Amino acid dipepetide frequency for Streptomyces natalensis ATCC 27448

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.585AlaAla: 22.585 ± 0.181	1.138AlaCys: 1.138 ± 0.026	8.425AlaAsp: 8.425 ± 0.078	8.673AlaGlu: 8.673 ± 0.094	3.598AlaPhe: 3.598 ± 0.05	12.825AlaGly: 12.825 ± 0.093	3.183AlaHis: 3.183 ± 0.044	3.596AlaIle: 3.596 ± 0.049	3.132AlaLys: 3.132 ± 0.047	14.889AlaLeu: 14.889 ± 0.115	2.687AlaMet: 2.687 ± 0.035	1.927AlaAsn: 1.927 ± 0.032	7.522AlaPro: 7.522 ± 0.092	4.118AlaGln: 4.118 ± 0.043	10.37AlaArg: 10.37 ± 0.087	5.692AlaSer: 5.692 ± 0.056	7.113AlaThr: 7.113 ± 0.06	11.947AlaVal: 11.947 ± 0.094	1.798AlaTrp: 1.798 ± 0.032	2.75AlaTyr: 2.75 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	1.198CysAla: 1.198 ± 0.03	0.1CysCys: 0.1 ± 0.008	0.5CysAsp: 0.5 ± 0.017	0.403CysGlu: 0.403 ± 0.013	0.235CysPhe: 0.235 ± 0.01	1.04CysGly: 1.04 ± 0.027	0.232CysHis: 0.232 ± 0.012	0.225CysIle: 0.225 ± 0.011	0.134CysLys: 0.134 ± 0.008	0.798CysLeu: 0.798 ± 0.02	0.125CysMet: 0.125 ± 0.007	0.157CysAsn: 0.157 ± 0.009	0.533CysPro: 0.533 ± 0.018	0.209CysGln: 0.209 ± 0.01	0.661CysArg: 0.661 ± 0.019	0.448CysSer: 0.448 ± 0.015	0.578CysThr: 0.578 ± 0.019	0.635CysVal: 0.635 ± 0.02	0.132CysTrp: 0.132 ± 0.008	0.177CysTyr: 0.177 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.864AspAla: 7.864 ± 0.074	0.451AspCys: 0.451 ± 0.014	3.586AspAsp: 3.586 ± 0.046	3.739AspGlu: 3.739 ± 0.051	1.614AspPhe: 1.614 ± 0.031	6.361AspGly: 6.361 ± 0.062	1.512AspHis: 1.512 ± 0.027	1.97AspIle: 1.97 ± 0.036	1.265AspLys: 1.265 ± 0.028	6.275AspLeu: 6.275 ± 0.061	0.84AspMet: 0.84 ± 0.019	1.001AspAsn: 1.001 ± 0.026	4.429AspPro: 4.429 ± 0.051	1.578AspGln: 1.578 ± 0.031	4.802AspArg: 4.802 ± 0.054	2.352AspSer: 2.352 ± 0.033	3.132AspThr: 3.132 ± 0.036	4.253AspVal: 4.253 ± 0.048	1.011AspTrp: 1.011 ± 0.025	1.135AspTyr: 1.135 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	7.359GluAla: 7.359 ± 0.088	0.379GluCys: 0.379 ± 0.011	2.728GluAsp: 2.728 ± 0.043	3.446GluGlu: 3.446 ± 0.057	1.441GluPhe: 1.441 ± 0.025	4.25GluGly: 4.25 ± 0.043	1.508GluHis: 1.508 ± 0.033	2.304GluIle: 2.304 ± 0.037	1.411GluLys: 1.411 ± 0.03	6.671GluLeu: 6.671 ± 0.065	0.977GluMet: 0.977 ± 0.024	0.95GluAsn: 0.95 ± 0.023	3.133GluPro: 3.133 ± 0.044	2.201GluGln: 2.201 ± 0.031	5.504GluArg: 5.504 ± 0.056	2.418GluSer: 2.418 ± 0.036	2.817GluThr: 2.817 ± 0.039	4.397GluVal: 4.397 ± 0.055	0.766GluTrp: 0.766 ± 0.023	1.041GluTyr: 1.041 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.737PheAla: 3.737 ± 0.044	0.292PheCys: 0.292 ± 0.011	1.903PheAsp: 1.903 ± 0.035	1.34PheGlu: 1.34 ± 0.026	0.885PhePhe: 0.885 ± 0.024	3.027PheGly: 3.027 ± 0.035	0.671PheHis: 0.671 ± 0.018	0.78PheIle: 0.78 ± 0.021	0.554PheLys: 0.554 ± 0.017	2.6PheLeu: 2.6 ± 0.045	0.398PheMet: 0.398 ± 0.015	0.55PheAsn: 0.55 ± 0.019	1.429PhePro: 1.429 ± 0.023	0.672PheGln: 0.672 ± 0.019	1.825PheArg: 1.825 ± 0.033	1.465PheSer: 1.465 ± 0.026	1.951PheThr: 1.951 ± 0.03	2.104PheVal: 2.104 ± 0.036	0.414PheTrp: 0.414 ± 0.015	0.57PheTyr: 0.57 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	11.244GlyAla: 11.244 ± 0.093	0.897GlyCys: 0.897 ± 0.023	5.009GlyAsp: 5.009 ± 0.055	5.03GlyGlu: 5.03 ± 0.059	2.817GlyPhe: 2.817 ± 0.037	8.931GlyGly: 8.931 ± 0.102	2.496GlyHis: 2.496 ± 0.038	3.699GlyIle: 3.699 ± 0.044	2.599GlyLys: 2.599 ± 0.039	9.36GlyLeu: 9.36 ± 0.093	2.122GlyMet: 2.122 ± 0.033	1.772GlyAsn: 1.772 ± 0.03	5.286GlyPro: 5.286 ± 0.066	2.802GlyGln: 2.802 ± 0.043	7.692GlyArg: 7.692 ± 0.072	5.202GlySer: 5.202 ± 0.054	6.163GlyThr: 6.163 ± 0.067	6.996GlyVal: 6.996 ± 0.06	1.704GlyTrp: 1.704 ± 0.031	2.248GlyTyr: 2.248 ± 0.031	0.0GlyXaa: 0.0 ± 0.0
His	2.877HisAla: 2.877 ± 0.04	0.24HisCys: 0.24 ± 0.012	1.458HisAsp: 1.458 ± 0.03	1.199HisGlu: 1.199 ± 0.023	0.709HisPhe: 0.709 ± 0.018	2.582HisGly: 2.582 ± 0.039	0.802HisHis: 0.802 ± 0.023	0.8HisIle: 0.8 ± 0.02	0.442HisLys: 0.442 ± 0.015	2.597HisLeu: 2.597 ± 0.035	0.376HisMet: 0.376 ± 0.015	0.405HisAsn: 0.405 ± 0.014	2.002HisPro: 2.002 ± 0.029	0.796HisGln: 0.796 ± 0.018	2.249HisArg: 2.249 ± 0.036	1.101HisSer: 1.101 ± 0.026	1.563HisThr: 1.563 ± 0.027	1.628HisVal: 1.628 ± 0.03	0.383HisTrp: 0.383 ± 0.015	0.481HisTyr: 0.481 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	5.12IleAla: 5.12 ± 0.051	0.344IleCys: 0.344 ± 0.013	2.355IleAsp: 2.355 ± 0.036	2.133IleGlu: 2.133 ± 0.033	0.723IlePhe: 0.723 ± 0.019	3.737IleGly: 3.737 ± 0.054	0.662IleHis: 0.662 ± 0.019	0.931IleIle: 0.931 ± 0.022	0.819IleLys: 0.819 ± 0.021	2.415IleLeu: 2.415 ± 0.04	0.469IleMet: 0.469 ± 0.016	0.773IleAsn: 0.773 ± 0.019	1.955IlePro: 1.955 ± 0.034	0.763IleGln: 0.763 ± 0.018	2.415IleArg: 2.415 ± 0.039	1.705IleSer: 1.705 ± 0.026	2.253IleThr: 2.253 ± 0.035	2.745IleVal: 2.745 ± 0.04	0.41IleTrp: 0.41 ± 0.014	0.556IleTyr: 0.556 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	