD300 pKa = 2.82RR301 pKa = 11.84RR302 pKa = 11.84KK303 pKa = 10.29VVATDD308 pKa = 3.33DD309 pKa = 3.73RR310 pKa = 11.84PGRR313 pKa = 11.84RR314 pKa = 11.84CRR316 pKa = 11.84LGGQGNVLEE325 pKa = 4.94GEE327 pKa = 4.38TPRR330 pKa = 11.84RR331 pKa = 11.84GPGRR335 pKa = 11.84LGPKK339 pKa = 8.88EE340 pKa = 3.95VNEE343 pKa = 4.18IKK345 pKa = 10.36QGPRR349 pKa = 11.84RR350 pKa = 11.84RR351 pKa = 11.84EE352 pKa = 3.8AHH354 pKa = 6.87HH355 pKa = 7.23DD356 pKa = 3.69PPPDD360 pKa = 3.78RR361 pKa = 11.84EE362 pKa = 4.31TVAIRR367 pKa = 11.84QTSIQGTCQFSEE379 pKa = 4.68DD380 pKa = 4.21LLNLCRR386 pKa = 11.84WRR388 pKa = 11.84LSRR391 pKa = 11.84TGEE394 pKa = 3.8EE395 pKa = 4.38TIDD398 pKa = 3.4KK399 pKa = 10.99GRR401 pKa = 11.84DD402 pKa = 3.2QVLRR406 pKa = 11.84EE407 pKa = 4.07QICSGSQVGGEE418 pKa = 3.92ADD420 pKa = 3.56VLLLRR425 pKa = 11.84RR426 pKa = 11.84LLTVNPLGLSPEE438 pKa = 4.34GPRR441 pKa = 11.84VIPRR445 pKa = 11.84QDD447 pKa = 3.23ALHH450 pKa = 6.65APVFPEE456 pKa = 3.86TTEE459 pKa = 3.76

