Methanosphaera sp. rholeuAM270

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanosphaera; unclassified Methanosphaera

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1611 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328SDB8|A0A328SDB8_9EURY Adenylosuccinate lyase OS=Methanosphaera sp. rholeuAM270 OX=1945577 GN=BZ138_04785 PE=3 SV=1
QQ1 pKa = 7.6APNTNSAKK9 pKa = 8.25MTYY12 pKa = 10.61ASDD15 pKa = 3.94PMDD18 pKa = 3.55TSATEE23 pKa = 3.85EE24 pKa = 4.27TVAATASAYY33 pKa = 10.43AYY35 pKa = 10.59VLEE38 pKa = 4.54IDD40 pKa = 4.44KK41 pKa = 11.28ADD43 pKa = 3.87TQTGQALDD51 pKa = 3.74GVVFTLQRR59 pKa = 11.84SDD61 pKa = 3.24GYY63 pKa = 11.3YY64 pKa = 8.79VTSTGALVAADD75 pKa = 4.18ASYY78 pKa = 10.71IAADD82 pKa = 4.03GTVSAAGEE90 pKa = 3.91AAGIALTTANGGVAVVAGLDD110 pKa = 3.21AGSYY114 pKa = 10.73VLTEE118 pKa = 4.23CDD120 pKa = 3.37HH121 pKa = 5.76NASYY125 pKa = 11.44GSITPVSFDD134 pKa = 3.1IVASFAGANGAFSGLTVPATFTGGTSSLGGALVTSFEE171 pKa = 4.35SDD173 pKa = 2.87QADD176 pKa = 3.41AGKK179 pKa = 10.89VIVDD183 pKa = 3.88ISNDD187 pKa = 3.29XQIIMPLTGSNMLVMFVIAGVVLLLISAACIARR220 pKa = 11.84NRR222 pKa = 11.84RR223 pKa = 11.84KK224 pKa = 9.34DD225 pKa = 3.58TEE227 pKa = 4.16VEE229 pKa = 3.96

Molecular weight: 22.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328SIJ5|A0A328SIJ5_9EURY Peptidase M42 (Fragment) OS=Methanosphaera sp. rholeuAM270 OX=1945577 GN=BZ138_00865 PE=4 SV=1
MM1 pKa = 7.42SRR3 pKa = 11.84NKK5 pKa = 10.05NLPKK9 pKa = 10.42KK10 pKa = 10.47LRR12 pKa = 11.84LAKK15 pKa = 10.09KK16 pKa = 8.79GKK18 pKa = 7.15QNRR21 pKa = 11.84RR22 pKa = 11.84VPIFAMMKK30 pKa = 8.78TSRR33 pKa = 11.84KK34 pKa = 9.56

Molecular weight: 4.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1611

0

1611

474962

26

6722

294.8

33.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.661 ± 0.062

1.089 ± 0.034

6.199 ± 0.053

7.031 ± 0.098

3.561 ± 0.045

6.363 ± 0.061

1.625 ± 0.033

9.061 ± 0.072

7.291 ± 0.116

7.975 ± 0.101

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.156 ± 0.044

7.548 ± 0.145

2.943 ± 0.038

2.631 ± 0.045

3.241 ± 0.057

6.533 ± 0.062

7.322 ± 0.175

6.752 ± 0.101

0.62 ± 0.024

4.373 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski