Acidianus hospitalis (strain W1)
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2329 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F4B676|F4B676_ACIHW 4-hydroxybenzoate polyprenyltransferase OS=Acidianus hospitalis (strain W1) OX=933801 GN=Ahos_0476 PE=4 SV=1
MM1 pKa = 7.2 AQAEE5 pKa = 4.54 FVFEE9 pKa = 4.17 EE10 pKa = 4.46 TVRR13 pKa = 11.84 IYY15 pKa = 10.47 DD16 pKa = 3.56 TDD18 pKa = 3.71 TQGIAHH24 pKa = 6.3 YY25 pKa = 10.56 ASYY28 pKa = 11.42
Molecular weight: 3.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.04
IPC2_protein 4.368
IPC_protein 4.024
Toseland 3.884
ProMoST 4.202
Dawson 3.999
Bjellqvist 4.177
Wikipedia 3.948
Rodwell 3.884
Grimsley 3.821
Solomon 3.961
Lehninger 3.923
Nozaki 4.164
DTASelect 4.24
Thurlkill 3.961
EMBOSS 3.948
Sillero 4.151
Patrickios 0.477
IPC_peptide 3.961
IPC2_peptide 4.126
IPC2.peptide.svr19 4.062
Protein with the highest isoelectric point:
>tr|F4B464|F4B464_ACIHW Translation initiation factor 6 OS=Acidianus hospitalis (strain W1) OX=933801 GN=eif6 PE=3 SV=1
MM1 pKa = 7.24 STTKK5 pKa = 10.4 PLAKK9 pKa = 10.1 KK10 pKa = 10.25 LRR12 pKa = 11.84 LAKK15 pKa = 10.29 ALKK18 pKa = 10.3 SNSAIPAWVILKK30 pKa = 9.3 TNGKK34 pKa = 9.17 VRR36 pKa = 11.84 INPLRR41 pKa = 11.84 RR42 pKa = 11.84 NWRR45 pKa = 11.84 RR46 pKa = 11.84 NSLKK50 pKa = 10.65 VV51 pKa = 3.18
Molecular weight: 5.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 10.994
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.618
Grimsley 12.764
Solomon 13.203
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.34
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 9.019
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2329
0
2329
620352
28
1321
266.4
30.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.79 ± 0.049
0.791 ± 0.02
4.806 ± 0.043
7.306 ± 0.06
4.501 ± 0.048
6.499 ± 0.049
1.277 ± 0.016
9.778 ± 0.052
8.289 ± 0.061
9.994 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.243 ± 0.023
4.708 ± 0.04
3.881 ± 0.039
2.031 ± 0.025
4.032 ± 0.041
6.777 ± 0.053
4.536 ± 0.043
7.091 ± 0.043
0.963 ± 0.02
4.707 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here