Succinatimonas hippei (strain DSM 22608 / JCM 16073 / KCTC 15190 / YIT 12066)
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2169 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E8LLN0|E8LLN0_SUCHY ATP synthase F(1) sector subunit delta OS=Succinatimonas hippei (strain DSM 22608 / JCM 16073 / KCTC 15190 / YIT 12066) OX=762983 GN=atpH PE=4 SV=1
MM1 pKa = 7.66 HH2 pKa = 7.5 EE3 pKa = 4.36 FLGDD7 pKa = 3.45 QDD9 pKa = 4.28 ISAFDD14 pKa = 3.42 NTGTLDD20 pKa = 3.39 TTYY23 pKa = 11.03 EE24 pKa = 4.82 NDD26 pKa = 3.61 DD27 pKa = 2.63 TWYY30 pKa = 10.47 NVGVGLSVMSSQNTYY45 pKa = 10.39 FFIEE49 pKa = 4.26 KK50 pKa = 9.02 EE51 pKa = 4.05 QSFGADD57 pKa = 3.04 NEE59 pKa = 4.31 DD60 pKa = 4.33 TYY62 pKa = 11.57 TVAGGFRR69 pKa = 11.84 HH70 pKa = 6.14 NFF72 pKa = 3.22
Molecular weight: 8.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.795
IPC_protein 3.706
Toseland 3.516
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.439
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.846
Patrickios 0.693
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|E8LHY7|E8LHY7_SUCHY TRAP transporter DctM subunit OS=Succinatimonas hippei (strain DSM 22608 / JCM 16073 / KCTC 15190 / YIT 12066) OX=762983 GN=HMPREF9444_00300 PE=4 SV=1
MM1 pKa = 7.48 LGIGRR6 pKa = 11.84 TLLLQLFVVISKK18 pKa = 10.86 SSLFKK23 pKa = 10.73 KK24 pKa = 9.93 LRR26 pKa = 11.84 PRR28 pKa = 11.84 FGQNRR33 pKa = 11.84 QNIARR38 pKa = 11.84 RR39 pKa = 11.84 QKK41 pKa = 9.92 II42 pKa = 3.35
Molecular weight: 4.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.445
IPC2_protein 10.965
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.457
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.193
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2169
0
2169
675290
29
1823
311.3
34.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.297 ± 0.061
1.498 ± 0.024
5.741 ± 0.045
5.969 ± 0.05
4.784 ± 0.041
6.616 ± 0.055
1.834 ± 0.026
7.514 ± 0.045
6.983 ± 0.059
10.226 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.497 ± 0.029
4.821 ± 0.046
3.677 ± 0.031
3.025 ± 0.027
4.261 ± 0.036
6.516 ± 0.045
4.876 ± 0.038
6.694 ± 0.052
0.781 ± 0.018
3.391 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here