3.043LysAla: 3.043 ± 0.05	0.118LysCys: 0.118 ± 0.007	1.478LysAsp: 1.478 ± 0.032	1.236LysGlu: 1.236 ± 0.026	0.486LysPhe: 0.486 ± 0.016	1.964LysGly: 1.964 ± 0.035	0.482LysHis: 0.482 ± 0.016	0.988LysIle: 0.988 ± 0.024	1.017LysLys: 1.017 ± 0.037	2.14LysLeu: 2.14 ± 0.035	0.441LysMet: 0.441 ± 0.017	0.594LysAsn: 0.594 ± 0.02	1.417LysPro: 1.417 ± 0.029	0.775LysGln: 0.775 ± 0.021	1.589LysArg: 1.589 ± 0.029	1.189LysSer: 1.189 ± 0.026	1.391LysThr: 1.391 ± 0.031	1.902LysVal: 1.902 ± 0.037	0.28LysTrp: 0.28 ± 0.01	0.463LysTyr: 0.463 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	15.596LeuAla: 15.596 ± 0.11	0.932LeuCys: 0.932 ± 0.026	6.605LeuAsp: 6.605 ± 0.068	4.714LeuGlu: 4.714 ± 0.059	2.603LeuPhe: 2.603 ± 0.037	9.162LeuGly: 9.162 ± 0.08	2.566LeuHis: 2.566 ± 0.035	3.491LeuIle: 3.491 ± 0.045	2.068LeuLys: 2.068 ± 0.037	11.177LeuLeu: 11.177 ± 0.111	1.728LeuMet: 1.728 ± 0.029	1.679LeuAsn: 1.679 ± 0.032	6.645LeuPro: 6.645 ± 0.064	2.282LeuGln: 2.282 ± 0.035	8.723LeuArg: 8.723 ± 0.079	5.248LeuSer: 5.248 ± 0.056	6.938LeuThr: 6.938 ± 0.068	8.334LeuVal: 8.334 ± 0.08	1.289LeuTrp: 1.289 ± 0.025	1.808LeuTyr: 1.808 ± 0.027	0.0LeuXaa: 0.0 ± 0.0
Met	2.497MetAla: 2.497 ± 0.031	0.15MetCys: 0.15 ± 0.009	0.971MetAsp: 0.971 ± 0.02	0.793MetGlu: 0.793 ± 0.02	0.441MetPhe: 0.441 ± 0.017	1.454MetGly: 1.454 ± 0.031	0.406MetHis: 0.406 ± 0.015	0.649MetIle: 0.649 ± 0.018	0.454MetLys: 0.454 ± 0.016	1.786MetLeu: 1.786 ± 0.031	0.33MetMet: 0.33 ± 0.015	0.459MetAsn: 0.459 ± 0.015	1.235MetPro: 1.235 ± 0.028	0.495MetGln: 0.495 ± 0.015	1.46MetArg: 1.46 ± 0.027	1.302MetSer: 1.302 ± 0.025	1.585MetThr: 1.585 ± 0.031	1.333MetVal: 1.333 ± 0.026	0.221MetTrp: 0.221 ± 0.01	0.32MetTyr: 0.32 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.216AsnAla: 2.216 ± 0.029	0.181AsnCys: 0.181 ± 0.01	0.994AsnAsp: 0.994 ± 0.027	0.804AsnGlu: 0.804 ± 0.021	0.48AsnPhe: 0.48 ± 0.014	1.918AsnGly: 1.918 ± 0.033	0.418AsnHis: 0.418 ± 0.016	0.679AsnIle: 0.679 ± 0.018	0.457AsnLys: 0.457 ± 0.015	1.598AsnLeu: 1.598 ± 0.027	0.318AsnMet: 0.318 ± 0.013	0.426AsnAsn: 0.426 ± 0.015	1.329AsnPro: 1.329 ± 0.027	0.507AsnGln: 0.507 ± 0.019	1.26AsnArg: 1.26 ± 0.026	0.9AsnSer: 0.9 ± 0.023	1.111AsnThr: 1.111 ± 0.024	1.334AsnVal: 1.334 ± 0.027	0.299AsnTrp: 0.299 ± 0.01	0.424AsnTyr: 0.424 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	8.727ProAla: 8.727 ± 0.101	0.394ProCys: 0.394 ± 0.012	4.537ProAsp: 4.537 ± 0.051	4.236ProGlu: 4.236 ± 0.05	1.558ProPhe: 1.558 ± 0.029	6.624ProGly: 6.624 ± 0.081	1.577ProHis: 1.577 ± 0.03	1.276ProIle: 1.276 ± 0.026	1.329ProLys: 1.329 ± 0.029	5.483ProLeu: 5.483 ± 0.06	1.057ProMet: 1.057 ± 0.024	0.906ProAsn: 0.906 ± 0.024	3.705ProPro: 3.705 ± 0.072	2.103ProGln: 2.103 ± 0.046	4.021ProArg: 4.021 ± 0.048	3.156ProSer: 3.156 ± 0.048	3.183ProThr: 3.183 ± 0.043	5.361ProVal: 5.361 ± 0.052	0.912ProTrp: 0.912 ± 0.022	1.486ProTyr: 1.486 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.923GlnAla: 3.923 ± 0.043	0.208GlnCys: 0.208 ± 0.01	1.503GlnAsp: 1.503 ± 0.025	1.597GlnGlu: 1.597 ± 0.032	0.716GlnPhe: 0.716 ± 0.018	2.404GlnGly: 2.404 ± 0.041	0.766GlnHis: 0.766 ± 0.019	1.184GlnIle: 1.184 ± 0.027	0.684GlnLys: 0.684 ± 0.023	3.314GlnLeu: 3.314 ± 0.045	0.591GlnMet: 0.591 ± 0.018	0.507GlnAsn: 0.507 ± 0.018	1.792GlnPro: 1.792 ± 0.042	1.511GlnGln: 1.511 ± 0.053	2.561GlnArg: 2.561 ± 0.038	1.291GlnSer: 1.291 ± 0.027	1.397GlnThr: 1.397 ± 0.026	2.36GlnVal: 2.36 ± 0.036	0.528GlnTrp: 0.528 ± 0.017	0.613GlnTyr: 0.613 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	9.831ArgAla: 9.831 ± 0.085	0.628ArgCys: 0.628 ± 0.021	4.145ArgAsp: 4.145 ± 0.043	4.638ArgGlu: 4.638 ± 0.051	2.285ArgPhe: 2.285 ± 0.036	5.849ArgGly: 5.849 ± 0.062	2.209ArgHis: 2.209 ± 0.035	3.518ArgIle: 3.518 ± 0.044	1.801ArgLys: 1.801 ± 0.031	8.815ArgLeu: 8.815 ± 0.071	1.835ArgMet: 1.835 ± 0.03	1.4ArgAsn: 1.4 ± 0.03	5.088ArgPro: 5.088 ± 0.057	2.495ArgGln: 2.495 ± 0.036	7.934ArgArg: 7.934 ± 0.083	4.043ArgSer: 4.043 ± 0.044	5.439ArgThr: 5.439 ± 0.053	5.295ArgVal: 5.295 ± 0.059	1.365ArgTrp: 1.365 ± 0.027	1.853ArgTyr: 1.853 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	6.605SerAla: 6.605 ± 0.054	0.441SerCys: 0.441 ± 0.017	2.54SerAsp: 2.54 ± 0.038	2.263SerGlu: 2.263 ± 0.039	1.51SerPhe: 1.51 ± 0.028	5.764SerGly: 5.764 ± 0.06	1.068SerHis: 1.068 ± 0.026	1.404SerIle: 1.404 ± 0.029	1.075SerLys: 1.075 ± 0.024	4.785SerLeu: 4.785 ± 0.052	1.082SerMet: 1.082 ± 0.022	0.835SerAsn: 0.835 ± 0.021	3.142SerPro: 3.142 ± 0.044	1.311SerGln: 1.311 ± 0.025	3.614SerArg: 3.614 ± 0.037	2.744SerSer: 2.744 ± 0.041	2.799SerThr: 2.799 ± 0.039	4.018SerVal: 4.018 ± 0.05	0.888SerTrp: 0.888 ± 0.025	1.194SerTyr: 1.194 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	8.972ThrAla: 8.972 ± 0.072	0.459ThrCys: 0.459 ± 0.017	3.58ThrAsp: 3.58 ± 0.038	3.112ThrGlu: 3.112 ± 0.037	1.622ThrPhe: 1.622 ± 0.03	6.629ThrGly: 6.629 ± 0.062	1.279ThrHis: 1.279 ± 0.023	1.739ThrIle: 1.739 ± 0.036	1.225ThrLys: 1.225 ± 0.026	5.759ThrLeu: 5.759 ± 0.056	0.966ThrMet: 0.966 ± 0.025	0.989ThrAsn: 0.989 ± 0.022	4.141ThrPro: 4.141 ± 0.043	1.395ThrGln: 1.395 ± 0.027	3.795ThrArg: 3.795 ± 0.044	2.969ThrSer: 2.969 ± 0.038	3.695ThrThr: 3.695 ± 0.055	5.876ThrVal: 5.876 ± 0.053	0.895ThrTrp: 0.895 ± 0.021	1.319ThrTyr: 1.319 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	10.155ValAla: 10.155 ± 0.088	0.811ValCys: 0.811 ± 0.021	4.539ValAsp: 4.539 ± 0.051	4.363ValGlu: 4.363 ± 0.049	2.292ValPhe: 2.292 ± 0.042	6.168ValGly: 6.168 ± 0.068	2.038ValHis: 2.038 ± 0.034	2.991ValIle: 2.991 ± 0.037	1.716ValLys: 1.716 ± 0.037	9.196ValLeu: 9.196 ± 0.077	1.431ValMet: 1.431 ± 0.026	1.56ValAsn: 1.56 ± 0.027	4.996ValPro: 4.996 ± 0.05	2.125ValGln: 2.125 ± 0.033	6.818ValArg: 6.818 ± 0.062	4.032ValSer: 4.032 ± 0.052	5.206ValThr: 5.206 ± 0.052	7.13ValVal: 7.13 ± 0.075	1.074ValTrp: 1.074 ± 0.027	1.458ValTyr: 1.458 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.62TrpAla: 1.62 ± 0.027	0.176TrpCys: 0.176 ± 0.01	0.828TrpAsp: 0.828 ± 0.023	0.741TrpGlu: 0.741 ± 0.018	0.506TrpPhe: 0.506 ± 0.015	1.052TrpGly: 1.052 ± 0.028	0.42TrpHis: 0.42 ± 0.015	0.551TrpIle: 0.551 ± 0.016	0.405TrpLys: 0.405 ± 0.014	1.788TrpLeu: 1.788 ± 0.033	0.328TrpMet: 0.328 ± 0.014	0.387TrpAsn: 0.387 ± 0.014	0.773TrpPro: 0.773 ± 0.02	0.677TrpGln: 0.677 ± 0.017	1.32TrpArg: 1.32 ± 0.025	0.9TrpSer: 0.9 ± 0.024	0.981TrpThr: 0.981 ± 0.022	0.929TrpVal: 0.929 ± 0.022	0.324TrpTrp: 0.324 ± 0.015	0.331TrpTyr: 0.331 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.8TyrAla: 2.8 ± 0.039	0.183TyrCys: 0.183 ± 0.01	1.487TyrAsp: 1.487 ± 0.034	1.246TyrGlu: 1.246 ± 0.028	0.649TyrPhe: 0.649 ± 0.019	2.327TyrGly: 2.327 ± 0.039	0.396TyrHis: 0.396 ± 0.014	0.504TyrIle: 0.504 ± 0.016	0.402TyrLys: 0.402 ± 0.015	2.143TyrLeu: 2.143 ± 0.033	0.239TyrMet: 0.239 ± 0.01	0.377TyrAsn: 0.377 ± 0.016	1.08TyrPro: 1.08 ± 0.023	0.635TyrGln: 0.635 ± 0.018	1.851TyrArg: 1.851 ± 0.036	0.927TyrSer: 0.927 ± 0.023	1.118TyrThr: 1.118 ± 0.025	1.522TyrVal: 1.522 ± 0.033	0.343TyrTrp: 0.343 ± 0.013	0.445TyrTyr: 0.445 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6559 proteins (2045269 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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