Streptococcus satellite phage Javan147
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZK54|A0A4D5ZK54_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan147 OX=2558541 GN=JavanS147_0010 PE=4 SV=1
MM1 pKa = 7.93 DD2 pKa = 5.55 FSDD5 pKa = 5.66 FLNKK9 pKa = 9.27 KK10 pKa = 8.17 QKK12 pKa = 10.03 EE13 pKa = 3.92 WDD15 pKa = 3.75 EE16 pKa = 3.95 FHH18 pKa = 7.54 PIPDD22 pKa = 4.1 FSMMSDD28 pKa = 3.67 EE29 pKa = 4.28 EE30 pKa = 4.98 LLYY33 pKa = 10.89 QPMSEE38 pKa = 4.18 ALVSEE43 pKa = 4.67 KK44 pKa = 10.12 FAKK47 pKa = 10.04 EE48 pKa = 3.65 LSS50 pKa = 3.62
Molecular weight: 5.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.503
IPC2_protein 4.355
IPC_protein 4.19
Toseland 4.024
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.037
Rodwell 4.037
Grimsley 3.948
Solomon 4.139
Lehninger 4.088
Nozaki 4.266
DTASelect 4.406
Thurlkill 4.062
EMBOSS 4.05
Sillero 4.304
Patrickios 3.999
IPC_peptide 4.139
IPC2_peptide 4.291
IPC2.peptide.svr19 4.225
Protein with the highest isoelectric point:
>tr|A0A4D5ZFS0|A0A4D5ZFS0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan147 OX=2558541 GN=JavanS147_0008 PE=4 SV=1
MM1 pKa = 7.91 KK2 pKa = 9.41 YY3 pKa = 8.38 TLTGMEE9 pKa = 4.11 QLEE12 pKa = 4.61 KK13 pKa = 10.46 RR14 pKa = 11.84 LQSINEE20 pKa = 4.02 INWQKK25 pKa = 10.61 VANKK29 pKa = 10.21 NLTEE33 pKa = 4.05 MFNRR37 pKa = 11.84 AARR40 pKa = 11.84 PPGTPIGKK48 pKa = 7.95 NTKK51 pKa = 8.97 KK52 pKa = 10.18 HH53 pKa = 5.39 SAGEE57 pKa = 3.93 LMRR60 pKa = 11.84 SRR62 pKa = 11.84 RR63 pKa = 11.84 LNRR66 pKa = 11.84 VNISKK71 pKa = 9.03 GHH73 pKa = 5.4 WLGEE77 pKa = 3.61 FGYY80 pKa = 10.18 IKK82 pKa = 10.36 DD83 pKa = 3.96 YY84 pKa = 11.0 APHH87 pKa = 6.57 VEE89 pKa = 4.05 YY90 pKa = 10.65 GHH92 pKa = 6.96 RR93 pKa = 11.84 IVRR96 pKa = 11.84 NGKK99 pKa = 8.29 QVGYY103 pKa = 11.31 VNGTKK108 pKa = 10.65 YY109 pKa = 10.86 LFNNVKK115 pKa = 9.38 KK116 pKa = 10.39 QRR118 pKa = 11.84 EE119 pKa = 4.35 IYY121 pKa = 10.35 RR122 pKa = 11.84 QDD124 pKa = 3.26 LLQAIKK130 pKa = 11.03 NNGKK134 pKa = 8.42 GTVSSGSSSSLDD146 pKa = 3.49 LLKK149 pKa = 9.83 TAKK152 pKa = 10.16 NLNQTYY158 pKa = 9.95 QRR160 pKa = 11.84 VYY162 pKa = 10.65 SPNGKK167 pKa = 9.72 LEE169 pKa = 4.14 FKK171 pKa = 10.28 PVSKK175 pKa = 10.64 SNRR178 pKa = 11.84 LISLAKK184 pKa = 9.0 KK185 pKa = 8.7 TYY187 pKa = 9.8 KK188 pKa = 10.45 QLDD191 pKa = 3.76 DD192 pKa = 3.72
Molecular weight: 22.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.212
IPC2_protein 9.75
IPC_protein 9.882
Toseland 10.482
ProMoST 10.058
Dawson 10.599
Bjellqvist 10.233
Wikipedia 10.745
Rodwell 11.184
Grimsley 10.657
Solomon 10.628
Lehninger 10.599
Nozaki 10.438
DTASelect 10.233
Thurlkill 10.482
EMBOSS 10.847
Sillero 10.511
Patrickios 10.877
IPC_peptide 10.628
IPC2_peptide 8.756
IPC2.peptide.svr19 8.766
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
0
14
2176
50
381
155.4
18.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.147 ± 0.566
0.643 ± 0.234
5.101 ± 0.591
10.386 ± 1.401
3.814 ± 0.394
3.493 ± 0.581
1.517 ± 0.262
7.123 ± 0.433
10.754 ± 0.518
10.478 ± 0.397
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.206 ± 0.237
6.664 ± 0.804
2.16 ± 0.304
4.825 ± 0.419
4.55 ± 0.614
6.664 ± 0.459
5.055 ± 0.447
4.136 ± 0.682
0.735 ± 0.144
4.55 ± 0.443
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here