CrAssphage sp.
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345BP28|A0A345BP28_9CAUD Putative tail tubular protein OS=CrAssphage sp. OX=2212563 PE=4 SV=1
MM1 pKa = 7.64 KK2 pKa = 10.25 LYY4 pKa = 10.53 KK5 pKa = 10.66 DD6 pKa = 3.73 NLGKK10 pKa = 10.53 VAITVEE16 pKa = 3.64 QGYY19 pKa = 9.4 WDD21 pKa = 3.77 EE22 pKa = 4.54 NKK24 pKa = 10.7 DD25 pKa = 3.66 YY26 pKa = 11.56 DD27 pKa = 3.87 KK28 pKa = 10.87 LTVVEE33 pKa = 4.3 RR34 pKa = 11.84 EE35 pKa = 4.69 GIFGTYY41 pKa = 9.35 ISRR44 pKa = 11.84 KK45 pKa = 7.74 PVPAGTEE52 pKa = 3.95 LSDD55 pKa = 3.18 RR56 pKa = 11.84 TYY58 pKa = 10.2 WIPFSSLKK66 pKa = 10.52 EE67 pKa = 3.95 EE68 pKa = 3.87 IVINYY73 pKa = 9.97 NEE75 pKa = 4.91 FISHH79 pKa = 6.7 YY80 pKa = 10.93 GPMLDD85 pKa = 5.08 DD86 pKa = 3.47 QAEE89 pKa = 4.31 VLNNVQLLINEE100 pKa = 5.03 IYY102 pKa = 10.72 NKK104 pKa = 9.96 LPSGIIFEE112 pKa = 4.34 GDD114 pKa = 2.92 KK115 pKa = 10.72 TFIRR119 pKa = 11.84 PGHH122 pKa = 6.12 PEE124 pKa = 4.42 EE125 pKa = 4.09 ITLTGSTVNEE135 pKa = 4.22 DD136 pKa = 3.21 PATFKK141 pKa = 10.83 LYY143 pKa = 10.76 KK144 pKa = 9.87 NSKK147 pKa = 9.35 LVHH150 pKa = 5.35 TVTAKK155 pKa = 10.48 SFTYY159 pKa = 10.21 KK160 pKa = 10.72 AIADD164 pKa = 3.98 DD165 pKa = 3.64 EE166 pKa = 4.55 CAYY169 pKa = 10.17 KK170 pKa = 10.75 LIVEE174 pKa = 4.2 QSGYY178 pKa = 10.61 RR179 pKa = 11.84 YY180 pKa = 9.31 VAGWNITLRR189 pKa = 11.84 YY190 pKa = 8.34 PIYY193 pKa = 10.17 AGSGATYY200 pKa = 9.97 EE201 pKa = 5.06 DD202 pKa = 4.06 VVSDD206 pKa = 3.68 YY207 pKa = 11.53 NIIKK211 pKa = 9.97 IDD213 pKa = 3.69 TNPEE217 pKa = 3.12 GLYY220 pKa = 10.11 IINVINDD227 pKa = 3.03 GDD229 pKa = 4.35 YY230 pKa = 11.14 IFFVAPKK237 pKa = 9.83 NVIIHH242 pKa = 5.95 SIKK245 pKa = 10.89 LNGLDD250 pKa = 3.56 VPLKK254 pKa = 8.6 QTEE257 pKa = 4.22 EE258 pKa = 4.31 VEE260 pKa = 4.8 LEE262 pKa = 4.01 TEE264 pKa = 4.24 YY265 pKa = 10.48 IIYY268 pKa = 10.59 QSDD271 pKa = 3.19 NTYY274 pKa = 10.89 EE275 pKa = 4.22 SNNYY279 pKa = 8.85 PIYY282 pKa = 9.56 INSYY286 pKa = 8.8 EE287 pKa = 4.09 GLNGGDD293 pKa = 3.49 FVKK296 pKa = 10.59 ILDD299 pKa = 3.75 VLQDD303 pKa = 3.52 KK304 pKa = 8.12 VTSEE308 pKa = 4.85 DD309 pKa = 2.65 ITRR312 pKa = 11.84 ITNEE316 pKa = 3.68 NLEE319 pKa = 4.29 IKK321 pKa = 8.9 QTLQQVKK328 pKa = 10.29 EE329 pKa = 4.25 EE330 pKa = 4.13 NNEE333 pKa = 3.75 VNNNIDD339 pKa = 3.21 AALSVVEE346 pKa = 4.55 TYY348 pKa = 8.69 KK349 pKa = 10.69 TEE351 pKa = 4.12 INKK354 pKa = 9.98 SLAIIEE360 pKa = 4.7 NISNEE365 pKa = 4.0 LNDD368 pKa = 3.02 ITEE371 pKa = 5.01 NINTSNVDD379 pKa = 3.83 LNVLKK384 pKa = 10.37 TEE386 pKa = 4.21 VTNMASSVNSLNKK399 pKa = 10.21 SLEE402 pKa = 4.03 EE403 pKa = 4.33 LGTNVYY409 pKa = 10.43 SAIDD413 pKa = 3.53 AVQEE417 pKa = 3.96 NVDD420 pKa = 4.08 RR421 pKa = 11.84 IGEE424 pKa = 4.13 GRR426 pKa = 11.84 VDD428 pKa = 4.72 IINEE432 pKa = 3.83 DD433 pKa = 3.52 GFRR436 pKa = 11.84 NNNVIYY442 pKa = 10.2 LKK444 pKa = 10.65 PNWTKK449 pKa = 10.34 VSVYY453 pKa = 10.34 GNEE456 pKa = 3.97 PEE458 pKa = 4.08 IVPVLTQNMINRR470 pKa = 11.84 PYY472 pKa = 7.32 TTYY475 pKa = 10.95 VIDD478 pKa = 3.47 YY479 pKa = 9.41 NYY481 pKa = 10.92 YY482 pKa = 9.52 IFDD485 pKa = 4.49 TIEE488 pKa = 4.13 LPIGTTLKK496 pKa = 10.36 FRR498 pKa = 11.84 AGALLFVEE506 pKa = 4.53 NGSMYY511 pKa = 10.57 SQYY514 pKa = 11.51 NCIIDD519 pKa = 4.1 DD520 pKa = 4.27 CGNNNIIQGEE530 pKa = 4.37 TLAMQIVFSNVYY542 pKa = 10.46 LDD544 pKa = 3.44 WFYY547 pKa = 11.81 LFLDD551 pKa = 3.25 IGGSYY556 pKa = 10.39 VEE558 pKa = 4.48 NLNGILDD565 pKa = 3.93 YY566 pKa = 11.11 AFSIARR572 pKa = 11.84 GPIRR576 pKa = 11.84 FRR578 pKa = 11.84 QVDD581 pKa = 3.73 RR582 pKa = 11.84 LVIDD586 pKa = 4.29 GFVNACVGTIDD597 pKa = 5.54 AEE599 pKa = 4.52 SKK601 pKa = 10.71 SININNCEE609 pKa = 3.93 NATITIQNSNSVIIEE624 pKa = 4.01 KK625 pKa = 10.39 ADD627 pKa = 4.01 NISDD631 pKa = 3.61 VSEE634 pKa = 3.94 ICIKK638 pKa = 10.9 NSIIDD643 pKa = 3.77 VINIYY648 pKa = 9.89 SPCTLSLRR656 pKa = 11.84 IDD658 pKa = 3.35 NCNINEE664 pKa = 4.02 IQAVCDD670 pKa = 4.25 DD671 pKa = 3.93 GVLTIEE677 pKa = 5.16 NSIIQNNIVSSFYY690 pKa = 11.19 TNIIRR695 pKa = 11.84 NNVMLHH701 pKa = 4.93 TAYY704 pKa = 9.8 ISFVQSGIYY713 pKa = 9.72 KK714 pKa = 9.96 ILEE717 pKa = 4.15 APKK720 pKa = 9.57 TAIIDD725 pKa = 3.55 NNIIGEE731 pKa = 4.34 VILDD735 pKa = 3.65 EE736 pKa = 6.15 FEE738 pKa = 4.58 FLDD741 pKa = 5.06 DD742 pKa = 3.8 FTTAIPIIFEE752 pKa = 4.04 FYY754 pKa = 10.76 GYY756 pKa = 9.72 IPHH759 pKa = 6.69 YY760 pKa = 9.87 NFSFIINNTNVDD772 pKa = 4.38 LNIQNDD778 pKa = 3.44 NKK780 pKa = 10.47 FAGIISNSNVYY791 pKa = 10.44 LHH793 pKa = 6.54 LHH795 pKa = 5.66 SLNTALEE802 pKa = 4.44 TYY804 pKa = 10.52 EE805 pKa = 4.7 NVLLYY810 pKa = 8.88 EE811 pKa = 4.56 TFLHH815 pKa = 7.22 LYY817 pKa = 8.89 NCVVEE822 pKa = 4.33 NSPNSEE828 pKa = 3.9 VSSSDD833 pKa = 3.37 DD834 pKa = 3.34 YY835 pKa = 11.78 CIISSTLTFDD845 pKa = 2.92 NCYY848 pKa = 10.87 ANNLGTIVGYY858 pKa = 5.9 EE859 pKa = 3.69 TSYY862 pKa = 11.05 IRR864 pKa = 11.84 TNNSPISVNEE874 pKa = 3.8 DD875 pKa = 2.94 EE876 pKa = 5.29 LLYY879 pKa = 11.08 GHH881 pKa = 7.24 TYY883 pKa = 10.34 DD884 pKa = 3.53 ISRR887 pKa = 11.84 GTVINTEE894 pKa = 3.92 NKK896 pKa = 10.19 LGISRR901 pKa = 11.84 GRR903 pKa = 11.84 NKK905 pKa = 10.45 EE906 pKa = 4.05 PLWLEE911 pKa = 3.92
Molecular weight: 103.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.065
IPC2_protein 4.291
IPC_protein 4.279
Toseland 4.101
ProMoST 4.368
Dawson 4.228
Bjellqvist 4.38
Wikipedia 4.101
Rodwell 4.101
Grimsley 4.012
Solomon 4.228
Lehninger 4.177
Nozaki 4.329
DTASelect 4.495
Thurlkill 4.101
EMBOSS 4.113
Sillero 4.38
Patrickios 0.947
IPC_peptide 4.228
IPC2_peptide 4.368
IPC2.peptide.svr19 4.291
Protein with the highest isoelectric point:
>tr|A0A345BNZ9|A0A345BNZ9_9CAUD Uncharacterized protein OS=CrAssphage sp. OX=2212563 PE=4 SV=1
MM1 pKa = 7.52 NIAIRR6 pKa = 11.84 YY7 pKa = 7.09 KK8 pKa = 10.68 FKK10 pKa = 10.75 FNKK13 pKa = 8.15 ITIFTICINLLINISISIYY32 pKa = 9.28 YY33 pKa = 8.44 LHH35 pKa = 7.25 KK36 pKa = 10.54 SIYY39 pKa = 7.95 SKK41 pKa = 10.92 KK42 pKa = 10.03 IIRR45 pKa = 11.84 STNMMTKK52 pKa = 9.91 HH53 pKa = 6.03 YY54 pKa = 10.04 IASRR58 pKa = 11.84 YY59 pKa = 9.58 NKK61 pKa = 9.69 FIFYY65 pKa = 10.39 IKK67 pKa = 10.02 ILYY70 pKa = 9.21 KK71 pKa = 10.58 CIISFSTFNKK81 pKa = 9.43 NRR83 pKa = 11.84 NNTYY87 pKa = 8.41 TSIFIVSFSRR97 pKa = 11.84 KK98 pKa = 8.17 NNSRR102 pKa = 11.84 KK103 pKa = 9.25 FFSKK107 pKa = 10.5 NSSNKK112 pKa = 9.39 TIIRR116 pKa = 11.84 SNIYY120 pKa = 9.22 SRR122 pKa = 11.84 CNISYY127 pKa = 10.65 ILFCNLINRR136 pKa = 11.84 CWKK139 pKa = 10.17 INIKK143 pKa = 10.43 FSVLTFYY150 pKa = 10.9 IKK152 pKa = 9.86 MYY154 pKa = 10.83 HH155 pKa = 6.58 NINLINNKK163 pKa = 9.77 YY164 pKa = 9.97 NILFIFIIILYY175 pKa = 9.69 NILYY179 pKa = 9.61 YY180 pKa = 10.1 II181 pKa = 4.5
Molecular weight: 22.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.262
IPC2_protein 10.175
IPC_protein 10.306
Toseland 10.292
ProMoST 10.014
Dawson 10.511
Bjellqvist 10.204
Wikipedia 10.687
Rodwell 11.008
Grimsley 10.584
Solomon 10.526
Lehninger 10.482
Nozaki 10.292
DTASelect 10.189
Thurlkill 10.35
EMBOSS 10.701
Sillero 10.423
Patrickios 10.613
IPC_peptide 10.511
IPC2_peptide 9.136
IPC2.peptide.svr19 8.383
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
80
0
80
27590
81
4253
344.9
39.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.625 ± 0.353
1.225 ± 0.185
6.292 ± 0.184
6.524 ± 0.296
4.215 ± 0.147
5.259 ± 0.26
1.591 ± 0.103
8.499 ± 0.418
8.307 ± 0.306
7.506 ± 0.176
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.222 ± 0.145
8.311 ± 0.26
2.588 ± 0.129
3.226 ± 0.241
3.987 ± 0.14
6.557 ± 0.198
6.444 ± 0.23
5.676 ± 0.205
0.819 ± 0.086
5.125 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here