Hubei narna-like virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIJ9|A0A1L3KIJ9_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 5 OX=1922959 PE=4 SV=1
MM1 pKa = 7.46SISSSSNPHH10 pKa = 4.7EE11 pKa = 4.16QEE13 pKa = 3.9KK14 pKa = 11.11KK15 pKa = 11.05LMTDD19 pKa = 3.93YY20 pKa = 10.8IKK22 pKa = 10.69KK23 pKa = 10.04RR24 pKa = 11.84QDD26 pKa = 3.23FKK28 pKa = 10.96WRR30 pKa = 11.84STYY33 pKa = 10.26HH34 pKa = 4.84PQARR38 pKa = 11.84RR39 pKa = 11.84MFMLYY44 pKa = 10.46EE45 pKa = 3.95FTYY48 pKa = 10.52KK49 pKa = 10.2QFGYY53 pKa = 10.2SFEE56 pKa = 4.34PTTLDD61 pKa = 3.22QQFYY65 pKa = 10.34EE66 pKa = 4.54WMASTNRR73 pKa = 11.84LEE75 pKa = 6.12KK76 pKa = 9.88FLKK79 pKa = 9.85WKK81 pKa = 9.06TSNILNRR88 pKa = 11.84SVGSLEE94 pKa = 4.41RR95 pKa = 11.84VASPFLMHH103 pKa = 7.39NIPMIFDD110 pKa = 3.62KK111 pKa = 11.18YY112 pKa = 11.13FNDD115 pKa = 3.79TARR118 pKa = 11.84GLLSNDD124 pKa = 3.16SSLHH128 pKa = 5.06RR129 pKa = 11.84MKK131 pKa = 10.55LAKK134 pKa = 9.93RR135 pKa = 11.84NRR137 pKa = 11.84SKK139 pKa = 11.02YY140 pKa = 8.71VQFCFSIYY148 pKa = 7.41QAKK151 pKa = 9.53GASLPADD158 pKa = 3.19EE159 pKa = 5.08WKK161 pKa = 10.58IKK163 pKa = 9.37TSEE166 pKa = 4.14KK167 pKa = 7.97EE168 pKa = 4.07TCVRR172 pKa = 11.84LSKK175 pKa = 10.94EE176 pKa = 3.74KK177 pKa = 10.42FLNGTPGLPQGHH189 pKa = 7.49LMTPLRR195 pKa = 11.84IIDD198 pKa = 3.73EE199 pKa = 5.03DD200 pKa = 4.42YY201 pKa = 11.16LFSEE205 pKa = 4.12IHH207 pKa = 5.55RR208 pKa = 11.84TVDD211 pKa = 3.35EE212 pKa = 4.3IFPRR216 pKa = 11.84RR217 pKa = 11.84PPKK220 pKa = 9.92TFEE223 pKa = 5.43PITDD227 pKa = 4.07FLSNQKK233 pKa = 9.17IRR235 pKa = 11.84QINRR239 pKa = 11.84ILNQKK244 pKa = 8.37EE245 pKa = 3.73FQPRR249 pKa = 11.84SRR251 pKa = 11.84VPGRR255 pKa = 11.84GASWEE260 pKa = 4.37SNRR263 pKa = 11.84GAGGNLGYY271 pKa = 10.87LSMIGAGAIFLPYY284 pKa = 10.12DD285 pKa = 3.37YY286 pKa = 10.31LICYY290 pKa = 8.77FVGPGGVEE298 pKa = 3.87QVRR301 pKa = 11.84STLCQEE307 pKa = 4.57DD308 pKa = 3.83IEE310 pKa = 4.49YY311 pKa = 10.52GQYY314 pKa = 11.35LLRR317 pKa = 11.84QFSYY321 pKa = 10.79GQEE324 pKa = 4.26CTCAQPIGLPEE335 pKa = 4.06PFKK338 pKa = 11.32VRR340 pKa = 11.84VITKK344 pKa = 8.15GWTPVYY350 pKa = 10.02HH351 pKa = 6.47VARR354 pKa = 11.84EE355 pKa = 3.92YY356 pKa = 10.82QPYY359 pKa = 8.49IWSRR363 pKa = 11.84LKK365 pKa = 10.46QFPQFRR371 pKa = 11.84LTGEE375 pKa = 4.16QLNPAIMRR383 pKa = 11.84QFCSEE388 pKa = 3.88QDD390 pKa = 3.45PEE392 pKa = 3.93QDD394 pKa = 2.37EE395 pKa = 4.89WYY397 pKa = 10.12ISGDD401 pKa = 3.55YY402 pKa = 10.7QEE404 pKa = 5.06ATDD407 pKa = 5.16HH408 pKa = 6.51IPSIFAEE415 pKa = 4.76RR416 pKa = 11.84ILEE419 pKa = 4.3RR420 pKa = 11.84ICQNLRR426 pKa = 11.84IPTEE430 pKa = 4.81DD431 pKa = 3.69IPCLLASLTRR441 pKa = 11.84HH442 pKa = 6.92LIVQDD447 pKa = 2.99GDD449 pKa = 3.93VYY451 pKa = 10.62HH452 pKa = 6.11QRR454 pKa = 11.84SGQLMGSPTSFYY466 pKa = 10.71ILCIYY471 pKa = 10.17NAILTRR477 pKa = 11.84IAIEE481 pKa = 3.75NAEE484 pKa = 4.38EE485 pKa = 4.29GRR487 pKa = 11.84HH488 pKa = 4.79IKK490 pKa = 10.71YY491 pKa = 9.67PLSEE495 pKa = 4.58LPVLINGDD503 pKa = 3.9DD504 pKa = 3.7LLFRR508 pKa = 11.84AKK510 pKa = 9.63LTTFLTWKK518 pKa = 10.59SVVNWGGLIPSVGKK532 pKa = 8.25TLVSKK537 pKa = 10.94RR538 pKa = 11.84FGSINSRR545 pKa = 11.84IFRR548 pKa = 11.84FDD550 pKa = 2.57TRR552 pKa = 11.84YY553 pKa = 9.94FKK555 pKa = 9.51NTKK558 pKa = 10.24YY559 pKa = 10.53IDD561 pKa = 3.58ATPINHH567 pKa = 6.3IQLALAHH574 pKa = 6.57GSMKK578 pKa = 10.55NGNVSDD584 pKa = 3.51EE585 pKa = 4.41HH586 pKa = 6.32TNFMSKK592 pKa = 10.28QIRR595 pKa = 11.84FQEE598 pKa = 4.48FMDD601 pKa = 3.74SCPNKK606 pKa = 9.94SRR608 pKa = 11.84AWKK611 pKa = 10.27YY612 pKa = 10.7LFSLHH617 pKa = 6.38QDD619 pKa = 3.47YY620 pKa = 11.46LSIHH624 pKa = 6.65CKK626 pKa = 10.12NFPLASLCLPLEE638 pKa = 4.4LGGLGFPSPPKK649 pKa = 10.4DD650 pKa = 3.1SVYY653 pKa = 9.79YY654 pKa = 10.48AKK656 pKa = 10.58RR657 pKa = 11.84EE658 pKa = 3.95PRR660 pKa = 11.84PLSLILARR668 pKa = 11.84MIMDD672 pKa = 3.51HH673 pKa = 6.3TEE675 pKa = 3.69MGDD678 pKa = 3.23RR679 pKa = 11.84GALRR683 pKa = 11.84DD684 pKa = 3.28WRR686 pKa = 11.84LLVRR690 pKa = 11.84TGEE693 pKa = 4.29VPANCAEE700 pKa = 4.24LLLFDD705 pKa = 4.66RR706 pKa = 11.84QSEE709 pKa = 4.7YY710 pKa = 10.52CQKK713 pKa = 9.65SQCPILVRR721 pKa = 11.84PKK723 pKa = 10.29EE724 pKa = 4.22EE725 pKa = 4.1KK726 pKa = 10.11TILPPSEE733 pKa = 3.93LSYY736 pKa = 11.48GVGLTNTFDD745 pKa = 3.75HH746 pKa = 7.4FDD748 pKa = 3.92TISNVMSRR756 pKa = 11.84RR757 pKa = 11.84KK758 pKa = 9.42SLHH761 pKa = 5.25YY762 pKa = 10.39KK763 pKa = 9.78ILNSLKK769 pKa = 10.37KK770 pKa = 7.95YY771 pKa = 7.31TKK773 pKa = 10.01SGRR776 pKa = 11.84GPCKK780 pKa = 9.69ISVVNSFIRR789 pKa = 11.84SSAWRR794 pKa = 11.84FCYY797 pKa = 9.93GKK799 pKa = 10.68YY800 pKa = 10.24SSCLKK805 pKa = 9.92IDD807 pKa = 3.33NGFISFPP814 pKa = 3.91

Molecular weight: 94.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIJ9|A0A1L3KIJ9_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 5 OX=1922959 PE=4 SV=1
MM1 pKa = 7.46SISSSSNPHH10 pKa = 4.7EE11 pKa = 4.16QEE13 pKa = 3.9KK14 pKa = 11.11KK15 pKa = 11.05LMTDD19 pKa = 3.93YY20 pKa = 10.8IKK22 pKa = 10.69KK23 pKa = 10.04RR24 pKa = 11.84QDD26 pKa = 3.23FKK28 pKa = 10.96WRR30 pKa = 11.84STYY33 pKa = 10.26HH34 pKa = 4.84PQARR38 pKa = 11.84RR39 pKa = 11.84MFMLYY44 pKa = 10.46EE45 pKa = 3.95FTYY48 pKa = 10.52KK49 pKa = 10.2QFGYY53 pKa = 10.2SFEE56 pKa = 4.34PTTLDD61 pKa = 3.22QQFYY65 pKa = 10.34EE66 pKa = 4.54WMASTNRR73 pKa = 11.84LEE75 pKa = 6.12KK76 pKa = 9.88FLKK79 pKa = 9.85WKK81 pKa = 9.06TSNILNRR88 pKa = 11.84SVGSLEE94 pKa = 4.41RR95 pKa = 11.84VASPFLMHH103 pKa = 7.39NIPMIFDD110 pKa = 3.62KK111 pKa = 11.18YY112 pKa = 11.13FNDD115 pKa = 3.79TARR118 pKa = 11.84GLLSNDD124 pKa = 3.16SSLHH128 pKa = 5.06RR129 pKa = 11.84MKK131 pKa = 10.55LAKK134 pKa = 9.93RR135 pKa = 11.84NRR137 pKa = 11.84SKK139 pKa = 11.02YY140 pKa = 8.71VQFCFSIYY148 pKa = 7.41QAKK151 pKa = 9.53GASLPADD158 pKa = 3.19EE159 pKa = 5.08WKK161 pKa = 10.58IKK163 pKa = 9.37TSEE166 pKa = 4.14KK167 pKa = 7.97EE168 pKa = 4.07TCVRR172 pKa = 11.84LSKK175 pKa = 10.94EE176 pKa = 3.74KK177 pKa = 10.42FLNGTPGLPQGHH189 pKa = 7.49LMTPLRR195 pKa = 11.84IIDD198 pKa = 3.73EE199 pKa = 5.03DD200 pKa = 4.42YY201 pKa = 11.16LFSEE205 pKa = 4.12IHH207 pKa = 5.55RR208 pKa = 11.84TVDD211 pKa = 3.35EE212 pKa = 4.3IFPRR216 pKa = 11.84RR217 pKa = 11.84PPKK220 pKa = 9.92TFEE223 pKa = 5.43PITDD227 pKa = 4.07FLSNQKK233 pKa = 9.17IRR235 pKa = 11.84QINRR239 pKa = 11.84ILNQKK244 pKa = 8.37EE245 pKa = 3.73FQPRR249 pKa = 11.84SRR251 pKa = 11.84VPGRR255 pKa = 11.84GASWEE260 pKa = 4.37SNRR263 pKa = 11.84GAGGNLGYY271 pKa = 10.87LSMIGAGAIFLPYY284 pKa = 10.12DD285 pKa = 3.37YY286 pKa = 10.31LICYY290 pKa = 8.77FVGPGGVEE298 pKa = 3.87QVRR301 pKa = 11.84STLCQEE307 pKa = 4.57DD308 pKa = 3.83IEE310 pKa = 4.49YY311 pKa = 10.52GQYY314 pKa = 11.35LLRR317 pKa = 11.84QFSYY321 pKa = 10.79GQEE324 pKa = 4.26CTCAQPIGLPEE335 pKa = 4.06PFKK338 pKa = 11.32VRR340 pKa = 11.84VITKK344 pKa = 8.15GWTPVYY350 pKa = 10.02HH351 pKa = 6.47VARR354 pKa = 11.84EE355 pKa = 3.92YY356 pKa = 10.82QPYY359 pKa = 8.49IWSRR363 pKa = 11.84LKK365 pKa = 10.46QFPQFRR371 pKa = 11.84LTGEE375 pKa = 4.16QLNPAIMRR383 pKa = 11.84QFCSEE388 pKa = 3.88QDD390 pKa = 3.45PEE392 pKa = 3.93QDD394 pKa = 2.37EE395 pKa = 4.89WYY397 pKa = 10.12ISGDD401 pKa = 3.55YY402 pKa = 10.7QEE404 pKa = 5.06ATDD407 pKa = 5.16HH408 pKa = 6.51IPSIFAEE415 pKa = 4.76RR416 pKa = 11.84ILEE419 pKa = 4.3RR420 pKa = 11.84ICQNLRR426 pKa = 11.84IPTEE430 pKa = 4.81DD431 pKa = 3.69IPCLLASLTRR441 pKa = 11.84HH442 pKa = 6.92LIVQDD447 pKa = 2.99GDD449 pKa = 3.93VYY451 pKa = 10.62HH452 pKa = 6.11QRR454 pKa = 11.84SGQLMGSPTSFYY466 pKa = 10.71ILCIYY471 pKa = 10.17NAILTRR477 pKa = 11.84IAIEE481 pKa = 3.75NAEE484 pKa = 4.38EE485 pKa = 4.29GRR487 pKa = 11.84HH488 pKa = 4.79IKK490 pKa = 10.71YY491 pKa = 9.67PLSEE495 pKa = 4.58LPVLINGDD503 pKa = 3.9DD504 pKa = 3.7LLFRR508 pKa = 11.84AKK510 pKa = 9.63LTTFLTWKK518 pKa = 10.59SVVNWGGLIPSVGKK532 pKa = 8.25TLVSKK537 pKa = 10.94RR538 pKa = 11.84FGSINSRR545 pKa = 11.84IFRR548 pKa = 11.84FDD550 pKa = 2.57TRR552 pKa = 11.84YY553 pKa = 9.94FKK555 pKa = 9.51NTKK558 pKa = 10.24YY559 pKa = 10.53IDD561 pKa = 3.58ATPINHH567 pKa = 6.3IQLALAHH574 pKa = 6.57GSMKK578 pKa = 10.55NGNVSDD584 pKa = 3.51EE585 pKa = 4.41HH586 pKa = 6.32TNFMSKK592 pKa = 10.28QIRR595 pKa = 11.84FQEE598 pKa = 4.48FMDD601 pKa = 3.74SCPNKK606 pKa = 9.94SRR608 pKa = 11.84AWKK611 pKa = 10.27YY612 pKa = 10.7LFSLHH617 pKa = 6.38QDD619 pKa = 3.47YY620 pKa = 11.46LSIHH624 pKa = 6.65CKK626 pKa = 10.12NFPLASLCLPLEE638 pKa = 4.4LGGLGFPSPPKK649 pKa = 10.4DD650 pKa = 3.1SVYY653 pKa = 9.79YY654 pKa = 10.48AKK656 pKa = 10.58RR657 pKa = 11.84EE658 pKa = 3.95PRR660 pKa = 11.84PLSLILARR668 pKa = 11.84MIMDD672 pKa = 3.51HH673 pKa = 6.3TEE675 pKa = 3.69MGDD678 pKa = 3.23RR679 pKa = 11.84GALRR683 pKa = 11.84DD684 pKa = 3.28WRR686 pKa = 11.84LLVRR690 pKa = 11.84TGEE693 pKa = 4.29VPANCAEE700 pKa = 4.24LLLFDD705 pKa = 4.66RR706 pKa = 11.84QSEE709 pKa = 4.7YY710 pKa = 10.52CQKK713 pKa = 9.65SQCPILVRR721 pKa = 11.84PKK723 pKa = 10.29EE724 pKa = 4.22EE725 pKa = 4.1KK726 pKa = 10.11TILPPSEE733 pKa = 3.93LSYY736 pKa = 11.48GVGLTNTFDD745 pKa = 3.75HH746 pKa = 7.4FDD748 pKa = 3.92TISNVMSRR756 pKa = 11.84RR757 pKa = 11.84KK758 pKa = 9.42SLHH761 pKa = 5.25YY762 pKa = 10.39KK763 pKa = 9.78ILNSLKK769 pKa = 10.37KK770 pKa = 7.95YY771 pKa = 7.31TKK773 pKa = 10.01SGRR776 pKa = 11.84GPCKK780 pKa = 9.69ISVVNSFIRR789 pKa = 11.84SSAWRR794 pKa = 11.84FCYY797 pKa = 9.93GKK799 pKa = 10.68YY800 pKa = 10.24SSCLKK805 pKa = 9.92IDD807 pKa = 3.33NGFISFPP814 pKa = 3.91

Molecular weight: 94.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

814

814

814

814.0

94.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.054 ± 0.0

2.334 ± 0.0

4.3 ± 0.0

5.651 ± 0.0

5.651 ± 0.0

5.774 ± 0.0

2.334 ± 0.0

6.88 ± 0.0

6.143 ± 0.0

9.582 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.334 ± 0.0

4.177 ± 0.0

5.897 ± 0.0

4.668 ± 0.0

7.002 ± 0.0

8.477 ± 0.0

4.914 ± 0.0

3.563 ± 0.0

1.597 ± 0.0

4.668 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski