Amino acid dipepetide frequency for Apis cerana cerana (Oriental honeybee)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.308AlaAla: 4.308 ± 0.049	1.076AlaCys: 1.076 ± 0.014	2.478AlaAsp: 2.478 ± 0.025	3.53AlaGlu: 3.53 ± 0.038	2.034AlaPhe: 2.034 ± 0.021	3.019AlaGly: 3.019 ± 0.032	1.215AlaHis: 1.215 ± 0.019	3.668AlaIle: 3.668 ± 0.031	3.443AlaLys: 3.443 ± 0.03	5.393AlaLeu: 5.393 ± 0.037	1.39AlaMet: 1.39 ± 0.017	2.534AlaAsn: 2.534 ± 0.022	2.311AlaPro: 2.311 ± 0.029	2.192AlaGln: 2.192 ± 0.023	2.949AlaArg: 2.949 ± 0.03	4.239AlaSer: 4.239 ± 0.036	3.474AlaThr: 3.474 ± 0.03	3.416AlaVal: 3.416 ± 0.029	0.585AlaTrp: 0.585 ± 0.012	1.557AlaTyr: 1.557 ± 0.016	0.0AlaXaa: 0.0 ± 0.0
Cys	1.027CysAla: 1.027 ± 0.016	0.474CysCys: 0.474 ± 0.012	1.097CysAsp: 1.097 ± 0.018	1.171CysGlu: 1.171 ± 0.022	0.74CysPhe: 0.74 ± 0.012	1.247CysGly: 1.247 ± 0.02	0.5CysHis: 0.5 ± 0.012	1.325CysIle: 1.325 ± 0.02	1.258CysLys: 1.258 ± 0.022	1.827CysLeu: 1.827 ± 0.023	0.414CysMet: 0.414 ± 0.01	1.095CysAsn: 1.095 ± 0.019	0.999CysPro: 0.999 ± 0.023	0.772CysGln: 0.772 ± 0.016	1.022CysArg: 1.022 ± 0.017	1.574CysSer: 1.574 ± 0.025	1.138CysThr: 1.138 ± 0.016	1.1CysVal: 1.1 ± 0.018	0.226CysTrp: 0.226 ± 0.006	0.641CysTyr: 0.641 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	2.637AspAla: 2.637 ± 0.024	0.963AspCys: 0.963 ± 0.016	3.358AspAsp: 3.358 ± 0.038	4.132AspGlu: 4.132 ± 0.039	2.075AspPhe: 2.075 ± 0.02	2.808AspGly: 2.808 ± 0.03	1.078AspHis: 1.078 ± 0.017	4.143AspIle: 4.143 ± 0.033	3.47AspLys: 3.47 ± 0.032	4.727AspLeu: 4.727 ± 0.031	1.2AspMet: 1.2 ± 0.017	2.925AspAsn: 2.925 ± 0.029	2.287AspPro: 2.287 ± 0.023	1.752AspGln: 1.752 ± 0.02	2.536AspArg: 2.536 ± 0.033	4.154AspSer: 4.154 ± 0.037	2.937AspThr: 2.937 ± 0.026	3.228AspVal: 3.228 ± 0.027	0.604AspTrp: 0.604 ± 0.011	1.838AspTyr: 1.838 ± 0.02	0.0AspXaa: 0.0 ± 0.0
Glu	3.715GluAla: 3.715 ± 0.035	1.27GluCys: 1.27 ± 0.028	4.212GluAsp: 4.212 ± 0.034	7.091GluGlu: 7.091 ± 0.1	2.28GluPhe: 2.28 ± 0.022	2.926GluGly: 2.926 ± 0.031	1.487GluHis: 1.487 ± 0.017	4.972GluIle: 4.972 ± 0.045	6.091GluLys: 6.091 ± 0.062	6.05GluLeu: 6.05 ± 0.052	1.647GluMet: 1.647 ± 0.021	4.768GluAsn: 4.768 ± 0.045	2.36GluPro: 2.36 ± 0.029	2.928GluGln: 2.928 ± 0.032	3.779GluArg: 3.779 ± 0.047	4.786GluSer: 4.786 ± 0.042	3.943GluThr: 3.943 ± 0.032	3.511GluVal: 3.511 ± 0.028	0.709GluTrp: 0.709 ± 0.013	2.194GluTyr: 2.194 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	1.928PheAla: 1.928 ± 0.019	0.793PheCys: 0.793 ± 0.014	2.048PheAsp: 2.048 ± 0.019	2.273PheGlu: 2.273 ± 0.023	1.492PhePhe: 1.492 ± 0.02	2.162PheGly: 2.162 ± 0.027	0.973PheHis: 0.973 ± 0.014	2.396PheIle: 2.396 ± 0.028	2.294PheLys: 2.294 ± 0.026	3.653PheLeu: 3.653 ± 0.029	0.823PheMet: 0.823 ± 0.011	1.977PheAsn: 1.977 ± 0.02	1.594PhePro: 1.594 ± 0.018	1.469PheGln: 1.469 ± 0.015	1.765PheArg: 1.765 ± 0.021	2.876PheSer: 2.876 ± 0.023	2.076PheThr: 2.076 ± 0.021	2.233PheVal: 2.233 ± 0.023	0.424PheTrp: 0.424 ± 0.011	1.348PheTyr: 1.348 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	2.758GlyAla: 2.758 ± 0.036	0.974GlyCys: 0.974 ± 0.018	2.562GlyAsp: 2.562 ± 0.027	3.026GlyGlu: 3.026 ± 0.03	2.048GlyPhe: 2.048 ± 0.025	3.862GlyGly: 3.862 ± 0.058	1.277GlyHis: 1.277 ± 0.019	3.513GlyIle: 3.513 ± 0.03	3.417GlyLys: 3.417 ± 0.03	4.279GlyLeu: 4.279 ± 0.035	1.144GlyMet: 1.144 ± 0.016	2.759GlyAsn: 2.759 ± 0.026	2.188GlyPro: 2.188 ± 0.042	1.97GlyGln: 1.97 ± 0.029	2.751GlyArg: 2.751 ± 0.029	4.296GlySer: 4.296 ± 0.042	3.109GlyThr: 3.109 ± 0.03	2.845GlyVal: 2.845 ± 0.03	0.622GlyTrp: 0.622 ± 0.013	1.877GlyTyr: 1.877 ± 0.027	0.0GlyXaa: 0.0 ± 0.0
His	1.236HisAla: 1.236 ± 0.016	0.573HisCys: 0.573 ± 0.011	1.06HisAsp: 1.06 ± 0.014	1.405HisGlu: 1.405 ± 0.017	0.941HisPhe: 0.941 ± 0.014	1.298HisGly: 1.298 ± 0.016	1.025HisHis: 1.025 ± 0.031	1.582HisIle: 1.582 ± 0.018	1.426HisLys: 1.426 ± 0.014	2.308HisLeu: 2.308 ± 0.023	0.585HisMet: 0.585 ± 0.011	1.244HisAsn: 1.244 ± 0.018	1.27HisPro: 1.27 ± 0.019	1.125HisGln: 1.125 ± 0.018	1.4HisArg: 1.4 ± 0.019	1.887HisSer: 1.887 ± 0.023	1.326HisThr: 1.326 ± 0.017	1.401HisVal: 1.401 ± 0.015	0.295HisTrp: 0.295 ± 0.008	0.863HisTyr: 0.863 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	3.768IleAla: 3.768 ± 0.029	1.466IleCys: 1.466 ± 0.024	3.762IleAsp: 3.762 ± 0.032	4.828IleGlu: 4.828 ± 0.05	2.692IlePhe: 2.692 ± 0.031	3.255IleGly: 3.255 ± 0.032	1.613IleHis: 1.613 ± 0.016	4.679IleIle: 4.679 ± 0.047	4.772IleLys: 4.772 ± 0.042	6.456IleLeu: 6.456 ± 0.052	1.41IleMet: 1.41 ± 0.018	3.95IleAsn: 3.95 ± 0.045	3.28IlePro: 3.28 ± 0.027	2.844IleGln: 2.844 ± 0.027	3.051IleArg: 3.051 ± 0.024	5.439IleSer: 5.439 ± 0.039	3.923IleThr: 3.923 ± 0.033	3.857IleVal: 3.857 ± 0.032	0.677IleTrp: 0.677 ± 0.013	2.16IleTyr: 2.16 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	3.178LysAla: 3.178 ± 0.03	1.324LysCys: 1.324 ± 0.021	3.799LysAsp: 3.799 ± 0.036	5.803LysGlu: 5.803 ± 0.062	2.375LysPhe: 2.375 ± 0.025	2.669LysGly: 2.669 ± 0.028	1.633LysHis: 1.633 ± 0.021	5.157LysIle: 5.157 ± 0.051	6.395LysLys: 6.395 ± 0.077	6.33LysLeu: 6.33 ± 0.048	1.646LysMet: 1.646 ± 0.019	4.52LysAsn: 4.52 ± 0.048	2.768LysPro: 2.768 ± 0.026	3.028LysGln: 3.028 ± 0.028	3.883LysArg: 3.883 ± 0.034	5.004LysSer: 5.004 ± 0.04	3.779LysThr: 3.779 ± 0.03	3.475LysVal: 3.475 ± 0.026	0.714LysTrp: 0.714 ± 0.011	2.516LysTyr: 2.516 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	5.431LeuAla: 5.431 ± 0.038	1.816LeuCys: 1.816 ± 0.024	4.735LeuAsp: 4.735 ± 0.031	6.516LeuGlu: 6.516 ± 0.047	3.209LeuPhe: 3.209 ± 0.032	4.284LeuGly: 4.284 ± 0.031	2.401LeuHis: 2.401 ± 0.023	5.458LeuIle: 5.458 ± 0.046	6.663LeuLys: 6.663 ± 0.057	8.813LeuLeu: 8.813 ± 0.068	2.024LeuMet: 2.024 ± 0.021	4.924LeuAsn: 4.924 ± 0.043	4.536LeuPro: 4.536 ± 0.037	4.535LeuGln: 4.535 ± 0.037	5.056LeuArg: 5.056 ± 0.042	7.122LeuSer: 7.122 ± 0.046	5.072LeuThr: 5.072 ± 0.037	4.628LeuVal: 4.628 ± 0.037	0.943LeuTrp: 0.943 ± 0.015	2.911LeuTyr: 2.911 ± 0.028	0.0LeuXaa: 0.0 ± 0.0
Met	1.48MetAla: 1.48 ± 0.018	0.423MetCys: 0.423 ± 0.01	1.323MetAsp: 1.323 ± 0.015	1.78MetGlu: 1.78 ± 0.019	0.838MetPhe: 0.838 ± 0.013	1.089MetGly: 1.089 ± 0.016	0.52MetHis: 0.52 ± 0.009	1.412MetIle: 1.412 ± 0.018	1.711MetLys: 1.711 ± 0.021	2.009MetLeu: 2.009 ± 0.021	0.592MetMet: 0.592 ± 0.011	1.258MetAsn: 1.258 ± 0.016	1.014MetPro: 1.014 ± 0.014	1.05MetGln: 1.05 ± 0.017	1.092MetArg: 1.092 ± 0.014	1.721MetSer: 1.721 ± 0.021	1.263MetThr: 1.263 ± 0.016	1.178MetVal: 1.178 ± 0.014	0.25MetTrp: 0.25 ± 0.006	0.722MetTyr: 0.722 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.779AsnAla: 2.779 ± 0.024	1.011AsnCys: 1.011 ± 0.02	3.077AsnAsp: 3.077 ± 0.033	4.115AsnGlu: 4.115 ± 0.04	2.149AsnPhe: 2.149 ± 0.023	2.937AsnGly: 2.937 ± 0.03	1.241AsnHis: 1.241 ± 0.014	4.875AsnIle: 4.875 ± 0.054	4.025AsnLys: 4.025 ± 0.042	5.079AsnLeu: 5.079 ± 0.039	1.312AsnMet: 1.312 ± 0.019	4.331AsnAsn: 4.331 ± 0.05	2.282AsnPro: 2.282 ± 0.023	2.212AsnGln: 2.212 ± 0.028	2.463AsnArg: 2.463 ± 0.02	4.587AsnSer: 4.587 ± 0.04	3.297AsnThr: 3.297 ± 0.031	3.506AsnVal: 3.506 ± 0.029	0.57AsnTrp: 0.57 ± 0.011	1.923AsnTyr: 1.923 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	2.538ProAla: 2.538 ± 0.033	0.796ProCys: 0.796 ± 0.021	2.301ProAsp: 2.301 ± 0.024	3.096ProGlu: 3.096 ± 0.034	1.573ProPhe: 1.573 ± 0.018	2.587ProGly: 2.587 ± 0.062	1.125ProHis: 1.125 ± 0.015	2.957ProIle: 2.957 ± 0.026	2.678ProLys: 2.678 ± 0.028	4.003ProLeu: 4.003 ± 0.033	0.946ProMet: 0.946 ± 0.014	2.313ProAsn: 2.313 ± 0.025	3.881ProPro: 3.881 ± 0.075	1.999ProGln: 1.999 ± 0.03	2.402ProArg: 2.402 ± 0.032	3.997ProSer: 3.997 ± 0.04	2.907ProThr: 2.907 ± 0.03	2.837ProVal: 2.837 ± 0.033	0.496ProTrp: 0.496 ± 0.011	1.495ProTyr: 1.495 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	2.247GlnAla: 2.247 ± 0.024	0.822GlnCys: 0.822 ± 0.017	1.978GlnAsp: 1.978 ± 0.018	3.141GlnGlu: 3.141 ± 0.031	1.44GlnPhe: 1.44 ± 0.018	1.815GlnGly: 1.815 ± 0.024	1.183GlnHis: 1.183 ± 0.019	2.751GlnIle: 2.751 ± 0.027	2.927GlnLys: 2.927 ± 0.033	3.991GlnLeu: 3.991 ± 0.038	0.999GlnMet: 0.999 ± 0.018	2.68GlnAsn: 2.68 ± 0.036	1.904GlnPro: 1.904 ± 0.029	3.625GlnGln: 3.625 ± 0.092	2.348GlnArg: 2.348 ± 0.028	3.113GlnSer: 3.113 ± 0.03	2.438GlnThr: 2.438 ± 0.026	2.172GlnVal: 2.172 ± 0.024	0.47GlnTrp: 0.47 ± 0.009	1.395GlnTyr: 1.395 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	2.737ArgAla: 2.737 ± 0.025	1.021ArgCys: 1.021 ± 0.02	2.742ArgAsp: 2.742 ± 0.035	3.542ArgGlu: 3.542 ± 0.038	1.845ArgPhe: 1.845 ± 0.019	2.668ArgGly: 2.668 ± 0.032	1.421ArgHis: 1.421 ± 0.016	3.252ArgIle: 3.252 ± 0.028	3.948ArgLys: 3.948 ± 0.033	4.703ArgLeu: 4.703 ± 0.038	1.161ArgMet: 1.161 ± 0.014	2.919ArgAsn: 2.919 ± 0.026	2.23ArgPro: 2.23 ± 0.024	2.221ArgGln: 2.221 ± 0.023	3.797ArgArg: 3.797 ± 0.048	4.046ArgSer: 4.046 ± 0.046	2.671ArgThr: 2.671 ± 0.025	2.659ArgVal: 2.659 ± 0.026	0.56ArgTrp: 0.56 ± 0.012	1.758ArgTyr: 1.758 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	3.988SerAla: 3.988 ± 0.039	1.477SerCys: 1.477 ± 0.02	4.117SerAsp: 4.117 ± 0.032	4.973SerGlu: 4.973 ± 0.041	2.736SerPhe: 2.736 ± 0.026	4.425SerGly: 4.425 ± 0.043	1.801SerHis: 1.801 ± 0.021	5.052SerIle: 5.052 ± 0.04	5.111SerLys: 5.111 ± 0.042	7.033SerLeu: 7.033 ± 0.045	1.797SerMet: 1.797 ± 0.018	4.8SerAsn: 4.8 ± 0.047	4.18SerPro: 4.18 ± 0.051	3.282SerGln: 3.282 ± 0.036	3.989SerArg: 3.989 ± 0.047	8.401SerSer: 8.401 ± 0.077	5.295SerThr: 5.295 ± 0.046	4.351SerVal: 4.351 ± 0.032	0.831SerTrp: 0.831 ± 0.011	2.339SerTyr: 2.339 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	3.332ThrAla: 3.332 ± 0.031	1.185ThrCys: 1.185 ± 0.024	2.894ThrAsp: 2.894 ± 0.027	3.858ThrGlu: 3.858 ± 0.037	2.193ThrPhe: 2.193 ± 0.023	3.148ThrGly: 3.148 ± 0.026	1.232ThrHis: 1.232 ± 0.013	4.103ThrIle: 4.103 ± 0.034	3.746ThrLys: 3.746 ± 0.031	5.29ThrLeu: 5.29 ± 0.036	1.343ThrMet: 1.343 ± 0.019	3.324ThrAsn: 3.324 ± 0.032	3.095ThrPro: 3.095 ± 0.034	2.151ThrGln: 2.151 ± 0.026	2.625ThrArg: 2.625 ± 0.024	5.204ThrSer: 5.204 ± 0.048	4.247ThrThr: 4.247 ± 0.057	3.554ThrVal: 3.554 ± 0.028	0.624ThrTrp: 0.624 ± 0.013	1.77ThrTyr: 1.77 ± 0.018	0.0ThrXaa: 0.0 ± 0.0
Val	3.498ValAla: 3.498 ± 0.029	1.19ValCys: 1.19 ± 0.019	2.949ValAsp: 2.949 ± 0.028	3.733ValGlu: 3.733 ± 0.035	2.073ValPhe: 2.073 ± 0.02	2.768ValGly: 2.768 ± 0.033	1.383ValHis: 1.383 ± 0.017	3.636ValIle: 3.636 ± 0.033	3.622ValLys: 3.622 ± 0.032	5.055ValLeu: 5.055 ± 0.034	1.255ValMet: 1.255 ± 0.016	2.861ValAsn: 2.861 ± 0.025	2.891ValPro: 2.891 ± 0.032	2.446ValGln: 2.446 ± 0.024	2.673ValArg: 2.673 ± 0.027	4.338ValSer: 4.338 ± 0.035	3.59ValThr: 3.59 ± 0.028	3.396ValVal: 3.396 ± 0.032	0.624ValTrp: 0.624 ± 0.012	1.739ValTyr: 1.739 ± 0.021	0.0ValXaa: 0.0 ± 0.0
Trp	0.537TrpAla: 0.537 ± 0.011	0.226TrpCys: 0.226 ± 0.007	0.572TrpAsp: 0.572 ± 0.011	0.634TrpGlu: 0.634 ± 0.01	0.451TrpPhe: 0.451 ± 0.011	0.526TrpGly: 0.526 ± 0.01	0.272TrpHis: 0.272 ± 0.007	0.778TrpIle: 0.778 ± 0.014	0.81TrpLys: 0.81 ± 0.015	1.071TrpLeu: 1.071 ± 0.018	0.279TrpMet: 0.279 ± 0.008	0.671TrpAsn: 0.671 ± 0.011	0.409TrpPro: 0.409 ± 0.01	0.462TrpGln: 0.462 ± 0.01	0.609TrpArg: 0.609 ± 0.01	0.8TrpSer: 0.8 ± 0.014	0.606TrpThr: 0.606 ± 0.011	0.512TrpVal: 0.512 ± 0.011	0.179TrpTrp: 0.179 ± 0.007	0.38TrpTyr: 0.38 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.688TyrAla: 1.688 ± 0.019	0.767TyrCys: 0.767 ± 0.014	1.788TyrAsp: 1.788 ± 0.021	2.076TyrGlu: 2.076 ± 0.021	1.417TyrPhe: 1.417 ± 0.019	1.786TyrGly: 1.786 ± 0.022	0.87TyrHis: 0.87 ± 0.014	2.171TyrIle: 2.171 ± 0.029	2.187TyrLys: 2.187 ± 0.024	3.008TyrLeu: 3.008 ± 0.028	0.757TyrMet: 0.757 ± 0.012	1.916TyrAsn: 1.916 ± 0.022	1.447TyrPro: 1.447 ± 0.024	1.373TyrGln: 1.373 ± 0.019	1.711TyrArg: 1.711 ± 0.018	2.393TyrSer: 2.393 ± 0.025	1.83TyrThr: 1.83 ± 0.02	1.86TyrVal: 1.86 ± 0.022	0.379TyrTrp: 0.379 ± 0.01	1.309TyrTyr: 1.309 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9931 proteins (5621110 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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