Molecular weight:
51.53 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KIV3|A0A1L3KIV3_9VIRU RNA-dependent RNA polymerase OS=Wenling narna-like virus 7 OX=1923507 PE=4 SV=1
MM1 pKa = 7.57PRR3 pKa = 11.84NKK5 pKa = 9.75ARR7 pKa = 11.84HH8 pKa = 5.33RR9 pKa = 11.84WVCLYY14 pKa = 10.71EE15 pKa = 4.16LTLSVATLLPARR27 pKa = 11.84PGTGPYY33 pKa = 10.37LGGTRR38 pKa = 11.84PNGPDD43 pKa = 2.73TGRR46 pKa = 11.84FLPRR50 pKa = 11.84FPRR53 pKa = 11.84HH54 pKa = 5.44PNIRR58 pKa = 11.84CPKK61 pKa = 8.44EE62 pKa = 3.67RR63 pKa = 11.84GNAATGKK70 pKa = 5.35TTNGNVNGILSKK82 pKa = 9.92VLRR85 pKa = 11.84RR86 pKa = 11.84RR87 pKa = 11.84PLGNQRR93 pKa = 11.84WTRR96 pKa = 11.84LEE98 pKa = 4.04EE99 pKa = 3.55AWRR102 pKa = 11.84TILAAIAACGFVRR115 pKa = 11.84LDD117 pKa = 3.3SSRR120 pKa = 11.84RR121 pKa = 11.84AHH123 pKa = 6.93RR124 pKa = 11.84EE125 pKa = 3.37ALRR128 pKa = 11.84GLSRR132 pKa = 11.84LSTWIVKK139 pKa = 8.15TAALSGTEE147 pKa = 3.85EE148 pKa = 4.08VLRR151 pKa = 11.84EE152 pKa = 4.06LKK154 pKa = 9.04TWSARR159 pKa = 11.84LRR161 pKa = 11.84AHH163 pKa = 6.22VVNSLPPHH171 pKa = 5.77KK172 pKa = 9.81RR173 pKa = 11.84RR174 pKa = 11.84RR175 pKa = 11.84GFARR179 pKa = 11.84YY180 pKa = 7.98FQGVLRR186 pKa = 11.84SLIDD190 pKa = 3.36KK191 pKa = 10.42DD192 pKa = 3.95SRR194 pKa = 11.84ALQMSYY200 pKa = 10.01IGRR203 pKa = 11.84ALPVGSFRR211 pKa = 11.84EE212 pKa = 4.12TEE214 pKa = 3.75GAIRR218 pKa = 11.84KK219 pKa = 8.55FRR221 pKa = 11.84EE222 pKa = 4.01VTQVPFEE229 pKa = 4.1THH231 pKa = 7.16PDD233 pKa = 3.48LLDD236 pKa = 4.47SIRR239 pKa = 11.84DD240 pKa = 3.73WGAHH244 pKa = 3.75WAARR248 pKa = 11.84FLDD251 pKa = 4.48TPAMPPLWRR260 pKa = 11.84SSSGCVDD267 pKa = 4.23FSRR270 pKa = 11.84LQGGLAEE277 pKa = 4.33AAVRR281 pKa = 11.84AADD284 pKa = 4.26FAAAEE289 pKa = 4.3VEE291 pKa = 4.97SRR293 pKa = 11.84EE294 pKa = 4.29DD295 pKa = 3.2WDD297 pKa = 4.53DD298 pKa = 3.74LRR300 pKa = 11.84NTAAEE305 pKa = 4.28VPEE308 pKa = 4.18MTDD311 pKa = 3.06CDD313 pKa = 3.1VDD315 pKa = 3.49IAVRR319 pKa = 11.84EE320 pKa = 4.05AQLRR324 pKa = 11.84DD325 pKa = 3.34VALTVAAHH333 pKa = 6.66LPDD336 pKa = 4.66LPPVEE341 pKa = 4.5VCTVPEE347 pKa = 4.05RR348 pKa = 11.84GMKK351 pKa = 9.98CRR353 pKa = 11.84IVTKK357 pKa = 10.61APWCLIHH364 pKa = 7.1IGHH367 pKa = 7.48FLRR370 pKa = 11.84SWLFGGLRR378 pKa = 11.84KK379 pKa = 9.76DD380 pKa = 3.34RR381 pKa = 11.84RR382 pKa = 11.84VQGVLAGDD390 pKa = 3.36HH391 pKa = 6.68AGALRR396 pKa = 11.84GQTEE400 pKa = 4.01RR401 pKa = 11.84VYY403 pKa = 10.44SQKK406 pKa = 9.2PTEE409 pKa = 4.32EE410 pKa = 4.03EE411 pKa = 4.23NICLSADD418 pKa = 3.38LTAATDD424 pKa = 4.46LFPQDD429 pKa = 3.66LVAALVDD436 pKa = 4.01GLLSGARR443 pKa = 11.84KK444 pKa = 8.99PPTAEE449 pKa = 3.48IQEE452 pKa = 4.4VFRR455 pKa = 11.84KK456 pKa = 8.53LTGPLNARR464 pKa = 11.84LPDD467 pKa = 3.84GDD469 pKa = 3.64GFAIRR474 pKa = 11.84RR475 pKa = 11.84GIMMGFPTTWTLLNLVNLFWSEE497 pKa = 4.37TAWSASGSLPFQDD510 pKa = 3.8VALSAQPAASPRR522 pKa = 11.84TIVCGDD528 pKa = 3.87DD529 pKa = 4.06LASVWPPKK537 pKa = 10.3VADD540 pKa = 4.4RR541 pKa = 11.84YY542 pKa = 10.33EE543 pKa = 4.27EE544 pKa = 3.99VAEE547 pKa = 4.06ACGAKK552 pKa = 10.26FSVGKK557 pKa = 9.87HH558 pKa = 4.02YY559 pKa = 10.69RR560 pKa = 11.84SRR562 pKa = 11.84DD563 pKa = 3.49YY564 pKa = 11.22ILFTEE569 pKa = 5.21EE570 pKa = 3.45IFKK573 pKa = 10.97VSWEE577 pKa = 4.02KK578 pKa = 10.89QEE580 pKa = 3.91YY581 pKa = 10.93SLMDD585 pKa = 3.35SAKK588 pKa = 10.03PRR590 pKa = 11.84RR591 pKa = 11.84TTLADD596 pKa = 4.08FFPVPRR602 pKa = 11.84APRR605 pKa = 11.84KK606 pKa = 7.65STMRR610 pKa = 11.84KK611 pKa = 9.22RR612 pKa = 11.84YY613 pKa = 9.13RR614 pKa = 11.84VQAKK618 pKa = 8.7LAPVRR623 pKa = 11.84CVPLRR628 pKa = 11.84GLVRR632 pKa = 11.84PLHH635 pKa = 5.74APKK638 pKa = 10.29DD639 pKa = 3.68RR640 pKa = 11.84RR641 pKa = 11.84LRR643 pKa = 11.84PSWAALPAAVEE654 pKa = 4.01AAMDD658 pKa = 4.28LGAPVAVRR666 pKa = 11.84RR667 pKa = 11.84VLRR670 pKa = 11.84VLHH673 pKa = 5.96PGIWCWARR681 pKa = 11.84KK682 pKa = 9.5RR683 pKa = 11.84GFSPTLPLVVGGYY696 pKa = 9.83GLPPLRR702 pKa = 11.84GSVRR706 pKa = 11.84RR707 pKa = 11.84VSLPKK712 pKa = 8.97WLRR715 pKa = 11.84YY716 pKa = 9.37GLAAWLLNSKK726 pKa = 9.32WKK728 pKa = 10.43DD729 pKa = 3.2LEE731 pKa = 4.56GPSRR735 pKa = 11.84CWEE738 pKa = 4.41GVCRR742 pKa = 11.84PVSWQTMARR751 pKa = 11.84EE752 pKa = 4.04HH753 pKa = 6.7ADD755 pKa = 3.18VRR757 pKa = 11.84IASSTFAVRR766 pKa = 11.84KK767 pKa = 9.88GRR769 pKa = 11.84TPPPGFHH776 pKa = 7.12LLGGLEE782 pKa = 4.64AIQNLSVKK790 pKa = 10.38FEE792 pKa = 4.36GEE794 pKa = 4.01LCVLLGTEE802 pKa = 4.16EE803 pKa = 4.68GSLKK807 pKa = 10.47RR808 pKa = 11.84QFGLAPRR815 pKa = 11.84RR816 pKa = 11.84VANSLRR822 pKa = 11.84KK823 pKa = 9.23FFARR827 pKa = 11.84MVSDD831 pKa = 3.54HH832 pKa = 7.0PSRR835 pKa = 11.84RR836 pKa = 11.84PFPSSLGRR844 pKa = 11.84TALVQRR850 pKa = 11.84WRR852 pKa = 11.84SRR854 pKa = 11.84AEE856 pKa = 3.48EE857 pKa = 3.85RR858 pKa = 11.84ALYY861 pKa = 7.48STPRR865 pKa = 11.84SAVEE869 pKa = 3.89GLVLSLPVRR878 pKa = 11.84AKK880 pKa = 10.59RR881 pKa = 11.84LLAEE885 pKa = 4.09ALGWVV890 pKa = 3.71

Molecular weight:
99.4 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1349

459

890

674.5

75.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.192 ± 1.318

2.076 ± 0.247

4.448 ± 0.158

6.004 ± 0.543

3.484 ± 0.099

7.784 ± 0.527

2.52 ± 0.543

2.52 ± 0.057

3.632 ± 0.167

10.526 ± 0.631

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.038 ± 0.276

2.001 ± 0.081

8.08 ± 0.69

3.113 ± 0.87

12.305 ± 0.551

6.301 ± 0.008

4.448 ± 0.541

7.042 ± 0.332

2.224 ± 0.62

1.26 ± 0.178

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski